Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,810,195 C→A 100% R54S (CGT→AGT)  yicC → UPF0701 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,810,1950CA99.6% 732.7 / ‑4.2 226R54S (CGT→AGT) yicCUPF0701 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (106/119);  total (107/119)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

ATGACCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGG  >  CP009273/3810048‑3810341
                                                                                                                                                   |                                                                                                                                                  
aTGACCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGt                                                                                                                                                  >  2:2379923/1‑150 (MQ=255)
  gACCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTtc                                                                                                                                                <  2:2779496/150‑1 (MQ=255)
  gACCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTtc                                                                                                                                                >  1:4884655/1‑150 (MQ=255)
    ccGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTtctc                                                                                                                                              <  2:5325602/150‑1 (MQ=255)
       ccTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGcc                                                                                                                                           <  1:3279502/150‑1 (MQ=255)
         tACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTg                                                                                                                                         >  1:916291/1‑150 (MQ=255)
            gCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcg                                                                                                                                      <  2:3306833/150‑1 (MQ=255)
              ccGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGAcgcg                                                                                                                                    <  1:537481/150‑1 (MQ=255)
                 gCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCgg                                                                                                                                 <  2:4634132/150‑1 (MQ=255)
                  cGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGt                                                                                                                                >  2:4357900/1‑150 (MQ=255)
                   gTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTa                                                                                                                               >  2:5045076/1‑150 (MQ=255)
                    tGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTaa                                                                                                                              <  1:5181152/150‑1 (MQ=255)
                      aaaTCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAg                                                                                                                            >  1:2401903/1‑150 (MQ=255)
                         tCAAGTGTGAATGGGGGAGCGCAACCTGGGAAAAGCGCTCGGTAAACCAGCGTAATCTGCAAACTAACTCTCGTCTGCCGGAGCAGTTCCGGAGCCTTCAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         >  2:746510/1‑150 (MQ=255)
                         tCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         <  1:5080779/150‑1 (MQ=255)
                         tCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         <  2:1285037/150‑1 (MQ=255)
                         tCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         <  1:1001220/150‑1 (MQ=255)
                         tCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTgg                                                                                                                         >  2:1393654/1‑150 (MQ=255)
                           aaGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGaa                                                                                                                       <  1:1702375/150‑1 (MQ=255)
                            aGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAAt                                                                                                                      >  1:2154499/1‑150 (MQ=255)
                            aGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAAt                                                                                                                      >  2:3418528/1‑150 (MQ=255)
                             gggTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATg                                                                                                                     <  2:4077073/150‑1 (MQ=255)
                             gggTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATg                                                                                                                     <  2:4076493/150‑1 (MQ=255)
                             gggTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATg                                                                                                                     >  2:5120850/1‑150 (MQ=255)
                              ggTGAATGGGGGAGCGCAACCTGGGAAGTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGt                                                                                                                    >  1:1649675/1‑150 (MQ=255)
                              ggTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGt                                                                                                                    >  1:569988/1‑150 (MQ=255)
                                  aaTGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTAccc                                                                                                                <  1:3743398/150‑1 (MQ=255)
                                  aaTGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTAccc                                                                                                                <  1:2805140/150‑1 (MQ=255)
                                   aTGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCt                                                                                                               <  1:2641237/150‑1 (MQ=255)
                                     gggggAGCGCACCCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTgc                                                                                                             <  2:4399549/150‑1 (MQ=255)
                                     gggggAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTgc                                                                                                             <  2:3367480/150‑1 (MQ=255)
                                      ggggAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTgcg                                                                                                            >  2:1597672/1‑150 (MQ=255)
                                        ggAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCt                                                                                                          <  1:3464713/150‑1 (MQ=255)
                                         gAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTa                                                                                                         >  2:3539380/1‑150 (MQ=255)
                                          aGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCGAt                                                                                                        >  1:468949/1‑150 (MQ=255)
                                            cgcAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGa                                                                                                      <  1:4469008/150‑1 (MQ=255)
                                            cgcAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTAGCCTGACGCGCGGTAAATTGGAATGTAACCTGCGCTATGa                                                                                                      >  1:954553/1‑150 (MQ=255)
                                             gcAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAg                                                                                                     >  1:2873360/1‑150 (MQ=255)
                                              cAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGc                                                                                                    >  1:3585825/1‑150 (MQ=255)
                                                 ccTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACGTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAg                                                                                                 <  2:532425/150‑1 (MQ=255)
                                                  cTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGa                                                                                                <  2:2123943/150‑1 (MQ=255)
                                                   tGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGTGCAGTTCCGTAGCCTTGTACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGAt                                                                                               <  2:572662/150‑1 (MQ=255)
                                                    gggAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATg                                                                                              <  2:196821/150‑1 (MQ=255)
                                                       aaaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTa                                                                                           >  2:3241119/1‑150 (MQ=255)
                                                       aaaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCGATGAGCCAGATGTTa                                                                                           >  1:615770/1‑150 (MQ=255)
                                                       aaaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGGAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTa                                                                                           >  2:3794398/1‑150 (MQ=255)
                                                        aaTGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAg                                                                                          <  2:2302909/150‑1 (MQ=255)
                                                          tGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAgcg                                                                                        <  2:1761011/150‑1 (MQ=255)
                                                           gcgcTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAgcgc                                                                                       <  1:1328632/150‑1 (MQ=255)
                                                            cgcTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGAATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAgcgcg                                                                                      >  2:2905837/1‑150 (MQ=255)
                                                              cTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCa                                                                                    <  2:1685823/150‑1 (MQ=255)
                                                              cTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCa                                                                                    >  2:4483469/1‑150 (MQ=255)
                                                                 ggTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAgg                                                                                 <  2:4959102/150‑1 (MQ=255)
                                                                   tAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTg                                                                               <  2:4270418/150‑1 (MQ=255)
                                                                    aaaCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGa                                                                              <  2:2144448/150‑1 (MQ=255)
                                                                      aCCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTCGCGCGCAAGGTGAGc                                                                            >  2:1993850/1‑150 (MQ=255)
                                                                         aGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGa                                                                         >  1:395222/1‑150 (MQ=255)
                                                                             ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGACCCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     <  2:814194/150‑1 (MQ=255)
                                                                             ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     <  1:4289581/150‑1 (MQ=255)
                                                                             ttATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCt                                                                     <  1:1421919/150‑1 (MQ=255)
                                                                               aTCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCa                                                                   >  2:3874353/1‑150 (MQ=255)
                                                                               aTCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCa                                                                   >  2:4903890/1‑150 (MQ=255)
                                                                                tCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCaa                                                                  <  1:2119533/150‑1 (MQ=255)
                                                                                 cTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAAc                                                                 <  1:434908/150‑1 (MQ=255)
                                                                                  tGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCCGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACg                                                                >  2:2469611/1‑150 (MQ=255)
                                                                                     aaaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaa                                                             >  2:360227/1‑150 (MQ=255)
                                                                                     aaaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaa                                                             >  2:3611179/1‑150 (MQ=255)
                                                                                     aaaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaa                                                             <  2:1859159/150‑1 (MQ=255)
                                                                                      aaCTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGaaaa                                                            <  1:5120961/150‑1 (MQ=255)
                                                                                         ttacttTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACt                                                         >  1:1779913/1‑150 (MQ=255)
                                                                                             tttCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCt                                                     >  1:2208279/1‑150 (MQ=255)
                                                                                                 gTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAAc                                                 >  1:2651773/1‑150 (MQ=255)
                                                                                                 gTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAAc                                                 >  2:2608644/1‑150 (MQ=255)
                                                                                                  tCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACa                                                <  1:1565632/150‑1 (MQ=255)
                                                                                                   cTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAg                                               >  2:4467342/1‑150 (MQ=255)
                                                                                                        ggAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGGTCGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGt                                          >  2:911978/1‑150 (MQ=255)
                                                                                                         gAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTa                                         <  1:3772461/150‑1 (MQ=255)
                                                                                                           gCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAAc                                       <  2:3100867/150‑1 (MQ=255)
                                                                                                            cAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACt                                      <  2:3585825/150‑1 (MQ=255)
                                                                                                                   gTAGCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGAGCCTCAACGAAAAACTGGCTAAACAGCTGGTAACAGCCGCGa                               >  2:1888680/1‑150 (MQ=255)
                                                                                                                      gCCTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACt                            <  1:3600118/150‑1 (MQ=255)
                                                                                                                        cTTGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTgg                          >  2:5234614/1‑150 (MQ=255)
                                                                                                                         ttGAACCTGTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTggg                         <  2:4344620/150‑1 (MQ=255)
                                                                                                                                 gTCGTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGGAAAAATg                 >  1:889838/1‑150 (MQ=255)
                                                                                                                                 gTCGTTCGCGAGCCTATAAGTACTCGCCTGACGCGCGGAAAAGTGGAATGTACCCTGCGCTCTGAGCCAGATGTAAGCGCGCAAGGGGAGCTGAGCCTCTACGAAAAACTGGCTAAACCGCTGGTAACGGCCGCGACCAGGGTAAAAATg                 >  2:113372/1‑150 (MQ=255)
                                                                                                                                    gTTCGCGAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCag              >  1:3647690/1‑150 (MQ=255)
                                                                                                                                        gcgAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTg          <  1:4665107/150‑1 (MQ=255)
                                                                                                                                          gAGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTCGCGCGCAAGGTGAGCTGATACTCAACGAAAAACTGGCTAAACATCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGAc        >  1:1836548/1‑150 (MQ=255)
                                                                                                                                           aGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACg       >  1:320804/1‑150 (MQ=255)
                                                                                                                                           aGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACg       <  2:2741468/150‑1 (MQ=255)
                                                                                                                                           aGCGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACg       >  1:776635/1‑150 (MQ=255)
                                                                                                                                             cGTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGaa     >  2:5160518/1‑150 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGCCGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    <  2:1329534/150‑1 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    <  2:5121367/150‑1 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  2:3638708/1‑150 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  2:4245372/1‑150 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  2:3251692/1‑150 (MQ=255)
                                                                                                                                              gTATTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAg    >  2:3275785/1‑150 (MQ=255)
                                                                                                                                                aTTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAggg  >  1:2955826/1‑150 (MQ=255)
                                                                                                                                                aTTAGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAggg  >  1:2280999/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGACCGCCTACGCCCGGCGTGAAATCAAGGGTGAATGGGGGAGCGCAACCTGGGAAATGCGCTCGGTAAACCAGCGTTATCTGGAAACTTACTTTCGTCTGCCGGAGCAGTTCCGTAGCCTTGAACCTGTCGTTCGCGAGCGTATTCGTTCTCGCCTGACGCGCGGTAAAGTGGAATGTACCCTGCGCTATGAGCCAGATGTTAGCGCGCAAGGTGAGCTGATCCTCAACGAAAAACTGGCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGG  >  CP009273/3810048‑3810341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑