Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,916,830 C→A 100% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,916,8300CA100.0% 1068.8 / NA 320D71Y (GAT→TAT) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (143/177);  total (143/177)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 320 total aligned reads displayed.

TGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGT  >  CP009273/3916684‑3916976
                                                                                                                                                  |                                                                                                                                                  
tGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                                 <  2:2121042/150‑1 (MQ=255)
 ggTTCAATATTCTCCAGTTTAATCTCATGTTGCACCTGACGAAGGAAACGCACTCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATa                                                                                                                                                >  2:2370006/1‑150 (MQ=255)
  gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                               >  1:401402/1‑150 (MQ=255)
    tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAc                                                                                                                                             >  2:5180960/1‑150 (MQ=255)
     cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                            >  2:4842201/1‑150 (MQ=255)
     cAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                            <  1:814291/150‑1 (MQ=255)
      aaTATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCg                                                                                                                                           >  1:3274903/1‑150 (MQ=255)
         atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCTGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                        <  2:4790717/150‑1 (MQ=255)
         atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                        <  2:3187356/150‑1 (MQ=255)
         atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                        <  1:5283095/150‑1 (MQ=255)
          ttCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCa                                                                                                                                       <  2:3400156/150‑1 (MQ=255)
           tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGGTCAc                                                                                                                                      >  2:4604040/1‑150 (MQ=255)
            ctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAcc                                                                                                                                     >  1:1210813/1‑150 (MQ=255)
              ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAATCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                                   <  1:4138475/150‑1 (MQ=255)
              ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                                   >  2:2941489/1‑150 (MQ=255)
              ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                                   >  1:4272363/1‑150 (MQ=255)
                 gTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCa                                                                                                                                <  2:2044818/150‑1 (MQ=255)
                  tttAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAg                                                                                                                               >  2:4075281/1‑150 (MQ=255)
                    tAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGAt                                                                                                                             <  1:56004/150‑1 (MQ=255)
                      aGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATAc                                                                                                                           >  2:881819/1‑150 (MQ=255)
                        cTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                         >  2:5023320/1‑150 (MQ=255)
                         tCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGt                                                                                                                        >  2:448468/1‑150 (MQ=255)
                          cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTg                                                                                                                       <  1:1161992/150‑1 (MQ=255)
                           aTGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                      >  1:4878906/1‑150 (MQ=255)
                           aTGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGc                                                                                                                      <  1:3869169/150‑1 (MQ=255)
                                    cTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAAt                                                                                                             <  2:3617885/150‑1 (MQ=255)
                                    cTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAAt                                                                                                             <  2:2643901/150‑1 (MQ=255)
                                     tGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                            >  2:4574270/1‑150 (MQ=255)
                                     tGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGAGCCACCACAATg                                                                                                            >  1:4751914/1‑150 (MQ=255)
                                       aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCt                                                                                                          <  2:3851126/150‑1 (MQ=255)
                                       aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCt                                                                                                          <  1:247469/150‑1 (MQ=255)
                                        cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTa                                                                                                         <  1:2706761/150‑1 (MQ=255)
                                         caaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGAAAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                        >  2:4528656/2‑150 (MQ=255)
                                          aaGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATc                                                                                                       >  2:1967673/1‑150 (MQ=255)
                                             gAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgag                                                                                                    >  1:998201/1‑150 (MQ=255)
                                                  gcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                                               >  1:4439686/1‑150 (MQ=255)
                                                     cgcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGc                                                                                            <  2:1347646/150‑1 (MQ=255)
                                                           tACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                      >  1:2107502/1‑150 (MQ=255)
                                                           tACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                      <  1:612222/150‑1 (MQ=255)
                                                           tACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGCCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                      >  1:1217904/1‑150 (MQ=255)
                                                            aCCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAg                                                                                     >  2:2284403/1‑150 (MQ=255)
                                                             ccAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGc                                                                                    <  1:3616878/150‑1 (MQ=255)
                                                             ccAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGc                                                                                    <  2:4294513/150‑1 (MQ=255)
                                                               aaGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                  >  1:4616542/1‑150 (MQ=255)
                                                                   cTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                              <  1:5029462/150‑1 (MQ=255)
                                                                    tATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                             <  2:4228243/150‑1 (MQ=255)
                                                                    tATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                             <  2:3065814/150‑1 (MQ=255)
                                                                     aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAtt                                                                            >  2:2209833/1‑150 (MQ=255)
                                                                     aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAtt                                                                            <  2:1368863/150‑1 (MQ=255)
                                                                     aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAtt                                                                            <  1:3704687/150‑1 (MQ=255)
                                                                        cAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAgg                                                                         >  2:4753014/1‑150 (MQ=255)
                                                                         aaCAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGa                                                                        >  2:1622943/1‑150 (MQ=255)
                                                                          aCAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGAt                                                                       <  2:3452835/150‑1 (MQ=255)
                                                                           cAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATc                                                                      <  1:2639498/150‑1 (MQ=255)
                                                                            agagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                                     >  2:817871/1‑150 (MQ=255)
                                                                            agagTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                                     >  2:3492514/1‑150 (MQ=255)
                                                                              agTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                                   >  1:1599976/1‑150 (MQ=255)
                                                                              agTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                                   >  2:2926272/1‑150 (MQ=255)
                                                                                 gAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGAc                                                                >  2:1177668/1‑150 (MQ=255)
                                                                                   aaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCg                                                              <  2:2458195/150‑1 (MQ=255)
                                                                                    aTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGa                                                             >  1:1961655/1‑150 (MQ=255)
                                                                                          ttCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAg                                                       >  2:612523/1‑150 (MQ=255)
                                                                                            cAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGt                                                     >  2:1039100/1‑150 (MQ=255)
                                                                                             aGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                                    >  1:1102486/1‑150 (MQ=255)
                                                                                              ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTg                                                   <  2:2732177/150‑1 (MQ=255)
                                                                                              ggACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTg                                                   >  1:837625/1‑150 (MQ=255)
                                                                                               gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAAAGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAtgttgt                                                  >  1:1692702/1‑150 (MQ=255)
                                                                                                aCGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                                 <  1:2850525/150‑1 (MQ=255)
                                                                                                aCGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTa                                                 <  1:3768349/150‑1 (MQ=255)
                                                                                                     cGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCtt                                            <  2:3536376/150‑1 (MQ=255)
                                                                                                       aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                          >  2:180959/1‑150 (MQ=255)
                                                                                                       aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTg                                          >  1:1773710/1‑150 (MQ=255)
                                                                                                        tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                                         <  2:1134222/150‑1 (MQ=255)
                                                                                                         agagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                                        <  2:1468604/150‑1 (MQ=255)
                                                                                                          gagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTc                                       <  1:2957381/150‑1 (MQ=255)
                                                                                                          gagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTc                                       <  1:1472531/150‑1 (MQ=255)
                                                                                                               tGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCAttt                                  <  1:3362724/150‑1 (MQ=255)
                                                                                                                 gAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt                                <  2:508275/150‑1 (MQ=255)
                                                                                                                 gAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt                                <  1:138627/150‑1 (MQ=255)
                                                                                                                     gCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcag                            <  2:4893255/150‑1 (MQ=255)
                                                                                                                     gCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcag                            <  1:2353545/150‑1 (MQ=255)
                                                                                                                     gCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcag                            <  2:1210666/150‑1 (MQ=255)
                                                                                                                        tGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCat                         <  1:3418128/150‑1 (MQ=255)
                                                                                                                          gCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCatat                       >  1:1016916/1‑150 (MQ=255)
                                                                                                                            aGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                     <  2:3717443/150‑1 (MQ=255)
                                                                                                                             gTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa                    <  1:3025575/150‑1 (MQ=255)
                                                                                                                              tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAc                   <  1:3136356/150‑1 (MQ=255)
                                                                                                                                  ggTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAg               <  2:5251432/150‑1 (MQ=255)
                                                                                                                                   gTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAgg              <  2:532618/150‑1 (MQ=255)
                                                                                                                                    tCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGc             <  1:2678275/150‑1 (MQ=255)
                                                                                                                                       ggTGCAGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAAt          <  1:4341214/150‑1 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCATATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATa         <  2:3760451/150‑1 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATTCAGCATATTCACGTAGGCAATa         <  2:2598541/150‑1 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCATCATATTCACGTAGGCAATa         <  1:5116052/150‑1 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATa         <  2:4181340/150‑1 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATa         >  1:4213858/1‑150 (MQ=255)
                                                                                                                                        gTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATa         <  1:4497469/150‑1 (MQ=255)
                                                                                                                                         tGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATaa        >  1:2383970/1‑150 (MQ=255)
                                                                                                                                         tGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATaa        <  1:881819/150‑1 (MQ=255)
                                                                                                                                            cGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt     >  1:2244764/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGT  >  CP009273/3916684‑3916976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑