Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,966,854 C→A 100% R325S (CGT→AGT)  rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,966,8540CA100.0% 1000.7 / NA 301R325S (CGT→AGT) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (169/132);  total (169/132)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 301 total aligned reads displayed.

AACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGC  >  CP009273/3966707‑3967000
                                                                                                                                                   |                                                                                                                                                  
aaCAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGt                                                                                                                                                  >  1:5226445/1‑150 (MQ=255)
 aCAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTa                                                                                                                                                 <  2:2883650/150‑1 (MQ=255)
  cAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaa                                                                                                                                                >  1:3601910/1‑150 (MQ=255)
  cAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaa                                                                                                                                                >  1:2251340/1‑150 (MQ=255)
   aaGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                               <  1:4865908/150‑1 (MQ=255)
   aaGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaa                                                                                                                                               <  1:3351130/150‑1 (MQ=255)
    aGCCGCACGGCGTGGTGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaaa                                                                                                                                              >  1:4820109/1‑150 (MQ=255)
    aGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaaa                                                                                                                                              >  2:1547465/1‑150 (MQ=255)
     gCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAAc                                                                                                                                             <  2:4599162/150‑1 (MQ=255)
         cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGtt                                                                                                                                         >  2:2167034/1‑150 (MQ=255)
          aCGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTc                                                                                                                                        <  1:4635008/150‑1 (MQ=255)
           cGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCa                                                                                                                                       <  1:3818208/150‑1 (MQ=255)
            ggCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAg                                                                                                                                      <  1:3850397/150‑1 (MQ=255)
            ggCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAg                                                                                                                                      >  1:2956187/1‑150 (MQ=255)
             gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGt                                                                                                                                     >  2:3260871/1‑150 (MQ=255)
                   cgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATc                                                                                                                               >  2:686834/1‑150 (MQ=255)
                    gcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCt                                                                                                                              >  2:1118941/1‑150 (MQ=255)
                     cACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTg                                                                                                                             <  2:3317123/150‑1 (MQ=255)
                       cTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGc                                                                                                                           >  2:3074981/1‑150 (MQ=255)
                           cGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTAATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAAt                                                                                                                       <  1:3000163/150‑1 (MQ=255)
                             tGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                                                                                                     >  2:838585/1‑150 (MQ=255)
                              gACCTGATCACCTGTGTCGCGGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTACAACGGTTCAGTGGTATCTGGCTAATGaa                                                                                                                    <  2:1219681/150‑1 (MQ=255)
                               aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa                                                                                                                   >  1:3564014/1‑150 (MQ=255)
                                ccTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg                                                                                                                  <  2:4788370/150‑1 (MQ=255)
                                 cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                                                                                                                 >  2:1800229/1‑150 (MQ=255)
                                    aTCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                                                                                                              >  1:316460/1‑150 (MQ=255)
                                     tCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggt                                                                                                             >  1:1832274/1‑150 (MQ=255)
                                      cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtg                                                                                                            >  2:2049797/1‑150 (MQ=255)
                                          tttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAATCGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAg                                                                                                        >  1:360436/1‑150 (MQ=255)
                                              tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg                                                                                                    >  2:3858837/1‑150 (MQ=255)
                                                gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTg                                                                                                  <  2:4415550/150‑1 (MQ=255)
                                                  tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa                                                                                                >  1:1669753/1‑150 (MQ=255)
                                                   gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg                                                                                               <  2:3548761/150‑1 (MQ=255)
                                                       gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                           <  2:1821514/150‑1 (MQ=255)
                                                       gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACg                                                                                           >  2:3238396/1‑150 (MQ=255)
                                                          cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGACTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCa                                                                                        >  1:1702659/1‑150 (MQ=255)
                                                          cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCa                                                                                        >  1:1232112/1‑150 (MQ=255)
                                                           ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAg                                                                                       >  2:3903037/1‑150 (MQ=255)
                                                             ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCt                                                                                     >  1:1167188/1‑150 (MQ=255)
                                                               cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                   >  2:2463762/1‑150 (MQ=255)
                                                               cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                   >  2:1896206/1‑150 (MQ=255)
                                                               cATGATCTGCGATATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTAGAAAGTGGACTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                   >  2:1774005/1‑150 (MQ=255)
                                                                     cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGc                                                                             <  1:3267691/150‑1 (MQ=255)
                                                                      tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTCTCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCg                                                                            >  1:964828/1‑150 (MQ=255)
                                                                        cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAg                                                                          >  1:2688362/1‑150 (MQ=255)
                                                                              gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTa                                                                    >  2:258185/1‑150 (MQ=255)
                                                                                cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAgg                                                                  >  2:1442638/1‑150 (MQ=255)
                                                                                cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAgg                                                                  <  1:2519973/150‑1 (MQ=255)
                                                                                 aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                                 >  2:4453656/1‑150 (MQ=255)
                                                                                 aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                                 >  2:4644317/1‑150 (MQ=255)
                                                                                     aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGa                                                             >  2:3411403/1‑150 (MQ=255)
                                                                                     aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGa                                                             <  2:5316215/150‑1 (MQ=255)
                                                                                     aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGa                                                             >  2:785058/1‑150 (MQ=255)
                                                                                        cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAATAGGTTCAGTGGTATCTGGCTAATGAAAGCTGTTGGAAGCTGGTGCAGGACGGCATCTATCAGGGCGAGCGTTTAGGTCTGAAAg                                                          >  2:2324683/1‑150 (MQ=255)
                                                                                        cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAg                                                          <  1:2474113/150‑1 (MQ=255)
                                                                                         ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAgg                                                         <  1:3968244/150‑1 (MQ=255)
                                                                                         ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAgg                                                         <  1:3529372/150‑1 (MQ=255)
                                                                                           cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCt                                                       >  1:1325024/1‑150 (MQ=255)
                                                                                             aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTaa                                                     >  1:1761986/1‑150 (MQ=255)
                                                                                             aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTaa                                                     >  1:1377305/1‑150 (MQ=255)
                                                                                               aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAtt                                                   >  1:4768778/1‑150 (MQ=255)
                                                                                                aTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAttt                                                  >  2:2904843/1‑150 (MQ=255)
                                                                                                  tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTc                                                <  1:3114090/150‑1 (MQ=255)
                                                                                                    cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTCATTTTCAg                                              >  2:2806477/1‑150 (MQ=255)
                                                                                                    cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAg                                              <  2:4549928/150‑1 (MQ=255)
                                                                                                    cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAg                                              <  2:5240377/150‑1 (MQ=255)
                                                                                                     ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGc                                             <  2:2002664/150‑1 (MQ=255)
                                                                                                      cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                            <  1:887108/150‑1 (MQ=255)
                                                                                                      cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                            <  1:397851/150‑1 (MQ=255)
                                                                                                      cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                            <  2:4298404/150‑1 (MQ=255)
                                                                                                        tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                          >  2:2257311/1‑150 (MQ=255)
                                                                                                            cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCa                                      <  2:2646024/150‑1 (MQ=255)
                                                                                                             ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCaa                                     >  1:1461328/1‑150 (MQ=255)
                                                                                                              tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCaaa                                    >  2:564054/1‑150 (MQ=255)
                                                                                                              tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCaaa                                    >  1:5286574/1‑150 (MQ=255)
                                                                                                               ggctggctGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAAc                                   <  1:2079833/150‑1 (MQ=255)
                                                                                                                gctggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACa                                  <  1:3392565/150‑1 (MQ=255)
                                                                                                                  tggctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                                >  1:681772/1‑150 (MQ=255)
                                                                                                                     ctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGaaa                             <  1:2434899/150‑1 (MQ=255)
                                                                                                                       gCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAgg                           <  2:2368110/150‑1 (MQ=255)
                                                                                                                        cctcatgAAACCTTTGAAAGTGGAATGAGTATAACGGTTCAGTGGTATCTGGATAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGt                          <  2:1372494/144‑1 (MQ=255)
                                                                                                                        ccGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGt                          <  2:5134428/150‑1 (MQ=255)
                                                                                                                          gCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtat                        <  1:2899060/150‑1 (MQ=255)
                                                                                                                           cAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatt                       >  2:3666136/1‑150 (MQ=255)
                                                                                                                           cAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtatt                       <  1:1584249/150‑1 (MQ=255)
                                                                                                                             ggAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtattat                     >  2:4491495/1‑150 (MQ=255)
                                                                                                                              naaaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATc                    >  1:4633446/2‑150 (MQ=255)
                                                                                                                              gAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATc                    <  1:2821986/150‑1 (MQ=255)
                                                                                                                               aaaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATcc                   >  2:2852825/1‑150 (MQ=255)
                                                                                                                                aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                  >  1:4069773/1‑150 (MQ=255)
                                                                                                                                aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                  <  1:1299689/150‑1 (MQ=255)
                                                                                                                                aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                  >  2:909263/1‑150 (MQ=255)
                                                                                                                                  ccTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg                <  2:4894187/150‑1 (MQ=255)
                                                                                                                                   cTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggc               >  2:1624953/1‑150 (MQ=255)
                                                                                                                                      tGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCATCGGAGGCAAACATGAAAGGTATTATCCTggcggg            >  2:3757069/1‑150 (MQ=255)
                                                                                                                                      tGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACAAGAAAGGTATTATCCTggcggg            >  1:420525/1‑150 (MQ=255)
                                                                                                                                         aaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGgcgggcgg         >  1:4561391/1‑150 (MQ=255)
                                                                                                                                           gTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGtt       >  2:3575339/1‑150 (MQ=255)
                                                                                                                                            tGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc      <  1:4927721/150‑1 (MQ=255)
                                                                                                                                                aTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGc  >  1:1006615/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACAAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCGGC  >  CP009273/3966707‑3967000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑