Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,637,274 C→A 13.4% M423I (ATG→ATT)  prlC ← oligopeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,637,2740CA13.4% 858.6 / 66.7 269M423I (ATG→ATT) prlColigopeptidase A
Reads supporting (aligned to +/- strand):  ref base C (108/124);  new base A (21/16);  total (129/140)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

TGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGATGCCGTA  >  CP009273/3637125‑3637423
                                                                                                                                                     |                                                                                                                                                     
    cGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCatccatcc                                                                                                                                                   <  1:4317706/150‑1 (MQ=255)
     gAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCAtccatcca                                                                                                                                                  >  1:2513876/1‑150 (MQ=255)
      aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCAc                                                                                                                                                 <  2:4946396/150‑1 (MQ=255)
      aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGGTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGCCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCAc                                                                                                                                                 >  1:1398926/1‑150 (MQ=255)
       aCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACg                                                                                                                                                >  2:3197154/1‑150 (MQ=255)
          cGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                                             <  1:3342441/150‑1 (MQ=255)
          cGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                                             <  1:3018275/150‑1 (MQ=255)
              gAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                                                         >  2:2017872/1‑150 (MQ=255)
              gAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                                                         >  1:5167498/1‑150 (MQ=255)
                   gggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                                                    >  2:5931/1‑150 (MQ=255)
                   gggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                                                    >  1:361872/1‑150 (MQ=255)
                   gggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                                                    >  1:4751579/1‑150 (MQ=255)
                    ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCtt                                                                                                                                   <  2:3798777/150‑1 (MQ=255)
                      tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGt                                                                                                                                 <  2:2601876/150‑1 (MQ=255)
                       gATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtt                                                                                                                                >  2:2817564/1‑150 (MQ=255)
                       gATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGtt                                                                                                                                <  2:5006085/150‑1 (MQ=255)
                        aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                                               >  2:3756358/1‑150 (MQ=255)
                             ttcttcGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                                                          <  1:215911/150‑1 (MQ=255)
                              tcgtcgAGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACgg                                                                                                                         <  2:3065336/150‑1 (MQ=255)
                               cgtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCGTGTTTTCAAggg                                                                                                                        >  1:1356582/1‑150 (MQ=255)
                                gtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGc                                                                                                                       <  2:4219882/150‑1 (MQ=255)
                                gtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGc                                                                                                                       <  1:2945161/150‑1 (MQ=255)
                                   gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                                                    >  1:26895/1‑150 (MQ=255)
                                    tgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATAc                                                                                                                   <  2:1324403/150‑1 (MQ=255)
                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                                               <  2:1718095/150‑1 (MQ=255)
                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                                               <  2:3734706/150‑1 (MQ=255)
                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                                               >  2:222963/1‑150 (MQ=255)
                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                                               <  1:1007006/150‑1 (MQ=255)
                                        tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                                               <  1:1239340/150‑1 (MQ=255)
                                         gaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATc                                                                                                              <  2:1530192/150‑1 (MQ=255)
                                            cAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAg                                                                                                           <  2:463912/150‑1 (MQ=255)
                                             aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAgg                                                                                                          <  2:2190627/150‑1 (MQ=255)
                                             aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAgg                                                                                                          <  2:3546602/150‑1 (MQ=255)
                                              gcgcGGGTTTACCATTTACCGGGCGGTTGAAGTTACATGTCAAATACGCGACCGGTTTTTGCAAAGATCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGt                                                                                                         <  1:1716748/150‑1 (MQ=255)
                                                     ttttCCATTTACCGGGCGGTTGAAGTTACAAGTCAATGTCGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACTCAGTCATCAATCCCCGCCCCGCCGCGCTTGTTTTCTCGGGCATACAGATCGAGGTAGAAGCt                                                                                                  <  1:5250587/150‑1 (MQ=255)
                                                       tACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTAc                                                                                                <  2:1653696/150‑1 (MQ=255)
                                                        aCCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCGTACAGATCGAGGTAGAAGCTAcc                                                                                               <  1:851262/150‑1 (MQ=255)
                                                          cATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgc                                                                                             >  2:478286/1‑150 (MQ=255)
                                                          cATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgc                                                                                             <  2:2335491/150‑1 (MQ=255)
                                                           aTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                                            >  1:2550846/1‑150 (MQ=255)
                                                           aTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                                            >  1:4220022/1‑150 (MQ=255)
                                                                ccGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGtt                                                                                       <  1:3452056/150‑1 (MQ=255)
                                                                 cGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTc                                                                                      >  1:2841593/1‑150 (MQ=255)
                                                                 cGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTc                                                                                      >  1:834371/1‑150 (MQ=255)
                                                                 cGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTc                                                                                      <  1:3825773/150‑1 (MQ=255)
                                                                  gggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCg                                                                                     <  2:1823475/150‑1 (MQ=255)
                                                                   ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGt                                                                                    >  2:3880385/1‑150 (MQ=255)
                                                                      ggTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                                                 >  1:1816526/1‑150 (MQ=255)
                                                                      ggTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                                                 <  2:35927/150‑1 (MQ=255)
                                                                        ttGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAttt                                                                               <  1:1127890/150‑1 (MQ=255)
                                                                               tACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCAt                                                                        <  2:2951497/150‑1 (MQ=255)
                                                                               tACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCAt                                                                        >  2:470861/1‑150 (MQ=255)
                                                                                aaaaGTAAAATACGAGACAGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGAATACAGATAGAGGTAGAAGCTAACGCGCAGTTCGTTATTTTCTTCATa                                                                       >  2:888929/3‑150 (MQ=255)
                                                                                 cAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATAc                                                                      <  1:657791/150‑1 (MQ=255)
                                                                                    gTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGt                                                                   <  2:1178703/150‑1 (MQ=255)
                                                                                     tCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGtt                                                                  <  1:3671866/150‑1 (MQ=255)
                                                                                      cAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTc                                                                 >  1:4068915/1‑150 (MQ=255)
                                                                                         aTACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaa                                                              >  2:1128634/1‑150 (MQ=255)
                                                                                             gcgACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGaaa                                                          >  1:53070/1‑150 (MQ=255)
                                                                                               gACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACg                                                        >  1:3149181/1‑150 (MQ=255)
                                                                                                aCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGt                                                       >  1:2822450/1‑150 (MQ=255)
                                                                                                   ggTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACa                                                    >  2:1283813/1‑150 (MQ=255)
                                                                                                    gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACAt                                                   <  1:3688912/150‑1 (MQ=255)
                                                                                                       tttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCg                                                <  1:4118274/150‑1 (MQ=255)
                                                                                                           cAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCCTCCATCCACGCCCCGCCGCGCTTGTTCTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGAAGTTCGTTATTTTCTTCATACAGTTCGAAGAAACGTACATCCGGATg                                            >  2:266901/1‑150 (MQ=255)
                                                                                                           cAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATg                                            <  1:4931519/150‑1 (MQ=255)
                                                                                                            aaaGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGc                                           >  2:3030648/1‑150 (MQ=255)
                                                                                                             aaGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCAAAGAAACGTACATCCGGATGcc                                          <  2:3457031/150‑1 (MQ=255)
                                                                                                             aaGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCAAAGAAACGTACATCCGGATGcc                                          <  2:1398926/150‑1 (MQ=255)
                                                                                                              aGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCa                                         >  1:3771069/1‑150 (MQ=255)
                                                                                                                 aCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGAc                                      <  2:2642463/150‑1 (MQ=255)
                                                                                                                   cATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATATAGTTCGAAGAAACGTACATCCGGATGCCAGACAt                                    >  1:2300908/1‑150 (MQ=255)
                                                                                                                   cATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACAt                                    <  2:2513876/150‑1 (MQ=255)
                                                                                                                   cATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACAt                                    >  1:1160053/1‑150 (MQ=255)
                                                                                                                   cATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACAt                                    <  1:4179815/150‑1 (MQ=255)
                                                                                                                    aTCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATc                                   >  2:4330222/1‑150 (MQ=255)
                                                                                                                     tCAGCTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGACGCTACCGCGCAGTACGTAATTTTCGTCATACAGTTCGAAGAAACTTACATCCGGAAGCCAGACATCa                                  >  1:198336/1‑150 (MQ=255)
                                                                                                                         cTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAt                              >  2:4370600/1‑150 (MQ=255)
                                                                                                                         cTTTACGCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAt                              >  2:4493346/1‑150 (MQ=255)
                                                                                                                          tttACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATc                             <  1:1056377/150‑1 (MQ=255)
                                                                                                                               gCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTAc                        >  1:964113/1‑150 (MQ=255)
                                                                                                                               gCATCTGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTAc                        <  2:4735304/150‑1 (MQ=255)
                                                                                                                                cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACg                       >  1:3936477/1‑150 (MQ=255)
                                                                                                                                cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGATCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACg                       >  2:3407797/1‑150 (MQ=255)
                                                                                                                                 aTCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGc                      >  2:5305432/1‑150 (MQ=255)
                                                                                                                                    tGGCCTACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGctct                   >  1:1867988/1‑150 (MQ=255)
                                                                                                                                      gCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGAACAACCGGATGCCAGACATCAACATCTTTACTCTCttt                 >  1:4373368/1‑150 (MQ=255)
                                                                                                                                         tACGCAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAACCGTACATCAGGATGCCAGACATCAACATCTTTACGATCTTTGGc              >  2:50653/1‑150 (MQ=255)
                                                                                                                                             cAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTg          >  1:348369/1‑150 (MQ=255)
                                                                                                                                             cAGTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTg          <  2:964113/150‑1 (MQ=255)
                                                                                                                                              aGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGa         >  2:5156285/1‑150 (MQ=255)
                                                                                                                                              aGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGa         >  2:764738/1‑150 (MQ=255)
                                                                                                                                               gTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGAt        <  1:5066236/150‑1 (MQ=255)
                                                                                                                                               gTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGAt        <  2:2159164/150‑1 (MQ=255)
                                                                                                                                               gTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGAt        <  1:1604089/150‑1 (MQ=255)
                                                                                                                                               gTCATCAATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGAt        >  2:3226762/1‑150 (MQ=255)
                                                                                                                                                tCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCCACATCTTTACGCTCTTTAGCGGTGATg       <  1:4469006/150‑1 (MQ=255)
                                                                                                                                                  atccatccACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGATGcc     <  2:4154484/150‑1 (MQ=255)
                                                                                                                                                  atcaatccACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGATGcc     <  1:699991/150‑1 (MQ=255)
                                                                                                                                                     catccaCGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGATGCCGTa  >  1:4567757/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACATCTTTACGCTCTTTAGCGGTGATGCCGTA  >  CP009273/3637125‑3637423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑