BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,859,476	G→T	5.8%	T53K (ACA→AAA)	yidE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,859,476	0	G	T	5.8%	994.1 / 20.4	272	T53K (ACA→AAA)	yidE	putative transporter
Reads supporting (aligned to +/- strand): ref base G (159/97); new base T (8/8); total (167/105)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 275 total aligned reads displayed.

TGATGACGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCTATA  >  CP009273/3859327‑3859625

  atgaCGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAGACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTc                                                                                                                                                     >  1:4550997/1‑150 (MQ=255)

    gaCGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCAt                                                                                                                                                   >  1:1005753/1‑150 (MQ=255)

    gaCGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCAt                                                                                                                                                   >  1:2962669/1‑150 (MQ=255)

    gaCGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCAt                                                                                                                                                   <  1:2795026/150‑1 (MQ=255)

          cAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGc                                                                                                                                             >  1:3276736/1‑150 (MQ=255)

           aGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTGAATGTCATCCCCGcc                                                                                                                                            >  2:2097334/1‑150 (MQ=255)

           aGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAAAGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGACTAACAAGCAGCATATCGCTACTAAATGTCAACCCCGcc                                                                                                                                            >  2:1841074/1‑150 (MQ=255)

              aCAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTga                                                                                                                                         >  1:4031094/1‑150 (MQ=255)

              aCAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTga                                                                                                                                         <  2:1298960/150‑1 (MQ=255)

                 gCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGaa                                                                                                                                      >  2:823519/1‑150 (MQ=255)

                    aaCAGGTTGAGGCGTAATGAGGAGACGCGCAATGAGGCATAGTAGCCAGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCATCATATCGCTACTTAATGTCATCACCGCCTTAGAAACa                                                                                                                                   <  2:2383135/150‑1 (MQ=255)

                    aaCAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACa                                                                                                                                   <  2:448236/150‑1 (MQ=255)

                     aCAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACaa                                                                                                                                  <  1:5311393/150‑1 (MQ=255)

                        ggTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAAt                                                                                                                               >  1:2255051/1‑150 (MQ=255)

                          ttGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATgg                                                                                                                             >  2:2333684/1‑150 (MQ=255)

                          ttGAGGCGGACTCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATCAACGATCAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATgg                                                                                                                             <  1:1392667/150‑1 (MQ=255)

                                cGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCcgacg                                                                                                                       >  1:2452108/1‑150 (MQ=255)

                                cGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCcgacg                                                                                                                       >  2:1108856/1‑150 (MQ=255)

                                  tAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACgat                                                                                                                     <  2:1221170/150‑1 (MQ=255)

                                  tAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCAGAGAAACAAAATGGCCGACgat                                                                                                                     >  1:2680750/1‑150 (MQ=255)

                                   aaTCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACACAATGGCCGACgatg                                                                                                                    >  2:4942725/1‑150 (MQ=255)

                                      ccGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATc                                                                                                                 >  1:983923/1‑150 (MQ=255)

                                      ccGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATc                                                                                                                 >  1:2229511/1‑150 (MQ=255)

                                       cGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATcc                                                                                                                >  1:2642056/1‑150 (MQ=255)

                                       cGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATcc                                                                                                                <  2:3225570/150‑1 (MQ=255)

                                        ggAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATccc                                                                                                               >  2:3973256/1‑150 (MQ=255)

                                        ggAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATccc                                                                                                               >  2:4811393/1‑150 (MQ=255)

                                         gagaCGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCcca                                                                                                              <  1:1117100/150‑1 (MQ=255)

                                              gcgcAATGAGGCAAAGAAGCCCGGCCCTTCCTGAATCCCGATAGTATAAACGATCAGGATCAGGCCAAAGTCCTGAATAACATGCAGCTTATCGCTACTTAATGTCATCCCCGCCTGTGAAACAAAATGGCCGACGATGTTCCGACCtaa                                                                                                         <  2:3459866/150‑1 (MQ=255)

                                                gcAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACa                                                                                                       <  2:1260577/150‑1 (MQ=255)

                                                gcAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACa                                                                                                       <  1:2900066/150‑1 (MQ=255)

                                                 cAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAATATGGCCGACGATGATCCCACCAAACAg                                                                                                      >  2:4281533/1‑150 (MQ=255)

                                                 cAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAg                                                                                                      >  1:420027/1‑150 (MQ=255)

                                                 cAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAg                                                                                                      >  2:560325/1‑150 (MQ=255)

                                                  aaTGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGc                                                                                                     >  1:838825/1‑150 (MQ=255)

                                                    tGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCAc                                                                                                   >  1:60216/1‑150 (MQ=255)

                                                       ggCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCAcgcc                                                                                                <  2:472097/150‑1 (MQ=255)

                                                         cAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCAcgccgt                                                                                              <  2:2011604/150‑2 (MQ=255)

                                                          aaaGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACgccgcc                                                                                             >  2:277085/1‑150 (MQ=255)

                                                          aaaGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACgccgcc                                                                                             >  1:5040529/1‑150 (MQ=255)

                                                           aagaagCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCa                                                                                            >  1:1258780/1‑150 (MQ=255)

                                                              aagCCCGGCCCTACCGGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATa                                                                                         >  2:3093590/1‑150 (MQ=255)

                                                                 cccGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCt                                                                                      <  1:3588215/150‑1 (MQ=255)

                                                                    ggCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAAt                                                                                   >  1:4522995/1‑150 (MQ=255)

                                                                    ggCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAAt                                                                                   >  1:4663723/1‑150 (MQ=255)

                                                                    ggCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCAACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAAAACCTaca                                                                                   >  2:3036260/1‑148 (MQ=255)

                                                                        cTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTa                                                                               >  1:1586358/1‑150 (MQ=255)

                                                                          aCCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATg                                                                             >  2:3078841/1‑150 (MQ=255)

                                                                           ccTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGc                                                                            >  1:3474/1‑150 (MQ=255)

                                                                             tGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCcg                                                                          <  2:2743696/150‑1 (MQ=255)

                                                                              gAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCcgc                                                                         >  2:1531335/1‑150 (MQ=255)

                                                                                aTCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGa                                                                       >  1:3359567/1‑150 (MQ=255)

                                                                                 tCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGaa                                                                      >  2:4021016/1‑150 (MQ=255)

                                                                                   ccGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAAt                                                                    <  2:2962669/150‑1 (MQ=255)

                                                                                   ccGAGAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAAt                                                                    <  2:1874157/150‑1 (MQ=255)

                                                                                          tataAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGt                                                             >  1:5218273/1‑150 (MQ=255)

                                                                                           ataAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGtt                                                            <  2:1946613/150‑1 (MQ=255)

                                                                                            taAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTg                                                           >  1:4122264/1‑150 (MQ=255)

                                                                                            taAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTg                                                           <  2:2806125/150‑1 (MQ=255)

                                                                                            taAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTg                                                           <  2:624519/150‑1 (MQ=255)

                                                                                             aaaCGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGc                                                          >  2:2540235/1‑150 (MQ=255)

                                                                                                 gAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGAt                                                      >  1:4972578/1‑150 (MQ=255)

                                                                                                      ggATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATaaaca                                                 >  1:1469020/1‑150 (MQ=255)

                                                                                                      ggATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATaaaca                                                 >  1:2056026/1‑150 (MQ=255)

                                                                                                       gATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATaaacaa                                                >  2:755338/1‑150 (MQ=255)

                                                                                                          cAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAACCAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAAcc                                             <  2:4377036/150‑1 (MQ=255)

                                                                                                          cAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAAcc                                             <  1:64292/150‑1 (MQ=255)

                                                                                                            ggCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACcga                                           >  1:4058926/1‑150 (MQ=255)

                                                                                                                aaaTTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCgacgac                                       <  2:2799514/150‑1 (MQ=255)

                                                                                                                    tCCTGAATAACATGCAGCATATCGCTACTTAATTTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGcc                                   >  2:1104985/1‑150 (MQ=255)

                                                                                                                     ccTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGcca                                  >  1:2519374/1‑150 (MQ=255)

                                                                                                                      cTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGccac                                 >  1:3946991/1‑150 (MQ=255)

                                                                                                                       ttaaTAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACCGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGccacc                                <  1:2362441/148‑1 (MQ=255)

                                                                                                                       tGAATAACATGCAGCATATCGATACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGccacc                                <  1:4611914/150‑1 (MQ=255)

                                                                                                                        taaTAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGccacca                               <  2:4532279/149‑1 (MQ=255)

                                                                                                                            aaCATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGc                           >  1:3132521/1‑150 (MQ=255)

                                                                                                                              cATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCa                         >  2:3386621/1‑150 (MQ=255)

                                                                                                                               aTGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAg                        >  1:28457/1‑150 (MQ=255)

                                                                                                                               aTGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAg                        >  2:5057562/1‑150 (MQ=255)

                                                                                                                                  cagcaTATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAAt                     <  1:3501957/150‑1 (MQ=255)

                                                                                                                                  cagcaTATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAAt                     >  1:1912229/1‑150 (MQ=255)

                                                                                                                                    gcaTATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATAc                   <  1:786670/150‑1 (MQ=255)

                                                                                                                                     caTATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACt                  <  2:316063/150‑1 (MQ=255)

                                                                                                                                     caTATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACt                  <  1:4834799/150‑1 (MQ=255)

                                                                                                                                       tatCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGa                >  1:178227/1‑150 (MQ=255)

                                                                                                                                       tatCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGa                >  1:1334866/1‑150 (MQ=255)

                                                                                                                                        atCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGAc               >  2:3781094/1‑150 (MQ=255)

                                                                                                                                          cGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCg             >  1:1163873/1‑150 (MQ=255)

                                                                                                                                          cGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCg             <  2:1402886/150‑1 (MQ=255)

                                                                                                                                          cGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCg             <  1:437708/150‑1 (MQ=255)

                                                                                                                                            cTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCTAAATTTGACGTTGCCGTTAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGtt           <  2:2255031/150‑1 (MQ=255)

                                                                                                                                              aCTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTaa         >  1:678889/1‑150 (MQ=255)

                                                                                                                                              aCTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACCGACCGTTaa         <  1:2545583/150‑1 (MQ=255)

                                                                                                                                                 tAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGc      <  2:4663723/150‑1 (MQ=255)

                                                                                                                                                 tAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGc      <  1:3823766/150‑1 (MQ=255)

                                                                                                                                                  aaTGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCt     >  1:3092697/1‑150 (MQ=255)

                                                                                                                                                  aaTGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCt     <  2:3347355/150‑1 (MQ=255)

                                                                                                                                                  aaTGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACAGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCt     >  1:173792/1‑150 (MQ=255)

                                                                                                                                                   aTGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCta    <  2:2553412/150‑1 (MQ=255)

                                                                                                                                                     gTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCtata  >  2:5234321/1‑150 (MQ=255)

TGATGACGATCAGAACAGCAAACAGGTTGAGGCGTAATCCGGAGACGCGCAATGAGGCAAAGAAGCCCGGCCCTACCTGAATCCCGATAGTATAAACGAACAGGATCAGGCCAAATTCCTGAATAACATGCAGCATATCGCTACTTAATGTCATCCCCGCCTGAGAAACAAAATGGCCGACGATGATCCCACCAAACAGCACGCCGCCAATACCTAATCCTATGCCGCGAAATTTGACGTTGCCGATAAACAAACCGACGACTGCCACCAAAGCCAGAATACTGACCGTTAATGCTATA  >  CP009273/3859327‑3859625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`