Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,871,587 G→T 28.2% N631K (AAC→AAA)  gyrB ← DNA gyrase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,871,5870GT28.2% 619.8 / 204.4 304N631K (AAC→AAA) gyrBDNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base G (104/114);  new base T (52/34);  total (156/148)
Fisher's exact test for biased strand distribution p-value = 5.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

CTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTT  >  CP009273/3871440‑3871736
                                                                                                                                                   |                                                                                                                                                     
cTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCttt                                                                                                                                                     <  1:49164/150‑1 (MQ=255)
cTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGtt                                                                                                                                                     >  2:2013517/1‑150 (MQ=255)
 tCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTc                                                                                                                                                    <  1:67200/150‑1 (MQ=255)
 tCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGGTc                                                                                                                                                    >  2:4755611/1‑150 (MQ=255)
  cGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCa                                                                                                                                                   >  2:4111116/1‑150 (MQ=255)
     gATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCAccc                                                                                                                                                >  1:3272462/1‑150 (MQ=255)
      aTCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCa                                                                                                                                               <  1:4194049/150‑1 (MQ=255)
       tCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAg                                                                                                                                              >  2:4377084/1‑150 (MQ=255)
              gATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTaa                                                                                                                                       >  2:4707048/1‑150 (MQ=255)
                  gTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGt                                                                                                                                   >  1:2364286/1‑150 (MQ=255)
                   tCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCACTGACCAGCGCTTTCACCCAGCGGGTAACGGTc                                                                                                                                  <  2:3387587/150‑1 (MQ=255)
                     aGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTg                                                                                                                                <  1:1608972/150‑1 (MQ=255)
                     aGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTg                                                                                                                                <  1:74645/150‑1 (MQ=255)
                      gTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGc                                                                                                                               >  1:4720868/1‑150 (MQ=255)
                       tATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCt                                                                                                                              <  1:3063296/150‑1 (MQ=255)
                       tATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACGTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCt                                                                                                                              <  1:3849887/150‑1 (MQ=255)
                        aTCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCtc                                                                                                                             >  2:2495854/1‑150 (MQ=255)
                        aTCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCtc                                                                                                                             >  2:268386/1‑150 (MQ=255)
                          ccACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCtcat                                                                                                                           <  2:2109561/150‑1 (MQ=255)
                           cacaCCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCAAGCGGGTAAAGGTATGCtcatc                                                                                                                          >  1:1816058/1‑150 (MQ=255)
                             cacCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAg                                                                                                                        <  2:775385/150‑1 (MQ=255)
                              acCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGa                                                                                                                       >  2:3880800/1‑150 (MQ=255)
                              acCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGa                                                                                                                       >  1:1900612/1‑150 (MQ=255)
                                cGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCCGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGaaa                                                                                                                     <  1:5301901/150‑1 (MQ=255)
                                 gTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                                                                                    <  2:3720360/150‑1 (MQ=255)
                                 gTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACGTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAg                                                                                                                    <  1:2974810/150‑1 (MQ=255)
                                    ggTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTc                                                                                                                 <  2:3650090/150‑1 (MQ=255)
                                     gTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCa                                                                                                                >  1:2688766/1‑150 (MQ=255)
                                      tACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAg                                                                                                               >  1:1082070/1‑150 (MQ=255)
                                       acgcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGc                                                                                                              >  1:3755495/1‑150 (MQ=255)
                                         gcacgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCtt                                                                                                            <  1:874236/150‑1 (MQ=255)
                                           acgcGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTcc                                                                                                          <  2:4208434/150‑1 (MQ=255)
                                               gAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCa                                                                                                      >  2:4651320/1‑150 (MQ=255)
                                               gAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCa                                                                                                      >  2:2180299/1‑150 (MQ=255)
                                                  cAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACg                                                                                                   <  2:2298519/150‑1 (MQ=255)
                                                   aaTCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                                                                                  >  1:5120369/1‑150 (MQ=255)
                                                   aaTCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                                                                                  >  1:1676116/1‑150 (MQ=255)
                                                   aaTCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGt                                                                                                  >  2:1402667/1‑150 (MQ=255)
                                                     tCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCg                                                                                                <  1:4250613/150‑1 (MQ=255)
                                                     tCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCg                                                                                                <  2:3504787/150‑1 (MQ=255)
                                                      cGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCgg                                                                                               >  1:280860/1‑150 (MQ=255)
                                                      cGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCgg                                                                                               <  2:1954624/150‑1 (MQ=255)
                                                      cGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCgg                                                                                               >  2:2831829/1‑150 (MQ=255)
                                                       ggCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGc                                                                                              >  2:4160339/1‑150 (MQ=255)
                                                        gCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCt                                                                                             <  2:538455/150‑1 (MQ=255)
                                                         cTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTg                                                                                            >  1:1966651/1‑150 (MQ=255)
                                                         cTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCGTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTg                                                                                            >  2:5045985/1‑150 (MQ=255)
                                                            gAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgata                                                                                         >  2:1100419/1‑150 (MQ=255)
                                                             aaCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatag                                                                                        <  1:4110430/150‑1 (MQ=255)
                                                             aaCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatag                                                                                        >  2:3175384/1‑150 (MQ=255)
                                                             aaCAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatag                                                                                        <  2:2197206/150‑1 (MQ=255)
                                                               cAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagat                                                                                      <  1:1505925/150‑1 (MQ=255)
                                                                aGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTgatagata                                                                                     <  1:434088/150‑1 (MQ=255)
                                                                 ggTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                                                                                    <  2:679143/150‑1 (MQ=255)
                                                                 ggTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATaa                                                                                    <  1:4537336/150‑1 (MQ=255)
                                                                  gTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAg                                                                                   <  2:1779620/150‑1 (MQ=255)
                                                                   ttttGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGc                                                                                  <  1:1393657/150‑1 (MQ=255)
                                                                        cTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCttt                                                                             >  1:2661260/1‑150 (MQ=255)
                                                                         tCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTc                                                                            <  1:1410807/150‑1 (MQ=255)
                                                                          cAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCa                                                                           <  2:2364335/150‑1 (MQ=255)
                                                                          cAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCa                                                                           >  1:2168262/1‑150 (MQ=255)
                                                                             cATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa                                                                        >  2:2576038/1‑150 (MQ=255)
                                                                             cATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCa                                                                        >  1:5093584/1‑150 (MQ=255)
                                                                              aTTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAt                                                                       <  2:479592/150‑1 (MQ=255)
                                                                              aTTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCAt                                                                       >  2:1732791/1‑150 (MQ=255)
                                                                                tGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTg                                                                     <  2:1794875/150‑1 (MQ=255)
                                                                                tGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTg                                                                     <  1:4822699/150‑1 (MQ=255)
                                                                                 ggTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGc                                                                    <  1:2487773/150‑1 (MQ=255)
                                                                                  gtgtGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCt                                                                   <  1:3527640/150‑1 (MQ=255)
                                                                                   tgtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCtt                                                                  >  1:3495904/1‑150 (MQ=255)
                                                                                    gtgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCttt                                                                 <  2:2752859/150‑1 (MQ=255)
                                                                                     tgAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTc                                                                <  2:1507001/150‑1 (MQ=255)
                                                                                       aaCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCgg                                                              >  1:1711594/1‑150 (MQ=255)
                                                                                        aCATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGa                                                             <  2:939030/150‑1 (MQ=255)
                                                                                         cATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGAt                                                            <  2:3766197/150‑1 (MQ=255)
                                                                                           tCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATaa                                                          <  1:2632268/150‑1 (MQ=255)
                                                                                             aaaCTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAacg                                                        <  2:1632546/150‑1 (MQ=255)
                                                                                                    cACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTcc                                                 >  1:1012318/1‑150 (MQ=255)
                                                                                                    cACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGAATTGCTTTCGGATAACGACGCTcc                                                 <  2:3604368/150‑1 (MQ=255)
                                                                                                      cTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCAt                                               >  2:2645488/1‑150 (MQ=255)
                                                                                                        ggCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATAc                                             <  2:2145320/150‑1 (MQ=255)
                                                                                                        ggCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATAc                                             <  1:3894788/150‑1 (MQ=255)
                                                                                                        ggCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATAc                                             <  2:2966849/150‑1 (MQ=255)
                                                                                                            gCCGTGGTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGGTCCCCCCGCGGGTAACGGTCTGCTCATCAGAATGGTCTGCTTCAGTCAACGTCGGCTGATAGTTACTCTCTTTCAGCATTGCTTTCGGATAACGACGCTGCATACGAtt                                         <  2:1459039/150‑1 (MQ=255)
                                                                                                            gCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAtt                                         <  1:2134047/150‑1 (MQ=255)
                                                                                                              cGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGa                                       >  2:100786/1‑150 (MQ=255)
                                                                                                                  cTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAt                                   <  1:2495876/150‑1 (MQ=255)
                                                                                                                  cTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCAt                                   <  1:2855137/150‑1 (MQ=255)
                                                                                                                      tCTTTGTCGGTCAGTTCGCTGACCAGCGCTTTCACCCAGCGGGGAACGGTCTGCTCATCAGAAAGGTCAGCGTCCGTCACCGTCGGCTGATAGATAAGCTCTTTCAGCAGTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTc                               <  2:1908768/150‑1 (MQ=255)
                                                                                                                        tttGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTg                             >  1:996608/1‑150 (MQ=255)
                                                                                                                           gTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGt                          >  2:846616/1‑150 (MQ=255)
                                                                                                                               ttCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTcgcg                      >  1:2215909/1‑150 (MQ=255)
                                                                                                                                         tGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTTCTTTAGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAg            >  1:1986308/1‑150 (MQ=255)
                                                                                                                                         tGACCAGCGCTTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAg            >  1:3651089/1‑150 (MQ=255)
                                                                                                                                         tGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAg            >  2:130066/1‑150 (MQ=255)
                                                                                                                                         tGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAg            <  2:4301302/150‑1 (MQ=255)
                                                                                                                                         tGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGAGTGAGCATTTTCTGCGTCGCGTTGTACTCAg            >  1:4458583/1‑150 (MQ=255)
                                                                                                                                           aCCAGCGCGTTCACCCAGCGGGCAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGAt          >  1:4969808/1‑150 (MQ=255)
                                                                                                                                             cAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATAc        <  2:5069202/150‑1 (MQ=255)
                                                                                                                                              aGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATAcc       <  1:2404153/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTCGTGATCCAGCGGATAGTCAGTATCCACACCGTGGGTACGCACGCGAACAATCGGCTCGAACAGGTTTTGCTCAGCATTGGTGTGAACATCAAACTTCCACTGGCTGCCGTGCTGTTCTTTGTCGTTCAGTTCGCTGACCAGCGCGTTCACCCAGCGGGTAACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTT  >  CP009273/3871440‑3871736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑