Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,828,206 T→A 100% S362C (AGT→TGT)  yicJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,828,2060TA99.4% 1111.8 / ‑3.1 333S362C (AGT→TGT) yicJputative transporter
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (143/187);  total (144/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 335 total aligned reads displayed.

AATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAA  >  CP009273/3828057‑3828352
                                                                                                                                                     |                                                                                                                                                  
aaTCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACt                                                                                                                                                    >  2:4767552/1‑150 (MQ=255)
   cGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGAt                                                                                                                                                 <  1:3105640/150‑1 (MQ=255)
      gAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATccc                                                                                                                                              <  1:1689730/150‑1 (MQ=255)
       aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                             <  2:4025781/150‑1 (MQ=255)
       aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCg                                                                                                                                             >  2:4513954/1‑150 (MQ=255)
         tAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGt                                                                                                                                           <  1:1498311/150‑1 (MQ=255)
          aGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTc                                                                                                                                          >  2:5052196/1‑150 (MQ=255)
           gcgcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCa                                                                                                                                         <  1:3421170/150‑1 (MQ=255)
             gcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAgg                                                                                                                                       <  2:3059964/150‑1 (MQ=255)
             gcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAgg                                                                                                                                       <  2:1927444/150‑1 (MQ=255)
                ataatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGt                                                                                                                                    >  1:1491464/1‑150 (MQ=255)
                ataatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGt                                                                                                                                    >  1:3158240/1‑150 (MQ=255)
                 taatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                   <  1:1917919/150‑1 (MQ=255)
                 taatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                   <  2:1357147/150‑1 (MQ=255)
                  aatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGttt                                                                                                                                  <  2:3955036/150‑1 (MQ=255)
                    tgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAc                                                                                                                                <  2:127894/150‑1 (MQ=255)
                     gatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAcc                                                                                                                               <  2:3688337/150‑1 (MQ=255)
                        gCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCAtt                                                                                                                            <  1:3184250/150‑1 (MQ=255)
                         cTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTg                                                                                                                           >  2:3606432/1‑150 (MQ=255)
                          tAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGc                                                                                                                          <  2:650758/150‑1 (MQ=255)
                          tAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGc                                                                                                                          <  1:1374179/150‑1 (MQ=255)
                           aaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCCGGCGTTTACCATTGca                                                                                                                         >  1:2088410/1‑150 (MQ=255)
                            aTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcac                                                                                                                        >  2:342130/1‑150 (MQ=255)
                              cGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                      <  1:3922370/150‑1 (MQ=255)
                               gTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                     >  2:870284/1‑150 (MQ=255)
                               gTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                     >  1:4534464/1‑150 (MQ=255)
                               gTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcaccac                                                                                                                     >  1:2902175/1‑150 (MQ=255)
                                tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACt                                                                                                                    >  1:4150570/1‑150 (MQ=255)
                                tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACt                                                                                                                    <  1:440126/150‑1 (MQ=255)
                                tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACt                                                                                                                    <  2:5192977/150‑1 (MQ=255)
                                   cgcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                                 >  2:24465/1‑150 (MQ=255)
                                     cTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCg                                                                                                               <  2:3349770/150‑1 (MQ=255)
                                       gTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTa                                                                                                             >  2:780295/1‑150 (MQ=255)
                                       gTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTa                                                                                                             >  2:5130197/1‑150 (MQ=255)
                                        ttCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAg                                                                                                            <  2:25786/150‑1 (MQ=255)
                                          cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                          >  2:3902679/1‑150 (MQ=255)
                                          cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                          <  1:210457/150‑1 (MQ=255)
                                          cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                          >  1:3676506/1‑150 (MQ=255)
                                           tGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCg                                                                                                         <  1:2031401/150‑1 (MQ=255)
                                           tGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCg                                                                                                         <  2:593725/150‑1 (MQ=255)
                                              gcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                      <  2:3272201/150‑1 (MQ=255)
                                                 ttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTa                                                                                                   >  2:3489070/1‑150 (MQ=255)
                                                 ttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTa                                                                                                   >  1:2691251/1‑150 (MQ=255)
                                                    tCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCg                                                                                                <  1:4048885/150‑1 (MQ=255)
                                                        ccGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAca                                                                                            >  2:2675972/1‑150 (MQ=255)
                                                           catcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatca                                                                                         <  2:2812945/150‑1 (MQ=255)
                                                             tcatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCAtt                                                                                       <  1:675245/150‑1 (MQ=255)
                                                               atatCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAc                                                                                     >  1:418012/1‑150 (MQ=255)
                                                               atatCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAc                                                                                     <  1:326986/150‑1 (MQ=255)
                                                                           aGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAgg                                                                         >  2:10874/1‑150 (MQ=255)
                                                                           aGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAgg                                                                         <  1:1216824/150‑1 (MQ=255)
                                                                               aGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTc                                                                     >  1:1012876/1‑150 (MQ=255)
                                                                                  aTCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAcc                                                                  <  1:591958/150‑1 (MQ=255)
                                                                                  aTCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAcc                                                                  <  1:5066323/150‑1 (MQ=255)
                                                                                  aTCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAcc                                                                  >  2:4342148/1‑150 (MQ=255)
                                                                                       gCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTg                                                             <  2:793559/150‑1 (MQ=255)
                                                                                         cGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGAt                                                           <  1:559947/150‑1 (MQ=255)
                                                                                         cGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGAt                                                           >  1:2520563/1‑150 (MQ=255)
                                                                                          gATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATg                                                          <  2:182047/150‑1 (MQ=255)
                                                                                            tAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCa                                                        <  1:397831/150‑1 (MQ=255)
                                                                                             aaGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCaa                                                       >  2:1232723/1‑150 (MQ=255)
                                                                                              agagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAac                                                      >  1:1971703/1‑150 (MQ=255)
                                                                                                agCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                    >  2:4830615/1‑150 (MQ=255)
                                                                                                  cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                  <  1:421480/150‑1 (MQ=255)
                                                                                                  cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                  >  1:3021364/1‑150 (MQ=255)
                                                                                                   gccgccGAAGGCCAACCCCAGTTTGAGCACACACAGCGGGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACAcc                                                 >  2:2057660/1‑150 (MQ=255)
                                                                                                      gccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAt                                              <  1:5234124/150‑1 (MQ=255)
                                                                                                      gccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAt                                              <  2:3800123/150‑1 (MQ=255)
                                                                                                      gccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAt                                              >  1:3829496/1‑150 (MQ=255)
                                                                                                          aaGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                          >  2:1731229/1‑150 (MQ=255)
                                                                                                           aGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGa                                         >  1:856851/1‑150 (MQ=255)
                                                                                                            ggCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGaa                                        >  2:3236183/1‑150 (MQ=255)
                                                                                                            ggCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGaa                                        <  2:1491464/150‑1 (MQ=255)
                                                                                                             gCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAg                                       >  2:3152428/1‑150 (MQ=255)
                                                                                                             gCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAg                                       <  1:5016442/150‑1 (MQ=255)
                                                                                                              ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGa                                      <  1:363021/150‑1 (MQ=255)
                                                                                                                aaCCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATg                                    >  1:742188/1‑150 (MQ=255)
                                                                                                                 aCCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                   <  2:4177579/150‑1 (MQ=255)
                                                                                                                       gTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACaa                             >  2:2958959/1‑150 (MQ=255)
                                                                                                                       gTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACaa                             <  2:1198204/150‑1 (MQ=255)
                                                                                                                         ttGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAAc                           <  1:3489130/150‑1 (MQ=255)
                                                                                                                          tGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACa                          <  2:2742007/150‑1 (MQ=255)
                                                                                                                           gAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACAt                         <  2:4908099/150‑1 (MQ=255)
                                                                                                                            aGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATa                        >  1:4687891/1‑150 (MQ=255)
                                                                                                                             gCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAg                       <  2:2902289/150‑1 (MQ=255)
                                                                                                                             gCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAg                       <  2:1586846/150‑1 (MQ=255)
                                                                                                                               acaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTTGTCGACGGTTTCGGACTTCATGACCCTCTGGATAGGTGTCACCAGTTGATGCAACACTCCAATCACGAAGATGAAGACAAACATAGTg                     <  1:2382264/150‑1 (MQ=255)
                                                                                                                                caAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGa                    <  2:2498485/150‑1 (MQ=255)
                                                                                                                                 aaaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGAt                   >  2:1066213/1‑150 (MQ=255)
                                                                                                                                 aaaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGAt                   >  2:2115428/1‑150 (MQ=255)
                                                                                                                                  aaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATg                  <  2:1496481/150‑1 (MQ=255)
                                                                                                                                    cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCt                <  2:2279475/150‑1 (MQ=255)
                                                                                                                                     aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTg               >  1:193506/1‑150 (MQ=255)
                                                                                                                                       cGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGc             >  2:637746/1‑150 (MQ=255)
                                                                                                                                        gTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGcc            >  1:2199014/1‑150 (MQ=255)
                                                                                                                                         tGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCt           <  2:3021364/150‑1 (MQ=255)
                                                                                                                                             aGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCAt       <  2:856851/150‑1 (MQ=255)
                                                                                                                                                aaaaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATggg    >  1:1899319/1‑150 (MQ=255)
                                                                                                                                                  aaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGTATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCATGCATGGGaa  >  2:5298997/1‑150 (MQ=255)
                                                                                                                                                  aaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGaa  >  1:4915211/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAA  >  CP009273/3828057‑3828352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑