Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,448 G→T 100% V96V (GTC→GTA)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4480GT98.9% 895.2 / 0.0 279V96V (GTC→GTA) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (136/140);  total (137/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 279 total aligned reads displayed.

TTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCC  >  CP009273/3910301‑3910594
                                                                                                                                                   |                                                                                                                                                  
 ttACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTAcc                                                                                                                                                 >  2:4791836/1‑150 (MQ=255)
      gCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTc                                                                                                                                            >  1:3508436/1‑150 (MQ=255)
          ttAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCAccc                                                                                                                                        <  2:3442325/150‑1 (MQ=255)
           tAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCa                                                                                                                                       <  1:2921074/150‑1 (MQ=255)
            aGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                                                      >  2:5106903/1‑150 (MQ=255)
             gCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGt                                                                                                                                     <  2:3747824/150‑1 (MQ=255)
              cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                                                    <  1:612486/150‑1 (MQ=255)
              cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTa                                                                                                                                    >  1:169447/1‑150 (MQ=255)
               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                                                   >  1:930798/1‑150 (MQ=255)
               gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTAc                                                                                                                                   <  2:3769280/150‑1 (MQ=255)
                   ggACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTc                                                                                                                               >  2:5007997/1‑150 (MQ=255)
                    gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                                                              <  2:2712645/150‑1 (MQ=255)
                    gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                                                              >  1:1468626/1‑150 (MQ=255)
                    gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                                                              >  1:5044814/1‑150 (MQ=255)
                     acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCAt                                                                                                                             >  1:2088178/1‑150 (MQ=255)
                     acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCAt                                                                                                                             <  2:2295041/150‑1 (MQ=255)
                      cacaTCATGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                                                            <  2:4078254/150‑1 (MQ=255)
                      cacaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATg                                                                                                                            >  1:2249207/1‑150 (MQ=255)
                       acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGa                                                                                                                           <  1:1958464/150‑1 (MQ=255)
                        caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGAt                                                                                                                          >  1:5174271/1‑150 (MQ=255)
                        caTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGAt                                                                                                                          >  2:1310129/1‑150 (MQ=255)
                           cAGGTCGATAACTTTGATACAGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCGTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCTCCGATCTCGCCTTTCATGTCTACGGGTTCTCGCAGTTCGTTCTTGATACg                                                                                                                       <  1:3770079/150‑1 (MQ=255)
                               tCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGccc                                                                                                                   <  1:1336186/150‑1 (MQ=255)
                                cGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCa                                                                                                                  <  2:2559186/150‑1 (MQ=255)
                                 gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCag                                                                                                                 <  1:4885154/150‑1 (MQ=255)
                                 gATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCag                                                                                                                 <  1:3977435/150‑1 (MQ=255)
                                    aaCTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                                                              >  2:590454/1‑150 (MQ=255)
                                       tttGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                           <  1:4127510/150‑1 (MQ=255)
                                         tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGcgg                                                                                                         >  1:3282882/1‑148 (MQ=255)
                                           aTACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGcggtt                                                                                                       >  2:906710/1‑146 (MQ=255)
                                             aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacc                                                                                                     <  2:359387/150‑1 (MQ=255)
                                             aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacc                                                                                                     <  2:3094720/150‑1 (MQ=255)
                                              ccGGTTTCCAGCAGTTCCTGAGAGATTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacct                                                                                                    <  2:2294/150‑1 (MQ=255)
                                                 gTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctacc                                                                                                 <  2:4200266/150‑1 (MQ=255)
                                                  tttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCCCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCg                                                                                                <  2:4277934/150‑1 (MQ=255)
                                                       agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACt                                                                                           <  1:4267344/150‑1 (MQ=255)
                                                       agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCGGGGCt                                                                                           >  1:3326523/1‑150 (MQ=255)
                                                          agTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGACttaa                                                                                        >  2:2831485/1‑148 (MQ=255)
                                                            ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAAt                                                                                      <  2:4548074/150‑1 (MQ=255)
                                                             tCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATc                                                                                     <  1:3687554/150‑1 (MQ=255)
                                                             tCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACAACCCGGAcataaaaa                                                                                     >  1:2481057/1‑143 (MQ=255)
                                                              ccTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCg                                                                                    <  2:4024986/150‑1 (MQ=255)
                                                              ccTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTAACGGGCCTGCAAtag                                                                                    >  1:1184981/1‑148 (MQ=255)
                                                                tGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCggg                                                                                  <  1:1584603/150‑1 (MQ=255)
                                                                 gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGgt                                                                                 <  2:3639478/150‑1 (MQ=255)
                                                                 gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACTACCGGGGCTTAAAAAGGgt                                                                                 >  1:1558043/1‑150 (MQ=255)
                                                                 gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCCCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTACCGGGACTTCAATCgggg                                                                                 >  1:2939757/1‑149 (MQ=255)
                                                                  agagTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTACCCGGACATCAATAGGgtg                                                                                >  2:1141560/1‑150 (MQ=255)
                                                                     gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                                                             <  1:189835/150‑1 (MQ=255)
                                                                     gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTc                                                                             <  1:4518339/150‑1 (MQ=255)
                                                                        tGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCAACCGGGACGTCCATAGGGTGTTCGAg                                                                          >  2:1954636/1‑150 (MQ=255)
                                                                             gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAATCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCtt                                                                     <  2:2481290/150‑1 (MQ=255)
                                                                              ctctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACAAACGGGGCTTCAATACGGTGGTCGGGGTCttt                                                                    >  2:4125482/1‑150 (MQ=255)
                                                                               tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCtttt                                                                   <  1:500151/150‑1 (MQ=255)
                                                                               tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCtttt                                                                   <  1:4590990/150‑1 (MQ=255)
                                                                               tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCtttt                                                                   <  1:1818700/150‑1 (MQ=255)
                                                                               tctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACAACCCGGCCATCAAACGGGTGTTCGATGtggttt                                                                   >  1:4382172/1‑145 (MQ=255)
                                                                                ctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTa                                                                  <  2:685561/150‑1 (MQ=255)
                                                                                 ttCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTAc                                                                 <  2:2969502/150‑1 (MQ=255)
                                                                                   cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTTCAt                                                               >  2:4433779/1‑150 (MQ=255)
                                                                                    gTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCGGGACTTCAATAGGGTGTTCGAGGTCTTTTTCATc                                                              >  2:1516204/1‑150 (MQ=255)
                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCa                                                            <  2:4637301/150‑1 (MQ=255)
                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCCGGACGTCAAGCGGGGGTTCGCGGGCTTTTAACTCCa                                                            >  1:3375090/1‑150 (MQ=255)
                                                                                      aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACGACCGGGACTTAAATCGGGTGTTGGAGGTGTTTtacataca                                                            >  1:2641190/1‑150 (MQ=255)
                                                                                       ggAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTAACGGGGCTTCAATCGGGTGTTGGAGGTGGTTTTCATACAg                                                           >  1:3210041/1‑150 (MQ=255)
                                                                                         aaGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGAc                                                         <  1:1380112/150‑1 (MQ=255)
                                                                                         aaGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCGACCCGGGCGTCAATCGGGTGTTGGAGGTGTGTTTCATACAGAc                                                         >  1:3000498/1‑150 (MQ=255)
                                                                                          aGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACAACCCGGACGTCAATCGGGTGTTAGAGGTCTTTTACATCCACAcc                                                        >  1:3819811/1‑150 (MQ=255)
                                                                                           ggTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATAGGGTGTTAGAGGTCTTTTAAATACAGACcg                                                       >  2:1747299/1‑150 (MQ=255)
                                                                                            gTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTAGAGGTCTTTTACATACAGACcgc                                                      >  2:834797/1‑150 (MQ=255)
                                                                                              gctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGa                                                    <  1:3086004/150‑1 (MQ=255)
                                                                                                  cgcgGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCa                                                <  2:4902961/150‑1 (MQ=255)
                                                                                                     ggTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGAc                                             <  1:2704952/150‑1 (MQ=255)
                                                                                                       tGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCg                                           <  1:5198347/150‑1 (MQ=255)
                                                                                                         aaTCGCCCAAAGCTCTTCTTCAACGATCTCGCCTTTCATGTCTACAGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCACTCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTc                                         <  1:4742647/150‑1 (MQ=255)
                                                                                                           tCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTAACCGGACATAAATAGGGTGGTGGTGGTGTTTTAAATACAGACCGCCAAGCAGAACggccg                                       >  1:745941/1‑146 (MQ=255)
                                                                                                              cccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCggagg                                    <  1:1216748/150‑1 (MQ=255)
                                                                                                              cccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCggagg                                    <  2:580345/150‑1 (MQ=255)
                                                                                                                  aagCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTAACGGGGCATAAAACGGGTGTTAGAGGTCTTTTTAAAACAGACCGCGAAGCACACCGTCGGAGGAAcc                                >  2:3598792/3‑150 (MQ=255)
                                                                                                                  aagCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACTACCAGGACATCAAAAGGGTGGTGGGGGTCTTTTAAATACAGACCCCGAACCAGACCCTCCGAGGaaac                                >  1:3584944/3‑148 (MQ=255)
                                                                                                                   agCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTAACGGGACGTACATCGGGTGTTCGAGGTCAGTGACATCCAGACCGCGACGCAGACCGTCGGAGGAAcac                               >  2:3037923/2‑148 (MQ=255)
                                                                                                                    gCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCa                              <  1:1013981/150‑1 (MQ=255)
                                                                                                                      tcttctTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAtt                            <  2:3175570/150‑1 (MQ=255)
                                                                                                                      tcttctTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAtt                            <  1:2310948/150‑1 (MQ=255)
                                                                                                                      tcttctTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAtt                            <  2:414570/150‑1 (MQ=255)
                                                                                                                      tcttctTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACAAACCGGCCATCAATAGGGTGTTAGAGGTCTTTTAAAAACAGACCCCGACGCAGACCCTCCGAGGGACCCaaa                            >  1:497652/1‑148 (MQ=255)
                                                                                                                       cttcttCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTAACGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATACAGACCGCGACGCACACCGTCGGAGGAACCCATTg                           >  2:4363000/1‑150 (MQ=255)
                                                                                                                        ttcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGc                          <  2:5049517/150‑1 (MQ=255)
                                                                                                                        ttcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGc                          <  1:3126296/150‑1 (MQ=255)
                                                                                                                        ttcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGc                          <  1:1770994/150‑1 (MQ=255)
                                                                                                                           ttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCTACCGGGCCTTCAATCGGGTGTTAGAGGTCTTTTTCATCCAGACCGCGACGCAGACCCTCGGAGGAAACCAATGCGAt                       >  2:4308584/1‑150 (MQ=255)
                                                                                                                               ccGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgta                   <  2:2357113/150‑1 (MQ=255)
                                                                                                                               ccGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGCCGCTTTACCTACCGGGGCTTCAATCGGGTGTTCGAGGGCTTTTTCATCCAGACCGCGACGCAGACCGTCGGGGGAAACCATTGCGATGgta                   >  2:1873664/1‑150 (MQ=255)
                                                                                                                                cGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCCGGACTTCAATCGGGTGTTGGAGGTCTTTTACATCCAGACCGCCACGCAGACCGTCGGAGGAACCCAATGCGATGgtac                  >  2:2646058/1‑150 (MQ=255)
                                                                                                                                 gATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacg                 <  2:133384/150‑1 (MQ=255)
                                                                                                                                         cTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACGACCGGGACTTCAAAAGGGTGTTAGAGGTGTTTTAAAACCCGACCCCCACGCACACCGTCGGCGGGACCCAATGCGAAGGGACGTACGATAc         >  1:5304884/1‑150 (MQ=255)
                                                                                                                                          tttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAcc        <  2:1936899/150‑1 (MQ=255)
                                                                                                                                              aTGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccgccg    <  1:3893863/150‑1 (MQ=255)
                                                                                                                                              aTGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTGTACCAAACCGGTCATCAATAGGGTGGTCGAGGTATTTTAAATACAAACCGCGACGCACACCCTCGGGCTAACCCCATGCGATGGTACGTAAGAGAcccccg    >  2:2222379/1‑150 (MQ=255)
                                                                                                                                               tGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCCGGACATAAAACGGGTGTTCGAGGTCTTTTAAAATAAGACCGCGACGCAGAACGGCGGAGGAAACCATTTCGAAGGTACGTACGATAccgccgc   >  2:210742/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCGCC  >  CP009273/3910301‑3910594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑