Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,604,608 C→A 28.1% A162A (GCG→GCT)  yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,604,6080CA28.1% 555.9 / 177.6 267A162A (GCG→GCT) yhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (92/100);  new base A (36/39);  total (128/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 293 total aligned reads displayed.

GCAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGGCT  >  CP009273/3604460‑3604757
                                                                                                                                                    |                                                                                                                                                     
gCAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACccatcgccatcg                                                                                                                                                      >  1:834909/1‑150 (MQ=255)
gCAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACccatcgccatcg                                                                                                                                                      <  2:673850/150‑1 (MQ=255)
     ggTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCg                                                                                                                                                 <  2:3013138/150‑1 (MQ=255)
     ggTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCg                                                                                                                                                 >  1:138185/1‑150 (MQ=255)
     ggTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCg                                                                                                                                                 >  1:4267060/1‑150 (MQ=255)
        ttGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTaa                                                                                                                                              >  1:1075904/1‑150 (MQ=255)
           ccTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTg                                                                                                                                           >  2:4406526/1‑150 (MQ=255)
           ccTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTg                                                                                                                                           >  1:383546/1‑150 (MQ=255)
           ccTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTg                                                                                                                                           >  2:5169226/1‑150 (MQ=255)
            cTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGa                                                                                                                                          >  2:3834820/1‑150 (MQ=255)
                aCTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAAt                                                                                                                                      >  2:2616697/1‑150 (MQ=255)
                aCTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAAt                                                                                                                                      <  2:4302912/150‑1 (MQ=255)
                   ggCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGcc                                                                                                                                   <  2:495995/150‑1 (MQ=255)
                     cTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGt                                                                                                                                 >  2:1962194/1‑150 (MQ=255)
                        ttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTcc                                                                                                                              <  2:1661719/150‑1 (MQ=255)
                        ttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTcc                                                                                                                              >  1:5028616/1‑150 (MQ=255)
                            cGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGa                                                                                                                          >  1:397794/1‑150 (MQ=255)
                            cGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGa                                                                                                                          >  1:1667475/1‑150 (MQ=255)
                             gTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGaa                                                                                                                         >  1:472839/1‑150 (MQ=255)
                               cGGACGCGGGATCGCCAACAAACTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAAt                                                                                                                       <  2:1796840/150‑1 (MQ=255)
                                ggACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATc                                                                                                                      >  1:3227231/1‑150 (MQ=255)
                                 gACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCa                                                                                                                     >  2:2154803/1‑150 (MQ=255)
                                   cgcgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcc                                                                                                                   >  1:1428513/1‑150 (MQ=255)
                                   cgcgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcc                                                                                                                   <  2:5319425/150‑1 (MQ=255)
                                    gcgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAccc                                                                                                                  >  1:262746/1‑150 (MQ=255)
                                      gggATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgc                                                                                                                >  1:553287/1‑150 (MQ=255)
                                      gggATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgc                                                                                                                >  2:1308879/1‑150 (MQ=255)
                                       ggATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgcc                                                                                                               >  2:1545134/1‑150 (MQ=255)
                                         aTCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgcccg                                                                                                             >  2:3578478/1‑150 (MQ=255)
                                         aTCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgcccg                                                                                                             <  2:2742591/150‑1 (MQ=255)
                                          tCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGa                                                                                                            <  1:4540809/150‑1 (MQ=255)
                                           cGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATTAAACTCGACGCCTAACGGCGCACCCATCGCCATCGCCCCTTAAGTGACAATGCCGTTCCACGATATCACCCGCCCGat                                                                                                           <  2:606264/150‑1 (MQ=255)
                                              caacaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATg                                                                                                        >  1:3750387/1‑150 (MQ=255)
                                               aacaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGc                                                                                                       >  1:4949761/1‑150 (MQ=255)
                                               aacaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGc                                                                                                       <  1:1007305/150‑1 (MQ=255)
                                                  aaaaTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGc                                                                                                    <  2:1386588/150‑1 (MQ=255)
                                                     aTGGCCACCAGCGCCACGCCCATAATGATTCACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAg                                                                                                 <  1:3184054/150‑1 (MQ=255)
                                                     aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAg                                                                                                 <  2:5255355/150‑1 (MQ=255)
                                                      tGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGc                                                                                                >  1:2427718/1‑150 (MQ=255)
                                                        gCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCa                                                                                              <  2:3256992/150‑1 (MQ=255)
                                                         ccaccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCaa                                                                                             >  1:3537314/1‑150 (MQ=255)
                                                         ccaccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCaa                                                                                             <  1:2831619/150‑1 (MQ=255)
                                                             caGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCAc                                                                                         <  1:5053642/150‑1 (MQ=255)
                                                                 gcCACTCCCATAATGATTAACGATAACGCCTGCAAGCCGCCCCAGTGATAAAACACGAAGCCTAACTGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCACGATATGCTGCGAGCCAACCActgat                                                                                     <  2:3655091/150‑5 (MQ=255)
                                                                 gcCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCa                                                                                     >  1:3721919/1‑150 (MQ=255)
                                                                      gCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGcc                                                                                >  2:1670017/1‑150 (MQ=255)
                                                                         cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCa                                                                             >  1:2791032/1‑150 (MQ=255)
                                                                         cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCa                                                                             <  2:819447/150‑1 (MQ=255)
                                                                          aTAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCAt                                                                            >  1:21710/1‑150 (MQ=255)
                                                                              tGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAggg                                                                        <  1:2526360/150‑1 (MQ=255)
                                                                                aTTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTc                                                                      >  1:1981067/1‑150 (MQ=255)
                                                                                   ttcgctaacgcCTGCAAGCCGCGCGATTGATAAATCACGACGCCTAACGGCGCACACATAGCCTTCGCCCCGTAAGTGACAATGCCGTTCCTCGATTTCACCCGGCCGATATGCAGCGAGCCAACCCCGCCTACGCCCCATAGGGTCGAt                                                                   <  2:1096622/148‑1 (MQ=255)
                                                                                   aacgctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGAt                                                                   <  2:4579189/150‑1 (MQ=255)
                                                                                     cgctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATcc                                                                 <  1:3064983/150‑1 (MQ=255)
                                                                                         aacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGtt                                                             <  1:3632431/150‑1 (MQ=255)
                                                                                           cgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTcc                                                           <  2:4393195/150‑1 (MQ=255)
                                                                                            gcCTTCAAGCCTCACCAGTGATAAAACACGACGCCTATGGGCTCACAGTTCGCCTTCGCCCCGTAAGTGACTATGACGTTCCACGAAATCTCCCTCCCGATTTGCAGAGTTCCATGCACTCCAACGCCCCATAGGGTCGATGCCGTTCCg                                                          <  2:2886224/150‑1 (MQ=255)
                                                                                            gcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCg                                                          <  1:3422901/150‑1 (MQ=255)
                                                                                               tGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCa                                                       >  1:120961/1‑150 (MQ=255)
                                                                                               tGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCa                                                       >  1:1156633/1‑150 (MQ=255)
                                                                                               tGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCa                                                       >  2:1040579/1‑150 (MQ=255)
                                                                                                gCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaa                                                      <  2:159806/150‑1 (MQ=255)
                                                                                                gCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaa                                                      <  2:2326975/150‑1 (MQ=255)
                                                                                                   aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                   >  1:4832400/1‑150 (MQ=255)
                                                                                                   aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                   >  1:4500906/1‑150 (MQ=255)
                                                                                                   aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                   <  1:5169226/150‑1 (MQ=255)
                                                                                                    gccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACt                                                  <  1:617744/150‑1 (MQ=255)
                                                                                                      cgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACttt                                                <  1:2236526/150‑1 (MQ=255)
                                                                                                      cgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACttt                                                <  2:5203510/150‑1 (MQ=255)
                                                                                                      cgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACttt                                                <  1:2199325/150‑1 (MQ=255)
                                                                                                         cccAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGcc                                             >  1:218853/1‑150 (MQ=255)
                                                                                                            aGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCaa                                          <  1:4083050/150‑1 (MQ=255)
                                                                                                            aGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCaa                                          <  1:2770848/150‑1 (MQ=255)
                                                                                                            aGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCaa                                          >  2:148799/1‑150 (MQ=255)
                                                                                                            aGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCaa                                          >  2:4483555/1‑150 (MQ=255)
                                                                                                                aTAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATccc                                      >  2:3925808/1‑150 (MQ=255)
                                                                                                                aTAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATccc                                      >  2:3736561/1‑150 (MQ=255)
                                                                                                                  aaaaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCaa                                    >  1:773886/1‑150 (MQ=255)
                                                                                                                    aaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGGTCCGGCAAAACTTTGCCCAATCCCAAgg                                  >  1:950052/1‑150 (MQ=255)
                                                                                                                     acacGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGa                                 >  1:3671317/1‑150 (MQ=255)
                                                                                                                      cacGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGAt                                <  1:2846161/150‑1 (MQ=255)
                                                                                                                      cacGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGAGATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGAt                                >  2:488674/1‑150 (MQ=255)
                                                                                                                        cgacgCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGa                              >  2:1783203/1‑150 (MQ=255)
                                                                                                                         gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCTCGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGAc                             <  1:1317104/150‑1 (MQ=255)
                                                                                                                         gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGAc                             <  2:3103480/150‑1 (MQ=255)
                                                                                                                           cgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGAcgc                           >  1:652662/1‑150 (MQ=255)
                                                                                                                                 aCGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCa                     >  2:4216459/1‑150 (MQ=255)
                                                                                                                                   ggCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACAACGCAAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCatg                   <  2:4546198/150‑3 (MQ=255)
                                                                                                                                      gcACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCaa                >  2:3560817/1‑150 (MQ=255)
                                                                                                                                        aCCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAg              >  1:5307577/1‑150 (MQ=255)
                                                                                                                                        aCCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAg              >  1:4755921/1‑150 (MQ=255)
                                                                                                                                          ccatcgccatagCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGta            >  1:5231992/1‑150 (MQ=255)
                                                                                                                                          ccatcgccatagCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGta            <  2:4765161/150‑1 (MQ=255)
                                                                                                                                            atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat          <  2:3120295/150‑1 (MQ=255)
                                                                                                                                            atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat          >  1:1240499/1‑150 (MQ=255)
                                                                                                                                            atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat          <  1:183392/150‑1 (MQ=255)
                                                                                                                                               gccatcgccCCGTAAGTGACACTGCCGTTCCACGACATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAAc       <  2:5017729/150‑1 (MQ=255)
                                                                                                                                                 catcgacCCTTAAGTGACAATGCCGTTCAACGAAATCACCCGCACGATATGCAGCGAGCAAACCACGCAAACGACCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACTCGCCCAAGGCAAAGTAATAACat     >  2:3997198/1‑149 (MQ=255)
                                                                                                                                                  atcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAgg    <  2:837397/150‑1 (MQ=255)
                                                                                                                                                    cgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGGCt  >  1:2152758/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGGCT  >  CP009273/3604460‑3604757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑