Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,906,384 G→T 100% I215I (ATC→ATA)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,906,3840GT99.6% 851.6 / ‑4.5 267I215I (ATC→ATA) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (173/92);  total (174/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

GATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGG  >  CP009273/3906237‑3906530
                                                                                                                                                   |                                                                                                                                                  
  tCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAttt                                                                                                                                                <  2:313566/150‑1 (MQ=255)
   cgcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTc                                                                                                                                               <  1:4622211/150‑1 (MQ=255)
      gTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGc                                                                                                                                            >  1:4879042/1‑150 (MQ=255)
          ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                        <  1:3728517/150‑1 (MQ=255)
          ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                        >  2:5159673/1‑150 (MQ=255)
          ggCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                        >  2:4391828/1‑150 (MQ=255)
           gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                       <  2:903757/150‑1 (MQ=255)
           gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                       <  1:849442/150‑1 (MQ=255)
           gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                       >  1:2945612/1‑150 (MQ=255)
           gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                       <  2:5249865/150‑1 (MQ=255)
           gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCATTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTACGAGCCAGTTTTATCGAATATGTTTACCGAACGGCGAGTTATTTAAGCAatat                                                                                                                                       >  2:4544103/1‑150 (MQ=255)
             tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCg                                                                                                                                     <  1:1635560/150‑1 (MQ=255)
             tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCg                                                                                                                                     >  2:4069737/1‑150 (MQ=255)
               ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGcc                                                                                                                                   <  1:1511519/150‑1 (MQ=255)
               ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGcc                                                                                                                                   <  1:1731371/150‑1 (MQ=255)
                tCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGccc                                                                                                                                  <  1:1996913/150‑1 (MQ=255)
                  gTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                >  2:383088/1‑150 (MQ=255)
                  gTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                <  2:4860506/150‑1 (MQ=255)
                    aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                              >  2:1255838/1‑150 (MQ=255)
                     gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                             >  1:1154082/1‑150 (MQ=255)
                     gATATCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                             >  2:3649317/1‑150 (MQ=255)
                          ctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGa                                                                                                                        >  2:2163710/1‑150 (MQ=255)
                            ttCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAg                                                                                                                      <  1:581239/150‑1 (MQ=255)
                             tCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGa                                                                                                                     <  2:1879493/150‑1 (MQ=255)
                             tCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGa                                                                                                                     <  1:1018557/150‑1 (MQ=255)
                              cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                                                                                    >  2:4719827/1‑150 (MQ=255)
                              cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                                                                                    <  1:4375214/150‑1 (MQ=255)
                              cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                                                                                    >  2:3766839/1‑150 (MQ=255)
                                gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa                                                                                                                  <  1:4314192/150‑1 (MQ=255)
                                    gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCg                                                                                                              >  1:5208932/1‑150 (MQ=255)
                                       gTGACGGAAAATGCCTTGATCGCCCGCGTCATATTGCAGATTGTATTCGATCTCCTGACGTTTTACTTCCGCGCCTGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAACGCGACg                                                                                                           <  2:4035456/150‑1 (MQ=255)
                                         gACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACggg                                                                                                         >  2:1044269/1‑150 (MQ=255)
                                          aCGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGt                                                                                                        <  2:3431070/150‑1 (MQ=255)
                                           cGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTc                                                                                                       >  1:408616/1‑150 (MQ=255)
                                            ggTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCa                                                                                                      >  2:2442477/1‑150 (MQ=255)
                                              tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCAcc                                                                                                    >  1:1361087/1‑150 (MQ=255)
                                               aaaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCg                                                                                                   >  2:1470946/1‑150 (MQ=255)
                                               aaaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCg                                                                                                   >  1:948914/1‑150 (MQ=255)
                                                aaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCgg                                                                                                  >  1:2200192/1‑150 (MQ=255)
                                                 ttGCTTTTATCGCCCGCTTCATTTTGCTTTTTGTATTCTATATCCTGACGTTTTACTTCCGCGCCTGTTTTATCGAAGATGTTTACCGAACGGCGATTTATTTCCGCAATATCGCCCTCTTCATGGAAGATAAAGCGACGGGTCACCGGc                                                                                                 <  2:533049/149‑1 (MQ=255)
                                                 aTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGc                                                                                                 >  2:2068938/1‑150 (MQ=255)
                                                    ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAAc                                                                                              >  2:4244193/1‑150 (MQ=255)
                                                    ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAAc                                                                                              >  2:4895607/1‑150 (MQ=255)
                                                     cttaaccgcccgccCAATTTTGCTGTTTGGATACGATATCCTGCCTTTTTAGTCCCGCCCCTGTTTTCCCGAAGTTGTTTCCCTACGGGCTCGTTATTTCCGCCAACGCGCCCAATACACGCAAGAAAACGCGACGGGTCACCGGCAACa                                                                                             <  2:2337483/144‑1 (MQ=255)
                                                         aTCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgcgc                                                                                         >  1:4317004/1‑150 (MQ=255)
                                                          tCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGcc                                                                                        >  1:2391148/1‑150 (MQ=255)
                                                             cccGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGc                                                                                     >  2:755166/1‑150 (MQ=255)
                                                               cgcgTCATATTGCAGTTTGGATTCGATATCCTGACGTTTTCCTTCCGCGCCAGTTTTATCGACGATGTTTCCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCATGGAAGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTg                                                                                   <  2:131760/150‑1 (MQ=255)
                                                               cgcgTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTg                                                                                   >  1:5134624/1‑150 (MQ=255)
                                                                 cgTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGt                                                                                 >  2:2518613/1‑150 (MQ=255)
                                                                     atatTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                             <  2:416802/150‑1 (MQ=255)
                                                                     atatTGAAGATTGTATTCGATATCCTGACTTTTTACTTCCGCGCCTGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCACGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                             <  2:3332232/150‑1 (MQ=255)
                                                                       atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAg                                                                           >  2:2252011/1‑150 (MQ=255)
                                                                       atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAg                                                                           >  2:1928353/1‑150 (MQ=255)
                                                                          gCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGa                                                                        <  2:4879064/150‑1 (MQ=255)
                                                                            aGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATa                                                                      >  1:4392819/1‑150 (MQ=255)
                                                                            aGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGGTATTTCCGCAATATCGCCCTCATCAAGGAAGATAAATCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATa                                                                      >  2:2652827/1‑150 (MQ=255)
                                                                             gATTGGATTCGATATCCTGACGTGTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaa                                                                     >  1:5030422/1‑150 (MQ=255)
                                                                               ttGTATTCGATATCCTTACTTTTTACTTCCGCGCCTGTTTTATCGAAGTTGTTTACCGAAGGGCGTGTTTTTTCCGAAATATCGCCCTCTTCATGGATTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAg                                                                   <  1:421136/150‑1 (MQ=255)
                                                                                tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGt                                                                  >  1:1043426/1‑150 (MQ=255)
                                                                                  taTTCTATATCGTGACTTTTTACTCCCGCGCCTTTTTTATCTATTTTTTTAACCTACGGGCTTTTTTTTCCCGAAATATCGCCCTCTTCATGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                                <  1:2821100/149‑1 (MQ=255)
                                                                                  gATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                                >  1:3685184/1‑150 (MQ=255)
                                                                                     tctatatCCTGACTTTTTACTTCCGCGCCTTTTTTATCGATGTTGTTTCCCGACCGGCGTGTTATTTCCCCAATATCGCCCTCTAAACGGAAGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCg                                                             <  1:341889/147‑1 (MQ=255)
                                                                                     tCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCg                                                             >  1:1294229/1‑150 (MQ=255)
                                                                                       gATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGcc                                                           >  1:182166/1‑150 (MQ=255)
                                                                                         tatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCa                                                         >  1:1116936/1‑150 (MQ=255)
                                                                                           tCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATc                                                       >  2:1824589/1‑150 (MQ=255)
                                                                                            ccTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcc                                                      >  1:121512/1‑150 (MQ=255)
                                                                                             cTGACGTTTTACTCCCGCGCCAGTTTTATCGAAGATGTTTACCGACCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc                                                     <  2:3008711/150‑1 (MQ=255)
                                                                                              tGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc                                                    >  1:2006543/1‑150 (MQ=255)
                                                                                               gACGTTTTACTTCCGCGCCATTTTTATCTAATATGTTTACCTAACGGCGAGTTATTTCCGCAATATCGCCCTCTACAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa                                                   <  2:3737135/150‑1 (MQ=255)
                                                                                               gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa                                                   >  2:4433478/1‑150 (MQ=255)
                                                                                               gACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa                                                   >  2:1641045/1‑150 (MQ=255)
                                                                                                 cGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAgg                                                 >  1:320904/1‑150 (MQ=255)
                                                                                                  tttttACTTCCTCGACTTTTTTATCTAATTTTTTTACCGATGGTCTATTTATTACCGAAATATCGCCCTATTAATGTAATATAACGCGACGGGTCAACTTCAACAGCGCCAGCTGGTCAGAAGCTATAAAGTTTTCGCCCATCCCCAGGc                                                <  2:2033870/149‑1 (MQ=255)
                                                                                                  gTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGc                                                >  1:394529/1‑150 (MQ=255)
                                                                                                  gTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGc                                                >  1:75202/1‑150 (MQ=255)
                                                                                                   ttttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGcc                                               >  1:1253561/1‑150 (MQ=255)
                                                                                                   ttttACTCCCGCGCCTTTTTTATCTATTTTGTTTACCGATCGGCTTTTTTTTTCCGCAATATCGCCCTCTTATTGTATTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGcc                                               <  1:101598/150‑1 (MQ=255)
                                                                                                    tttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCa                                              >  2:3202204/1‑150 (MQ=255)
                                                                                                        cTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCCAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCCGCTGGTCAGAAGCGATCAAGGTTTCGCCCATCCCCAGGCCAATCa                                          >  2:3936061/1‑150 (MQ=255)
                                                                                                        cTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                          >  2:3392534/1‑150 (MQ=255)
                                                                                                              cgcCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCg                                    >  2:674731/1‑150 (MQ=255)
                                                                                                               gcCTGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCgg                                   <  2:1711777/150‑1 (MQ=255)
                                                                                                                  ttttttATCTATGATTTTTCCCGAACGGCTTGTTATTACCGAAATATCGCCCTCTTAAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                <  1:4442048/148‑1 (MQ=255)
                                                                                                                  aGTTTTATCTAAGATTTTTACCTAACGGCGAGTTATTTCCGCAATATCGCCCTATTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                <  2:4344055/150‑1 (MQ=255)
                                                                                                                  aGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                >  2:2691264/1‑150 (MQ=255)
                                                                                                                  aGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                >  2:2809180/1‑150 (MQ=255)
                                                                                                                      ttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCa                            >  1:3016978/1‑150 (MQ=255)
                                                                                                                       tATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAg                           >  1:4814554/1‑150 (MQ=255)
                                                                                                                         tCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGaa                         <  2:879584/150‑1 (MQ=255)
                                                                                                                          cGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCTATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAAc                        >  2:3720574/1‑150 (MQ=255)
                                                                                                                            aaGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGt                      >  1:3993281/1‑150 (MQ=255)
                                                                                                                               atttttACCGAACGGCTTTTTATTTCCGCAATATCGCCCTCTTCAATTAAAAAAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                   <  2:2413158/147‑1 (MQ=255)
                                                                                                                                tttttACCGACCGGCTATTAATTCCCGAAATATCGCCCTCTTCAAGCAAGAAAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg                  <  1:2558083/148‑1 (MQ=255)
                                                                                                                                tGTTTACCGACCGGCGAGTTATTTCCGAAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg                  <  2:3796705/150‑1 (MQ=255)
                                                                                                                                            ggCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt      >  2:560520/1‑150 (MQ=255)
                                                                                                                                              cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc    >  1:356181/1‑150 (MQ=255)
                                                                                                                                               gAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCg   >  2:4618527/1‑150 (MQ=255)
                                                                                                                                                aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCgg  >  2:4444001/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGG  >  CP009273/3906237‑3906530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑