Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,973,393 C→A 8.0% A26A (GCC→GCA)  wecG → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,973,3930CA 8.0% 1014.0 / 36.2 288A26A (GCC→GCA) wecGUDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
Reads supporting (aligned to +/- strand):  ref base C (119/145);  new base A (12/12);  total (131/157)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

GCTTTTTGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGT  >  CP009273/3973244‑3973542
                                                                                                                                                     |                                                                                                                                                     
 cTTTTTGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGccc                                                                                                                                                      >  1:849949/1‑150 (MQ=255)
  tttttGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCt                                                                                                                                                     <  2:2711177/150‑1 (MQ=255)
   ttttGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTc                                                                                                                                                    <  2:117461/150‑1 (MQ=255)
      tGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCAATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGAt                                                                                                                                                 >  1:4038503/1‑150 (MQ=255)
            cgcCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTg                                                                                                                                           >  1:102271/1‑150 (MQ=255)
             gcCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCTTGATATGCAGCAAGCCCTAGATTATCTGt                                                                                                                                          >  1:1381391/1‑150 (MQ=255)
               cGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGttt                                                                                                                                        <  1:3699175/150‑1 (MQ=255)
                 gACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGc                                                                                                                                      <  1:4850824/150‑1 (MQ=255)
                  aCTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCt                                                                                                                                     <  1:5205299/150‑1 (MQ=255)
                  aCTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCACTCGATTATCTGTTTGCt                                                                                                                                     <  1:2298922/150‑1 (MQ=255)
                   cTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTg                                                                                                                                    <  1:1832393/150‑1 (MQ=255)
                   cTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTg                                                                                                                                    <  2:818587/150‑1 (MQ=255)
                    tcattcatAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGa                                                                                                                                   >  2:1351863/1‑150 (MQ=255)
                     cattcatAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGAc                                                                                                                                  >  2:4288365/1‑150 (MQ=255)
                     cattcatAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGAc                                                                                                                                  <  2:727668/150‑1 (MQ=255)
                      attcatAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCACTCGATTATCTGTTTGCTGACg                                                                                                                                 >  2:2454384/1‑150 (MQ=255)
                      attcatAAACGTACAAAATCATCGCTCCGGACGCAGGATGAAGGATAACAATGAATAACAACACCACGGCACCAACCAATACGCTGCGTGGCTTACAGATGATTGGTTGGCGTGATATGCAGCACGCACACGATTCTCTGTTTGCAGACg                                                                                                                                 >  2:4935495/1‑150 (MQ=255)
                            aaaCGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTTGCGTGATATGCAGCACTCCCTCGATTATCTGTTTGCTGACGGGCAGc                                                                                                                           >  1:1288280/1‑150 (MQ=255)
                            aaaCGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGc                                                                                                                           >  1:4744461/1‑150 (MQ=255)
                             aaCGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCt                                                                                                                          <  1:3653740/150‑1 (MQ=255)
                              aCGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTACCTGTTTGCTGACGGGCAGCtt                                                                                                                         <  1:257446/150‑1 (MQ=255)
                               cGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATATGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTa                                                                                                                        <  1:1784287/150‑1 (MQ=255)
                               cGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTa                                                                                                                        >  1:2991208/1‑150 (MQ=255)
                                gTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTaa                                                                                                                       >  2:3999787/1‑150 (MQ=255)
                                    aaaaTCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCACTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAg                                                                                                                   >  1:3931161/1‑150 (MQ=255)
                                       atcatcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGa                                                                                                                <  2:2161039/150‑1 (MQ=255)
                                       atcatcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGa                                                                                                                >  2:236615/1‑150 (MQ=255)
                                       atcatcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGa                                                                                                                <  1:1728210/150‑1 (MQ=255)
                                         catcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAAc                                                                                                              <  1:512975/150‑1 (MQ=255)
                                         catcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAAc                                                                                                              <  2:4734565/150‑1 (MQ=255)
                                          atcGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACg                                                                                                             >  1:4783406/1‑150 (MQ=255)
                                               tCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGt                                                                                                        <  1:4555618/150‑1 (MQ=255)
                                                 cGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTg                                                                                                      <  2:1962479/150‑1 (MQ=255)
                                                     cGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCAt                                                                                                  <  1:3291509/150‑1 (MQ=255)
                                                      gCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCAtt                                                                                                 <  1:1041536/150‑1 (MQ=255)
                                                       cAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCTTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTa                                                                                                >  2:4952719/1‑150 (MQ=255)
                                                       cAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTa                                                                                                <  1:4943852/150‑1 (MQ=255)
                                                        aGGTTGAAGGATAACAATGAATAACAACACCCCGGCACCAACCTATCCGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCACTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTaa                                                                                               <  2:4556303/150‑1 (MQ=255)
                                                        aGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCAATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATCTGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCACGGAACTCTGGTTGCCATTaa                                                                                               >  2:2783260/1‑150 (MQ=255)
                                                         ggTTGAAGTATAACAATGAATAACAACCCCTCGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGTCGGGCAGCTTAAGCTGGGATCGCTGGTTGCCATTAAt                                                                                              <  2:3915386/150‑1 (MQ=255)
                                                         ggTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAAt                                                                                              <  2:5005574/150‑1 (MQ=255)
                                                          gTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATg                                                                                             <  2:2063136/150‑1 (MQ=255)
                                                          gTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATg                                                                                             >  1:4997287/1‑150 (MQ=255)
                                                            tGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCt                                                                                           <  1:4321927/150‑1 (MQ=255)
                                                              aaGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGa                                                                                         >  2:5224557/1‑150 (MQ=255)
                                                                   tAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGaaaaaa                                                                                    >  2:3157043/1‑150 (MQ=255)
                                                                    aaCAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAAt                                                                                   <  1:2122757/150‑1 (MQ=255)
                                                                            aTAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGTACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTa                                                                           >  1:3800667/1‑150 (MQ=255)
                                                                            aTAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTa                                                                           >  1:1479473/1‑150 (MQ=255)
                                                                             tAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTAt                                                                          >  2:4232434/1‑150 (MQ=255)
                                                                               acaacaCCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGGGATAAGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCCGCTTAAGCATGGAACGCTGGTTGCCATTAATGCTGTAAAAATGCTGACTATTg                                                                        >  1:2315091/1‑150 (MQ=255)
                                                                                caacaCCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGa                                                                       <  2:4877088/150‑1 (MQ=255)
                                                                                caacaCCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGCTGCCATTAATGCTGAAAAAATGCTGACTATTGa                                                                       <  2:4837298/150‑1 (MQ=255)
                                                                                 aacaCCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGaa                                                                      >  2:3306840/1‑150 (MQ=255)
                                                                                   caccacGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTTACTATTGAAGa                                                                    >  2:239699/1‑150 (MQ=255)
                                                                                    accacGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGAt                                                                   <  1:972071/150‑1 (MQ=255)
                                                                                    accacGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGAt                                                                   >  2:2656641/1‑150 (MQ=255)
                                                                                       acGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAAc                                                                <  1:717286/150‑1 (MQ=255)
                                                                                        cGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACg                                                               <  1:3965862/150‑1 (MQ=255)
                                                                                          gCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGcc                                                             <  1:2173880/150‑1 (MQ=255)
                                                                                            aCCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATGGAAGATAACGCCGa                                                           >  2:3218362/1‑150 (MQ=255)
                                                                                              cAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAgg                                                         >  2:4769705/1‑150 (MQ=255)
                                                                                                aCCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTc                                                       <  2:3974435/150‑1 (MQ=255)
                                                                                                aCCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTc                                                       >  1:3163643/1‑150 (MQ=255)
                                                                                                  cTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAg                                                     >  1:4212822/1‑150 (MQ=255)
                                                                                                  cTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAg                                                     >  2:5147320/1‑150 (MQ=255)
                                                                                                      aCGCTGCGTGGCTTACAGTTGTTTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAg                                                 <  2:2755259/150‑1 (MQ=255)
                                                                                                      aCGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAg                                                 <  1:4371615/150‑1 (MQ=255)
                                                                                                       cGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCTCGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGt                                                <  1:2680057/150‑1 (MQ=255)
                                                                                                         cTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGtta                                              <  2:2322719/150‑1 (MQ=255)
                                                                                                           gCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGttaat                                            <  1:2563703/150‑1 (MQ=255)
                                                                                                            cGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGttaatt                                           <  2:2923000/150‑1 (MQ=255)
                                                                                                            cGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCGGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGttaatt                                           <  1:2486740/150‑1 (MQ=255)
                                                                                                             gTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGttaatta                                          >  2:3131253/1‑150 (MQ=255)
                                                                                                             gTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGttaatta                                          <  2:4390686/150‑1 (MQ=255)
                                                                                                                gCTTACAGTTTATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACg                                       <  1:3722079/150‑1 (MQ=255)
                                                                                                                 cTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGc                                      <  1:4364958/150‑1 (MQ=255)
                                                                                                                     ctgTTGATGGGTTGGCGTGATAGGCAGCACGCCCTCGATTATCTGTTTGCGGACGGGCTGCTTAAGCCGGGAACGCGGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGcc                                  <  1:3749964/148‑1 (MQ=255)
                                                                                                                      aGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCg                                 >  1:877203/1‑150 (MQ=255)
                                                                                                                       ttttaTTGGTTGGCGTTATATGAATCACCCCCTCGAATTTATGTTTGCTGACGGGAAGTTTAAGCAGGTAACGATGTTTGCAATTAATGCTTAAAAAATGCTTACTATTTAACATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGa                                <  1:505144/146‑1 (MQ=255)
                                                                                                                          gATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAAtt                             <  1:554109/150‑1 (MQ=255)
                                                                                                                              ggttggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTaaa                         >  2:2233518/1‑150 (MQ=255)
                                                                                                                                ttggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAata                       >  2:2586761/1‑150 (MQ=255)
                                                                                                                                 tggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAatat                      <  1:3221563/150‑1 (MQ=255)
                                                                                                                                 tggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAatat                      <  2:4711919/150‑1 (MQ=255)
                                                                                                                                 tggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGATTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAatat                      <  1:3195117/150‑1 (MQ=255)
                                                                                                                                  ggCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATg                     >  1:3380357/1‑150 (MQ=255)
                                                                                                                                   gCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGc                    <  2:281959/150‑1 (MQ=255)
                                                                                                                                   gCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGc                    <  1:5287631/150‑1 (MQ=255)
                                                                                                                                    cGTGATATGCAGCACGCACTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCg                   >  2:105300/1‑150 (MQ=255)
                                                                                                                                     gTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCgg                  >  1:4705491/1‑150 (MQ=255)
                                                                                                                                       gATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGAt                >  1:4647578/1‑150 (MQ=255)
                                                                                                                                           tGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCa            <  1:811609/150‑1 (MQ=255)
                                                                                                                                            gcagcaCGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCAt           >  1:188938/1‑150 (MQ=255)
                                                                                                                                                caCGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGc       >  1:3600546/1‑150 (MQ=255)
                                                                                                                                                    cccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTg   >  2:59665/1‑150 (MQ=255)
                                                                                                                                                    cccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTg   >  1:1546021/1‑150 (MQ=255)
                                                                                                                                                    cccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGATGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTg   <  1:236615/150‑1 (MQ=255)
                                                                                                                                                     ccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGt  >  2:3267348/1‑150 (MQ=255)
                                                                                                                                                     ccTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTGAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGt  >  2:1869675/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTTTTGAAAGCGCCGGACTCATTCATAAACGTACAAAATCATCGCTCCGGACGCAGGTTGAAGGATAACAATGAATAACAACACCACGGCACCAACCTATACGCTGCGTGGCTTACAGTTGATTGGTTGGCGTGATATGCAGCACGCCCTCGATTATCTGTTTGCTGACGGGCAGCTTAAGCAGGGAACGCTGGTTGCCATTAATGCTGAAAAAATGCTGACTATTGAAGATAACGCCGAGGTCAGGGAGTTAATTAACGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGT  >  CP009273/3973244‑3973542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑