Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,470,272 C→A 100% V169V (GTG→GTT)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,470,2720CA99.7% 1027.6 / ‑4.5 320V169V (GTG→GTT) fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (159/160);  total (159/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 320 total aligned reads displayed.

AACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGC  >  CP009273/3470125‑3470418
                                                                                                                                                   |                                                                                                                                                  
    aGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTaca                                                                                                                                              <  2:4830518/150‑1 (MQ=255)
       ttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGAGAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaaca                                                                                                                                           <  2:4651942/150‑1 (MQ=255)
         gATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGt                                                                                                                                         <  1:1874715/150‑1 (MQ=255)
         gATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGt                                                                                                                                         >  2:3186120/1‑150 (MQ=255)
          aTCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                        >  2:4103114/1‑150 (MQ=255)
            cctACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGAGAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATc                                                                                                                                      <  2:4935468/148‑1 (MQ=255)
            cTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATc                                                                                                                                      <  1:4541908/150‑1 (MQ=255)
              tACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGc                                                                                                                                    >  1:483077/1‑150 (MQ=255)
              tACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGc                                                                                                                                    >  2:5211531/1‑150 (MQ=255)
               aCCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCt                                                                                                                                   >  1:3728112/1‑150 (MQ=255)
                   ccTTTCTTGCTGGTCTTCAGACCTTCTGTCCCACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                               <  1:1601260/150‑1 (MQ=255)
                    cTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCtt                                                                                                                              >  1:4166315/1‑150 (MQ=255)
                     tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCttt                                                                                                                             <  2:4242862/150‑1 (MQ=255)
                       tCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCg                                                                                                                           <  1:325589/150‑1 (MQ=255)
                        cTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCgg                                                                                                                          >  1:4324386/1‑150 (MQ=255)
                           gATGTTCTTCANACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGc                                                                                                                       >  1:2758540/1‑150 (MQ=255)
                            aTGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTATTTATTTAACTAAAACAGTATCGCTTTATTTCGGTGCt                                                                                                                      >  1:2433565/1‑150 (MQ=255)
                              gTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTc                                                                                                                    <  1:4130901/150‑1 (MQ=255)
                                    cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                              >  2:4993854/1‑150 (MQ=255)
                                    cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                              >  2:3051313/1‑150 (MQ=255)
                                      gACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGAGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTATGTAGTTAAATACAACAGTATCGCTGTCTTTAGGTGCTTCGCCTTTAc                                                                                                            >  2:2195383/1‑150 (MQ=255)
                                       aCCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                           <  2:4442309/150‑1 (MQ=255)
                                        ccTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCg                                                                                                          >  1:266599/1‑150 (MQ=255)
                                          ttCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGc                                                                                                        >  1:1003215/1‑150 (MQ=255)
                                          ttCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCAATGAAGTTAACTACAACAGTATCGCTGTCGTTCGGTGATGCGCCGTTACAGGc                                                                                                        >  2:2819198/1‑150 (MQ=255)
                                            cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                      >  2:1957171/1‑150 (MQ=255)
                                             tGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTACGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTc                                                                                                     >  2:2769233/1‑150 (MQ=255)
                                              gTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTct                                                                                                    <  1:4629858/150‑1 (MQ=255)
                                               tCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTcta                                                                                                   >  1:2240118/1‑150 (MQ=255)
                                                  aaCCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTctacta                                                                                                >  1:1252354/1‑150 (MQ=255)
                                                  aaCCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTctacta                                                                                                <  2:1348663/150‑1 (MQ=255)
                                                    cccGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTAcc                                                                                              <  2:2001458/150‑1 (MQ=255)
                                                       gggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGa                                                                                           >  2:4189700/1‑150 (MQ=255)
                                                        ggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGAt                                                                                          <  1:5136004/150‑1 (MQ=255)
                                                        ggATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGAt                                                                                          <  2:2410742/150‑1 (MQ=255)
                                                         gATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCATTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATa                                                                                         >  2:2884999/1‑150 (MQ=255)
                                                          aTAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCAGATaa                                                                                        >  1:1650795/1‑150 (MQ=255)
                                                            aaCACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAac                                                                                      <  1:4502444/150‑1 (MQ=255)
                                                             acacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAacc                                                                                     <  1:4268386/150‑1 (MQ=255)
                                                             acacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCAGTCTTTCGGTGCTTCGCCTTTACCGGATTCTACTACCTGATAAacc                                                                                     >  2:3370804/1‑150 (MQ=255)
                                                              cacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAacca                                                                                    <  2:4355845/150‑1 (MQ=255)
                                                               acCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccag                                                                                   <  2:2318571/150‑1 (MQ=255)
                                                               acCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccag                                                                                   >  2:1793048/1‑150 (MQ=255)
                                                               acCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTAAAACAGTATAGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTAATACCTTATAAaccag                                                                                   >  1:622645/1‑150 (MQ=255)
                                                                  gTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagacc                                                                                >  1:3337479/1‑150 (MQ=255)
                                                                    ccAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                              >  2:4696653/1‑150 (MQ=255)
                                                                    ccAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTGTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagaccag                                                                              >  2:835674/1‑150 (MQ=255)
                                                                    ccAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGGATCGCTGTCGTTCGGTGCTTCGCCTGTACCGGCTTCTACTACCTGATTAaccagacccg                                                                              >  2:4582397/1‑148 (MQ=255)
                                                                          ggAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTAACTGATAAACCAGACCAGTTGAag                                                                        >  2:1633958/1‑150 (MQ=255)
                                                                          ggAAAGAAAGCGGTTAACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTAGATCAGCCAACCTTTGTATTTAACTACAACAGTATAGCAGTCTTTCGGTGCTTCGCCTTTACAGGCTACTACTAACAGATAAACAAGAACAGTTGAag                                                                        >  1:1186413/1‑150 (MQ=255)
                                                                           gaaagaaaGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACAACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCAGATAAACCAGACCAGTTGAaga                                                                       >  1:1868540/1‑150 (MQ=255)
                                                                              agaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGt                                                                    >  1:862415/1‑150 (MQ=255)
                                                                               gaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtt                                                                   <  1:967275/150‑1 (MQ=255)
                                                                                aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                  <  1:1411475/150‑1 (MQ=255)
                                                                                aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                  <  1:3708932/150‑1 (MQ=255)
                                                                                      gTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCacac                                                            <  1:1364013/150‑1 (MQ=255)
                                                                                         caccacGGGTGTTAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCATTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCTGACCTGTTTTAGAGGTTCTCACACCtt                                                         <  2:2657977/150‑1 (MQ=255)
                                                                                         caccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCGTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCtt                                                         >  1:2001727/1‑150 (MQ=255)
                                                                                         caccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGGCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACAccgt                                                         >  1:3557716/1‑148 (MQ=255)
                                                                                           ccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtc                                                       <  1:5117466/150‑1 (MQ=255)
                                                                                           ccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtc                                                       <  1:2518880/150‑1 (MQ=255)
                                                                                           ccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtc                                                       <  1:3903130/150‑1 (MQ=255)
                                                                                            cacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtct                                                      >  1:279940/1‑150 (MQ=255)
                                                                                             acGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAACGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTtctc                                                     >  2:110724/1‑150 (MQ=255)
                                                                                              cGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTctct                                                    <  2:2789079/150‑1 (MQ=255)
                                                                                               gggTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCCGACCAGTTGAAGAGGTTTTCACACCTTTCTCtt                                                   >  2:1593997/1‑150 (MQ=255)
                                                                                                ggTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCttt                                                  <  1:316751/150‑1 (MQ=255)
                                                                                                 gtgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                 <  2:4843317/150‑1 (MQ=255)
                                                                                                 gtgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                 <  2:2597998/150‑1 (MQ=255)
                                                                                                 gtgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGGAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCGTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                 <  1:4920217/150‑1 (MQ=255)
                                                                                                    tAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTTTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCa                                              >  1:105325/1‑150 (MQ=255)
                                                                                                    tAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCa                                              >  1:4009260/1‑150 (MQ=255)
                                                                                                      agagTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaaa                                            >  2:3887835/1‑150 (MQ=255)
                                                                                                        agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAtt                                          <  2:2626536/150‑1 (MQ=255)
                                                                                                            gTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTtctc                                      <  1:1327187/150‑1 (MQ=255)
                                                                                                             tCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                     >  2:4037752/1‑150 (MQ=255)
                                                                                                                aaCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCgg                                  <  2:614427/150‑1 (MQ=255)
                                                                                                                 tctctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAAAAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGt                                 <  1:2342306/149‑1 (MQ=255)
                                                                                                                 aCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGt                                 <  1:2773572/150‑1 (MQ=255)
                                                                                                                  ctctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTa                                >  2:1570842/1‑150 (MQ=255)
                                                                                                                   tctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTAc                               <  2:4869577/150‑1 (MQ=255)
                                                                                                                      ttACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTActct                            <  2:245199/150‑1 (MQ=255)
                                                                                                                        aCCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCttt                          <  1:3374382/150‑1 (MQ=255)
                                                                                                                             cGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtt                     <  1:2202198/150‑1 (MQ=255)
                                                                                                                              gATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCttt                    <  1:1388295/150‑1 (MQ=255)
                                                                                                                              gATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTCCTTCGCCTTTAACGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCATTCTCTTTGGCAAATTTCTCGCGGTACTATTTAcaatt                    >  1:34189/1‑146 (MQ=255)
                                                                                                                                  aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt                <  2:3747782/150‑1 (MQ=255)
                                                                                                                                  aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt                >  1:2887117/1‑150 (MQ=255)
                                                                                                                                    cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTc              >  2:755070/1‑150 (MQ=255)
                                                                                                                                    cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTc              <  2:630268/150‑1 (MQ=255)
                                                                                                                                     gTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg             >  1:4104687/1‑150 (MQ=255)
                                                                                                                                       aCCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGtt           >  1:3312380/1‑150 (MQ=255)
                                                                                                                                        ccTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTa          <  2:3632389/150‑1 (MQ=255)
                                                                                                                                        ccTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGATTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTa          <  1:3123478/150‑1 (MQ=255)
                                                                                                                                         cTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt         <  1:69284/150‑1 (MQ=255)
                                                                                                                                            tGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAg      <  1:110362/150‑1 (MQ=255)
                                                                                                                                             gTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGc     <  2:4757974/150‑1 (MQ=255)
                                                                                                                                               aGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCcg   <  1:1309669/150‑1 (MQ=255)
                                                                                                                                               aGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCcg   >  1:5276164/1‑150 (MQ=255)
                                                                                                                                                gTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCcgc  >  2:3578139/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGC  >  CP009273/3470125‑3470418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑