Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,832,308 G→C 24.3% S234T (AGT→ACT)  yicL → EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,832,3080GC24.3% 657.4 / 227.6 291S234T (AGT→ACT) yicLEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (111/100);  new base C (32/38);  total (153/138)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTGT  >  CP009273/3832159‑3832457
                                                                                                                                                     |                                                                                                                                                     
 aCGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGt                                                                                                                                                      <  2:2955229/150‑1 (MQ=255)
  cGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTACTTGGTACGTCCCTGACAATTACTc                                                                                                                                                     >  1:4123841/1‑150 (MQ=255)
  cGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTc                                                                                                                                                     <  1:5138843/150‑1 (MQ=255)
   gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCt                                                                                                                                                    >  2:792497/1‑150 (MQ=255)
   gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCt                                                                                                                                                    <  2:5051054/150‑1 (MQ=255)
   gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCt                                                                                                                                                    <  1:2958109/150‑1 (MQ=255)
    ttACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTg                                                                                                                                                   <  1:4545582/150‑1 (MQ=255)
     tACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGt                                                                                                                                                  <  2:2502678/150‑1 (MQ=255)
      aCCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTa                                                                                                                                                 >  2:1777697/1‑150 (MQ=255)
            cgtcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGaa                                                                                                                                           <  2:3589833/150‑1 (MQ=255)
                     gAGTAGGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGcaca                                                                                                                                  <  1:4252266/150‑1 (MQ=255)
                       gTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTGTTTTTATTTGGTGGTCATTGGTACGTCCCAGACCTTTAGTCTGTACCAGAAATGAGCACaat                                                                                                                                >  1:2693806/1‑150 (MQ=255)
                            cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTAAGTCCCAGACATTTAGTATGTAACTTAAAGTATAACaaataatt                                                                                                                           >  1:3270916/1‑150 (MQ=255)
                              gATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTgg                                                                                                                         <  1:3263864/150‑1 (MQ=255)
                               aTTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGc                                                                                                                        <  2:2390063/150‑1 (MQ=255)
                                    cGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGAGTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTcc                                                                                                                   <  1:5047908/150‑1 (MQ=255)
                                     ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                                  >  2:4273873/1‑150 (MQ=255)
                                           aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACCATTAATTGGCGGTCCAAAAGcc                                                                                                            >  2:1368614/1‑150 (MQ=255)
                                           aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                            >  2:2802192/1‑150 (MQ=255)
                                           aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                            >  1:805805/1‑150 (MQ=255)
                                             tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAg                                                                                                          <  2:61162/150‑1 (MQ=255)
                                                 ttGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAtt                                                                                                      <  1:1024919/150‑1 (MQ=255)
                                                  tGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAttt                                                                                                     <  1:3558390/150‑1 (MQ=255)
                                                   gCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAtttt                                                                                                    <  1:953974/150‑1 (MQ=255)
                                                    ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTg                                                                                                   <  1:3411896/150‑1 (MQ=255)
                                                    ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTg                                                                                                   <  2:68612/150‑1 (MQ=255)
                                                    ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTg                                                                                                   >  1:5266072/1‑150 (MQ=255)
                                                     cTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGa                                                                                                  <  2:668409/150‑1 (MQ=255)
                                                        tttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTACCTGACAATTAGTCTGTAACGGAAAGGAGCACAATTAAATGGCGGTCCAAAATCCCGCAGTTTGAGCt                                                                                               >  1:1110208/1‑150 (MQ=255)
                                                          tATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTGTTTTTATTTGGTGGTCAGTGGTACGTCCCGGGCATTTTCTCTGTACCGGAAAGGAGCACAAATAAATTGCGGTCCAAAAGCCAGCAAGTTGAGCggt                                                                                             >  1:38057/1‑150 (MQ=255)
                                                           aTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                            <  2:1977524/150‑1 (MQ=255)
                                                             gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCa                                                                                          >  2:4644531/1‑150 (MQ=255)
                                                             gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCa                                                                                          <  1:1129757/150‑1 (MQ=255)
                                                              ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTAATTGGTACGGCCCTGAAATTTACTCTTTACCAGAAAGGAGAACAAATAATTGGCGGTCCAAAATCCAGCAGTTTGTGCTGTGGAg                                                                                         >  1:2030829/1‑150 (MQ=255)
                                                              ccAGACAAGGAACAAACTTCGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                         <  1:4686953/150‑1 (MQ=255)
                                                                aGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTGTTTTTTTTTTGTGGTCATTGGTACGTCCCTGACAATTAGTATGTACCTGAAAGGAGCACAAATAATTGGCGGTCCCAAAACCAGCAATTTGAGCTGTGCAGaa                                                                                       >  1:2050489/1‑150 (MQ=255)
                                                                   cAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACCATTAATTGGCGGTCCAAAAGCCCGCATTTTGAGCTGTGCAGCAccc                                                                                    >  2:4814192/1‑149 (MQ=255)
                                                                   cAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAACa                                                                                    >  2:1142769/1‑150 (MQ=255)
                                                                    aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                                   >  2:1271584/1‑150 (MQ=255)
                                                                       gAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCCTTTTGAGCTGTGCAGAACCATTaa                                                                                >  2:3862282/1‑150 (MQ=255)
                                                                       gAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTaa                                                                                <  1:1458189/150‑1 (MQ=255)
                                                                         aCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGt                                                                              <  2:2587827/150‑1 (MQ=255)
                                                                          cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                             >  2:3963138/1‑150 (MQ=255)
                                                                          cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTACAAAAGCACGCATTTTGAGCTGTGAAGAACCATTAAGTa                                                                             >  2:2155153/1‑150 (MQ=255)
                                                                          cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                             <  2:2237454/150‑1 (MQ=255)
                                                                            aaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAgc                                                                           >  2:2123404/1‑150 (MQ=255)
                                                                             aCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAgcg                                                                          >  2:3915975/1‑150 (MQ=255)
                                                                              cTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTAAGTCCCTTACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCATCATTTTGAGCTGTGCAGAACCATTAAGTAgcgc                                                                         >  2:3333543/1‑150 (MQ=255)
                                                                                    cGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAct                                                                   >  2:2541567/1‑150 (MQ=255)
                                                                                     gTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTTGTAAGTCCCTGACATTTAGTATTTAACTGAAAGGATCACAATTAAATTGCGGTCCAAAATCCAGAAATTTGAGCTGTGCAGAACCATTAAGTAGAGCGCTAcac                                                                  >  1:3967507/1‑148 (MQ=255)
                                                                                         aCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCtt                                                              <  2:1502519/150‑1 (MQ=255)
                                                                                         aCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCtt                                                              <  2:2426138/150‑1 (MQ=255)
                                                                                         aCGGCAGTTTGATTCTGGCGTTTTTTTATTAGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCtt                                                              <  2:2662223/150‑1 (MQ=255)
                                                                                                  tGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctgttgc                                                     <  2:2693859/150‑1 (MQ=255)
                                                                                                   gATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCAGACATTTAGTCTGTACCGGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTTAGCTGTGCAGAACCATTAAGTATCGCGCTACTCTCTttgctgttgct                                                    >  2:3343329/1‑150 (MQ=255)
                                                                                                        tGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACAATTACACAGTAACAGAAAGGAGCACAAATAATTGGCGGTCCAAAAACCACCAATTTGAGCTGTTCAGAACCAATAAATATCGCGCGACTCTCTATGCTGTTTCTGGGGa                                               >  1:1574011/1‑150 (MQ=255)
                                                                                                           cGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCa                                            >  2:1598218/1‑150 (MQ=255)
                                                                                                            gTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCAc                                           >  1:4206217/1‑150 (MQ=255)
                                                                                                            gTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCAc                                           <  1:4317142/150‑1 (MQ=255)
                                                                                                            gTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCAc                                           >  2:3127751/1‑150 (MQ=255)
                                                                                                             tttttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACg                                          <  1:2357515/150‑1 (MQ=255)
                                                                                                              ttttttATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGt                                         >  2:4758881/1‑150 (MQ=255)
                                                                                                                 tttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTca                                      >  1:4766872/1‑150 (MQ=255)
                                                                                                                  ttATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcac                                     <  2:4875278/150‑1 (MQ=255)
                                                                                                                    aTTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACAt                                   >  2:3434670/1‑150 (MQ=255)
                                                                                                                      ttGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTa                                 >  1:3171060/1‑150 (MQ=255)
                                                                                                                      ttGGTGGTCATTGGTACGTCCCTGACATTTAGACTGAACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTa                                 >  1:3686365/1‑150 (MQ=255)
                                                                                                                       tggtggTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                                >  2:38320/1‑150 (MQ=255)
                                                                                                                          tggtCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCgg                             <  2:3697816/150‑1 (MQ=255)
                                                                                                                             tCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGAct                          >  1:3050042/1‑150 (MQ=255)
                                                                                                                              cATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActg                         >  2:2824554/1‑150 (MQ=255)
                                                                                                                               aTTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                        >  1:1701777/1‑150 (MQ=255)
                                                                                                                                ttGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggc                       >  1:2821879/1‑150 (MQ=255)
                                                                                                                                 tGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggct                      <  1:1257777/150‑1 (MQ=255)
                                                                                                                                 tGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggct                      <  2:908168/150‑1 (MQ=255)
                                                                                                                                  ggTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggctg                     >  2:3681006/1‑150 (MQ=255)
                                                                                                                                  ggTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggctg                     <  2:1732590/150‑1 (MQ=255)
                                                                                                                                      cGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa                 >  2:3500660/1‑150 (MQ=255)
                                                                                                                                      cGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa                 >  2:423089/1‑150 (MQ=255)
                                                                                                                                       gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc                >  2:5037553/1‑150 (MQ=255)
                                                                                                                                       gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc                <  2:494654/150‑1 (MQ=255)
                                                                                                                                       gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc                >  1:4933221/1‑150 (MQ=255)
                                                                                                                                       gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAGTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc                >  2:1031397/1‑150 (MQ=255)
                                                                                                                                       gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc                <  1:1805207/150‑1 (MQ=255)
                                                                                                                                        tCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACg               <  1:789102/150‑1 (MQ=255)
                                                                                                                                          ccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct             >  2:5129808/1‑150 (MQ=255)
                                                                                                                                           cTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCTCGCTACTCTCTTTTCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGTGAACgctg            >  2:862943/1‑150 (MQ=255)
                                                                                                                                           cTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg            >  2:2840092/1‑150 (MQ=255)
                                                                                                                                           cTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg            >  1:691230/1‑150 (MQ=255)
                                                                                                                                           cTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATCACCGGACTGGCTGGGAACgctg            >  1:3941886/1‑150 (MQ=255)
                                                                                                                                            tGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc           >  1:1331333/1‑150 (MQ=255)
                                                                                                                                             gACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgct          >  2:4548639/1‑150 (MQ=255)
                                                                                                                                             gACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgct          <  1:4644531/150‑1 (MQ=255)
                                                                                                                                             gACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgct          <  1:270025/150‑1 (MQ=255)
                                                                                                                                              aCATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGctgctg         <  2:841324/150‑1 (MQ=255)
                                                                                                                                                aTTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCACCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGAt       >  1:3308482/1‑150 (MQ=255)
                                                                                                                                                 tttAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGAtt      <  1:5112434/150‑1 (MQ=255)
                                                                                                                                                  ttAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTc     >  1:2660398/1‑150 (MQ=255)
                                                                                                                                                    aGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTg   <  1:902279/150‑1 (MQ=255)
                                                                                                                                                    aGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTg   <  1:2559121/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTGT  >  CP009273/3832159‑3832457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑