Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,630,577 T→A 13.1% Q65L (CAG→CTG)  yhiN ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,630,5770TA13.1% 691.6 / 80.5 259Q65L (CAG→CTG) yhiNputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (109/116);  new base A (19/15);  total (128/131)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 264 total aligned reads displayed.

CCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATA  >  CP009273/3630428‑3630726
                                                                                                                                                     |                                                                                                                                                     
ccaGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCt                                                                                                                                                       <  1:596003/150‑1 (MQ=255)
    cATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAATTGCGGAGTCTGGCt                                                                                                                                                   >  2:3076095/1‑150 (MQ=255)
    cATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCt                                                                                                                                                   <  1:1542052/150‑1 (MQ=255)
    cATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCt                                                                                                                                                   <  1:2397567/150‑1 (MQ=255)
          gACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATa                                                                                                                                             >  1:41109/1‑150 (MQ=255)
            cAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAg                                                                                                                                           <  2:3918079/150‑1 (MQ=255)
            cAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAg                                                                                                                                           <  2:4494733/150‑1 (MQ=255)
             aaTCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAgc                                                                                                                                          >  2:5206710/1‑150 (MQ=255)
                ctgctgCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGcc                                                                                                                                       <  2:288544/150‑1 (MQ=255)
                 tgctgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCt                                                                                                                                      <  2:417865/150‑1 (MQ=255)
                 tgctgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCt                                                                                                                                      <  2:3238677/150‑1 (MQ=255)
                    tgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGt                                                                                                                                   <  2:4829758/150‑1 (MQ=255)
                    tgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGt                                                                                                                                   <  1:3611213/150‑1 (MQ=255)
                      cgcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCATTGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTc                                                                                                                                 <  1:3790958/150‑1 (MQ=255)
                      cgcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTc                                                                                                                                 <  1:1951320/150‑1 (MQ=255)
                      cgcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTc                                                                                                                                 <  2:3806123/150‑1 (MQ=255)
                             tCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATaa                                                                                                                          >  2:3781415/1‑150 (MQ=255)
                              cATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATaaa                                                                                                                         >  1:2461241/1‑150 (MQ=255)
                               aTCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAg                                                                                                                        <  1:1278820/150‑1 (MQ=255)
                               aTCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAg                                                                                                                        <  1:870110/150‑1 (MQ=255)
                                 cGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGt                                                                                                                      <  2:2229164/150‑1 (MQ=255)
                                  gCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGtt                                                                                                                     >  2:3118111/1‑150 (MQ=255)
                                   cAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTg                                                                                                                    >  1:2880430/1‑150 (MQ=255)
                                    agaagaGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTgg                                                                                                                   >  2:358507/1‑150 (MQ=255)
                                         agTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAg                                                                                                              <  1:2003156/150‑1 (MQ=255)
                                         agTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAg                                                                                                              <  1:4487169/150‑1 (MQ=255)
                                           ttGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGtt                                                                                                            <  1:1892008/150‑1 (MQ=255)
                                           ttGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGtt                                                                                                            <  2:649601/150‑1 (MQ=255)
                                            ggCCCTCACGTTGTCTCGTGCCTGGCGATGCCGTGTTTTTTTACCAGATCAATTAAATCCCACTGGGTAAAACGTGAGAGTGCAGACTTACAAAATTGCGTTTTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTg                                                                                                           <  1:5011916/149‑1 (MQ=255)
                                              cccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCa                                                                                                         >  1:5042211/1‑150 (MQ=255)
                                               ccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAg                                                                                                        >  2:1126694/1‑150 (MQ=255)
                                               ccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAg                                                                                                        >  1:824495/1‑150 (MQ=255)
                                               ccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAg                                                                                                        >  1:4881834/1‑150 (MQ=255)
                                               ccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAg                                                                                                        <  2:4567752/150‑1 (MQ=255)
                                               ccTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAg                                                                                                        >  2:4699661/1‑150 (MQ=255)
                                                  aaCGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAgcgc                                                                                                     >  2:2635453/1‑150 (MQ=255)
                                                   aCGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGcc                                                                                                    <  1:3037428/150‑1 (MQ=255)
                                                    cGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGccc                                                                                                   >  1:683077/1‑150 (MQ=255)
                                                     gTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCa                                                                                                  >  2:2684192/1‑150 (MQ=255)
                                                        ttCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCg                                                                                               >  1:5100129/1‑150 (MQ=255)
                                                              tGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGAc                                                                                         >  2:4395252/1‑150 (MQ=255)
                                                                 cAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATa                                                                                      >  1:3196736/1‑150 (MQ=255)
                                                                 cAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACAGAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATa                                                                                      >  1:3420984/1‑150 (MQ=255)
                                                                  aGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAAGGTGCGAGTGCAGACTTACAAAAATGCGGTTTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATaa                                                                                     <  1:98453/150‑1 (MQ=255)
                                                                  aGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATaa                                                                                     >  1:4176903/1‑150 (MQ=255)
                                                                      gATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGAt                                                                                 >  1:3386598/1‑150 (MQ=255)
                                                                       aTGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGAtt                                                                                <  1:1788048/150‑1 (MQ=255)
                                                                       aTGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGAtt                                                                                <  2:737122/150‑1 (MQ=255)
                                                                        tGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGAttt                                                                               <  1:851859/150‑1 (MQ=255)
                                                                         gCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGAtttt                                                                              >  2:1791071/1‑150 (MQ=255)
                                                                           cGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTgc                                                                            >  1:3026754/1‑150 (MQ=255)
                                                                           cGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTgc                                                                            <  2:1630513/150‑1 (MQ=255)
                                                                            gtgtTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTgcg                                                                           >  1:2218162/1‑150 (MQ=255)
                                                                              gtTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGcc                                                                         <  1:1157016/150‑1 (MQ=255)
                                                                                  aTTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTgg                                                                     <  1:4524174/150‑1 (MQ=255)
                                                                                    tGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGtt                                                                   <  2:591405/150‑1 (MQ=255)
                                                                                    tGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAATCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGtt                                                                   >  2:84622/1‑150 (MQ=255)
                                                                                      aCCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGtttt                                                                 <  2:4352810/150‑1 (MQ=255)
                                                                                       ccAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGttttt                                                                <  1:3744608/150‑1 (MQ=255)
                                                                                        cAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGtttttt                                                               <  2:4978882/150‑1 (MQ=255)
                                                                                         aGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTa                                                              <  1:4797140/150‑1 (MQ=255)
                                                                                           aTCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTAcc                                                            >  1:1039237/1‑150 (MQ=255)
                                                                                           aTCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTAcc                                                            <  2:949778/150‑1 (MQ=255)
                                                                                           aTCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTAcc                                                            >  1:3236069/1‑150 (MQ=255)
                                                                                             cAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCAt                                                          >  2:5144916/1‑150 (MQ=255)
                                                                                               aTGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTa                                                        <  1:1003075/150‑1 (MQ=255)
                                                                                                tGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTAt                                                       <  1:554355/150‑1 (MQ=255)
                                                                                                 gAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATc                                                      <  2:228378/150‑1 (MQ=255)
                                                                                                 gAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATc                                                      <  2:2514421/150‑1 (MQ=255)
                                                                                                  aaaTCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCg                                                     >  2:2716107/1‑150 (MQ=255)
                                                                                                   aaTCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGa                                                    <  2:2633225/150‑1 (MQ=255)
                                                                                                   aaTCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGa                                                    >  1:1160603/1‑150 (MQ=255)
                                                                                                      cccACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATca                                                 >  2:3714391/1‑150 (MQ=255)
                                                                                                       ccACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATcag                                                <  2:977158/150‑1 (MQ=255)
                                                                                                       ccACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATcag                                                <  2:3850239/150‑1 (MQ=255)
                                                                                                       ccACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATcag                                                >  1:2727493/1‑150 (MQ=255)
                                                                                                            gggTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGaa                                           >  2:3046468/1‑150 (MQ=255)
                                                                                                              gTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAAcc                                         <  2:4607681/150‑1 (MQ=255)
                                                                                                                aaaaCGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCg                                       >  1:2829434/1‑150 (MQ=255)
                                                                                                                aaaaCGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCg                                       <  2:3034399/150‑1 (MQ=255)
                                                                                                                    cGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGt                                   >  1:2662337/1‑150 (MQ=255)
                                                                                                                    cGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGt                                   >  1:3969454/1‑150 (MQ=255)
                                                                                                                       gCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTcct                                >  2:1844620/1‑150 (MQ=255)
                                                                                                                       gCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTcct                                <  1:4833032/150‑1 (MQ=255)
                                                                                                                          aGTGCAGACTTACAAAAATGCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTcctgcc                             >  1:777999/1‑150 (MQ=255)
                                                                                                                            tGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTcctgcctg                           <  2:2902103/150‑1 (MQ=255)
                                                                                                                                gACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCt                       <  2:497668/150‑1 (MQ=255)
                                                                                                                                  cTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGc                     >  2:4056502/1‑150 (MQ=255)
                                                                                                                                    tACCAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCa                   <  2:2826585/150‑1 (MQ=255)
                                                                                                                                    tACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCa                   <  1:3898947/150‑1 (MQ=255)
                                                                                                                                             gCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAATGTTGCAGCGCCCACCGCCAGACATTAGGATTTTGCGCCCTTGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaac          <  2:4733283/150‑1 (MQ=255)
                                                                                                                                             gCGGATTCAGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaac          <  2:3402001/150‑1 (MQ=255)
                                                                                                                                              cGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaaca         >  1:2591223/1‑150 (MQ=255)
                                                                                                                                               ggATTCTGGCTCAGATAAGTGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaacag        >  1:4274172/1‑150 (MQ=255)
                                                                                                                                               ggATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaacag        <  2:1784777/150‑1 (MQ=255)
                                                                                                                                               ggATTCTGGCTCAGATAAGCGCCTGGTTCGACAGAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTTCCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaacag        >  2:3176708/1‑150 (MQ=255)
                                                                                                                                                gATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGcagaacaga       >  2:205411/1‑150 (MQ=255)
                                                                                                                                                   tCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAgaacagaaca    <  2:1667632/150‑1 (MQ=255)
                                                                                                                                                     tGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATa  >  2:541283/1‑150 (MQ=255)
                                                                                                                                                     tGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATa  >  1:4675539/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCCTGGTTCGACATAAAGGTTGGTAAAGTTGCAGCGCCCACCGCCAGACATAAGGATTTTGCGCCCTGGTTTTTTACCATTATCGATCAGCAGAACCCGGCGTCCTGCCTGACCTGCCAGCGCAGAACAGAACATA  >  CP009273/3630428‑3630726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑