BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,891,618	A→T	12.5%	I79I (ATA→ATT)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,891,618	0	A	T	12.5%	876.0 / 93.2	304	I79I (ATA→ATT)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand): ref base A (125/141); new base T (16/22); total (141/163)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 307 total aligned reads displayed.

GAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTT  >  CP009273/3891469‑3891767

 agTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAg                                                                                                                                                      >  2:1893173/1‑150 (MQ=255)

         tGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGtt                                                                                                                                              <  2:3910797/150‑1 (MQ=255)

         tGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGtt                                                                                                                                              <  1:436924/150‑1 (MQ=255)

                aacaacAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTg                                                                                                                                       <  1:2156256/150‑1 (MQ=255)

                   aacaGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGt                                                                                                                                    >  2:650153/1‑150 (MQ=255)

                     caGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTat                                                                                                                                  <  2:3318575/150‑1 (MQ=255)

                      aGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTatg                                                                                                                                 >  1:3638210/1‑150 (MQ=255)

                       gACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTatga                                                                                                                                <  2:2616336/150‑1 (MQ=255)

                       gACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTatga                                                                                                                                <  2:4574736/150‑1 (MQ=255)

                       gACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTatga                                                                                                                                <  2:5300986/150‑1 (MQ=255)

                       gACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTatga                                                                                                                                >  2:3752000/1‑150 (MQ=255)

                          gTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGGTGAATTTGAGTAtgatga                                                                                                                             >  2:3679025/1‑150 (MQ=255)

                                ttattaCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGa                                                                                                                       <  1:5078293/150‑1 (MQ=255)

                                 tattaCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGAc                                                                                                                      <  1:3883568/150‑1 (MQ=255)

                                 tattaCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATTATGAAGAGGAc                                                                                                                      >  2:4333746/1‑150 (MQ=255)

                                 tattaCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGAc                                                                                                                      >  1:2597040/1‑150 (MQ=255)

                                     aCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAt                                                                                                                  <  2:2220755/150‑1 (MQ=255)

                                     aCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAt                                                                                                                  <  1:1003136/150‑1 (MQ=255)

                                     aCTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCGGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAt                                                                                                                  <  2:1068474/150‑1 (MQ=255)

                                      cTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAtt                                                                                                                 <  2:2019111/150‑1 (MQ=255)

                                      cTCGGGTCCCTTTTAGTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAtt                                                                                                                 <  1:3904001/150‑1 (MQ=255)

                                       tCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAttt                                                                                                                >  2:1427602/1‑150 (MQ=255)

                                       tCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACAACTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAAttt                                                                                                                >  1:3702609/1‑150 (MQ=255)

                                         gggTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGc                                                                                                              >  2:2657300/1‑150 (MQ=255)

                                           gTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCg                                                                                                            >  2:272400/1‑150 (MQ=255)

                                               cTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTAt                                                                                                        >  1:3616592/1‑150 (MQ=255)

                                               cTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTAt                                                                                                        >  1:2343113/1‑150 (MQ=255)

                                                ttttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTAtt                                                                                                       <  1:3406785/150‑1 (MQ=255)

                                                ttttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTAtt                                                                                                       <  2:3453785/150‑1 (MQ=255)

                                                 tttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTa                                                                                                      >  1:682214/1‑150 (MQ=255)

                                                 tttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTa                                                                                                      <  1:247442/150‑1 (MQ=255)

                                                 tttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATNTGCCGGTATTa                                                                                                      <  1:2765638/150‑1 (MQ=255)

                                                  ttATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTaa                                                                                                     >  1:2861297/1‑150 (MQ=255)

                                                    aTTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGa                                                                                                   <  2:925308/150‑1 (MQ=255)

                                                    aTTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGa                                                                                                   >  1:3093968/1‑150 (MQ=255)

                                                     ttGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGaa                                                                                                  >  2:5248954/1‑150 (MQ=255)

                                                      tGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAac                                                                                                 <  1:4524697/150‑1 (MQ=255)

                                                      tGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAac                                                                                                 >  1:4218168/1‑150 (MQ=255)

                                                      tGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAac                                                                                                 <  2:5082945/150‑1 (MQ=255)

                                                      tGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAac                                                                                                 <  2:3867971/150‑1 (MQ=255)

                                                      tGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAac                                                                                                 <  1:1303577/150‑1 (MQ=255)

                                                      agCGATGATGTAATTGAACATCTCTGTCCGTGGTGTATTGCGGACTCTTCATCTGATTAAAAGTTTGCAGGTAGTATTCAGGATGATTACATCATATTAGGTGTTGAATTTGAGAATTATGAAGAGGTCGAATTAGGAGATATtaataaa                                                                                                 >  2:4764864/2‑149 (MQ=255)

                                                       gCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAaca                                                                                                <  1:2247822/150‑1 (MQ=255)

                                                       gCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAaca                                                                                                <  1:650153/150‑1 (MQ=255)

                                                         gTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGTCGGTATTAAGAAcaca                                                                                              <  2:1591021/150‑1 (MQ=255)

                                                         gTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAAcaca                                                                                              >  2:4464942/1‑150 (MQ=255)

                                                              tgaAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATcc                                                                                         >  2:3975907/1‑150 (MQ=255)

                                                               gaAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCt                                                                                        <  2:5154826/150‑1 (MQ=255)

                                                                  gTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCAGAGAAGGTGTGGAATTTGAGTATGAGGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCtgat                                                                                     >  2:3647492/1‑150 (MQ=255)

                                                                    tGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCtgatga                                                                                   >  1:1778220/1‑150 (MQ=255)

                                                                     gAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGaa                                                                                  >  1:5164538/1‑150 (MQ=255)

                                                                           ctctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTg                                                                            >  2:4267713/1‑150 (MQ=255)

                                                                            tctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGa                                                                           <  1:1164849/150‑1 (MQ=255)

                                                                            tctGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGa                                                                           <  1:5220637/150‑1 (MQ=255)

                                                                              tGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGaaa                                                                         >  2:3469646/1‑150 (MQ=255)

                                                                               gTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGACGGTATTAAGAACACATATCCTGATGAAATGCTGAAag                                                                        >  1:4121180/1‑150 (MQ=255)

                                                                                    tggtgTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttg                                                                   <  1:3767544/150‑1 (MQ=255)

                                                                                     ggtgTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttgg                                                                  <  2:229529/150‑1 (MQ=255)

                                                                                     ggtgTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttgg                                                                  >  2:792221/1‑150 (MQ=255)

                                                                                      gtgtATTGCGGACGGTTCTGCTGCTGAAAAGTTTTCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggt                                                                 >  1:301603/1‑150 (MQ=255)

                                                                                       tgtATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggtt                                                                >  2:4510121/1‑150 (MQ=255)

                                                                                        gtATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggttg                                                               <  1:4303633/150‑1 (MQ=255)

                                                                                         tATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGa                                                              >  2:3941636/1‑150 (MQ=255)

                                                                                               ggACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAacgcac                                                        >  2:4474095/1‑150 (MQ=255)

                                                                                                gACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAacgcacg                                                       <  1:2716702/150‑1 (MQ=255)

                                                                                                  cGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGcc                                                     >  2:3114032/1‑150 (MQ=255)

                                                                                                   ggTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCa                                                    <  1:2058547/150‑1 (MQ=255)

                                                                                                   ggTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCa                                                    >  2:2975366/1‑150 (MQ=255)

                                                                                                    gTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAg                                                   <  2:3801356/150‑1 (MQ=255)

                                                                                                       ctgctgctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGtt                                                >  2:85686/1‑150 (MQ=255)

                                                                                                            tctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCat                                           <  2:382873/149‑1 (MQ=255)

                                                                                                            gctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCat                                           <  2:4831345/150‑1 (MQ=255)

                                                                                                            gctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCat                                           <  2:4120987/150‑1 (MQ=255)

                                                                                                             ctgAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatg                                          <  2:1189396/150‑1 (MQ=255)

                                                                                                                  aaGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatggatgg                                     >  2:228516/1‑150 (MQ=255)

                                                                                                                  aaGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCatggatgg                                     >  1:1458949/1‑150 (MQ=255)

                                                                                                                   aGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGgc                                    <  2:5276106/150‑1 (MQ=255)

                                                                                                                       tGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGgcagca                                <  1:908805/150‑1 (MQ=255)

                                                                                                                          aGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGa                             >  2:5141581/1‑150 (MQ=255)

                                                                                                                          aGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGa                             >  2:5049085/1‑150 (MQ=255)

                                                                                                                             tAGTTTTCTGGATGATGCCAGCATAGATGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGTAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                          <  1:1111552/150‑1 (MQ=255)

                                                                                                                              aGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTc                         >  1:838373/1‑150 (MQ=255)

                                                                                                                               gTTTTCAGGATGATGCCAGCATTGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCt                        <  2:1449426/150‑1 (MQ=255)

                                                                                                                               gTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCt                        <  2:682214/150‑1 (MQ=255)

                                                                                                                                 tttCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTgg                      <  1:4773096/150‑1 (MQ=255)

                                                                                                                                    cAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTc                   <  2:4089046/150‑1 (MQ=255)

                                                                                                                                    cAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTc                   >  2:2489985/1‑150 (MQ=255)

                                                                                                                                    cAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTc                   <  2:1033397/150‑1 (MQ=255)

                                                                                                                                     aGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcg                  >  2:2840791/1‑150 (MQ=255)

                                                                                                                                     aGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcg                  <  1:4009126/150‑1 (MQ=255)

                                                                                                                                      ggATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTcgc                 >  1:3324621/1‑150 (MQ=255)

                                                                                                                                         tgatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCa              >  2:1725726/1‑150 (MQ=255)

                                                                                                                                         tgatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCa              >  2:1968176/1‑150 (MQ=255)

                                                                                                                                          gatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATGTGCCGGTATTAAGAACACATAGCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAt             <  2:1498457/150‑1 (MQ=255)

                                                                                                                                           atgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCAtt            >  2:466037/1‑150 (MQ=255)

                                                                                                                                               cAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTgg        <  1:2213583/150‑1 (MQ=255)

                                                                                                                                                aGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGc       >  1:943695/1‑150 (MQ=255)

                                                                                                                                                 gCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCg      <  1:4793023/150‑1 (MQ=255)

                                                                                                                                                     aGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGAttt  <  2:4638063/150‑1 (MQ=255)

                                                                                                                                                     aGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGAttt  <  1:1893173/150‑1 (MQ=255)

GAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTT  >  CP009273/3891469‑3891767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`