Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,535,370 C→A 100% S535R (AGC→AGA)  feoB → fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,535,3700CA93.4% 761.0 / 3.1 278S535R (AGC→AGA) feoBfused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (3/3);  major base A (164/78);  minor base T (0/30);  total (167/111)
Fisher's exact test for biased strand distribution p-value = 5.65e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535223‑3535517
                                                                                                                                                   |                                                                                                                                                   
 aTGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  <  1:5201871/150‑1 (MQ=255)
 aTGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  <  1:1674024/150‑1 (MQ=255)
  tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  2:2874503/1‑150 (MQ=255)
   ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaa                                                                                                                                                >  1:316999/1‑150 (MQ=255)
     aGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                              >  1:2300293/1‑150 (MQ=255)
     aGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                              <  1:1464819/150‑1 (MQ=255)
     aGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                              >  1:213146/1‑150 (MQ=255)
     aGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCATGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaaa                                                                                                                                              >  2:4203993/1‑150 (MQ=255)
       ttGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtc                                                                                                                                            <  1:2608070/150‑1 (MQ=255)
          ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                         <  1:638264/150‑1 (MQ=255)
          ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtc                                                                                                                                         <  1:4902502/150‑1 (MQ=255)
           cGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcgtcg                                                                                                                                        <  1:3045783/150‑1 (MQ=255)
            ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                       >  1:4524049/1‑150 (MQ=255)
            ggTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                       <  1:4067661/150‑1 (MQ=255)
             gTCTATCATGTACCACTCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGTTCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGAt                                                                                                                                      <  2:2594350/150‑1 (MQ=255)
               cTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATaa                                                                                                                                    <  2:4357636/150‑1 (MQ=255)
                tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAAc                                                                                                                                   >  1:3305504/1‑150 (MQ=255)
                  tCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACAt                                                                                                                                 >  2:324991/1‑150 (MQ=255)
                   cATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                                >  2:2270133/1‑150 (MQ=255)
                      gTACCACACGTTAACAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAc                                                                                                                             <  2:3944102/150‑1 (MQ=255)
                      gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAAc                                                                                                                             <  1:4154676/150‑1 (MQ=255)
                       tACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACg                                                                                                                            <  2:1327363/150‑1 (MQ=255)
                         ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGAc                                                                                                                          <  1:1890998/150‑1 (MQ=255)
                         ccACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGAc                                                                                                                          <  1:1056320/150‑1 (MQ=255)
                          cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACt                                                                                                                         >  2:376299/1‑150 (MQ=255)
                               gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                                    >  2:909225/1‑150 (MQ=255)
                               gTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                                    >  2:515652/1‑150 (MQ=255)
                                  aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                                 >  2:1083512/1‑150 (MQ=255)
                                    aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCTTTCTGCGTGCTGGTAAAGTTATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGATAGGGAAAATCGTCGATAACAGCAACGACTCGGCGCTGGc                                                                                                               <  2:4745757/150‑1 (MQ=255)
                                       ccTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtc                                                                                                            >  2:4976152/1‑150 (MQ=255)
                                          gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                         >  1:2439143/1‑150 (MQ=255)
                                              aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                     >  1:3797233/1‑150 (MQ=255)
                                              aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                     >  1:625267/1‑150 (MQ=255)
                                              aTCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc                                                                                                     >  2:3075833/1‑150 (MQ=255)
                                                ccAGACCTGGCAGCGTCTTAATGGCTTCTTTCTGCGTGCTGTTAATGTGACAATCATCGTCAGAATTTTCCTGAGCGATTTCAACAGCTTCTCGCTGTGTGGTAAAATCGTCGATAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCg                                                                                                   <  1:1240534/150‑1 (MQ=255)
                                                ccAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGATAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg                                                                                                   >  1:4268086/1‑150 (MQ=255)
                                                   gACCTGGCTGCTTCTGAATGGCTTCGTTCTGCGTGCTGTTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGt                                                                                                <  2:4646996/150‑1 (MQ=255)
                                                    aCCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTg                                                                                               >  2:4014240/1‑150 (MQ=255)
                                                     ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCAGCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGa                                                                                              <  2:1188342/150‑1 (MQ=255)
                                                        ggCAGCGTCTGTAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCGTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTGCGTCAGCCGGGTGCTCa                                                                                           >  2:5049247/1‑150 (MQ=255)
                                                        ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGCGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCa                                                                                           <  2:3444547/150‑1 (MQ=255)
                                                        ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCa                                                                                           >  2:3204233/1‑150 (MQ=255)
                                                         gCAGCGTCTTAATGGATTCGTTCTGCGTGCTGTTAATGTTATCATCATCGACAGATTTTTCCTGAGCGATTTCAACAGCTTCTCGCTGATAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAc                                                                                          <  1:4714943/150‑1 (MQ=255)
                                                           aGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                        >  2:4061891/1‑150 (MQ=255)
                                                             cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCg                                                                                      >  1:4885551/1‑150 (MQ=255)
                                                               tcgtaacggCTTCTTTCTGCTTGCTGTTAATTTGTTCTTCATCGTCTGATTTTTCCTGAGCGATTTCAACAGCTTCTCGCTGTGAGGTAAAATGGACGATAACTTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGt                                                                                    <  1:3970633/143‑1 (MQ=255)
                                                               tCTTAATGGCTTCGTTCTGCTTGTTGTTAATGTTATAATAATCTTCATATTTTTCCTGAGCGATTTCAACAGTTTCTCGCTGAGCGTTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGt                                                                                    <  1:1954963/150‑1 (MQ=255)
                                                                  gAATGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCTTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACCTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCtt                                                                                 <  1:1308702/150‑1 (MQ=255)
                                                                   attggCTTCGTTTTGCGTGCTGGTAATGTGTTCATCATCGTCTGCTTTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTc                                                                                <  2:1477341/147‑1 (MQ=255)
                                                                   aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTGTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTc                                                                                <  2:666258/150‑1 (MQ=255)
                                                                            gTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                       >  1:1552115/1‑150 (MQ=255)
                                                                             ttCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTg                                                                      <  2:3055881/150‑1 (MQ=255)
                                                                              tCTGCGTGATGGAAAAGTGATCATCATCGACAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTgg                                                                     <  2:1048948/150‑1 (MQ=255)
                                                                               cTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGc                                                                    >  2:3146190/1‑150 (MQ=255)
                                                                                   gTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  1:3671313/1‑150 (MQ=255)
                                                                                     gCTGTTAATGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                              <  2:5317020/150‑1 (MQ=255)
                                                                                      cTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                             >  1:4973272/1‑150 (MQ=255)
                                                                                      cTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                             >  1:5144175/1‑150 (MQ=255)
                                                                                         ttaatttGATCATCTTCGTCAGCTTTTTCCTTCGGGCTTTCAACTGATTCTCGCTGATTGGTAATATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                          <  1:3435768/144‑1 (MQ=255)
                                                                                         gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                          >  1:1334289/1‑150 (MQ=255)
                                                                                         gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                          >  2:3741103/1‑150 (MQ=255)
                                                                                          tAAAGTGATCATCAACGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATAGGCGTGCAtcgaga                                                         >  1:4349889/1‑145 (MQ=255)
                                                                                           aatgTGATCATCATCGTCAGATTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAt                                                        <  1:3508539/147‑1 (MQ=255)
                                                                                           aaaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAt                                                        >  1:5244926/1‑150 (MQ=255)
                                                                                             aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATaa                                                      >  1:949321/1‑150 (MQ=255)
                                                                                               tGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGATTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                    <  2:450766/150‑1 (MQ=255)
                                                                                               tGATCATCATCGTCAGAATTTTCCTTAGCGATTTAAACTGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                    <  2:1415618/150‑1 (MQ=255)
                                                                                                gATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTg                                                   >  1:111981/1‑150 (MQ=255)
                                                                                                   catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                                >  2:1576455/1‑150 (MQ=255)
                                                                                                      catcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggc                                             >  2:2079055/1‑150 (MQ=255)
                                                                                                       atcGTCTGAATTTTCCTGTGCGATTACAACTGCTTCTCGCTGAGTGGTAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                            <  1:2076211/150‑1 (MQ=255)
                                                                                                         cGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          >  1:4167922/1‑150 (MQ=255)
                                                                                                            cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                       >  1:805154/1‑150 (MQ=255)
                                                                                                              gCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                     >  1:2485590/1‑150 (MQ=255)
                                                                                                              gCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCGGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                     <  2:4174781/150‑1 (MQ=255)
                                                                                                              ccATTTTCCTGAGCGCTTACAACTGCTGCTCGCTGCTAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                     <  2:1906494/149‑1 (MQ=255)
                                                                                                               cATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAGGAAGATAACTGGCAGGCAACGGTTgg                                    >  2:585051/1‑150 (MQ=255)
                                                                                                                aTTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                   >  2:4858592/1‑150 (MQ=255)
                                                                                                                 ttttCCTGCGGGTTTACAACCGGTTCTCGCTGTGTGGTAAATTCGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                  <  1:2874516/150‑1 (MQ=255)
                                                                                                                 ttttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                  >  2:3575628/1‑150 (MQ=255)
                                                                                                                    tCCTGAGCGCTTTCAACAGCTTCTCGCTGATAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                               <  2:245651/150‑1 (MQ=255)
                                                                                                                    tCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                               >  1:2023991/1‑150 (MQ=255)
                                                                                                                      cTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                             >  2:333693/1‑150 (MQ=255)
                                                                                                                         aGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACa                          <  2:317142/150‑1 (MQ=255)
                                                                                                                          gcgcTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAg                         <  2:2884073/150‑1 (MQ=255)
                                                                                                                            gcTTACAACCGCTTCTCGCTGAGCGGTAAAATCGTCTAAAACAACCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                       <  1:3740589/150‑1 (MQ=255)
                                                                                                                                 cAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAt                  >  1:40838/1‑150 (MQ=255)
                                                                                                                                 cAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCAAATTGGCGTTCATGAAGATAACTGGAAGGCAACGGTTGGCCTGTTTACAGGTGCCAt                  >  2:1806333/1‑150 (MQ=255)
                                                                                                                                     aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg              >  1:3193967/1‑150 (MQ=255)
                                                                                                                                     aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg              >  1:69589/1‑150 (MQ=255)
                                                                                                                                     aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg              >  1:4155980/1‑150 (MQ=255)
                                                                                                                                       cTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa            >  2:3090363/1‑150 (MQ=255)
                                                                                                                                          ctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga         >  2:2535073/1‑150 (MQ=255)
                                                                                                                                           tcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa        <  2:1251007/150‑1 (MQ=255)
                                                                                                                                             gCTGAGTGGAAAAATCGTCTATAACACCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt      <  1:5004720/150‑1 (MQ=255)
                                                                                                                                              cTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCACTTGGCGTGCATGAAGATAACTGGCCGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg     >  2:2484861/1‑150 (MQ=255)
                                                                                                                                              cTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg     >  2:3223845/1‑150 (MQ=255)
                                                                                                                                               tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCGGTTTACAGGTGCCATGGCGAAAGAAGTgg    >  1:1447277/1‑150 (MQ=255)
                                                                                                                                                gagaGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt   >  2:803238/1‑150 (MQ=255)
                                                                                                                                                 aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTa  >  2:1373885/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535223‑3535517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑