BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,339	A→C	6.6%	Q12P (CAA→CCA) ‡	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,339	0	A	C	6.6%	558.3 / 2.2	265	Q12P (CAA→CCA) ‡	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (126/112); new base C (24/3); total (150/115)
Fisher's exact test for biased strand distribution p-value = 3.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

 atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      >  1:404311/1‑150 (MQ=255)

  tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                     <  1:3462841/150‑1 (MQ=255)

       aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                >  2:889519/1‑150 (MQ=255)

        aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                               >  1:304923/1‑150 (MQ=255)

         aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                              <  1:2364437/150‑1 (MQ=255)

          aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCGTTgcg                                                                                                                                             >  1:3297831/1‑150 (MQ=255)

           gTGCATTTATCTCATTTAAATATTTAGATGATGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCTAGTCCGAAAATGCGCAACCCGTTgagc                                                                                                                                            >  2:2960650/1‑150 (MQ=255)

              cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGa                                                                                                                                         >  1:1583004/1‑150 (MQ=255)

               aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        <  2:236846/150‑1 (MQ=255)

                tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                       <  2:2148712/150‑1 (MQ=255)

                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCa                                                                                                                                     >  1:5237786/1‑150 (MQ=255)

                  tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCATTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGAGCAGACTCa                                                                                                                                     >  1:1027874/1‑150 (MQ=255)

                    tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAcaccaa                                                                                                                                   >  2:545996/1‑145 (MQ=255)

                       cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                >  2:4359970/1‑150 (MQ=255)

                       cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAAGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACa                                                                                                                                >  2:3486956/1‑150 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAg                                                                                                                               >  1:2222971/1‑150 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACACAAAACAg                                                                                                                               >  2:2026310/1‑150 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  2:1711043/1‑150 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               >  1:4336502/1‑150 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                               <  2:481655/150‑1 (MQ=255)

                        aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAg                                                                                                                               >  2:1470901/1‑150 (MQ=255)

                           tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                            >  2:4656561/1‑150 (MQ=255)

                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          >  2:3940473/1‑150 (MQ=255)

                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGacc                                                                                                                          >  1:4720693/1‑148 (MQ=255)

                             aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCAACCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAAc                                                                                                                          >  2:2741471/1‑150 (MQ=255)

                                atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                       >  2:391621/1‑150 (MQ=255)

                                atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGa                                                                                                                       >  1:1250763/1‑150 (MQ=255)

                                    aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGGTTTa                                                                                                                   >  2:2262/1‑150 (MQ=255)

                                       tgtggttgtgGAAAACGTTTGCTTTTCTCTTTCGGCCAGCTAAAAGGCCCGCTGTGCTACCTCGGTATTGTTTTCGTTGTCTGTTCCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                <  1:3375231/144‑1 (MQ=255)

                                       tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTTCGCAGTCTAAAAACAGCGAACTGATTTACCg                                                                                                                >  1:4803518/1‑150 (MQ=255)

                                         aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTGTGCTACCAGGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  1:3940306/150‑1 (MQ=255)

                                          ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                             <  2:3031239/150‑1 (MQ=255)

                                             tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                          >  1:3078302/1‑150 (MQ=255)

                                               tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg                                                                                                        <  2:2504613/150‑1 (MQ=255)

                                                  cAAACTTTTGCTTTTCACTGTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCGCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  2:4762430/150‑1 (MQ=255)

                                                  cAAACTTTTGCTTTTATCTTTCGTTCAGATACAAGGCCCGCTGTGCCACCACGGGATTGTTTTCGATGTCTGTGTCCACCATCGAGTAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  2:101928/150‑1 (MQ=255)

                                                  cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGATTTAACGTCTTGAAGATc                                                                                                     >  1:2843769/1‑150 (MQ=255)

                                                  aaaaCTTTTGCTTTTATATTTCGTTCTGCTAAATGGCCCGCTTGGCTACCTCGTTATTTTTTTCTATGTTTGTTTCCACCCTGTTTTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  2:740990/149‑1 (MQ=255)

                                                   aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAATGAACAGATTTAACGGCTTGAAGAtag                                                                                                    >  1:1319595/1‑148 (MQ=255)

                                                      cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 >  2:4121867/1‑150 (MQ=255)

                                                       gTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                                <  2:3369362/150‑1 (MQ=255)

                                                         ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                              <  1:4459243/150‑1 (MQ=255)

                                                            cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAATCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                           >  2:674237/1‑150 (MQ=255)

                                                             ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGATTTACCGTCTTGAAGATAGTccgccccat                                                                                          >  2:3747638/1‑146 (MQ=255)

                                                             tttgCTCTTGCGGTCAGATCAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCGGTTCCCCCCCTCTCGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          <  2:4194088/150‑1 (MQ=255)

                                                              tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         >  2:3543453/1‑150 (MQ=255)

                                                               ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGGCTTGAATATCGTCCGCCGCTTcc                                                                                        >  1:1053812/1‑150 (MQ=255)

                                                                tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       >  2:2075148/1‑150 (MQ=255)

                                                                ccgctTGCGCTCCGCTCAAAGGCCCGCTTTGCTACCACGGGAGTGTGTTCGCTGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       <  2:4078106/147‑1 (MQ=255)

                                                                 ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                      >  1:3517667/1‑150 (MQ=255)

                                                                   ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                                    >  1:740959/1‑150 (MQ=255)

                                                                       cgctCAGATAAAATGCCCGCTTTGCTACCTCGGGTTTTTTTTCTATGTCTTTTTCCCCCCTCGTTACATAATCTGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:1563242/147‑1 (MQ=255)

                                                                       cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCCAAACAGCGAACTGCTTTACCGTCTTGAAGATCGTCCGCCGCGGCCTCAAAccc                                                                                >  2:5070217/1‑150 (MQ=255)

                                                                        ggTCAGCTAAAAGGCCCGCGTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCCCCCTCGAGTCATAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCATACCCt                                                                               <  2:2119518/150‑1 (MQ=255)

                                                                               tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGCTTTACCGTCGTGAAGATCGTCCGCCGCGTCCACAAACCCGGTTTgcc                                                                        >  2:4706634/1‑150 (MQ=255)

                                                                                aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTgccg                                                                       >  2:786060/1‑150 (MQ=255)

                                                                                 aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGGCTTGAAGAACGTCCGCCGCATCCTCAAACCCAGTTTgccgc                                                                      >  1:2142734/1‑150 (MQ=255)

                                                                                  aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCGCCAACCCTGTTTgccgcc                                                                     >  2:3186193/1‑150 (MQ=255)

                                                                                    tGCCCGCTTTGCTACCAGGGTTTTGTTTTCGATGTCTGTTCCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTg                                                                   <  1:2934339/150‑1 (MQ=255)

                                                                                     gCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                  >  2:1642471/1‑150 (MQ=255)

                                                                                       ccGCTTTGCTACCTGGGTTTTGTTTTCGATTTCTGTTACCCCCCTCGAGACACAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                <  1:3890566/150‑1 (MQ=255)

                                                                                        cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCCCGAGACATAAAATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                               <  2:2657527/150‑1 (MQ=255)

                                                                                           tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAt                                                            <  2:1261946/150‑1 (MQ=255)

                                                                                             tGCTCCCACGGTATTGTTTCCGCGGACTGTTCCCACCCCCGAGCCCCAAAATGCCCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  1:282189/150‑1 (MQ=255)

                                                                                               cTACCACGGGATTGTTTTCGTTGTCTGTTACCACCCTCGAGACATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        <  1:3111987/150‑1 (MQ=255)

                                                                                                tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACACAAAAAAGCGAACAGATTTACCGTCTTGAATATAGTCCGCCGCTGACTCAAACCCAGTTTGCCGCCAGTCAGCAActgct                                                       >  1:1897846/1‑150 (MQ=255)

                                                                                                 aCCACGGGTTTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgttg                                                      <  2:1412463/150‑1 (MQ=255)

                                                                                                 aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACAGATTTACCGTCTTGAATATAGTCCGCCGCTTCCTCAAACCCAGTTTTGCGCCAGTCAGCATctgctg                                                      >  1:3689973/1‑150 (MQ=255)

                                                                                                   cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                    >  2:4103143/1‑150 (MQ=255)

                                                                                                         aTTTTTTTCGATGTCTGTTACCACCCTCGAGCCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                              <  2:2514971/150‑1 (MQ=255)

                                                                                                         aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGCTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                              >  2:3356386/1‑150 (MQ=255)

                                                                                                          ttGTTTTCTATGTCTGTAACCACCCCCGAGCCATAAAATGCGACCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  2:2455287/150‑1 (MQ=255)

                                                                                                          ttGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  1:4202391/150‑1 (MQ=255)

                                                                                                            gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCGGTCAGCATCTGCTGGCGATGTTCGt                                           >  2:1111015/1‑150 (MQ=255)

                                                                                                               ttCTATGTCTGTTACCACCCTCTAGTCATAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        <  1:889519/150‑1 (MQ=255)

                                                                                                                tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACACCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCGACCGCAAAACCAGTTTGCCGCCAGTCAGCAACATCAGGCGATGGTAGTTGCg                                       >  1:3183514/1‑150 (MQ=255)

                                                                                                                tCGATGTCTGTGTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAACCAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       <  2:470935/150‑1 (MQ=255)

                                                                                                                    tGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   <  2:3297831/150‑1 (MQ=255)

                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                   <  2:3106284/150‑1 (MQ=255)

                                                                                                                    tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTGCCGCCGGTCAGCATCTGCTGGCGAGGTTCGTTGCGGTGa                                   >  1:5251150/1‑150 (MQ=255)

                                                                                                                     gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAACTGCTGGCGATGTTCGTTGCGGGGAt                                  >  2:5194706/1‑150 (MQ=255)

                                                                                                                      tgtgtccccACCCTGGTGTCTGACTAGGCCCATCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  1:5319514/143‑1 (MQ=255)

                                                                                                                      tCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                 <  2:5154426/150‑1 (MQ=255)

                                                                                                                       cTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  2:5237786/150‑1 (MQ=255)

                                                                                                                        tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACAGATTTACCGTCTTGAAGATAGTCCGCCCCGTCCACAAAACCAGTTTTCCGCCAGTCAGCAACAGCAGGCGAAGTTAGTTGCGGTGATCAc                               >  1:1675951/1‑150 (MQ=255)

                                                                                                                         gTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACACAAAACAGCGAACTGATTTACCCTCTTGAATATAGTCCGCCGCGTACTCAAACCCTGTTTGCCGCCTGTCAGCCTCTGCTGGCGATGTTCGTTGCGGTGATAACg                              >  2:2434271/1‑150 (MQ=255)

                                                                                                                                     gAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCATCAGCTGGCGATGTTAGTTTCGGTGATCACGCCAGCGCTATTa                  >  2:1962940/1‑150 (MQ=255)

                                                                                                                                     gAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTTCGGTGATCACGCCAGCGCTATTa                  >  1:4373584/1‑150 (MQ=255)

                                                                                                                                       gtaataaaaGGCGCAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  1:2056442/145‑1 (MQ=255)

                                                                                                                                       gTCAGAAAATGCGCAACCGGTTGCGCAGTCTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  1:2617913/150‑1 (MQ=255)

                                                                                                                                         cAGAAAATGCTCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  2:1776973/150‑1 (MQ=255)

                                                                                                                                          ataaacTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  2:4982274/148‑1 (MQ=255)

                                                                                                                                          ataaaaTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  1:4304171/148‑1 (MQ=255)

                                                                                                                                             acaTGCGCACCCGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  2:4471521/148‑1 (MQ=255)

                                                                                                                                             aaaTGCGCCCCCGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:4933911/150‑1 (MQ=255)

                                                                                                                                               aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg        <  2:4300668/150‑1 (MQ=255)

                                                                                                                                                tGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc       <  2:3181221/150‑1 (MQ=255)

                                                                                                                                                 gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg      <  2:3411741/150‑1 (MQ=255)

                                                                                                                                                     aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:2022094/1‑150 (MQ=255)

                                                                                                                                                     aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:157817/1‑150 (MQ=255)

TATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822190‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`