BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,538,583	C→A	29.7%	R115S (CGC→AGC)	gntX →	DNA catabolic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,538,583	0	C	A	29.7%	676.0 / 242.0	341	R115S (CGC→AGC)	gntX	DNA catabolic protein
Reads supporting (aligned to +/- strand): ref base C (142/97); new base A (49/52); total (191/150)
Fisher's exact test for biased strand distribution p-value = 7.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

TCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAGC  >  CP009273/3538434‑3538730

 cACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCg                                                                                                                                                    >  2:2196405/1‑150 (MQ=255)

   cGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCa                                                                                                                                                  >  1:1698416/1‑150 (MQ=255)

     gTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATc                                                                                                                                                <  1:2275293/150‑1 (MQ=255)

       tGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGt                                                                                                                                              <  1:1621366/150‑1 (MQ=255)

          cGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAg                                                                                                                                           >  1:939792/1‑150 (MQ=255)

          cGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAg                                                                                                                                           >  2:921679/1‑150 (MQ=255)

          cGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAg                                                                                                                                           <  2:168583/150‑1 (MQ=255)

          cGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAg                                                                                                                                           <  1:2207997/150‑1 (MQ=255)

           gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGc                                                                                                                                          >  2:5166211/1‑150 (MQ=255)

           gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGc                                                                                                                                          >  2:4701423/1‑150 (MQ=255)

           gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGc                                                                                                                                          >  2:2544223/1‑150 (MQ=255)

            aCTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCg                                                                                                                                         >  2:3037034/1‑150 (MQ=255)

              tATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGtt                                                                                                                                       <  2:55526/150‑1 (MQ=255)

              tATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGtt                                                                                                                                       >  2:4617733/1‑150 (MQ=255)

                tGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTcc                                                                                                                                     <  1:3268053/150‑1 (MQ=255)

                  cgccgcCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGt                                                                                                                                   >  1:2816258/1‑150 (MQ=255)

                   gccgccGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGtt                                                                                                                                  >  2:4359678/1‑150 (MQ=255)

                   gccgccGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGtt                                                                                                                                  <  2:2337682/150‑1 (MQ=255)

                      gccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATg                                                                                                                               <  1:993278/150‑1 (MQ=255)

                       ccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGATACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATgg                                                                                                                              >  2:1269078/1‑150 (MQ=255)

                        cgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTCTTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGc                                                                                                                             >  2:2898991/1‑150 (MQ=255)

                        cgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGc                                                                                                                             >  1:4877570/1‑150 (MQ=255)

                        cgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGc                                                                                                                             >  1:3357727/1‑150 (MQ=255)

                        cgTTAAGTCCGCCTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGc                                                                                                                             >  2:2587607/1‑150 (MQ=255)

                            aaGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAgcg                                                                                                                         <  1:4973917/150‑1 (MQ=255)

                             aGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAgcgg                                                                                                                        >  2:1042918/1‑150 (MQ=255)

                              gTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAgcggc                                                                                                                       >  2:3356966/1‑150 (MQ=255)

                                  gCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCa                                                                                                                   >  2:5082571/1‑150 (MQ=255)

                                    ttATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACt                                                                                                                 >  2:3281644/1‑150 (MQ=255)

                                     tATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTg                                                                                                                <  1:2960913/150‑1 (MQ=255)

                                        ccaccaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCg                                                                                                             >  2:3561200/1‑150 (MQ=255)

                                          accaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTc                                                                                                           >  1:3910445/1‑150 (MQ=255)

                                           ccaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCg                                                                                                          >  1:2906872/1‑150 (MQ=255)

                                           ccaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCg                                                                                                          <  2:4279852/150‑1 (MQ=255)

                                              gCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCgggg                                                                                                       >  1:3759323/1‑150 (MQ=255)

                                               cTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGa                                                                                                      >  2:4682865/1‑150 (MQ=255)

                                                ttAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGAt                                                                                                     <  2:372894/150‑1 (MQ=255)

                                                 tAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGAtt                                                                                                    <  1:4364255/150‑1 (MQ=255)

                                                  aaaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGAttt                                                                                                   <  2:1853295/150‑1 (MQ=255)

                                                   aaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTa                                                                                                  <  1:1790206/150‑1 (MQ=255)

                                                    aTTTGCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTaa                                                                                                 <  2:2443409/150‑1 (MQ=255)

                                                     ttttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAAt                                                                                                <  1:1055312/150‑1 (MQ=255)

                                                       ttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCa                                                                                              <  1:3748358/150‑1 (MQ=255)

                                                           cGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGc                                                                                          >  1:3478561/1‑150 (MQ=255)

                                                            ggCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCg                                                                                         >  2:1732366/1‑150 (MQ=255)

                                                              cgcAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGAt                                                                                       <  1:4237573/150‑1 (MQ=255)

                                                               gcAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGAtt                                                                                      <  2:4942189/150‑1 (MQ=255)

                                                                 aGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTg                                                                                    >  1:1280580/1‑150 (MQ=255)

                                                                 aGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTg                                                                                    >  1:2426890/1‑150 (MQ=255)

                                                                 aGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTg                                                                                    >  2:4092430/1‑150 (MQ=255)

                                                                  gTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGc                                                                                   >  2:3918292/1‑150 (MQ=255)

                                                                    gAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCtg                                                                                 >  2:5296264/1‑150 (MQ=255)

                                                                      aaTCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCtgtg                                                                               >  2:329777/1‑150 (MQ=255)

                                                                       aTCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTgtgt                                                                              <  1:776054/150‑1 (MQ=255)

                                                                       aTCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTgtgt                                                                              >  1:72329/1‑150 (MQ=255)

                                                                       aTCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTgtgt                                                                              <  1:4464548/150‑1 (MQ=255)

                                                                        tCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTc                                                                             <  2:4561257/150‑1 (MQ=255)

                                                                         cGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCa                                                                            >  2:2396251/1‑150 (MQ=255)

                                                                             aGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCg                                                                        >  2:1550311/1‑150 (MQ=255)

                                                                                gcCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTa                                                                     <  2:2756895/150‑1 (MQ=255)

                                                                                gcCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTa                                                                     >  1:4012795/1‑150 (MQ=255)

                                                                                   cTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCa                                                                  >  1:4792909/1‑150 (MQ=255)

                                                                                    tGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCAc                                                                 <  2:802290/150‑1 (MQ=255)

                                                                                     gTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACg                                                                <  1:4083101/150‑1 (MQ=255)

                                                                                     gTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACg                                                                >  1:1550548/1‑150 (MQ=255)

                                                                                         cGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTgg                                                            >  1:2070635/1‑150 (MQ=255)

                                                                                          gTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGt                                                           >  2:4539164/1‑150 (MQ=255)

                                                                                          gTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGt                                                           >  2:2935927/1‑150 (MQ=255)

                                                                                           tCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGtt                                                          <  2:3663507/150‑1 (MQ=255)

                                                                                            cTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTg                                                         <  2:1367826/150‑1 (MQ=255)

                                                                                              gTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCa                                                       >  1:2747871/1‑150 (MQ=255)

                                                                                                  cTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGc                                                   >  1:5078595/1‑150 (MQ=255)

                                                                                                  cTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGc                                                   >  1:501885/1‑150 (MQ=255)

                                                                                                   tATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGcc                                                  >  2:2685713/1‑150 (MQ=255)

                                                                                                   tATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGcc                                                  >  1:1670626/1‑150 (MQ=255)

                                                                                                       ggAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATg                                              <  2:5213244/150‑1 (MQ=255)

                                                                                                          aGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGa                                           >  1:1091592/1‑150 (MQ=255)

                                                                                                          aGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGa                                           >  1:5233293/1‑150 (MQ=255)

                                                                                                           gTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGAt                                          >  1:4267589/1‑150 (MQ=255)

                                                                                                             cTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAg                                        <  1:5134122/150‑1 (MQ=255)

                                                                                                             cTTACACGCTCGTCGCACCACCGGGGTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGGTGCACGGCCACGGGGATAg                                        >  1:4939852/1‑150 (MQ=255)

                                                                                                              ttACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGCTAGc                                       >  2:4400881/1‑150 (MQ=255)

                                                                                                                       cgtcgCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTc                              >  1:2674506/1‑150 (MQ=255)

                                                                                                                        gtcgCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTca                             <  1:1332938/150‑1 (MQ=255)

                                                                                                                        gtcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTca                             >  2:2083610/1‑150 (MQ=255)

                                                                                                                           gCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCacac                          <  1:255846/150‑1 (MQ=255)

                                                                                                                           gCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCacac                          <  1:2008183/150‑1 (MQ=255)

                                                                                                                              ccaccGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTa                       <  2:4799464/150‑1 (MQ=255)

                                                                                                                                    ggTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACggg                 <  1:4696877/150‑1 (MQ=255)

                                                                                                                                    ggTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACggg                 >  1:3665814/1‑150 (MQ=255)

                                                                                                                                      ttGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGcc               <  2:5041868/150‑1 (MQ=255)

                                                                                                                                         cAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACt            <  1:3997903/150‑1 (MQ=255)

                                                                                                                                         cAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACt            <  1:4617733/150‑1 (MQ=255)

                                                                                                                                          aaTTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTg           <  1:5166211/150‑1 (MQ=255)

                                                                                                                                           aTTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGc          <  1:1439668/150‑1 (MQ=255)

                                                                                                                                            ttGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCg         <  2:4764563/150‑1 (MQ=255)

                                                                                                                                             tGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGa        >  2:1193385/1‑150 (MQ=255)

                                                                                                                                                 ggATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCCAGGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCa    >  1:238702/1‑150 (MQ=255)

                                                                                                                                                 ggATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCa    <  1:4533004/150‑1 (MQ=255)

                                                                                                                                                  gATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAg   >  2:3172351/1‑150 (MQ=255)

TCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAGC  >  CP009273/3538434‑3538730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`