Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,473,139 G→T 100% I276I (ATC→ATA)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,473,1390GT98.8% 796.4 / 0.2 259I276I (ATC→ATA) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (118/137);  total (121/138)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

CTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA  >  CP009273/3472992‑3473285
                                                                                                                                                   |                                                                                                                                                  
 tGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAtata                                                                                                                                                 <  2:5071478/150‑1 (MQ=255)
   cATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATaaa                                                                                                                                               <  2:818977/150‑1 (MQ=255)
   cATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATaaa                                                                                                                                               >  2:4807803/1‑150 (MQ=255)
   cATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATaaa                                                                                                                                               >  1:2454195/1‑150 (MQ=255)
    aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTCTAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                              >  2:4701199/1‑150 (MQ=255)
    aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                              <  1:1805270/150‑1 (MQ=255)
    aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                              >  2:2308175/1‑150 (MQ=255)
    aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                              >  1:487342/1‑150 (MQ=255)
    aTCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAg                                                                                                                                              >  1:425319/1‑150 (MQ=255)
       cGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCCCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAAc                                                                                                                                           <  2:789778/150‑1 (MQ=255)
        gCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACa                                                                                                                                          <  2:3092107/150‑1 (MQ=255)
          tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaaa                                                                                                                                        <  2:868565/150‑1 (MQ=255)
          tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaaa                                                                                                                                        >  2:3833797/1‑150 (MQ=255)
          tctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACaaa                                                                                                                                        <  1:2297221/150‑1 (MQ=255)
           ctGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAAc                                                                                                                                       <  2:129043/150‑1 (MQ=255)
             gAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTCTAAAGCACCCCGAGGGTGAGTGACATGCCGACAGATATAAAGAACAAACCg                                                                                                                                     >  1:2619235/1‑150 (MQ=255)
             gAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCg                                                                                                                                     <  1:3345257/150‑1 (MQ=255)
             gAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCg                                                                                                                                     >  1:5201535/1‑150 (MQ=255)
                 tACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagca                                                                                                                                 <  2:1900606/150‑1 (MQ=255)
                  acgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagcag                                                                                                                                <  2:738087/150‑1 (MQ=255)
                   cgcacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGagcagc                                                                                                                               >  2:4753108/1‑150 (MQ=255)
                     cacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCaa                                                                                                                             >  2:1578182/1‑150 (MQ=255)
                     cacgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCaa                                                                                                                             <  2:5201535/150‑1 (MQ=255)
                      acgcCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAg                                                                                                                            >  1:2154969/1‑150 (MQ=255)
                          cATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCtt                                                                                                                        >  2:2752030/1‑150 (MQ=255)
                           aTACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCttt                                                                                                                       >  1:1952059/1‑150 (MQ=255)
                              cAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                                                                                                    <  2:3486789/150‑1 (MQ=255)
                               aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                   >  1:2959599/1‑150 (MQ=255)
                               aaTCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                   >  2:4068445/1‑150 (MQ=255)
                                 tCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                                                                                                 <  1:3930696/150‑1 (MQ=255)
                                  cgcgCCAGCAGATACAGCAAGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                                                                                                <  2:4579423/150‑1 (MQ=255)
                                   gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                                                                                               <  2:4517824/150‑1 (MQ=255)
                                   gcgcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGAt                                                                                                               <  1:3169253/150‑1 (MQ=255)
                                    cgcCAGCAGATACAGCACGAGTATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGATGGGATc                                                                                                              <  1:4409779/150‑1 (MQ=255)
                                      ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGa                                                                                                            >  2:2455867/1‑150 (MQ=255)
                                        agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                                                                                                          <  2:2403100/150‑1 (MQ=255)
                                        agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                                                                                                          <  1:5132192/150‑1 (MQ=255)
                                        agcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                                                                                                          <  1:2794825/150‑1 (MQ=255)
                                         gcagATACAGCACGAGAATTTTCACCACCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAg                                                                                                         <  2:3206794/150‑1 (MQ=255)
                                           agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGcc                                                                                                       <  1:5264400/150‑1 (MQ=255)
                                             aTACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGt                                                                                                     >  2:5058820/1‑150 (MQ=255)
                                              tACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                                                                                                    <  2:104115/150‑1 (MQ=255)
                                               aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGttt                                                                                                   >  1:4042539/1‑150 (MQ=255)
                                                  gCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCa                                                                                                <  1:1098811/150‑1 (MQ=255)
                                                   cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAg                                                                                               <  1:4533620/150‑1 (MQ=255)
                                                    aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGt                                                                                              <  2:367322/150‑1 (MQ=255)
                                                      gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTc                                                                                            <  2:4487766/150‑1 (MQ=255)
                                                       agaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCa                                                                                           <  2:636091/150‑1 (MQ=255)
                                                        gaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt                                                                                          <  2:2665185/150‑1 (MQ=255)
                                                         aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg                                                                                         >  2:5319006/1‑150 (MQ=255)
                                                          aTTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg                                                                                        <  2:1102380/150‑1 (MQ=255)
                                                              tCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGat                                                                                    <  2:3073800/150‑1 (MQ=255)
                                                                aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGatat                                                                                  <  2:2858061/150‑1 (MQ=255)
                                                                      accaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACtt                                                                            >  2:2130342/1‑150 (MQ=255)
                                                                        caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                          >  1:1905238/1‑150 (MQ=255)
                                                                        caGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTc                                                                          <  2:1867552/150‑1 (MQ=255)
                                                                          gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCg                                                                        <  2:3907594/150‑1 (MQ=255)
                                                                            acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                      >  2:1645381/1‑150 (MQ=255)
                                                                            acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                      <  1:3930447/150‑1 (MQ=255)
                                                                              aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                    >  1:3559704/1‑150 (MQ=255)
                                                                              aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcag                                                                    >  1:886151/1‑150 (MQ=255)
                                                                                ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCcagca                                                                  >  2:4327399/1‑150 (MQ=255)
                                                                                  aCGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCagcagc                                                                <  1:2057825/150‑1 (MQ=255)
                                                                                    gCTTATCCCTACCCACACCAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCAc                                                              <  1:2411778/150‑1 (MQ=255)
                                                                                    gCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCAc                                                              <  1:3012093/150‑1 (MQ=255)
                                                                                     cTTATCACTACCCACACCAGATGGGTATAAAGCACCCCGAGGTTGAGTGACTTGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACg                                                             <  2:169918/150‑1 (MQ=255)
                                                                                       tATCACTACCCACAACAGATGGGTATAAGGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                           <  1:3894264/150‑1 (MQ=255)
                                                                                        aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGccc                                                          <  1:1565203/150‑1 (MQ=255)
                                                                                             tACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAAt                                                     >  2:1005264/1‑150 (MQ=255)
                                                                                              aCCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATa                                                    >  2:42711/1‑150 (MQ=255)
                                                                                                  acaacaGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATg                                                <  1:1269645/150‑1 (MQ=255)
                                                                                                      caGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTAcc                                            <  2:1443747/150‑1 (MQ=255)
                                                                                                       aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                           >  2:1732699/1‑150 (MQ=255)
                                                                                                       aGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCg                                           <  2:2959599/150‑1 (MQ=255)
                                                                                                        gATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGa                                          >  1:4774542/1‑150 (MQ=255)
                                                                                                             gTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTACCGCCAGCAGCACGCCCGCAATAAAAGTACCGAGCGcc                                     >  2:2491693/1‑150 (MQ=255)
                                                                                                              tataAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCa                                    >  1:3606896/1‑150 (MQ=255)
                                                                                                                taAAGCACCCCGAGTTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCATTTGAAGGGATCGATAGCCGTTTCCAGTTCATTGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                  <  2:4092613/150‑1 (MQ=255)
                                                                                                                taAAGCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATc                                  >  1:1478356/1‑150 (MQ=255)
                                                                                                                 aaaGCACCCCGAGGGTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCg                                 >  1:2362956/1‑150 (MQ=255)
                                                                                                                    gCACCCCGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACa                              <  2:5283611/150‑1 (MQ=255)
                                                                                                                        cccGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGccc                          >  1:1677126/1‑150 (MQ=255)
                                                                                                                         ccGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGcccc                         >  1:3535564/1‑150 (MQ=255)
                                                                                                                          cGAGGTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCa                        <  2:4888822/150‑1 (MQ=255)
                                                                                                                              gTTGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcgc                    >  2:5091546/1‑150 (MQ=255)
                                                                                                                               ttGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCa                   >  1:3448438/1‑150 (MQ=255)
                                                                                                                                tGAGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGACGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCAt                  <  2:1477624/150‑1 (MQ=255)
                                                                                                                                  aGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCCGCGCATcc                >  1:4601553/1‑150 (MQ=255)
                                                                                                                                  aGTGACATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATcc                >  2:3743951/1‑150 (MQ=255)
                                                                                                                                      aCATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaa            >  1:4971909/1‑150 (MQ=255)
                                                                                                                                       cATGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaaa           <  2:4834202/150‑1 (MQ=255)
                                                                                                                                        aTGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAAc          >  1:3256491/1‑150 (MQ=255)
                                                                                                                                         tGCCGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACa         >  1:918140/1‑150 (MQ=255)
                                                                                                                                           ccGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAAt       <  2:5162419/150‑1 (MQ=255)
                                                                                                                                            cGACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATg      >  1:1001559/1‑150 (MQ=255)
                                                                                                                                             gACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGc     >  1:694707/1‑150 (MQ=255)
                                                                                                                                             gACAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGc     >  1:4703001/1‑150 (MQ=255)
                                                                                                                                               cAGATATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCgg   <  1:3784237/150‑1 (MQ=255)
                                                                                                                                                agatATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa  <  1:1474981/150‑1 (MQ=255)
                                                                                                                                                agatATAAAGAACAAACAGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATACACTTTCAGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGa  >  1:183999/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGA  >  CP009273/3472992‑3473285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑