Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,445,410 C→A 100% R79L (CGT→CTT)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,445,4100CA98.5% 852.8 / 2.9 269R79L (CGT→CTT) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (134/130);  total (138/131)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

CTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445264‑3445556
                                                                                                                                                  |                                                                                                                                                  
 ttCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCa                                                                                                                                                <  2:1181152/150‑1 (MQ=255)
 ttCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCa                                                                                                                                                <  1:269444/150‑1 (MQ=255)
 ttCTACAGAGAACTTATCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCa                                                                                                                                                <  1:4148660/150‑1 (MQ=255)
  tatACAGAGAACTTCTCGACAACGATCAGAAGATCCTGACGTACCTGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGTCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAg                                                                                                                                               <  1:2486737/148‑1 (MQ=255)
  tCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAg                                                                                                                                               >  1:1107639/1‑150 (MQ=255)
    tACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             >  2:4801619/1‑150 (MQ=255)
    tACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGAt                                                                                                                                             >  1:1548901/1‑150 (MQ=255)
       agagAACTTCTCGACAACGCTCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCgg                                                                                                                                          <  1:2145543/150‑1 (MQ=255)
        gagaACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCggg                                                                                                                                         >  2:4729852/1‑150 (MQ=255)
           aaCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctc                                                                                                                                      <  2:609213/150‑1 (MQ=255)
            aCTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGctct                                                                                                                                     <  1:4891852/150‑1 (MQ=255)
              ttCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTg                                                                                                                                   <  1:759750/150‑1 (MQ=255)
                    aCAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGaa                                                                                                                             >  1:1869712/1‑150 (MQ=255)
                     cAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAc                                                                                                                            <  2:2712472/150‑1 (MQ=255)
                      aaCGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAAcc                                                                                                                           <  2:4368686/150‑1 (MQ=255)
                        cGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAg                                                                                                                         >  1:5203248/1‑150 (MQ=255)
                         gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                        >  1:3928861/1‑150 (MQ=255)
                         gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                        <  1:4830657/150‑1 (MQ=255)
                         gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGa                                                                                                                        <  1:3123673/150‑1 (MQ=255)
                         gATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGATCTTGATAGAACCAGa                                                                                                                        >  2:3507412/1‑150 (MQ=255)
                             aGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgc                                                                                                                    <  1:476270/150‑1 (MQ=255)
                             aGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgc                                                                                                                    >  1:4252819/1‑150 (MQ=255)
                              gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgcg                                                                                                                   >  2:3843943/1‑150 (MQ=255)
                              gACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAcgcg                                                                                                                   <  2:4512040/150‑1 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  2:2153368/1‑150 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  2:1424968/1‑150 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  2:3457003/1‑150 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  1:1913698/1‑150 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  2:1789561/1‑150 (MQ=255)
                                 gATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                                                                >  1:4662309/1‑150 (MQ=255)
                                      tGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCg                                                                                                           <  1:5064194/150‑1 (MQ=255)
                                        aCGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGt                                                                                                         <  2:4983091/150‑1 (MQ=255)
                                         cGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                                                                        <  1:4439888/150‑1 (MQ=255)
                                          gTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                                                                       >  2:5118013/1‑150 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCtt                                                                                                    <  1:431328/150‑1 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCtt                                                                                                    <  2:3279189/150‑1 (MQ=255)
                                             ccAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCtt                                                                                                    <  2:1900399/150‑1 (MQ=255)
                                              cAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                                                                   <  2:2827851/150‑1 (MQ=255)
                                               aGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTc                                                                                                  >  1:2071101/1‑150 (MQ=255)
                                                gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt                                                                                                 >  2:277713/1‑150 (MQ=255)
                                                gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt                                                                                                 <  1:2206712/150‑1 (MQ=255)
                                                gTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt                                                                                                 <  2:293391/150‑1 (MQ=255)
                                                      aCAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                                                           >  2:3617528/1‑150 (MQ=255)
                                                        aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                                                                         >  2:4229291/1‑150 (MQ=255)
                                                         ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                                                                        >  1:3109886/1‑150 (MQ=255)
                                                          gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                                                                       >  2:4110721/1‑150 (MQ=255)
                                                                  tctgcgcgcCGCGGTACTTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAc                                                                               <  2:340079/147‑1 (MQ=255)
                                                                  tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAc                                                                               <  2:3022608/150‑1 (MQ=255)
                                                                  tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAc                                                                               >  1:731994/1‑150 (MQ=255)
                                                                   cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                                                              <  1:969052/150‑1 (MQ=255)
                                                                    aGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCg                                                                             >  1:4967002/1‑150 (MQ=255)
                                                                         gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                                                                        <  1:125709/150‑1 (MQ=255)
                                                                         gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                                                                        <  1:3719972/150‑1 (MQ=255)
                                                                           cgcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTACTGGCGCCACGGTTTTTTACCGGAACCAg                                                                      >  1:591278/1‑150 (MQ=255)
                                                                              ggTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTa                                                                   <  2:3575922/150‑1 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                  <  2:519009/150‑1 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                  <  1:820030/150‑1 (MQ=255)
                                                                               gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                                                                  >  2:3985261/1‑150 (MQ=255)
                                                                                tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGATCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                                                                 >  1:2866401/1‑150 (MQ=255)
                                                                                  cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                                                               >  2:1614697/1‑150 (MQ=255)
                                                                                   aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                                                              >  2:4614752/1‑150 (MQ=255)
                                                                                    tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                                                             >  1:2009561/1‑150 (MQ=255)
                                                                                    tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                                                             >  2:3735112/1‑150 (MQ=255)
                                                                                    tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAg                                                             <  1:562141/150‑1 (MQ=255)
                                                                                     cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc                                                            >  2:1539668/1‑150 (MQ=255)
                                                                                      ttcttGTTAAATTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa                                                           <  1:1704192/150‑1 (MQ=255)
                                                                                        cgtGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCCCg                                                         >  2:2012230/3‑150 (MQ=255)
                                                                                         ttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAAAGCGCACGGCCGGTGACTTTCTGGCGCCACGGTTTTTTACCTGAACCAGTTAATTCAGCACGa                                                        >  1:3231888/1‑150 (MQ=255)
                                                                                          tGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg                                                       >  1:3476586/1‑150 (MQ=255)
                                                                                              aaCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                   <  2:146421/150‑1 (MQ=255)
                                                                                              aaCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtctt                                                   <  1:3800520/150‑1 (MQ=255)
                                                                                               aCTTTTTGATTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc                                                  <  2:3549144/150‑1 (MQ=255)
                                                                                               aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc                                                  <  2:2856982/150‑1 (MQ=255)
                                                                                                cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct                                                 <  1:5141705/150‑1 (MQ=255)
                                                                                                cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct                                                 <  2:4681405/150‑1 (MQ=255)
                                                                                                  ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGa                                               >  1:3034721/1‑150 (MQ=255)
                                                                                                    ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGc                                             >  2:4304076/1‑150 (MQ=255)
                                                                                                    ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGc                                             >  1:4031910/1‑150 (MQ=255)
                                                                                                        cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGa                                         <  2:4272586/150‑1 (MQ=255)
                                                                                                              tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGAAAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTAccc                                   <  1:683665/150‑1 (MQ=255)
                                                                                                               ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCt                                  <  1:4634626/150‑1 (MQ=255)
                                                                                                                  gCGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGAc                               <  1:2383245/150‑1 (MQ=255)
                                                                                                                   cGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACg                              <  2:3127565/150‑1 (MQ=255)
                                                                                                                   cGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACg                              <  2:5240215/150‑1 (MQ=255)
                                                                                                                   cGGACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCGTTCTGGCGCCACGGTTTTTTACCGGAACCAGGTACTTCAGCACGAGTCTGCTGAGCACGAGTGCCCTGACg                              >  2:2234982/1‑150 (MQ=255)
                                                                                                                    ggACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             >  2:1066755/1‑150 (MQ=255)
                                                                                                                    ggACGAGCAGCAAAGGTAACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTCCTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGa                             >  2:2524474/1‑150 (MQ=255)
                                                                                                                     gACGAGCAGCAAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAg                            >  1:3076088/1‑150 (MQ=255)
                                                                                                                           cagcaATGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAg                      <  1:2952801/150‑1 (MQ=255)
                                                                                                                             gcaAAGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCt                    >  1:3659001/1‑150 (MQ=255)
                                                                                                                               aaaGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGACCCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGc                  >  1:1718956/1‑150 (MQ=255)
                                                                                                                                 aGGTCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCAt                >  2:2456975/1‑150 (MQ=255)
                                                                                                                                   gTCACGCCACCAGAAAGCCAGATCGGGCTATTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCGTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATaa              <  1:5298705/150‑1 (MQ=255)
                                                                                                                                    tCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAg             >  1:3826292/1‑150 (MQ=255)
                                                                                                                                    tCACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAg             >  1:678542/1‑150 (MQ=255)
                                                                                                                                     cACGCCACCAGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGc            >  2:849123/1‑150 (MQ=255)
                                                                                                                                         ccaccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaaca        <  2:3523404/150‑1 (MQ=255)
                                                                                                                                           accaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaac      >  1:1757534/1‑150 (MQ=255)
                                                                                                                                            ccaGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaaca     <  2:1310696/150‑1 (MQ=255)
                                                                                                                                             caGAAAGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGcaacaacaa    >  2:2424793/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTCTACAGAGAACTTCTCGACAACGATCAGACGATCCTGACGTACCAGTTCGGACAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACC  >  CP009273/3445264‑3445556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑