Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,639,239 G→T 100% P165P (CCG→CCT)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,639,2390GT97.6% 922.8 / 10.4 295P165P (CCG→CCT) rlmJ23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (140/148);  total (144/151)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 297 total aligned reads displayed.

GACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGAC  >  CP009273/3639092‑3639385
                                                                                                                                                   |                                                                                                                                                  
gACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACcctcc                                                                                                                                                  <  1:3097759/150‑1 (MQ=255)
    gAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTat                                                                                                                                              <  2:5037743/150‑1 (MQ=255)
     aaCTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatg                                                                                                                                             <  1:1192691/150‑1 (MQ=255)
       cTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaa                                                                                                                                           >  1:2286769/1‑150 (MQ=255)
         gCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTatgaaat                                                                                                                                         <  1:5150215/150‑1 (MQ=255)
               gAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAAc                                                                                                                                   >  2:1955689/1‑150 (MQ=255)
                   gATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGAc                                                                                                                               >  2:4103554/1‑150 (MQ=255)
                    aTTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACt                                                                                                                              <  2:404786/150‑1 (MQ=255)
                     ttACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTa                                                                                                                             >  1:4373264/1‑150 (MQ=255)
                       aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                           <  1:3312214/150‑1 (MQ=255)
                       aCCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATc                                                                                                                           <  2:601383/150‑1 (MQ=255)
                        cccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACACTCCGTATGAAATGAAAACTGACTATCa                                                                                                                          >  1:3595201/1‑150 (MQ=255)
                         ccGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCaa                                                                                                                         >  2:5294304/1‑150 (MQ=255)
                          cGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAg                                                                                                                        <  2:3529053/150‑1 (MQ=255)
                           gttgttGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGc                                                                                                                       >  2:4063201/1‑150 (MQ=255)
                           gttgttGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGc                                                                                                                       >  1:4862008/1‑150 (MQ=255)
                           gttgttGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGc                                                                                                                       <  1:4799235/150‑1 (MQ=255)
                             tgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCgg                                                                                                                     >  1:291990/1‑150 (MQ=255)
                             tgttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCgg                                                                                                                     >  1:455277/1‑150 (MQ=255)
                              gttgCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGGGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggt                                                                                                                    >  2:4826714/1‑150 (MQ=255)
                                 gCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCggtggt                                                                                                                 >  1:1325078/1‑150 (MQ=255)
                                  cGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTc                                                                                                                >  1:3929195/1‑150 (MQ=255)
                                      cTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCg                                                                                                            <  2:1531333/150‑1 (MQ=255)
                                        gAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCggg                                                                                                          >  1:1079805/1‑150 (MQ=255)
                                          aTTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGAt                                                                                                        >  2:2180415/1‑150 (MQ=255)
                                           tttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATa                                                                                                       >  1:180328/1‑150 (MQ=255)
                                           tttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGGTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATa                                                                                                       <  2:634181/150‑1 (MQ=255)
                                            ttCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAACACTGACTATCAAGCGGTGGTCTGCGGGATAg                                                                                                      >  1:1561093/1‑150 (MQ=255)
                                              cAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCa                                                                                                    <  1:3888640/150‑1 (MQ=255)
                                                gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                                  >  1:151986/1‑150 (MQ=255)
                                                gAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGa                                                                                                  <  1:2502815/150‑1 (MQ=255)
                                                  aaGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGGTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAg                                                                                                <  2:3304870/150‑1 (MQ=255)
                                                     aTAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGtt                                                                                             >  2:3042840/1‑150 (MQ=255)
                                                     aTAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGtt                                                                                             <  2:1995543/150‑1 (MQ=255)
                                                     aTAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGtt                                                                                             <  1:193810/150‑1 (MQ=255)
                                                       aGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTAc                                                                                           <  1:1474407/150‑1 (MQ=255)
                                                       aGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTAc                                                                                           <  2:1847213/150‑1 (MQ=255)
                                                       aGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTAc                                                                                           >  1:3545662/1‑150 (MQ=255)
                                                              cgcgTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGtt                                                                                    <  1:4006292/150‑1 (MQ=255)
                                                                 gtgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCg                                                                                 <  2:3269459/150‑1 (MQ=255)
                                                                 gtgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCg                                                                                 >  2:3951374/1‑150 (MQ=255)
                                                                  tgtCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGc                                                                                >  2:5136813/1‑150 (MQ=255)
                                                                      gAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                            >  2:2816640/1‑150 (MQ=255)
                                                                      gAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACt                                                                            >  1:5201314/1‑150 (MQ=255)
                                                                       aaaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTg                                                                           >  2:3439804/1‑150 (MQ=255)
                                                                        aaaaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTgg                                                                          <  1:2210175/150‑1 (MQ=255)
                                                                          aaGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTa                                                                        >  1:3236062/1‑150 (MQ=255)
                                                                           aGCCGACGGTTACCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTAt                                                                       >  1:2737140/1‑150 (MQ=255)
                                                                              cGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGGGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTGTCGCCACTGGTATTTa                                                                    >  2:3701552/1‑150 (MQ=255)
                                                                               gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                   <  1:5286049/150‑1 (MQ=255)
                                                                               gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                   <  2:708457/150‑1 (MQ=255)
                                                                               gACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTAc                                                                   <  1:216754/150‑1 (MQ=255)
                                                                                aCGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                  >  2:4323015/1‑150 (MQ=255)
                                                                                aCGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                  >  2:1316886/1‑150 (MQ=255)
                                                                                  ggTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                >  2:4720352/1‑150 (MQ=255)
                                                                                       ccAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtgtg                                                           >  2:3774286/1‑150 (MQ=255)
                                                                                          gcagcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTa                                                        <  2:4894898/150‑1 (MQ=255)
                                                                                            agcTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTGCGCCACTGGTATTTACGCACTGTGGTATc                                                      >  1:4983720/1‑150 (MQ=255)
                                                                                              cTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCg                                                    <  1:2336997/150‑1 (MQ=255)
                                                                                                 aaGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCCAGCGGTGGTCAGAGGGATAGCAGAAGGTTACAAACGTTTCGCAACTGGTATTAACGCACTGTCGTATCCtgtg                                                 >  1:3397691/1‑150 (MQ=255)
                                                                                                  aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                >  2:4277907/1‑150 (MQ=255)
                                                                                                  aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                <  2:4442794/150‑1 (MQ=255)
                                                                                                  aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                <  2:3406100/150‑1 (MQ=255)
                                                                                                  aGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTGATCGACCCTCCGTATGAAATGAAAACTGACTCTCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCggtgg                                                <  2:3899220/150‑1 (MQ=255)
                                                                                                     ccAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgc                                             <  1:3181796/150‑1 (MQ=255)
                                                                                                      cAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgct                                            <  1:3708603/150‑1 (MQ=255)
                                                                                                       aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATTAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                           <  1:4516315/150‑1 (MQ=255)
                                                                                                       aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                           >  1:2033136/1‑150 (MQ=255)
                                                                                                       aaaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctg                                           <  2:1877126/150‑1 (MQ=255)
                                                                                                        aaCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGtgctgc                                          >  1:543781/1‑150 (MQ=255)
                                                                                                         aCTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCg                                         <  1:4397891/150‑1 (MQ=255)
                                                                                                            gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCa                                      <  2:4985148/150‑1 (MQ=255)
                                                                                                            gccgccGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGGTACAAACGTTTCGCCACGGGTATTTACGCACTGTGGTATCCGGGGGTGCTGCGTCa                                      >  2:1403711/1‑150 (MQ=255)
                                                                                                                  ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                >  2:635314/1‑150 (MQ=255)
                                                                                                                  ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                >  1:3425908/1‑150 (MQ=255)
                                                                                                                  ggTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAt                                >  2:2066108/1‑150 (MQ=255)
                                                                                                                   gTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                               <  2:3861364/150‑1 (MQ=255)
                                                                                                                   gTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                               <  2:1762671/150‑1 (MQ=255)
                                                                                                                   gTTTCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAAtt                               >  2:4676556/1‑150 (MQ=255)
                                                                                                                     ttCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTaa                             <  1:977023/150‑1 (MQ=255)
                                                                                                                      tCCCGCCGTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAg                            >  1:833338/1‑150 (MQ=255)
                                                                                                                        ccgccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAgcg                          <  2:5229891/150‑1 (MQ=255)
                                                                                                                          gccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                        >  1:750494/1‑150 (MQ=255)
                                                                                                                           ccgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCACATTAAGCGCAt                       >  2:1581627/1‑150 (MQ=255)
                                                                                                                            cgTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATg                      <  2:74429/150‑1 (MQ=255)
                                                                                                                             gTGGTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGa                     <  1:2839288/150‑1 (MQ=255)
                                                                                                                               ggTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                   >  2:1906969/1‑150 (MQ=255)
                                                                                                                               ggTTTAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                   <  1:5235967/150‑1 (MQ=255)
                                                                                                                                 tttAATCCTTATCGACCCTCCGTATGAAATGACAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                 <  2:897536/150‑1 (MQ=255)
                                                                                                                                  ttAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCAc                <  1:1989804/150‑1 (MQ=255)
                                                                                                                                   tAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg               >  2:1102439/1‑150 (MQ=255)
                                                                                                                                   tAATCCTTATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACg               <  2:3100669/150‑1 (MQ=255)
                                                                                                                                     aTCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGAt             <  2:2716356/150‑1 (MQ=255)
                                                                                                                                          tATCGTCCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCCAATTAAGCGCATGATCCACGTTCTGGa        >  1:4465844/1‑150 (MQ=255)
                                                                                                                                          tATCGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGa        >  1:612437/1‑150 (MQ=255)
                                                                                                                                             cGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc     >  2:3124722/1‑150 (MQ=255)
                                                                                                                                             cGACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGc     >  2:3612440/1‑150 (MQ=255)
                                                                                                                                              gACCCTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg    <  2:1467347/150‑1 (MQ=255)
                                                                                                                                              gACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCg    >  1:1436299/1‑150 (MQ=255)
                                                                                                                                                cccTCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGAc  >  2:2344353/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACCGAACTGCACCCGAGCGATTACCCGTTGTTGCGTTCTGAATTTCAGAAAGATAGCCGTGCGCGTGTCGAAAAAGCCGACGGTTTCCAGCAGCTTAAGGCCAAACTGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGAC  >  CP009273/3639092‑3639385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑