Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,340 A→C 16.5% Q12H (CAA→CAC) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3400AC16.5% 441.8 / 26.7 265Q12H (CAA→CAC) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (142/72);  new base C (8/42);  total (150/115)
Fisher's exact test for biased strand distribution p-value = 7.33e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822191‑3822489
                                                                                                                                                     |                                                                                                                                                     
atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                       >  1:404311/1‑150 (MQ=255)
atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                       >  1:2213888/1‑150 (MQ=255)
 tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                      <  1:3462841/150‑1 (MQ=255)
       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                >  1:608488/1‑150 (MQ=255)
       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                <  1:2233053/150‑1 (MQ=255)
         aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                              >  1:3308340/1‑150 (MQ=255)
          gTGCATTTATCTCATTTAAATATTTAGATGATGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCTAGTCCGAAAATGCGCAACCCGTTgagc                                                                                                                                             >  2:2960650/1‑150 (MQ=255)
          gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcgc                                                                                                                                             >  2:862702/1‑150 (MQ=255)
           tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCa                                                                                                                                            >  2:5296998/1‑150 (MQ=255)
             cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGa                                                                                                                                          >  2:4087948/1‑150 (MQ=255)
              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         <  2:236846/150‑1 (MQ=255)
              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGAc                                                                                                                                         >  1:3161907/1‑150 (MQ=255)
              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGAc                                                                                                                                         >  1:2715642/1‑150 (MQ=255)
               tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                        >  2:2075757/1‑150 (MQ=255)
               tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                        <  2:2148712/150‑1 (MQ=255)
                 tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCATTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGAGCAGACTCa                                                                                                                                      >  1:1027874/1‑150 (MQ=255)
                  aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACaa                                                                                                                                     >  1:5249339/1‑150 (MQ=255)
                      cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                 >  2:3486963/1‑150 (MQ=255)
                      cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                                 >  2:4800793/1‑150 (MQ=255)
                      cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCATTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCCCAACCGGGTGCGCAGACTCAAAACa                                                                                                                                 >  2:520274/1‑150 (MQ=255)
                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                >  1:4336502/1‑150 (MQ=255)
                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAg                                                                                                                                >  2:1470901/1‑150 (MQ=255)
                        tttAAATTTTTATATTAGGATGTGGCAAACTTTTGTTTTTCTATTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGc                                                                                                                               <  2:698685/150‑1 (MQ=255)
                          tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                             >  1:3097873/1‑150 (MQ=255)
                          tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGa                                                                                                                             >  2:4656561/1‑150 (MQ=255)
                           aaaTATTTAGATGAGTATGTGGCTATCGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTTCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATGGCGAAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                            <  2:305508/150‑1 (MQ=255)
                            aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACACAAAACACCGAAc                                                                                                                           >  1:2051911/1‑150 (MQ=255)
                            aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCAACCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAAc                                                                                                                           >  2:2741471/1‑150 (MQ=255)
                             atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACAGCGCACt                                                                                                                          >  1:4144001/1‑150 (MQ=255)
                               atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                        >  2:391796/1‑150 (MQ=255)
                               atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGa                                                                                                                        >  1:1250763/1‑150 (MQ=255)
                                 ttAGATGAGGTTGTGCCAAACTTTTGCTTTACTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAACCCGGTTGCGCAGACTCAAAACAGCGAACTGAtt                                                                                                                      <  2:5044290/150‑1 (MQ=255)
                                  tAGATGTGGTTGTGTAAACCTTTTGCTTTTCTCTTTCGGTCCGCTAAAATGCCCGCTGTGCTACCACGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGGGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                     <  1:306153/150‑1 (MQ=255)
                                   aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACACCGAACGGATTTa                                                                                                                    >  1:4791423/1‑150 (MQ=255)
                                   aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGGTTTa                                                                                                                    >  2:2262/1‑150 (MQ=255)
                                    gATGTGGTTGTGGAAACGGTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGGTTTGCTACCTCGGTTTTGTTTTCGATGTCTGTTTCCACCCTGGAGTCTGAATATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                   <  2:4041295/150‑1 (MQ=255)
                                     aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTaac                                                                                                                  >  1:212013/1‑148 (MQ=255)
                                      tgtggttgtgGAAAACGTTTGCTTTTCTCTTTCGGCCAGCTAAAAGGCCCGCTGTGCTACCTCGGTATTGTTTTCGTTGTCTGTTCCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                 <  1:3375231/144‑1 (MQ=255)
                                       gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                                <  2:2654313/150‑1 (MQ=255)
                                       gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACAAAAAACAGACAACAGATTTAACgg                                                                                                                >  1:4521980/1‑149 (MQ=255)
                                        aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTGTGCTACCAGGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                               <  1:3940306/150‑1 (MQ=255)
                                         ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                              >  2:1698584/1‑150 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTTACGTCtt                                                                                                             >  2:1766606/1‑150 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                             >  2:1099474/1‑150 (MQ=255)
                                              tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg                                                                                                         <  2:2504613/150‑1 (MQ=255)
                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAATGAACAGATTTAACGGCTTGAAGAtag                                                                                                     >  1:1319595/1‑148 (MQ=255)
                                                     ggTTTGCTTTTCTGTGGCGGCCTGCTAAAACGCCCGCTTTGCTACCTCGGTTTTTTTTTCGATGTCTGTTCCCACCCTCGAGACACAAAATGCGCACCGGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                  <  1:3184011/149‑1 (MQ=255)
                                                     cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCCGTTGCGCAGACTCAAAACAACGAACAGATTTAACCTCTTGAAGATCGTcc                                                                                                  >  1:938865/1‑150 (MQ=255)
                                                     cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTACAAACAGCGAACTGATTTAACCTCTTGTAGATAGTcc                                                                                                  >  1:5090109/1‑150 (MQ=255)
                                                       tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGGAGATCGTccgc                                                                                                >  2:1373230/1‑150 (MQ=255)
                                                        ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTTCGCAGACTCAAAACACCGAACAGATTTACCGTCGTGAAGCTCGTccgcc                                                                                               >  2:1852175/1‑150 (MQ=255)
                                                        gtGCTTTTCTCTTTCGGTCAGCTAAACGGCCCGTTTTGCTACCCGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAACTGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgcc                                                                                               <  1:5306924/149‑1 (MQ=255)
                                                           cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAATCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                            >  2:674237/1‑150 (MQ=255)
                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAATCGAACAGATTTACCGTCTTGTAGATAGTCCGCCGCtt                                                                                           >  1:2984919/1‑150 (MQ=255)
                                                            tttgCTCTTGCGGTCAGATCAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCGGTTCCCCCCCTCTCGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                           <  2:4194088/150‑1 (MQ=255)
                                                             tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                          >  2:3543453/1‑150 (MQ=255)
                                                              ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGGCTTGAATATCGTCCGCCGCTTcc                                                                                         >  1:1053812/1‑150 (MQ=255)
                                                                  ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                                     >  1:740963/1‑150 (MQ=255)
                                                                       ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAATATAGTACGCCGCGTCCTCAAACCCt                                                                                >  1:2580772/1‑150 (MQ=255)
                                                                              tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGCTTTACCGTCGTGAAGATCGTCCGCCGCGTCCACAAACCCGGTTTgcc                                                                         >  2:4706634/1‑150 (MQ=255)
                                                                                 aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                      >  2:4737784/1‑150 (MQ=255)
                                                                                 aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCGCCAACCCTGTTTgccgcc                                                                      >  2:3186193/1‑150 (MQ=255)
                                                                                  aTGCCCGCTTTGCTACCACGGTTTTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                     <  2:3815963/150‑1 (MQ=255)
                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTc                                                                  >  2:3395050/1‑150 (MQ=255)
                                                                                      ccGCTTTGCTACCTGGGTTTTGTTTTCGATTTCTGTTACCCCCCTCGAGACACAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                 <  1:3890566/150‑1 (MQ=255)
                                                                                         cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCGGGTGCGCAGACTCAAAACAGCGAAAAGTTTTAACCTCTTGAAGATAGTCAGCCGCGTACTCAAACCCTGGTTGCCGCCGGTCAGCa                                                              >  2:4899292/1‑150 (MQ=255)
                                                                                             gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCATGAAGATCGTCCGCCGCATCCTCAAACCCTGTTTGCCGCCGGTCCGCATctg                                                          >  2:1997423/1‑150 (MQ=255)
                                                                                               tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACACAAAAAAGCGAACAGATTTACCGTCTTGAATATAGTCCGCCGCTGACTCAAACCCAGTTTGCCGCCAGTCAGCAActgct                                                        >  1:1897846/1‑150 (MQ=255)
                                                                                                aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACAGATTTACCGTCTTGAATATAGTCCGCCGCTTCCTCAAACCCAGTTTTGCGCCAGTCAGCATctgctg                                                       >  1:3689973/1‑150 (MQ=255)
                                                                                                 ccccgtgATTTTTTTCGTTGTCTGTTTCCACCCTCTCGTAACAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  1:1571452/144‑1 (MQ=255)
                                                                                                  cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCATGAAGATCGTCCCCCGCTTCCTCAAACCCTGTTTTGCGCCTGTCAGCATCTGCTGGc                                                     >  2:4103848/1‑150 (MQ=255)
                                                                                                        aTTTTTTTCGATGTCTGTTACCACCCTCGAGCCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                               <  2:2514971/150‑1 (MQ=255)
                                                                                                        aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                               <  2:2127854/150‑1 (MQ=255)
                                                                                                        aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGCTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                               >  2:3356386/1‑150 (MQ=255)
                                                                                                         ttGTTTTCTATGTCTGTAACCACCCCCGAGCCATAAAATGCGACCCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                              <  2:2455287/150‑1 (MQ=255)
                                                                                                               tCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                        <  2:4134952/150‑1 (MQ=255)
                                                                                                                cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGTTGCGCAGACAAAAAAAAAAGAACTTATTTAAAGACTTTAAAATAGTCCGCCGAGTCCAAAAAAACTGTTTGCAGCCAGTCAGAATCTGCTGGGGATTTTCGTTTCgg                                       >  1:1476640/1‑150 (MQ=255)
                                                                                                                   tGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                    <  2:3297831/150‑1 (MQ=255)
                                                                                                                   tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                    <  2:3106284/150‑1 (MQ=255)
                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTACGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                   >  2:5194582/1‑150 (MQ=255)
                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAAAAGCCAACTGATTTAACGTCTTGAATATCGTCCGCCGCGTCCTCAAACCCTGTTTGGCGCCAGTCAGCATCAGCTGGCGATGTTAGTTGCggggtt                                   >  1:3639142/1‑146 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACAGATTTACCGTCTTGAAGATAGTCCGCCCCGTCCACAAAACCAGTTTTCCGCCAGTCAGCAACAGCAGGCGAAGTTAGTTGCGGTGATCAc                                >  1:1675951/1‑150 (MQ=255)
                                                                                                                        gTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACACAAAACAGCGAACTGATTTACCCTCTTGAATATAGTCCGCCGCGTACTCAAACCCTGTTTGCCGCCTGTCAGCCTCTGCTGGCGATGTTCGTTGCGGTGATAACg                               >  2:2434271/1‑150 (MQ=255)
                                                                                                                                   cGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                    <  1:4440180/150‑1 (MQ=255)
                                                                                                                                    gAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTTAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTTCGGTGATCACGCCAGCGCTATTa                   >  1:4373584/1‑150 (MQ=255)
                                                                                                                                      gTCAGAAACTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                 <  2:4494334/150‑1 (MQ=255)
                                                                                                                                        cAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt               <  2:754939/150‑1 (MQ=255)
                                                                                                                                        cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt               <  2:111796/150‑1 (MQ=255)
                                                                                                                                        cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAATGATTTACCGTCTTGAATATCGTCCGCCGCGTCCTCAAACCCGGTTTGCCGCCAGTCAGCAAATGCTGGCGATGTTAGGTGCGGTGATCACGCCAGCGCTCTTAATCt               >  2:5090367/1‑150 (MQ=255)
                                                                                                                                         ataaaaTGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg              <  1:4304171/148‑1 (MQ=255)
                                                                                                                                          taaacTGCGCACCCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc             <  1:201566/149‑1 (MQ=255)
                                                                                                                                          gAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc             <  1:2888108/150‑1 (MQ=255)
                                                                                                                                            aaaTGCGCCCCCGGTGCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           <  1:4933911/150‑1 (MQ=255)
                                                                                                                                              aTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg         <  1:2833086/150‑1 (MQ=255)
                                                                                                                                              aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg         <  2:4300668/150‑1 (MQ=255)
                                                                                                                                               tGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgc        <  2:3181221/150‑1 (MQ=255)
                                                                                                                                                gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg       <  2:4411276/150‑1 (MQ=255)
                                                                                                                                                gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg       <  2:3411792/150‑1 (MQ=255)
                                                                                                                                                    aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg   >  2:157817/1‑150 (MQ=255)
                                                                                                                                                     aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTggg  <  2:1608391/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  CP009273/3822191‑3822489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑