Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,679,324 C→A 100% M1070I (ATG→ATT)  bcsC ← cellulose synthase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,679,3240CA99.7% 929.7 / ‑4.6 282M1070I (ATG→ATT) bcsCcellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (172/109);  total (172/110)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 293 total aligned reads displayed.

CTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679177‑3679470
                                                                                                                                                   |                                                                                                                                                  
ctgGATATCAATTGCCGTGCCAACAACCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaat                                                                                                                                                  <  2:1455487/150‑1 (MQ=255)
ctgGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaat                                                                                                                                                  <  1:2930968/150‑1 (MQ=255)
  ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCCAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatca                                                                                                                                                <  2:1235837/150‑1 (MQ=255)
  ggATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATtaatca                                                                                                                                                >  1:2145983/1‑150 (MQ=255)
    atatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                              >  1:1199564/1‑150 (MQ=255)
    atatCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGc                                                                                                                                              <  2:2403290/150‑1 (MQ=255)
      atCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCAGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGATTCTTCCGGCCAGTCGGTCGGTATCAGATTAATCAGCgg                                                                                                                                            <  2:5155521/150‑1 (MQ=255)
       tCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGa                                                                                                                                           >  1:4942099/1‑150 (MQ=255)
         aaTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATa                                                                                                                                         <  2:3843629/150‑1 (MQ=255)
          aTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATaa                                                                                                                                        >  1:4425917/1‑150 (MQ=255)
          aTTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATaa                                                                                                                                        >  1:2783456/1‑150 (MQ=255)
            tGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAcg                                                                                                                                      <  2:4237519/150‑1 (MQ=255)
            tGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAcg                                                                                                                                      <  2:111393/150‑1 (MQ=255)
              ccGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAAcgcg                                                                                                                                    >  1:2724921/1‑150 (MQ=255)
                gTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGc                                                                                                                                  >  1:2530130/1‑150 (MQ=255)
                 tGCCAACAAACCAGTTGGAAGTAACACGTCGTTAAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCAGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCa                                                                                                                                 >  1:4720816/1‑150 (MQ=255)
                   ccAACAAACCAGATGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATg                                                                                                                               <  2:2188139/150‑1 (MQ=255)
                     aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                             >  1:46719/1‑150 (MQ=255)
                     aacaaACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGt                                                                                                                             >  1:4879586/1‑150 (MQ=255)
                        aaaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGtttt                                                                                                                          >  2:1976542/1‑150 (MQ=255)
                         aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTg                                                                                                                         <  1:38486/150‑1 (MQ=255)
                         aaCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTg                                                                                                                         >  2:5180491/1‑150 (MQ=255)
                          aCCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTgg                                                                                                                        <  2:1189291/150‑1 (MQ=255)
                           ccAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGt                                                                                                                       <  1:967326/150‑1 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      >  1:4783339/1‑150 (MQ=255)
                            cAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTg                                                                                                                      >  1:745846/1‑150 (MQ=255)
                             aGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGAGTGCCGCGCAGCTTCTTCCGGCCAGTAGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGc                                                                                                                     <  1:3052948/150‑1 (MQ=255)
                               ttGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGt                                                                                                                   >  2:956835/1‑150 (MQ=255)
                                tGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTg                                                                                                                  >  2:1332270/1‑150 (MQ=255)
                                 ggAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTACCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGa                                                                                                                 >  1:3476821/1‑150 (MQ=255)
                                    aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                              >  1:873485/1‑150 (MQ=255)
                                    aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                              >  2:1653334/1‑150 (MQ=255)
                                    aGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATg                                                                                                              <  1:4210329/150‑1 (MQ=255)
                                      tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                            >  2:3491208/1‑150 (MQ=255)
                                      tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                            >  2:5051287/1‑150 (MQ=255)
                                      tAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCg                                                                                                            >  2:539405/1‑150 (MQ=255)
                                        acacGTCGTTCAAGTAAGGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGAc                                                                                                          <  2:2855078/150‑1 (MQ=255)
                                           cgtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCAc                                                                                                       <  1:2595997/150‑1 (MQ=255)
                                            gtcgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACGGCTGCCGCCATCGTGGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACg                                                                                                      <  2:1581360/150‑1 (MQ=255)
                                              cgtTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAg                                                                                                    >  2:445863/1‑150 (MQ=255)
                                                ttCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGcc                                                                                                  <  2:2689369/150‑1 (MQ=255)
                                                ttCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGcc                                                                                                  <  1:1954752/150‑1 (MQ=255)
                                                 tCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCa                                                                                                 <  2:175949/150‑1 (MQ=255)
                                                  cAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCCTCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGAGCAGATTAATCAGCGGATAACGCGGCATGGTGTTGGTGCGTGAATGCGACCACGAGCCAg                                                                                                >  2:467499/1‑150 (MQ=255)
                                                  cAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAg                                                                                                <  2:3661120/150‑1 (MQ=255)
                                                    aGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGAc                                                                                              <  1:2844790/150‑1 (MQ=255)
                                                     gTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACg                                                                                             >  2:5319855/1‑150 (MQ=255)
                                                     gTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACg                                                                                             <  1:3601047/150‑1 (MQ=255)
                                                      tAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGc                                                                                            >  1:612111/1‑150 (MQ=255)
                                                        aTGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc                                                                                          >  2:700631/1‑150 (MQ=255)
                                                        aTGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCAc                                                                                          >  2:314900/1‑150 (MQ=255)
                                                           cccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCa                                                                                       <  1:1050025/150‑1 (MQ=255)
                                                            ccGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAg                                                                                      >  1:1706681/1‑150 (MQ=255)
                                                               cgcCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTc                                                                                   <  2:2724942/150‑1 (MQ=255)
                                                                gcCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTcc                                                                                  >  1:5001095/1‑150 (MQ=255)
                                                                 ccGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTccc                                                                                 <  2:4342880/150‑1 (MQ=255)
                                                                  cGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCa                                                                                <  1:3681760/150‑1 (MQ=255)
                                                                  cGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCa                                                                                <  1:1387643/150‑1 (MQ=255)
                                                                   gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGTGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               >  2:841674/1‑150 (MQ=255)
                                                                   gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCAc                                                                               >  1:3107677/1‑150 (MQ=255)
                                                                    tgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACg                                                                              <  2:3595589/150‑1 (MQ=255)
                                                                      tAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAc                                                                            >  1:3905469/1‑150 (MQ=255)
                                                                       aGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGAcc                                                                           <  2:2807250/150‑1 (MQ=255)
                                                                           gAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                       >  1:84866/1‑150 (MQ=255)
                                                                           gAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                       >  1:4260848/1‑150 (MQ=255)
                                                                           gAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtt                                                                       <  2:4729778/150‑1 (MQ=255)
                                                                            aaGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGttt                                                                      >  1:5051303/1‑150 (MQ=255)
                                                                             aGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGtttt                                                                     <  1:2219540/150‑1 (MQ=255)
                                                                                ccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCg                                                                  >  2:4869507/1‑150 (MQ=255)
                                                                                  tGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTg                                                                >  2:3379700/1‑150 (MQ=255)
                                                                                   gACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgc                                                               <  2:4929888/150‑1 (MQ=255)
                                                                                     ctgctgCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTgcgc                                                             <  2:1074893/150‑1 (MQ=255)
                                                                                      tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCt                                                            >  2:2767907/1‑150 (MQ=255)
                                                                                      tgctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCt                                                            >  2:4068757/1‑150 (MQ=255)
                                                                                       gctgcCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTc                                                           >  2:2150244/1‑150 (MQ=255)
                                                                                             gccATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCca                                                     >  1:4492187/1‑150 (MQ=255)
                                                                                               cATCGTTGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCcaca                                                   >  1:537683/1‑150 (MQ=255)
                                                                                                    ttGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCAcc                                              >  2:2986895/1‑150 (MQ=255)
                                                                                                     tGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCg                                             <  1:3270904/150‑1 (MQ=255)
                                                                                                     tGGATTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCg                                             <  2:376300/150‑1 (MQ=255)
                                                                                                        aTTGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTa                                          >  1:5069063/1‑150 (MQ=255)
                                                                                                         gtGCCGCGCAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTAt                                         <  1:3165778/149‑1 (MQ=255)
                                                                                                              gcgcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCaa                                    <  2:3293526/150‑1 (MQ=255)
                                                                                                               cgcAGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCaaa                                   >  1:5135553/1‑150 (MQ=255)
                                                                                                                  aGCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGa                                >  1:3253899/1‑150 (MQ=255)
                                                                                                                   gCTTCTTCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGAc                               <  2:25211/150‑1 (MQ=255)
                                                                                                                    cttcttCCGGCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACa                              >  1:1902608/1‑150 (MQ=255)
                                                                                                                      tcttcCGGCCAGTCGGTGGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCTCCACATCCCCGTTATGGAAAACGACAgg                            <  2:4576076/150‑1 (MQ=255)
                                                                                                                           cggCCAGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTc                       >  1:3400019/3‑150 (MQ=255)
                                                                                                                                aGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGAGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCg                  >  1:2746763/1‑150 (MQ=255)
                                                                                                                                aGTCGGTCGGGATCAGATTAATCAGCGGATAACGCGGATTGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCg                  <  1:335365/150‑1 (MQ=255)
                                                                                                                                     gtcggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTg             >  1:3986951/1‑150 (MQ=255)
                                                                                                                                       cggGATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgta           >  1:1809304/1‑150 (MQ=255)
                                                                                                                                        gggATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtag          >  1:698783/1‑150 (MQ=255)
                                                                                                                                          gATCAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgtagta        >  1:4610498/1‑150 (MQ=255)
                                                                                                                                             cataTTAATCCGCGGATACCGGGGCTTGTTTTTGGTGCTTGATTGCGACCACGAGCCACACGCACCCAGCACCCACGACCTTTTTTCCGTGCGCCCCCGCCAAATCACCGGTTTGGAAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  1:2368096/147‑1 (MQ=255)
                                                                                                                                             cataTTAATCAGCGTATAAGGCGGCTTGGTTTTGGTGCGTGATTGCGACCACGAGCCAGACGCACCCTGCTCCCACGACCAGTTTGCGGTGCGCTCCCGCCAAATCACCGGTTTGGAAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     <  2:4133086/147‑1 (MQ=255)
                                                                                                                                             cAGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc     >  1:1345208/1‑150 (MQ=255)
                                                                                                                                              aGATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCg    >  1:58252/1‑150 (MQ=255)
                                                                                                                                               gATTAATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGc   >  1:2094716/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTTTGGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCC  >  CP009273/3679177‑3679470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑