Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,718 A→C 8.4% N301T (AAC→ACC)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7180AC 8.4% 617.8 / 9.9 245N301T (AAC→ACC) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (147/71);  new base C (2/25);  total (149/96)
Fisher's exact test for biased strand distribution p-value = 1.29e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGT  >  CP009273/31570‑31867
                                                                                                                                                     |                                                                                                                                                     
ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAAc                                                                                                                                                      <  1:2532177/150‑1 (MQ=255)
 cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAAcc                                                                                                                                                     >  1:4898962/1‑150 (MQ=255)
    cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCAc                                                                                                                                                  <  1:4541157/150‑1 (MQ=255)
       aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                               >  2:4591859/1‑150 (MQ=255)
       aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                               >  2:3851930/1‑150 (MQ=255)
         aCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCgtg                                                                                                                                             <  1:3506026/150‑1 (MQ=255)
          cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCgtgt                                                                                                                                            <  2:4982041/150‑1 (MQ=255)
           gCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTc                                                                                                                                           <  1:4301355/150‑1 (MQ=255)
            cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTcc                                                                                                                                          <  1:3415542/150‑1 (MQ=255)
              gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCg                                                                                                                                        <  1:2810135/150‑1 (MQ=255)
               aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGt                                                                                                                                       >  2:578114/1‑150 (MQ=255)
               aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGt                                                                                                                                       >  2:4060820/1‑150 (MQ=255)
                ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGtt                                                                                                                                      >  2:2590564/1‑150 (MQ=255)
                  gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                                    >  1:1006946/1‑150 (MQ=255)
                  gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                                    <  1:2965839/150‑1 (MQ=255)
                    cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                  >  1:2511676/1‑150 (MQ=255)
                    cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                  >  1:5197145/1‑150 (MQ=255)
                      aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                                >  2:1058782/1‑150 (MQ=255)
                          atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                            >  2:4813766/1‑150 (MQ=255)
                           tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                                           <  1:142292/150‑1 (MQ=255)
                           tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                                           <  2:1784174/150‑1 (MQ=255)
                               aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGTACCCACGCGTGTCCCGTTCTTAGGCGCTGGCGt                                                                                                                       >  2:1178769/1‑150 (MQ=255)
                               aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGt                                                                                                                       <  1:5134386/150‑1 (MQ=255)
                                 aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                                     >  1:1945555/1‑150 (MQ=255)
                                  tCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                                                                    <  2:3681763/150‑1 (MQ=255)
                                    aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                                  >  1:2982148/1‑150 (MQ=255)
                                    aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                                  >  2:3953554/1‑150 (MQ=255)
                                    aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                                  >  1:2219721/1‑150 (MQ=255)
                                     tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGGTCTTCGGCGCTGGCGTCGAAAg                                                                                                                 <  2:2790697/150‑1 (MQ=255)
                                       cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                               >  2:639409/1‑150 (MQ=255)
                                          aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                            <  2:3517708/150‑1 (MQ=255)
                                          aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                            >  2:871236/1‑150 (MQ=255)
                                           aCGCCTCGTTGGGGGTGCTGCGTGAAATCGGCGTTGAAACGGGTGGTTCAAACTTTCAGTTTGCGGTGAACCCTAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCg                                                                                                           <  1:2657203/150‑1 (MQ=255)
                                           aCGCCTCGTGGGCGTTGCTGCGTGAAAGCGGCGTTGAAACCGTTGTTTCAAACGTTCTGTTTGCGGTGACCCAGAAAAACGGTCG‑GCTTATTGTTATTGAATTGAACCAACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCg                                                                                                           <  2:4948530/150‑1 (MQ=255)
                                            cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                                          >  2:3212889/1‑150 (MQ=255)
                                            cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                                          >  2:3812217/1‑150 (MQ=255)
                                                tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTc                                                                                                      >  1:1734553/1‑150 (MQ=255)
                                                  ggtGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                                    >  1:1190914/3‑150 (MQ=255)
                                                    tGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGa                                                                                                  >  1:114242/1‑150 (MQ=255)
                                                       cGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTg                                                                                               >  2:1493899/1‑150 (MQ=255)
                                                         gTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCt                                                                                             >  1:3696142/1‑150 (MQ=255)
                                                         gTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCt                                                                                             >  1:2304008/1‑150 (MQ=255)
                                                            ctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaaa                                                                                          >  1:3195742/1‑150 (MQ=255)
                                                             tgcGTTAAACGGGCGTGTAACCGGGGGGTCCCACCGTACAGTTTGCGGTGACCCCGAAAACCGGTCG‑TCTGATTGTTATACAAATGACCCCAGGCGGGCCCCGTTCTTGGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAg                                                                                         <  2:4580547/150‑1 (MQ=255)
                                                              gcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGt                                                                                        >  2:2892819/1‑150 (MQ=255)
                                                                  taaaTCGGGGTTGAAACCGTTGGTACAAAGGTTCTGTTTGCGGTGAACCCGAAAAAGGGTCG‑TCTTTTTGTTTTATAAATGAACCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTggcg                                                                                    <  2:1143162/149‑1 (MQ=255)
                                                                        ggCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                              >  2:547071/1‑150 (MQ=255)
                                                                            ttGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTgg                                                                          >  2:2517151/1‑150 (MQ=255)
                                                                             tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGc                                                                         >  1:1332919/1‑150 (MQ=255)
                                                                                cccgggtggtTCCACTGTTCTGTTTGCGGTTACCCCCAAAAAGGGTCG‑TTTTTTTTTTATATAAATGACCCCCCGGGGGGCCGGTTCTGCGGCGCGGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                                      <  1:5120226/146‑1 (MQ=255)
                                                                                     gtggtTCCACCGTTCTGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGtt                                                                 <  2:3247753/150‑1 (MQ=255)
                                                                                     gtggtTCCAACGTTCTGTTTGCGTTGACCCCGAAAACCGGTCG‑TCTGATTGTTATAGAAATGACCCCACGCGTGCCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGtt                                                                 <  1:830530/150‑1 (MQ=255)
                                                                                            cAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCt                                                          >  1:3777638/1‑150 (MQ=255)
                                                                                            cAACGTTCAGTTGGCGGTGACCCCTAAAACCGGTCG‑TCTGTTTGTTATATAAATGACCCCCCGGGGGCCCCGTTCTGCGGCGCTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCt                                                          <  1:2873629/150‑1 (MQ=255)
                                                                                             accGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                                         <  2:3410084/148‑1 (MQ=255)
                                                                                             aaCGTTCAGTTTGCGGTGACCCCTAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                                         <  2:4389701/150‑1 (MQ=255)
                                                                                             aaCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                                         >  1:2611424/1‑150 (MQ=255)
                                                                                              aCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                                                        >  2:3125301/1‑150 (MQ=255)
                                                                                                gTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                                      >  1:2256911/1‑150 (MQ=255)
                                                                                                 ttCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacg                                                     >  2:2705067/1‑150 (MQ=255)
                                                                                                  tCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacga                                                    >  2:5284751/1‑150 (MQ=255)
                                                                                                   ctgTTTGCGGTTAACCCTAAAACCGGTCG‑TCTGTTTTTTATATAAATGACCCCCCGGGGGCCCCGTTCTTCGGCGGGGGGGACTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                                   <  2:2304008/148‑1 (MQ=255)
                                                                                                    tgTTTGCTGTGACCCCTAAAATTGTTCT‑TTTTTTTTTTATTTAAATTAACCCCGGGGGGTCCGTTTCTTGGGGGGTGGGGTCTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                                  <  2:1829562/149‑1 (MQ=255)
                                                                                                     gTTTGGGGTCACCCCAAAAAGGGGTTG‑TTTTTTTTTTATTTAAATGAACCCCCGCGGGTCCGTTTCTGCGGCGTGGGGTTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACt                                                 <  1:622213/150‑1 (MQ=255)
                                                                                                      tttGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                                >  1:1768512/1‑150 (MQ=255)
                                                                                                        ggCGGTCAACCCTAAAACGGGTCG‑TCTGTTTTTTATAGACAGCCCCCCCCGCGGGCCCGGTTCTGCGGCGCGGGCGCCCACCGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgat                                              <  1:4565820/149‑1 (MQ=255)
                                                                                                          cGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgatga                                            >  2:194361/1‑150 (MQ=255)
                                                                                                          cGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgatga                                            >  2:4682410/1‑150 (MQ=255)
                                                                                                           ggTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                                           >  1:541872/1‑150 (MQ=255)
                                                                                                           ggTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGaa                                           >  2:4291611/1‑150 (MQ=255)
                                                                                                            gttacccccAAAAACGGTGG‑TCTGATTTTTATAGAAATCAACCCCGGCGGGTCCCGTTCTGGGGCGCGGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                                          <  1:1592437/145‑1 (MQ=255)
                                                                                                            gTGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                                          <  2:2628913/150‑1 (MQ=255)
                                                                                                             ttaaCCCTAAAACGGGTGG‑TCTGTTTGTTTTATAAATTACCCCCCGCGGGCCCCGTTCTTGGGGGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                                         <  1:1266855/148‑1 (MQ=255)
                                                                                                             tGAACCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                                         >  1:2023360/1‑150 (MQ=255)
                                                                                                              gAACCCGAAAAACGGTCG‑TCTGATTGTTATATAAAAGAACCCACGCGTGTCCCGTTCTTCGGCGCAGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                                        >  1:4523442/1‑150 (MQ=255)
                                                                                                                aCCCGAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACa                                      >  2:2898860/1‑150 (MQ=255)
                                                                                                                    gAAAAACGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCAc                                  >  2:165559/1‑150 (MQ=255)
                                                                                                                      aaaaCGGTTGTTCTTTTTTTTATATAATTGACCCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                                 <  2:5110667/150‑1 (MQ=255)
                                                                                                                      aaaaCGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTg                                >  2:1547129/1‑150 (MQ=255)
                                                                                                                      aaaaCGGTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACCACATCACtc                                >  1:3254029/1‑149 (MQ=255)
                                                                                                                       aaccGGTCG‑TCTGATTTTTATATAAATTAACCCCCGCGGGCCCCGTTATTGGGCTCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCTGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTgg                               <  1:4758475/147‑1 (MQ=255)
                                                                                                                           ggTCG‑TCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGa                           >  2:3095248/1‑150 (MQ=255)
                                                                                                                            gtcg‑tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc                          >  2:2065046/1‑150 (MQ=255)
                                                                                                                              cg‑tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt                        >  1:1014825/1‑150 (MQ=255)
                                                                                                                                 tctttttGTTATAAAAATGACCCCCCGCGTGGCCCGTTCTTCGGCGCGGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTAc                      <  1:4727942/145‑1 (MQ=255)
                                                                                                                                     aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcag                  >  1:3617501/1‑148 (MQ=255)
                                                                                                                                       tGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc                >  2:4222616/1‑150 (MQ=255)
                                                                                                                                       tGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc                >  2:4130042/1‑150 (MQ=255)
                                                                                                                                        gTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc               >  1:4973288/1‑150 (MQ=255)
                                                                                                                                          tataGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcctc             >  2:4362974/1‑150 (MQ=255)
                                                                                                                                           atagAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcctcc            >  1:776190/4‑150 (MQ=255)
                                                                                                                                           aaagAAATCACCCCACGGGGGGCCCGTTTGTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcctcc            <  2:2253638/147‑1 (MQ=255)
                                                                                                                                              gAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAACCTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTc         >  2:845385/1‑150 (MQ=255)
                                                                                                                                              gAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTc         >  1:4014344/1‑150 (MQ=255)
                                                                                                                                                 aTGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAg      >  1:3346088/1‑150 (MQ=255)
                                                                                                                                                   gAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTCCTCCGGCCTCCTTCGAGcc    >  2:3558686/1‑150 (MQ=255)
                                                                                                                                                   gAACCCACGCGGGGCCCGTTCTTCGGCGCGGGCGACGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGcc    <  2:12598/150‑1 (MQ=255)
                                                                                                                                                    aaCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCg   >  1:5167694/1‑150 (MQ=255)
                                                                                                                                                    aaCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCg   >  1:4256589/1‑150 (MQ=255)
                                                                                                                                                     aCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGt  >  1:2173831/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCG‑TCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGT  >  CP009273/31570‑31867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑