Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,861,553 C→A 100% G373C (GGC→TGC)  yidR ← DUF3748 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,861,5530CA98.6% 934.9 / 2.4 292G373C (GGC→TGC) yidRDUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (159/127);  total (159/133)
Fisher's exact test for biased strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

ATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACA  >  CP009273/3861406‑3861699
                                                                                                                                                   |                                                                                                                                                  
aTGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTcgcagc                                                                                                                                                  <  1:3579394/150‑1 (MQ=255)
 tGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCt                                                                                                                                                 <  1:4569230/150‑1 (MQ=255)
   ccTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                               >  1:1520545/1‑150 (MQ=255)
     tGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgc                                                                                                                                             >  2:1821086/1‑150 (MQ=255)
        tAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCa                                                                                                                                          >  1:1817321/1‑150 (MQ=255)
          aCTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATg                                                                                                                                        >  2:4291373/1‑150 (MQ=255)
           cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATgg                                                                                                                                       >  2:1338175/1‑150 (MQ=255)
           cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATgg                                                                                                                                       <  1:2177421/150‑1 (MQ=255)
            tGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATggg                                                                                                                                      >  1:3244994/1‑150 (MQ=255)
              aTTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGca                                                                                                                                    >  1:4662686/1‑150 (MQ=255)
                 tAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACaa                                                                                                                                 >  2:3340384/1‑150 (MQ=255)
                    cGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                              >  1:2595558/1‑150 (MQ=255)
                       tCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAtt                                                                                                                           >  2:2434821/1‑150 (MQ=255)
                              cGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAt                                                                                                                    <  1:1624932/150‑1 (MQ=255)
                               ggTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATc                                                                                                                   >  1:3070870/1‑150 (MQ=255)
                                gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATcc                                                                                                                  <  2:913174/150‑1 (MQ=255)
                                gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATcc                                                                                                                  >  1:2023144/1‑150 (MQ=255)
                                 tGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                                 <  1:3991231/150‑1 (MQ=255)
                                 tGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACGCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                                 >  2:2848307/1‑150 (MQ=255)
                                  gATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAg                                                                                                                >  1:3370770/1‑150 (MQ=255)
                                  gATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAg                                                                                                                >  2:2601051/1‑150 (MQ=255)
                                   aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGATCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGc                                                                                                               >  1:2501756/1‑150 (MQ=255)
                                    tCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGca                                                                                                              >  2:5241071/1‑150 (MQ=255)
                                     ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcac                                                                                                             <  2:2524110/150‑1 (MQ=255)
                                      cacaGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcaca                                                                                                            >  1:3476530/1‑150 (MQ=255)
                                        caGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACaaa                                                                                                          <  2:4244410/150‑1 (MQ=255)
                                         aGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCACTTCGATTATCCAGCACAAAg                                                                                                         >  2:4791146/1‑150 (MQ=255)
                                         aGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAg                                                                                                         <  1:4393908/150‑1 (MQ=255)
                                                cgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                  >  2:3653358/1‑150 (MQ=255)
                                                   cTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAtt                                                                                               <  2:3408391/150‑1 (MQ=255)
                                                     tccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtct                                                                                             <  2:685910/150‑1 (MQ=255)
                                                       catccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                           <  2:1200026/150‑1 (MQ=255)
                                                       catccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                           <  2:3868081/150‑1 (MQ=255)
                                                       catccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                           <  1:3457702/150‑1 (MQ=255)
                                                       catccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTcc                                                                                           <  2:4689873/150‑1 (MQ=255)
                                                        atccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCt                                                                                          >  1:5208547/1‑150 (MQ=255)
                                                          ccaCGCCAGCCCCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGACTTGGCGTGGTGGTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCACTTCGATTCTCCAGCACAAAGCCCAACCATTCTCCTGa                                                                                        >  2:4585824/1‑150 (MQ=255)
                                                          ccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGa                                                                                        <  2:2676996/150‑1 (MQ=255)
                                                           caCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGAc                                                                                       >  1:2547444/1‑150 (MQ=255)
                                                            aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCNTTCTCCTGACg                                                                                      <  1:2764233/150‑1 (MQ=255)
                                                            aCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACg                                                                                      <  1:2822353/150‑1 (MQ=255)
                                                               ccagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGAt                                                                                   <  2:2135124/150‑1 (MQ=255)
                                                                   ccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                                                               <  1:2517915/150‑1 (MQ=255)
                                                                       tGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTa                                                                           <  1:1873554/150‑1 (MQ=255)
                                                                       tGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTa                                                                           >  2:405476/1‑150 (MQ=255)
                                                                          ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAt                                                                        >  1:3673317/1‑150 (MQ=255)
                                                                          ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAAt                                                                        >  2:1578647/1‑150 (MQ=255)
                                                                           cATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                       <  2:3474170/150‑1 (MQ=255)
                                                                           cATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATg                                                                       <  2:5231325/150‑1 (MQ=255)
                                                                                ggCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGAc                                                                  <  1:4373186/150‑1 (MQ=255)
                                                                                 gCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACt                                                                 <  2:3606472/150‑1 (MQ=255)
                                                                                 gCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACt                                                                 <  2:4409096/150‑1 (MQ=255)
                                                                                   gagaAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               >  1:5259955/1‑150 (MQ=255)
                                                                                   gagaAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               >  2:3467643/1‑150 (MQ=255)
                                                                                   gagaAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                               >  1:4756489/1‑150 (MQ=255)
                                                                                      aagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAata                                                            >  1:2158121/1‑150 (MQ=255)
                                                                                         aCCACGGCGTCCGCAGAAGGTGACTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAGTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATcc                                                         <  2:2880233/150‑1 (MQ=255)
                                                                                         aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATcc                                                         <  1:359032/150‑1 (MQ=255)
                                                                                          ccACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCg                                                        >  1:34486/1‑150 (MQ=255)
                                                                                            aCGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGtt                                                      <  2:3971642/150‑1 (MQ=255)
                                                                                            aCGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAATCGATTATCCAGCACAAAGCCCAACCATTCTCCGGACGGATGCCAGTTAAATGCAGACTGAATCTCCGtt                                                      >  2:3292105/1‑150 (MQ=255)
                                                                                               gCGTCCGCAGATGGTGAATTGGCGTGGTGTTCGGTTATATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACACAGCCCAACCATTCTCCTGACGGTTGCCAGTTAATTGCAGACTGAATATCCGTTTTg                                                   <  2:1786474/150‑1 (MQ=255)
                                                                                                 gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAACGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                                 <  2:622847/150‑1 (MQ=255)
                                                                                                  tCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCCCAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTa                                                >  2:412743/1‑150 (MQ=255)
                                                                                                  tCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTa                                                <  1:2196041/150‑1 (MQ=255)
                                                                                                    cGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATg                                              <  1:1099689/150‑1 (MQ=255)
                                                                                                    cGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATg                                              <  1:50812/150‑1 (MQ=255)
                                                                                                     gCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATgg                                             >  2:544890/1‑150 (MQ=255)
                                                                                                     gCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGGTTTGTGATgg                                             >  1:675598/1‑150 (MQ=255)
                                                                                                      cAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATggg                                            >  2:383102/1‑150 (MQ=255)
                                                                                                       aGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCAGTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGt                                           <  1:4165785/150‑1 (MQ=255)
                                                                                                         aaGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGGGTTGTTATGGGTTa                                         >  2:116769/1‑150 (MQ=255)
                                                                                                          aGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTaa                                        >  1:93080/1‑150 (MQ=255)
                                                                                                          aGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTaa                                        >  1:4888676/1‑150 (MQ=255)
                                                                                                             tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                     <  1:2412408/150‑1 (MQ=255)
                                                                                                             tGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTg                                     <  1:3174187/150‑1 (MQ=255)
                                                                                                                aTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcg                                  >  1:797628/1‑150 (MQ=255)
                                                                                                                aTTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcg                                  >  1:578609/1‑150 (MQ=255)
                                                                                                                aTTGGCGTGGTGTTCGGCTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcg                                  >  2:2371886/1‑150 (MQ=255)
                                                                                                                       tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcg                           >  2:2997344/1‑150 (MQ=255)
                                                                                                                          tgtTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgccg                        <  1:384885/150‑1 (MQ=255)
                                                                                                                              cGGTTAAATCCTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCt                    <  1:3310278/150‑1 (MQ=255)
                                                                                                                                    aaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTGATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCg              >  2:3068120/1‑150 (MQ=255)
                                                                                                                                      tACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag            >  1:1458725/1‑150 (MQ=255)
                                                                                                                                      tACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag            <  2:1835112/150‑1 (MQ=255)
                                                                                                                                      gaCTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGCTTATCCCGCACAAAGCCCACCCATTCTCCTGACGGATGCCAGTTAAATGCAGACGGACTATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag            <  2:2298844/149‑1 (MQ=255)
                                                                                                                                        cTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGAt          <  1:1294880/150‑1 (MQ=255)
                                                                                                                                        cTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGAt          <  1:3773989/150‑1 (MQ=255)
                                                                                                                                         tCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATa         >  2:4942935/1‑150 (MQ=255)
                                                                                                                                         tCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATa         >  2:2760792/1‑150 (MQ=255)
                                                                                                                                          cacccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCATGACGGATGCCAGTTAACTGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATaa        <  2:2344477/147‑1 (MQ=255)
                                                                                                                                           aaCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAg       >  2:4644042/1‑150 (MQ=255)
                                                                                                                                           aaCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAg       >  2:5083696/1‑150 (MQ=255)
                                                                                                                                           aaCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAg       >  2:1989284/1‑150 (MQ=255)
                                                                                                                                            aCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGc      >  2:2507920/1‑150 (MQ=255)
                                                                                                                                             ccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc     >  1:3700441/1‑150 (MQ=255)
                                                                                                                                              cTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCca    <  1:2619439/150‑1 (MQ=255)
                                                                                                                                                cgcagcTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcaca  <  1:501087/150‑1 (MQ=255)
                                                                                                                                                cgcagcTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcaca  >  2:1040932/1‑150 (MQ=255)
                                                                                                                                                cgcagcTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCcaca  <  2:3184637/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCACA  >  CP009273/3861406‑3861699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑