Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,582,807 G→T 100% P194Q (CCG→CAG)  ugpE ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,582,8070GT98.7% 1179.9 / 3.2 365P194Q (CCG→CAG) ugpEglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base T (222/137);  total (227/138)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 365 total aligned reads displayed.

CCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCG  >  CP009273/3582660‑3582953
                                                                                                                                                   |                                                                                                                                                  
ccTTCTCCTGTAGCTATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTACAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGa                                                                                                                                                  <  1:3949513/150‑1 (MQ=255)
 cTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaa                                                                                                                                                 <  2:527927/150‑1 (MQ=255)
 cTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaa                                                                                                                                                 >  2:39598/1‑150 (MQ=255)
    cGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaaa                                                                                                                                              >  1:4455699/1‑150 (MQ=255)
    cGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaaa                                                                                                                                              >  1:3673849/1‑150 (MQ=255)
        tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGAt                                                                                                                                          >  2:2995078/1‑150 (MQ=255)
           aGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTc                                                                                                                                       >  2:884824/1‑150 (MQ=255)
              gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCa                                                                                                                                    >  1:5172371/1‑150 (MQ=255)
              gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCa                                                                                                                                    >  1:4511054/1‑150 (MQ=255)
                    cccTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaa                                                                                                                              <  1:4991198/150‑1 (MQ=255)
                     ccTTTGATCCCGGCCACGGTGGTGCCGAGATCCACATCGTTAAGAATCAACAACGGCCACAAAGACAGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTTGTTTTGGAGATCTGTAAATCGATGTCGCAAaagaag                                                                                                                             <  2:1021479/150‑1 (MQ=255)
                      cTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgc                                                                                                                            >  2:761347/1‑150 (MQ=255)
                      cTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgc                                                                                                                            <  1:2645605/150‑1 (MQ=255)
                        ttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCACAAGAAgcgc                                                                                                                          >  2:1195112/1‑150 (MQ=255)
                         tGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCa                                                                                                                         >  1:1622821/1‑150 (MQ=255)
                          gATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                        <  2:4437845/150‑1 (MQ=255)
                           aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAtt                                                                                                                       <  1:3022333/150‑1 (MQ=255)
                           aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAtt                                                                                                                       <  1:2063175/150‑1 (MQ=255)
                              ccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGc                                                                                                                    >  2:1581526/1‑150 (MQ=255)
                               cTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                   >  2:2773131/1‑150 (MQ=255)
                                 gCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGAt                                                                                                                 <  2:5326131/150‑1 (MQ=255)
                                  ccACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATg                                                                                                                <  2:4651919/150‑1 (MQ=255)
                                   cACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATgc                                                                                                               <  2:1083623/150‑1 (MQ=255)
                                   cACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATgc                                                                                                               <  2:3079085/150‑1 (MQ=255)
                                      ggtggtGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGcc                                                                                                            >  1:671954/1‑150 (MQ=255)
                                       gtggtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCg                                                                                                           >  1:4702494/1‑150 (MQ=255)
                                          gtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCg                                                                                                        >  2:2288032/1‑150 (MQ=255)
                                          gtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCg                                                                                                        <  1:2723954/150‑1 (MQ=255)
                                           tgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGa                                                                                                       >  1:3114459/1‑150 (MQ=255)
                                             ccGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATc                                                                                                     <  2:4354875/150‑1 (MQ=255)
                                              cGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATcc                                                                                                    >  2:1765557/1‑150 (MQ=255)
                                              cGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATcc                                                                                                    <  2:2130603/150‑1 (MQ=255)
                                              cGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATcc                                                                                                    >  1:1378348/1‑150 (MQ=255)
                                               gagaTCCACATCGGTAATAATCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcg                                                                                                   <  2:4467493/150‑1 (MQ=255)
                                                  aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcgc                                                                                                >  2:3384174/1‑150 (MQ=255)
                                                   tCCCCATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcgcg                                                                                               <  1:2250750/150‑1 (MQ=255)
                                                       caTCGGTAATAATCACCCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                           <  1:1019360/150‑1 (MQ=255)
                                                       caTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCtt                                                                                           <  2:2668689/150‑1 (MQ=255)
                                                        aTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTc                                                                                          >  1:4341206/1‑150 (MQ=255)
                                                         tCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTcc                                                                                         <  2:5077074/150‑1 (MQ=255)
                                                           ggTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccac                                                                                       >  1:2012969/1‑150 (MQ=255)
                                                             taataaTCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccacca                                                                                     >  1:2283439/1‑150 (MQ=255)
                                                              aataatCAACAACGGCCACAAAGACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                    <  2:5184142/150‑1 (MQ=255)
                                                               ataatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                   >  2:3734022/1‑150 (MQ=255)
                                                               ataatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGc                                                                                   >  2:5020997/1‑150 (MQ=255)
                                                                tactCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACCGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCt                                                                                  <  1:3309218/147‑1 (MQ=255)
                                                                taatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCt                                                                                  <  2:382984/150‑1 (MQ=255)
                                                                 aatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTc                                                                                 >  1:5225496/1‑150 (MQ=255)
                                                                     aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                             >  1:1096004/1‑150 (MQ=255)
                                                                     aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                             >  1:563914/1‑150 (MQ=255)
                                                                     aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGATGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                             >  2:1524612/1‑150 (MQ=255)
                                                                      acaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCg                                                                            <  1:243793/150‑1 (MQ=255)
                                                                           ggCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGc                                                                       >  2:1303491/1‑150 (MQ=255)
                                                                            gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCg                                                                      <  2:825100/150‑1 (MQ=255)
                                                                             ccACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                     >  1:969655/1‑150 (MQ=255)
                                                                              cacaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAATCGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTc                                                                    >  2:1938585/1‑150 (MQ=255)
                                                                              cacaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTc                                                                    <  1:3555439/150‑1 (MQ=255)
                                                                               acaAATCCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCa                                                                   <  2:635027/150‑1 (MQ=255)
                                                                                caAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCAt                                                                  >  2:2033268/1‑150 (MQ=255)
                                                                                 aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                                 >  2:1697191/1‑150 (MQ=255)
                                                                                 aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                                 >  2:198163/1‑150 (MQ=255)
                                                                                 aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                                 >  1:1908502/1‑150 (MQ=255)
                                                                                  aaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaa                                                                >  1:1120893/1‑150 (MQ=255)
                                                                                   aTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                               >  1:127365/1‑150 (MQ=255)
                                                                                     aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGa                                                             >  2:1253548/1‑150 (MQ=255)
                                                                                        gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACt                                                          >  2:2862602/1‑150 (MQ=255)
                                                                                          ttCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTgg                                                        >  1:1503800/1‑150 (MQ=255)
                                                                                                ccGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAAc                                                  <  1:3235625/150‑1 (MQ=255)
                                                                                                  gTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                                >  1:4184836/1‑150 (MQ=255)
                                                                                                   tAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAgg                                               <  2:3704028/150‑1 (MQ=255)
                                                                                                      aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaaa                                            >  1:492091/1‑150 (MQ=255)
                                                                                                       tAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAg                                           <  1:4621209/150‑1 (MQ=255)
                                                                                                        aaaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                          >  2:4142701/1‑150 (MQ=255)
                                                                                                        aaaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                          >  2:3625511/1‑150 (MQ=255)
                                                                                                         aaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                         >  2:4718855/1‑150 (MQ=255)
                                                                                                         aaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                         >  1:481361/1‑150 (MQ=255)
                                                                                                         aaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                         >  2:4323969/1‑150 (MQ=255)
                                                                                                          aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                        >  1:2549565/1‑150 (MQ=255)
                                                                                                           ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGc                                       >  1:535279/1‑150 (MQ=255)
                                                                                                                  acaAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGcc                                >  1:4078911/1‑150 (MQ=255)
                                                                                                                    aaaCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                              >  2:4028774/1‑150 (MQ=255)
                                                                                                                    aaaCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCACGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                              >  2:3177244/1‑150 (MQ=255)
                                                                                                                           gccgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATc                       <  2:1807119/150‑1 (MQ=255)
                                                                                                                            ccgccAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCa                      >  2:3317581/1‑150 (MQ=255)
                                                                                                                                cAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCgg                  >  1:2903620/1‑150 (MQ=255)
                                                                                                                                cAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCgg                  >  1:3458402/1‑150 (MQ=255)
                                                                                                                                 aGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGc                 >  2:1455311/1‑150 (MQ=255)
                                                                                                                                  gATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCa                >  2:483507/1‑150 (MQ=255)
                                                                                                                                  gATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCa                <  1:1189082/150‑1 (MQ=255)
                                                                                                                                  gATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCa                <  2:3490601/150‑1 (MQ=255)
                                                                                                                                      aGTTTTGGAGAGCTGAAAAACGATTTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt            >  2:3874901/1‑150 (MQ=255)
                                                                                                                                      aGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt            >  1:5275381/1‑150 (MQ=255)
                                                                                                                                      aGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt            >  2:4572570/1‑150 (MQ=255)
                                                                                                                                      aGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt            >  2:1997479/1‑150 (MQ=255)
                                                                                                                                       gTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGtt           >  1:610950/1‑150 (MQ=255)
                                                                                                                                          ttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTaaa        >  1:4563418/1‑150 (MQ=255)
                                                                                                                                           tGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAAc       <  1:3806148/150‑1 (MQ=255)
                                                                                                                                           tGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAAc       <  1:884824/150‑1 (MQ=255)
                                                                                                                                              agagCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCAGATCGGCTTCCACCAGCTCATCCGCCAGCGTCATAAAGAACTGGCGGAACAGGAAAGAAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg    >  2:4229273/1‑150 (MQ=255)
                                                                                                                                                agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCg  >  1:3351655/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGGCG  >  CP009273/3582660‑3582953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑