Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,698,875 G→T 100% F92L (TTC→TTA)  dppB ← dipeptide/heme transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,698,8750GT99.4% 1035.1 / ‑2.3 321F92L (TTC→TTA) dppBdipeptide/heme transporter
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (149/170);  total (149/172)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 321 total aligned reads displayed.

ATAACCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGAT  >  CP009273/3698728‑3699021
                                                                                                                                                   |                                                                                                                                                  
  aaCCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAAct                                                                                                                                                >  1:5258011/1‑150 (MQ=255)
     cTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCtt                                                                                                                                             >  2:2063319/1‑150 (MQ=255)
         cAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCa                                                                                                                                         <  2:4869552/150‑1 (MQ=255)
             gcCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAAcc                                                                                                                                     <  2:3883592/150‑1 (MQ=255)
             gcCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAAcc                                                                                                                                     >  2:1688735/1‑150 (MQ=255)
             gcCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAAcc                                                                                                                                     >  2:540446/1‑150 (MQ=255)
                  gCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGAt                                                                                                                                <  2:2091858/150‑1 (MQ=255)
                   ccAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATg                                                                                                                               <  1:95674/150‑1 (MQ=255)
                   ccAACCGCTGTGTGATCGAAAATGGAACCGAGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATg                                                                                                                               >  2:4099187/1‑150 (MQ=255)
                    cAACCGCTGTGTGATCGAAAATGGAGCCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGc                                                                                                                              <  2:4648624/150‑1 (MQ=255)
                     aaCCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGACGCGCGGCACTAACTCTTCCCAAACCGGGATGCg                                                                                                                             >  2:3496037/1‑150 (MQ=255)
                      aCCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCgg                                                                                                                            >  1:5059852/1‑150 (MQ=255)
                        cGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCt                                                                                                                          <  1:2263318/150‑1 (MQ=255)
                         gCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCtt                                                                                                                         <  1:3751009/150‑1 (MQ=255)
                          cTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCttt                                                                                                                        >  2:1425767/1‑150 (MQ=255)
                           tgtgtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCtttt                                                                                                                       <  2:69465/150‑1 (MQ=255)
                           tgtgtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCtttt                                                                                                                       >  1:4741974/1‑150 (MQ=255)
                            gtgtgATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTc                                                                                                                      >  1:1737671/1‑150 (MQ=255)
                                gATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTg                                                                                                                  >  2:3909038/1‑150 (MQ=255)
                                  tCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGaa                                                                                                                >  1:1156892/1‑150 (MQ=255)
                                  tCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGaa                                                                                                                <  2:151167/150‑1 (MQ=255)
                                   cGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGaaa                                                                                                               <  2:4544834/150‑1 (MQ=255)
                                    gAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAAt                                                                                                              <  2:115732/150‑1 (MQ=255)
                                    gAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAAt                                                                                                              <  1:4110159/150‑1 (MQ=255)
                                     aaaaTGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATg                                                                                                             >  1:1354096/1‑150 (MQ=255)
                                     aaaaTGGAACCGAGTTTAACCGCAGCCAGCACGCCGACAGGAATACCGACTGCCGTAGCAAAATTCATCGCGCAGACGCCAATTTCCAGCGTGGCCTGGAAGCGCGTCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATg                                                                                                             <  1:3071723/150‑1 (MQ=255)
                                      aaaTGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGc                                                                                                            >  1:1692449/1‑150 (MQ=255)
                                         tGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTa                                                                                                         <  1:4873645/150‑1 (MQ=255)
                                         tGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTa                                                                                                         <  2:299006/150‑1 (MQ=255)
                                              ccGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCg                                                                                                    <  1:1260329/150‑1 (MQ=255)
                                               cgcgTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGc                                                                                                   >  1:4233967/1‑150 (MQ=255)
                                                gcgTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGcc                                                                                                  >  1:2674934/1‑150 (MQ=255)
                                                 cgTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCa                                                                                                 >  2:2767163/1‑150 (MQ=255)
                                                 cgTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCa                                                                                                 >  1:4361839/1‑150 (MQ=255)
                                                     tAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCa                                                                                             >  2:623101/1‑150 (MQ=255)
                                                       aCCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATa                                                                                           <  2:4594229/150‑1 (MQ=255)
                                                         cGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAAc                                                                                         <  2:3540567/150‑1 (MQ=255)
                                                         cGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAAc                                                                                         <  2:1037132/150‑1 (MQ=255)
                                                          gCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACg                                                                                        <  1:4037505/150‑1 (MQ=255)
                                                          gCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACg                                                                                        <  2:1256389/150‑1 (MQ=255)
                                                            agccagcACGCCGATCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcc                                                                                      >  1:2984241/1‑150 (MQ=255)
                                                            agccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcc                                                                                      <  1:448599/150‑1 (MQ=255)
                                                            agccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcc                                                                                      >  2:2622172/1‑150 (MQ=255)
                                                            agccagcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGcc                                                                                      >  1:3503543/1‑150 (MQ=255)
                                                             gccagcACGCCGACCGGAATACCGACTGCCGTAGAAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGccc                                                                                     <  2:1583701/150‑1 (MQ=255)
                                                                agcACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCaa                                                                                  >  1:3939070/1‑150 (MQ=255)
                                                                   aCGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATg                                                                               >  1:748275/1‑150 (MQ=255)
                                                                    cGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGt                                                                              <  2:3821737/150‑1 (MQ=255)
                                                                      ccGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTaa                                                                            >  2:3731498/1‑150 (MQ=255)
                                                                      ccGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTaa                                                                            <  2:1376613/150‑1 (MQ=255)
                                                                       cGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAAt                                                                           >  2:4316332/1‑150 (MQ=255)
                                                                        gACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATg                                                                          <  1:4196182/150‑1 (MQ=255)
                                                                         aCCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATgg                                                                         >  2:2258750/1‑150 (MQ=255)
                                                                         aCCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATgg                                                                         <  2:3847076/150‑1 (MQ=255)
                                                                          ccGGTATAGCGACTGGAGTAGCAAATATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCTTAACGCCCCAATTGTAATGga                                                                        <  2:4681564/150‑1 (MQ=255)
                                                                             gAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGAt                                                                     <  1:3183498/150‑1 (MQ=255)
                                                                               aTACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAc                                                                   <  1:393022/150‑1 (MQ=255)
                                                                                tACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATAct                                                                  >  1:3980130/1‑150 (MQ=255)
                                                                                  ccGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgc                                                                >  1:908534/1‑150 (MQ=255)
                                                                                   cGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATActgct                                                               <  1:1004291/150‑1 (MQ=255)
                                                                                     aCTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACtgctgc                                                             <  2:1645895/150‑1 (MQ=255)
                                                                                       tGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCca                                                           <  2:3173727/150‑1 (MQ=255)
                                                                                        gCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCcac                                                          >  1:3325984/1‑150 (MQ=255)
                                                                                        gCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCcac                                                          >  1:858386/1‑150 (MQ=255)
                                                                                             aGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCgg                                                     >  2:2529436/1‑150 (MQ=255)
                                                                                               caacaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                   <  2:2318890/150‑1 (MQ=255)
                                                                                               cAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                   >  2:5110496/1‑150 (MQ=255)
                                                                                               cAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                   >  1:2170062/1‑150 (MQ=255)
                                                                                               cAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGtt                                                   <  1:2947421/150‑1 (MQ=255)
                                                                                                  aaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTAt                                                <  2:5261086/150‑1 (MQ=255)
                                                                                                  aaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTAt                                                <  1:4937725/150‑1 (MQ=255)
                                                                                                  aaaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTAt                                                <  1:2291688/150‑1 (MQ=255)
                                                                                                   aaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATc                                               >  1:820956/1‑150 (MQ=255)
                                                                                                   aaTCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATc                                               >  2:3336197/1‑150 (MQ=255)
                                                                                                    atcatcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCt                                              <  1:2861322/150‑1 (MQ=255)
                                                                                                     tcatcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTa                                             >  2:2483610/1‑150 (MQ=255)
                                                                                                       atcGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAg                                           >  2:2871949/1‑150 (MQ=255)
                                                                                                              aGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGtt                                    >  1:707256/1‑150 (MQ=255)
                                                                                                              aGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGtt                                    <  1:63357/150‑1 (MQ=255)
                                                                                                               gACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTc                                   >  1:1781780/1‑150 (MQ=255)
                                                                                                                aCGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTca                                  <  2:3468300/150‑1 (MQ=255)
                                                                                                                   ccAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTcagcc                               >  1:5175135/1‑150 (MQ=255)
                                                                                                                   ccAAGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTcagcc                               <  1:4587563/150‑1 (MQ=255)
                                                                                                                   ccAAGGTNCAGCGGGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTcagnc                               <  1:1633201/150‑1 (MQ=255)
                                                                                                                     aaGTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCTAGTTcagccag                             <  1:2732267/150‑1 (MQ=255)
                                                                                                                       gTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCcagca                           <  2:1809511/150‑1 (MQ=255)
                                                                                                                       gTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCcagca                           <  2:4189416/150‑1 (MQ=255)
                                                                                                                       gTTCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCcagca                           >  2:3688017/1‑150 (MQ=255)
                                                                                                                       gTTCCAGCGTGGCCTGGAAGCGCGGCACTAACACTTCCCAAACCGGGATGCGGCTTTTCATTGAAAAGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTTCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCcagca                           >  2:844993/1‑150 (MQ=255)
                                                                                                                         tCCAGCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCagcagc                         <  2:4031487/150‑1 (MQ=255)
                                                                                                                             gCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTgcg                     <  2:4473390/150‑1 (MQ=255)
                                                                                                                             gCGTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTgcg                     >  1:223995/1‑150 (MQ=255)
                                                                                                                               gTGGCCTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTgcgca                   <  1:1453504/150‑2 (MQ=255)
                                                                                                                                   ccTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGAc               <  2:4810441/150‑1 (MQ=255)
                                                                                                                                    cTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACg              <  1:2483610/150‑1 (MQ=255)
                                                                                                                                    cTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACg              <  1:2844862/150‑1 (MQ=255)
                                                                                                                                    cTGGAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACg              <  1:3634334/150‑1 (MQ=255)
                                                                                                                                      ggAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGct            <  1:1895350/150‑1 (MQ=255)
                                                                                                                                      ggAAGCGCGGCACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGct            <  1:3354934/150‑1 (MQ=255)
                                                                                                                                                cACTAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGAt  <  1:2588656/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATAACCTGTCAGCGCCAGGCCAACCGCTGTGTGATCGAAAATGGAACCGCGTTTAACCGCAGCCAGCACGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGAT  >  CP009273/3698728‑3699021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑