Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,901,415 C→A 100% P104P (CCG→CCT)  pstB ← phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,901,4150CA98.9% 863.6 / 0.1 265P104P (CCG→CCT) pstBphosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (143/118);  total (143/122)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 265 total aligned reads displayed.

AGAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA  >  CP009273/3901268‑3901561
                                                                                                                                                   |                                                                                                                                                  
agagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                  >  2:2767713/1‑150 (MQ=255)
agagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                  >  2:3841966/1‑150 (MQ=255)
agagTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAgg                                                                                                                                                  <  2:1249129/150‑1 (MQ=255)
   gTAACCGCTCTGGTGCAATTTATCTTTGGTATCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGaaa                                                                                                                                               >  1:1393445/1‑150 (MQ=255)
    tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                              >  1:4501445/1‑150 (MQ=255)
    tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                              <  1:1486103/150‑1 (MQ=255)
    tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                              <  2:3133672/150‑1 (MQ=255)
    tAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAAc                                                                                                                                              <  2:3276992/150‑1 (MQ=255)
     aaCCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACg                                                                                                                                             <  2:3952283/150‑1 (MQ=255)
         gCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGt                                                                                                                                         <  1:3221309/150‑1 (MQ=255)
          ctctGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTc                                                                                                                                        >  1:5286118/1‑150 (MQ=255)
                 gCATTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCt                                                                                                                                 <  2:2220008/150‑1 (MQ=255)
                   aaTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTgg                                                                                                                               >  1:354613/1‑150 (MQ=255)
                   aaTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTgg                                                                                                                               >  1:1349722/1‑150 (MQ=255)
                    aTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGa                                                                                                                              <  1:4970168/150‑1 (MQ=255)
                    aTTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGa                                                                                                                              >  2:1951672/1‑150 (MQ=255)
                      ttATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAac                                                                                                                            >  1:2612637/1‑150 (MQ=255)
                           tttGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCAt                                                                                                                       <  2:249203/150‑1 (MQ=255)
                             tGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGc                                                                                                                     >  1:2869293/1‑150 (MQ=255)
                              ggTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGcc                                                                                                                    >  2:2165089/1‑150 (MQ=255)
                              ggTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGcc                                                                                                                    <  1:4728389/150‑1 (MQ=255)
                              ggTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGcc                                                                                                                    >  1:3072166/1‑150 (MQ=255)
                                 ttCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCAc                                                                                                                 >  2:3473670/1‑150 (MQ=255)
                                   cGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACtt                                                                                                               >  2:3017917/1‑150 (MQ=255)
                                    gTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                              >  1:2343724/1‑150 (MQ=255)
                                    gTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACttt                                                                                                              <  2:1134226/150‑1 (MQ=255)
                                     ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTc                                                                                                             <  1:4912135/150‑1 (MQ=255)
                                     ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTc                                                                                                             >  2:3075527/1‑150 (MQ=255)
                                     ttCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTc                                                                                                             >  1:1615163/1‑150 (MQ=255)
                                       ccACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgc                                                                                                           >  2:4608163/1‑150 (MQ=255)
                                       ccACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgc                                                                                                           <  1:2434404/150‑1 (MQ=255)
                                          caATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacg                                                                                                        <  2:5197756/150‑1 (MQ=255)
                                           aaTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGACACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacgc                                                                                                       >  2:1864958/1‑150 (MQ=255)
                                           aaTGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTcgcacgc                                                                                                       >  1:709070/1‑150 (MQ=255)
                                              gCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACgcagc                                                                                                    <  2:1102837/150‑1 (MQ=255)
                                                ggCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGcagcag                                                                                                  >  1:2153311/1‑150 (MQ=255)
                                                ggCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGcagcag                                                                                                  >  2:2741964/1‑150 (MQ=255)
                                                 gCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGt                                                                                                 >  2:136513/1‑150 (MQ=255)
                                                  cTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTg                                                                                                >  2:198272/1‑150 (MQ=255)
                                                  cTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTg                                                                                                <  2:612065/150‑1 (MQ=255)
                                                  cTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTg                                                                                                <  2:5107708/150‑1 (MQ=255)
                                                    ttGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCg                                                                                              >  2:4745683/1‑150 (MQ=255)
                                                       gTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGata                                                                                           >  1:619738/1‑150 (MQ=255)
                                                        tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                          <  1:1357268/150‑1 (MQ=255)
                                                        tCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGatat                                                                                          >  1:1533047/1‑150 (MQ=255)
                                                         cAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATc                                                                                         >  1:1957076/1‑150 (MQ=255)
                                                          aaTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATcc                                                                                        >  1:4161470/1‑150 (MQ=255)
                                                          aaTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATcc                                                                                        <  1:5104755/150‑1 (MQ=255)
                                                           aTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCt                                                                                       <  2:2336790/150‑1 (MQ=255)
                                                           aTGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCt                                                                                       <  2:3178835/150‑1 (MQ=255)
                                                            tGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTg                                                                                      <  2:2769103/150‑1 (MQ=255)
                                                              cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgag                                                                                    <  1:4558008/150‑1 (MQ=255)
                                                              cccACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTgag                                                                                    >  2:1478843/1‑150 (MQ=255)
                                                                    gCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTgg                                                                              >  1:863983/1‑150 (MQ=255)
                                                                      aCGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTc                                                                            <  1:3667520/150‑1 (MQ=255)
                                                                         cgcTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAgg                                                                         <  1:4280477/150‑1 (MQ=255)
                                                                           cTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAt                                                                       <  2:378168/150‑1 (MQ=255)
                                                                             cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                     >  1:2950476/1‑150 (MQ=255)
                                                                             cGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgt                                                                     >  1:1813267/1‑150 (MQ=255)
                                                                              gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATCTCCTGAGAGTTGGTCAGGAtgtt                                                                    >  2:2098734/1‑150 (MQ=255)
                                                                              gTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGAtgtt                                                                    <  2:600174/150‑1 (MQ=255)
                                                                                ccATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCATAAGCGATGTTGTCGTAGATGGACATAGGAAACGTCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGAGATATCCTGAGAGTTGGTCAGGAtgttgt                                                                  <  2:2610848/150‑1 (MQ=255)
                                                                                 cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTc                                                                 <  2:3570210/150‑1 (MQ=255)
                                                                                 cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTc                                                                 <  2:926418/150‑1 (MQ=255)
                                                                                 cATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTc                                                                 <  1:3819788/150‑1 (MQ=255)
                                                                                        gCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCa                                                          <  2:4272646/150‑1 (MQ=255)
                                                                                         cACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGTCATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCaa                                                         <  2:5126477/150‑1 (MQ=255)
                                                                                            gggAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTNGCCATCAAGCa                                                      <  1:2766153/150‑1 (MQ=255)
                                                                                            gggAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCa                                                      >  1:3295317/1‑150 (MQ=255)
                                                                                              gagaGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGa                                                    >  2:2124266/1‑150 (MQ=255)
                                                                                                gagCTTCTCACACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAt                                                  <  2:1751639/150‑1 (MQ=255)
                                                                                                gagCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAt                                                  <  2:292087/150‑1 (MQ=255)
                                                                                                 agCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAtt                                                 <  1:2210296/150‑1 (MQ=255)
                                                                                                     tctcAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCAc                                             >  1:1759733/1‑150 (MQ=255)
                                                                                                        cAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtt                                          >  2:5016289/1‑150 (MQ=255)
                                                                                                        cAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCtc                                          <  2:2051636/150‑2 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                         >  1:3917073/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                         <  2:2500816/150‑1 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTc                                         >  1:1178160/1‑150 (MQ=255)
                                                                                                         aaaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTACTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGACAGGAGGTTGACGCCAACAAGCAGAAATTCACCTTc                                         >  1:4434738/1‑150 (MQ=255)
                                                                                                          aaCAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTcc                                        <  2:4161470/150‑1 (MQ=255)
                                                                                                            cAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGc                                      >  1:4721295/1‑150 (MQ=255)
                                                                                                              gACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCAc                                    <  1:3147222/150‑1 (MQ=255)
                                                                                                                cGAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgc                                  >  1:471153/1‑150 (MQ=255)
                                                                                                                 gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                 <  1:2703746/150‑1 (MQ=255)
                                                                                                                 gAACGCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACgct                                 >  2:3711406/1‑150 (MQ=255)
                                                                                                                     gCCAAAAGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTc                             <  1:5048147/150‑1 (MQ=255)
                                                                                                                        aaaaGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCgg                          >  2:3671578/1‑150 (MQ=255)
                                                                                                                        aaaaGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCgg                          >  2:1579118/1‑150 (MQ=255)
                                                                                                                          aaGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGt                        >  1:3913679/1‑150 (MQ=255)
                                                                                                                           aGCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTa                       >  2:4874415/1‑150 (MQ=255)
                                                                                                                            gCGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAAATTCACCTTCCGCACGCTGCTCCGGGTAc                      >  1:2133407/1‑150 (MQ=255)
                                                                                                                             cGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                     <  2:1450841/150‑1 (MQ=255)
                                                                                                                             cGATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACa                     >  1:1098156/1‑150 (MQ=255)
                                                                                                                              gATGTTGTCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAg                    <  1:3381072/150‑1 (MQ=255)
                                                                                                                                   tgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa               >  2:4338261/1‑150 (MQ=255)
                                                                                                                                   tgtCGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCaa               <  1:3684451/150‑1 (MQ=255)
                                                                                                                                      cGTAGATGGACATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACa            >  1:2104825/1‑150 (MQ=255)
                                                                                                                                              gACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTttgttg    <  1:4178175/150‑1 (MQ=255)
                                                                                                                                                cATAGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGaa  >  1:4036443/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGAGTAACCGCTCTGGTGCAATTTATCTTTGGTTTCGTTCCACAATGCGGCTTTGGTCAATGCCCACTGCACGCGCTCGTCCATGTCGGCACGGGAGAGCTTCTCAAACAGACGAACGCCAAAAGCGATGTTGTCGTAGATGGACATCGGAAACGGCGTCGGTTTCTGGAACACCATGCCCACTTTCGCACGCAGCAGTGCGATATCCTGAGAGTTGGTCAGGATGTTGTCGCCATCAAGCAGAATTTCACCTTCCGCACGCTGCTCCGGGTACAGTTCAAACATTTTGTTGAA  >  CP009273/3901268‑3901561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑