BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,936,834	G→T	20.8%	noncoding (40/76 nt)	gltU →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,936,834	0	G	T	20.8%	198.6 / 64.6	153	noncoding (40/76 nt)	gltU	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base G (68/53); new base T (16/16); total (84/69)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

AACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTA  >  CP009273/3936685‑3936904

aaCCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcg                                                                        >  2:3234534/1‑150 (MQ=17)

   cTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcggcg                                                                     >  2:5012847/1‑150 (MQ=17)

     gCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGt                                                                   <  1:4625220/150‑1 (MQ=17)

        gTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAAc                                                                >  2:2727399/1‑150 (MQ=17)

        gTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                                >  1:1255908/1‑150 (MQ=17)

        gTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                                >  1:5074603/1‑150 (MQ=17)

           ggATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgg                                                             >  2:3855803/1‑150 (MQ=17)

             aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAgggg                                                           <  1:5055960/150‑1 (MQ=17)

             aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgggg                                                           >  1:4834794/1‑150 (MQ=17)

              tCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGt                                                          >  1:1066951/1‑150 (MQ=17)

               cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                         <  2:3741885/150‑1 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGACGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTACCAGGGGTTc                                                        >  2:2422795/1‑150 (MQ=12)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGTGGCCCTGGACACCGCCCTTTAACGGCGGTAACAGGGGTTc                                                        <  2:1174691/150‑1 (MQ=12)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTc                                                        <  1:277271/150‑1 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTc                                                        <  1:108685/150‑1 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTc                                                        <  1:211499/150‑1 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTc                                                        <  2:5303589/150‑1 (MQ=17)

                  ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGa                                                      >  2:1645961/1‑150 (MQ=17)

                  ctcctTACCTTAAAGAAGCGTTCTATGCAGTGCTCACACCGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGa                                                      >  1:909416/1‑150 (MQ=255)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATc                                                   <  2:3490824/150‑1 (MQ=17)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATc                                                   >  1:778452/1‑150 (MQ=17)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATc                                                   <  2:5298884/150‑1 (MQ=17)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATc                                                   >  2:3142626/1‑150 (MQ=17)

                      ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATcc                                                  <  1:2640693/150‑1 (MQ=17)

                       taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATccc                                                 >  1:515014/1‑150 (MQ=17)

                        accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcccc                                                >  2:2133917/1‑150 (MQ=17)

                        accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcccc                                                >  2:5119874/1‑150 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                               <  2:3810194/150‑1 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                               >  2:4648376/1‑150 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                               <  1:3327470/150‑1 (MQ=17)

                           ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGACCTTTAACTGCGGTAACAGGGCTTCGAATCCCCTAg                                             >  2:3290447/1‑150 (MQ=255)

                             aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                           >  2:3698696/1‑150 (MQ=17)

                              aagaagCGTTCTTTGCAGTGCTCAGTAAGATTGTCTGTTAGGTAGTGATTTGCATGGCGTATTGCGATGCAGTATGATACGTCCCCTTGGTCTAGAGGCCCAGGTCTCCGGTCTTTTTCGGCGGTAACAGGGGTTCGTATCCCCTTggtg                                          <  2:4852211/150‑3 (MQ=2)

                              aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                          >  2:2463777/1‑150 (MQ=17)

                              aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                          >  1:3433745/1‑150 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTATAGGCCCAGGACACCGCCCTTTAAAGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                         >  1:2112683/1‑150 (MQ=12)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGACTCCCCTAGGGGa                                         >  1:1876420/1‑150 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                         >  1:2331683/1‑150 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                         >  1:1780394/1‑150 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                        >  2:3015852/1‑150 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                        >  2:1305132/1‑150 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACg                                       >  1:2978430/1‑150 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACg                                       <  2:1997478/150‑1 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACg                                       >  2:889570/1‑150 (MQ=17)

                                  agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                      >  2:5165234/1‑150 (MQ=17)

                                   gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGcc                                     >  1:4227716/1‑150 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                    >  1:3083084/1‑150 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                    >  1:5000883/1‑150 (MQ=17)

                                     gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                   >  1:1648020/1‑150 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                 >  2:1166603/1‑150 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                 <  1:417034/150‑1 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt                                 <  1:1462979/150‑1 (MQ=17)

                                        cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTg                                >  1:2108673/1‑150 (MQ=17)

                                           tGCAGTGCTCACACAGATTGTGTGATAGGATGTGAACCGCAAGGCGTCTTGCGAAGCAGTCTGATCCGTCCCCGTCGTCTTTCGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                             <  1:4044451/150‑1 (MQ=11)

                                           tGCAGTGCTCACACAGATTGTCTGATCGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCACCTAGGGGACGCCACTGGCTg                             >  2:306264/1‑150 (MQ=255)

                                           tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                             <  2:4793557/150‑1 (MQ=17)

                                           tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                             <  1:1261956/150‑1 (MQ=17)

                                           tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                             <  1:4044498/150‑1 (MQ=17)

                                             cAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCCCGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGt                           <  2:1910257/150‑1 (MQ=255)

                                              aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                          >  1:5273416/1‑150 (MQ=17)

                                              aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                          >  2:1376645/1‑150 (MQ=17)

                                               gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                         <  1:3536419/150‑1 (MQ=17)

                                               gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                         <  1:826090/150‑1 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        >  2:2518688/1‑150 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        >  2:1480936/1‑150 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        >  2:1480866/1‑150 (MQ=17)

                                                 gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTCACAGGGGTTCGATTCCCCTGGGGGACGCCACTTGCtggttggt                       >  2:3548623/1‑150 (MQ=11)

                                                 gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgt                       >  1:80341/1‑150 (MQ=17)

                                                    cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTACGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                    >  1:4852600/1‑150 (MQ=17)

                                                     acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                   <  2:4540353/150‑1 (MQ=17)

                                                     acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                   <  2:381365/150‑1 (MQ=17)

                                                     acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                   <  2:5158258/150‑1 (MQ=17)

                                                      cacaGATTGTCTGAGAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtg                  >  2:4920314/1‑150 (MQ=17)

                                                       acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                 <  2:1014181/150‑1 (MQ=17)

                                                        caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                >  2:2168666/1‑150 (MQ=17)

                                                        caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                >  1:2738647/1‑150 (MQ=17)

                                                         agagTGTCTGATAGGACGTGAACAGCAAGGCGTCTTGCGACGCAGACGGTTACGTCCCCTTCGTCGAGAGGCCCAGGACACCGCCCGTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               <  2:4418886/150‑1 (MQ=11)

                                                         aGATTGTCTGATAGGTAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               <  2:4313080/150‑1 (MQ=17)

                                                         aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTGTGTGAGTGaaa               >  2:4009990/1‑150 (MQ=17)

                                                         aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               >  1:554936/1‑150 (MQ=17)

                                                         aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               >  2:3379659/1‑150 (MQ=17)

                                                           aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt             >  1:2031311/1‑150 (MQ=17)

                                                             tGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTct           <  2:371007/150‑2 (MQ=17)

                                                              gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc          >  2:3571933/1‑150 (MQ=17)

                                                              gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc          <  2:2796973/150‑1 (MQ=17)

                                                                cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt        <  2:4060251/150‑1 (MQ=17)

                                                                 tGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTg       >  2:1046265/1‑150 (MQ=32)

                                                                 tGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTg       <  2:5031791/150‑1 (MQ=17)

                                                                    tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt    <  1:2544031/150‑1 (MQ=17)

                                                                    tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt    <  1:50106/150‑1 (MQ=17)

                                                                    tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt    <  2:4332975/150‑1 (MQ=17)

                                                                    tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt    <  2:4109557/150‑1 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   >  1:1155160/1‑150 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   >  1:1154986/1‑150 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   <  1:2933954/150‑1 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   <  1:4482852/150‑1 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   <  1:4365050/150‑1 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt   <  1:3655384/150‑1 (MQ=17)

                                                                      ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTa  <  1:74841/150‑1 (MQ=14)

                                                                      ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTa  >  1:2997251/1‑150 (MQ=14)

AACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTA  >  CP009273/3936685‑3936904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`