BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,649,460	T→A	60.5%	K65* (AAA→TAA)	hdeB ←	acid‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,649,460	0	T	A	60.5%	292.6 / inf	372	K65* (AAA→TAA)	hdeB	acid‑resistance protein
Reads supporting (aligned to +/- strand): ref base T (86/60); new base A (128/97); total (214/158)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 389 total aligned reads displayed.

TTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACA  >  CP009273/3649311‑3649608

 ttACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTta                                                                                                                                                     <  2:2864196/150‑1 (MQ=255)

    cTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatat                                                                                                                                                  >  2:2602489/1‑150 (MQ=255)

     ttGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatata                                                                                                                                                 <  2:1688752/150‑1 (MQ=255)

      tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATAc                                                                                                                                                <  2:5320286/150‑1 (MQ=255)

       gCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACt                                                                                                                                               <  1:2458405/150‑1 (MQ=255)

         aCCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGt                                                                                                                                             >  2:1445904/1‑150 (MQ=255)

            tCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTttc                                                                                                                                          >  1:577066/1‑150 (MQ=255)

             cATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTttct                                                                                                                                         <  2:693232/150‑1 (MQ=255)

             cATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTttct                                                                                                                                         >  1:214464/1‑150 (MQ=255)

              aTTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTttctt                                                                                                                                        <  1:1972333/150‑1 (MQ=255)

                     cGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACTGTATCGCCACCTTAATATACTGTTTCTTCATgcag                                                                                                                                 >  1:4066825/1‑150 (MQ=255)

                      ggCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATgcagc                                                                                                                                <  1:3734875/150‑1 (MQ=255)

                      ggCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgcagc                                                                                                                                >  1:5129437/1‑150 (MQ=255)

                        cAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGACTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATAGACTGTTTCTTCATGCAGCAt                                                                                                                              >  2:4005004/1‑150 (MQ=255)

                        cAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCAt                                                                                                                              >  1:962459/1‑150 (MQ=255)

                         aaGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATc                                                                                                                             >  2:2362544/1‑150 (MQ=255)

                            tCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccac                                                                                                                          >  1:641373/1‑150 (MQ=255)

                            tCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccac                                                                                                                          >  2:122672/1‑150 (MQ=255)

                            tCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccac                                                                                                                          <  1:1529947/150‑1 (MQ=255)

                             cATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccacc                                                                                                                         >  2:4914224/1‑150 (MQ=255)

                             cATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccacc                                                                                                                         >  1:393681/1‑150 (MQ=255)

                              aTTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccacca                                                                                                                        <  2:3882740/150‑1 (MQ=255)

                              aTTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATccacca                                                                                                                        >  1:13040/1‑150 (MQ=255)

                                 aGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGc                                                                                                                     >  2:4162404/1‑150 (MQ=255)

                                   aTGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCaa                                                                                                                   >  1:3304919/1‑150 (MQ=255)

                                     gCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAAcc                                                                                                                 >  1:3466215/1‑150 (MQ=255)

                                      cTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCg                                                                                                                <  1:2239307/150‑1 (MQ=255)

                                             tttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCAt                                                                                                         <  2:1921817/150‑1 (MQ=255)

                                                 aaGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCt                                                                                                     >  1:801251/1‑150 (MQ=255)

                                                    gTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCtttt                                                                                                  <  1:5219266/150‑1 (MQ=255)

                                                    gTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCtttt                                                                                                  >  1:1998057/1‑150 (MQ=255)

                                                      ataCAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTgg                                                                                                >  2:4068913/1‑150 (MQ=255)

                                                        aCAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGAt                                                                                              >  2:3397796/1‑150 (MQ=255)

                                                          aaaTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTc                                                                                            <  2:1131426/150‑1 (MQ=255)

                                                          aaaTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTc                                                                                            >  1:1189787/1‑150 (MQ=255)

                                                           aaTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCa                                                                                           <  2:13491/150‑1 (MQ=255)

                                                           aaTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCa                                                                                           >  1:3803787/1‑150 (MQ=255)

                                                           aaTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTATAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCa                                                                                           >  2:209028/1‑150 (MQ=255)

                                                            aTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAg                                                                                          <  1:4212439/150‑1 (MQ=255)

                                                             tttttCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGa                                                                                         >  1:4921247/1‑150 (MQ=255)

                                                             attttCTGAGTGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGAATCAACCATTCAACCGGGGTCATTGCTTTTGTATTCAGa                                                                                         >  2:4624923/2‑150 (MQ=255)

                                                               tttCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATc                                                                                       <  2:3148266/150‑1 (MQ=255)

                                                                  cTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAAt                                                                                    >  1:3068826/1‑150 (MQ=255)

                                                                  cTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAAt                                                                                    <  2:2578015/150‑1 (MQ=255)

                                                                   tGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATa                                                                                   >  2:1089005/1‑150 (MQ=255)

                                                                    gCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATaa                                                                                  <  1:2395015/150‑1 (MQ=255)

                                                                    gCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATaa                                                                                  <  1:2918549/150‑1 (MQ=255)

                                                                      gggTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAAt                                                                                <  1:1042385/150‑1 (MQ=255)

                                                                        gTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTc                                                                              >  1:1768625/1‑150 (MQ=255)

                                                                        gTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTc                                                                              <  2:2391683/150‑1 (MQ=255)

                                                                         tttttCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTcc                                                                             <  1:837099/150‑1 (MQ=255)

                                                                         tttttCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTcc                                                                             <  1:4909158/150‑1 (MQ=255)

                                                                            ttCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTgg                                                                          >  2:4824910/1‑150 (MQ=255)

                                                                             tCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGc                                                                         <  1:4172544/150‑1 (MQ=255)

                                                                              cTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCa                                                                        >  1:2645135/1‑150 (MQ=255)

                                                                               ttACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAg                                                                       >  2:366891/1‑150 (MQ=255)

                                                                                tACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAgg                                                                      >  1:1007004/1‑150 (MQ=255)

                                                                                tACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAgg                                                                      <  1:3961464/150‑1 (MQ=255)

                                                                                      aTTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCatat                                                                >  2:1745472/1‑150 (MQ=255)

                                                                                         cGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCtt                                                             >  1:2318213/1‑150 (MQ=255)

                                                                                           aTCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGAGTCAGATCAATAAATTCCTGGCAGGTCATATCTTTa                                                           >  1:4658831/1‑150 (MQ=255)

                                                                                            tCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAg                                                          <  1:2034162/150‑1 (MQ=255)

                                                                                            tCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAg                                                          <  2:796244/150‑1 (MQ=255)

                                                                                             cACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGc                                                         >  2:1772116/1‑150 (MQ=255)

                                                                                             cACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGc                                                         <  2:833063/150‑1 (MQ=255)

                                                                                                   aGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTc                                                   <  1:172162/150‑1 (MQ=255)

                                                                                                      aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                                <  2:3491823/150‑1 (MQ=255)

                                                                                                      aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                                <  2:4328520/150‑1 (MQ=255)

                                                                                                      aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                                >  1:545607/1‑150 (MQ=255)

                                                                                                      aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                                <  1:5015484/150‑1 (MQ=255)

                                                                                                       aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTg                                               >  1:3157687/1‑150 (MQ=255)

                                                                                                          tgagtgagATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCt                                            <  1:471332/150‑1 (MQ=255)

                                                                                                          tgagtgagATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCt                                            >  1:1841674/1‑150 (MQ=255)

                                                                                                          tgagtgagATCGGTTTCATTTAAAGTAACGGGATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCt                                            <  2:5094545/150‑1 (MQ=255)

                                                                                                              tgagATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCa                                        <  2:3308772/150‑1 (MQ=255)

                                                                                                                agaTCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAAc                                      >  1:5137302/1‑150 (MQ=255)

                                                                                                                agaTCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAAc                                      >  2:3052334/1‑150 (MQ=255)

                                                                                                                  aTCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGAGGATTCATTGGCTGCCAACGc                                    >  2:55975/1‑150 (MQ=255)

                                                                                                                    cGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAg                                  >  2:3848168/1‑150 (MQ=255)

                                                                                                                     ggTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                                 >  2:923390/1‑150 (MQ=255)

                                                                                                                      gTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGAt                                >  1:452650/1‑150 (MQ=255)

                                                                                                                          cATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATtgtg                            <  2:214464/150‑1 (MQ=255)

                                                                                                                           aTTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGc                           >  1:724204/1‑150 (MQ=255)

                                                                                                                           aTTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGc                           >  2:2818250/1‑150 (MQ=255)

                                                                                                                           aTTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGc                           >  1:4304743/1‑150 (MQ=255)

                                                                                                                              tAAAGTAACGGTATCGCCACCTTAATATACTGTTTC‑TCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTc                       >  1:4486213/1‑150 (MQ=255)

                                                                                                                                 aGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCAc                     <  1:3119384/150‑1 (MQ=255)

                                                                                                                                   tAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCa                   <  2:1832801/150‑1 (MQ=255)

                                                                                                                                    aaCGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg                  >  2:4440329/1‑150 (MQ=255)

                                                                                                                                    aaCGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg                  >  1:1444219/1‑150 (MQ=255)

                                                                                                                                     aCGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGt                 >  2:298811/1‑150 (MQ=255)

                                                                                                                                         tATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACa             >  1:4839096/1‑150 (MQ=255)

                                                                                                                                         tATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACa             >  2:4211674/1‑150 (MQ=255)

                                                                                                                                             gCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGc         >  1:3344147/1‑150 (MQ=255)

                                                                                                                                              ccACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGcc        <  1:3397796/150‑1 (MQ=255)

                                                                                                                                              ccACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGcc        <  2:1589325/150‑1 (MQ=255)

                                                                                                                                                 ccTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGACAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCt     >  1:3761781/1‑150 (MQ=255)

                                                                                                                                                  cTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTa    >  1:4434995/1‑150 (MQ=255)

                                                                                                                                                  cTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTa    >  1:4991382/1‑150 (MQ=255)

                                                                                                                                                    ttATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACa  <  2:522616/150‑1 (MQ=255)

TTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACA  >  CP009273/3649311‑3649608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`