Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,990,354 C→A 6.3% H236Q (CAC→CAA)  xerC → site‑specific tyrosine recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,990,3540CA 6.3% 1104.3 / 26.4 298H236Q (CAC→CAA) xerCsite‑specific tyrosine recombinase
Reads supporting (aligned to +/- strand):  ref base C (144/134);  new base A (8/12);  total (152/146)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

GCGTGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCTCGGT  >  CP009273/3990205‑3990503
                                                                                                                                                     |                                                                                                                                                     
 cGTGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACg                                                                                                                                                      <  1:4302752/150‑1 (MQ=255)
  gTGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGt                                                                                                                                                     >  2:5137056/1‑150 (MQ=255)
   tGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCTCTTTTTATGTCGAAACTGGGAAAGCGTATCTCAGCTCGTAATGTGCAGAAACGCTTTGCCGAATTGGGCATAAAACAATGTCTGAATAATCAAGtt                                                                                                                                                    >  2:4391445/1‑150 (MQ=255)
   tGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGtt                                                                                                                                                    <  1:2103799/150‑1 (MQ=255)
   tGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCAAGtt                                                                                                                                                    >  2:1156299/1‑150 (MQ=255)
   tGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCAAGtt                                                                                                                                                    >  2:3826735/1‑150 (MQ=255)
   tGGATTGAGCACTGGCTTGATGTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGtt                                                                                                                                                    <  2:2368946/150‑1 (MQ=255)
         gAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCg                                                                                                                                              <  2:3534856/150‑1 (MQ=255)
           gCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCa                                                                                                                                            >  1:1017520/1‑150 (MQ=255)
             aCTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATa                                                                                                                                          >  1:3338281/1‑150 (MQ=255)
                ggCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAAt                                                                                                                                       >  1:5143290/1‑150 (MQ=255)
                ggCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAAt                                                                                                                                       >  1:4021012/1‑150 (MQ=255)
                  cTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTa                                                                                                                                     >  1:5203580/1‑150 (MQ=255)
                   ttGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTAc                                                                                                                                    <  2:1216383/150‑1 (MQ=255)
                   ttGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTAc                                                                                                                                    <  1:2479798/150‑1 (MQ=255)
                           cgcgACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCg                                                                                                                            <  1:3779409/150‑1 (MQ=255)
                             cgACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGtt                                                                                                                          <  2:4462742/150‑1 (MQ=255)
                             cgACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGtt                                                                                                                          <  2:2162214/150‑1 (MQ=255)
                              gACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTc                                                                                                                         >  2:2800243/1‑150 (MQ=255)
                              gACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTc                                                                                                                         >  1:3818643/1‑150 (MQ=255)
                               aCCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAAACACGTTCATCCGCATAAATTACGTCACTCGTTCg                                                                                                                        >  2:936058/1‑150 (MQ=255)
                                  tGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCa                                                                                                                     <  1:3139823/150‑1 (MQ=255)
                                  tGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCa                                                                                                                     <  1:2162969/150‑1 (MQ=255)
                                    tttGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACg                                                                                                                   >  1:5012528/1‑150 (MQ=255)
                                     ttGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGc                                                                                                                  >  2:4256935/1‑150 (MQ=255)
                                     ttGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCAAGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGc                                                                                                                  >  2:3372691/1‑150 (MQ=255)
                                          aGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATg                                                                                                             <  2:4222740/150‑1 (MQ=255)
                                          aGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATg                                                                                                             >  1:874667/1‑150 (MQ=255)
                                            cGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCt                                                                                                           >  2:206824/1‑150 (MQ=255)
                                            cGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCt                                                                                                           >  2:2833878/1‑150 (MQ=255)
                                             gAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTg                                                                                                          >  1:1441252/1‑150 (MQ=255)
                                             gAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTACATCCGCAGAAATAACGTCACTCGTTCGCCACGCATATGCTg                                                                                                          >  1:178966/1‑150 (MQ=255)
                                                gacgacGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCAGATGCTGGAg                                                                                                       >  1:2327467/1‑150 (MQ=255)
                                                 acgacgCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGt                                                                                                      >  2:3462325/1‑150 (MQ=255)
                                                      gcgcTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAgc                                                                                                 <  2:4181347/150‑1 (MQ=255)
                                                      gcgcTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGCCGAgc                                                                                                 <  1:1706761/150‑1 (MQ=255)
                                                        gcTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAgcgg                                                                                               >  1:4175600/1‑150 (MQ=255)
                                                           ttttCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGa                                                                                            >  2:4009374/1‑150 (MQ=255)
                                                                 gTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAGATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCg                                                                                      >  1:4426918/1‑150 (MQ=255)
                                                                 gTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCg                                                                                      >  2:3939105/1‑150 (MQ=255)
                                                                  tCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCgt                                                                                     >  1:2907190/1‑150 (MQ=255)
                                                                  tCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCgt                                                                                     <  2:2210763/150‑1 (MQ=255)
                                                                   cGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCgtg                                                                                    >  1:2638955/1‑150 (MQ=255)
                                                                     aaaCTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGAATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACTCATATGCTGGAGTCGAGCTGCGATCTTCgtggt                                                                                  >  2:684770/1‑150 (MQ=255)
                                                                          gggCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCa                                                                             >  1:4262401/1‑150 (MQ=255)
                                                                           ggCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAg                                                                            <  1:846388/150‑1 (MQ=255)
                                                                             cAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGa                                                                          >  1:32809/1‑150 (MQ=255)
                                                                                    aTCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGctgctg                                                                   >  2:4763040/1‑150 (MQ=255)
                                                                                      ctcCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGTAGGAGCTGCTggg                                                                 >  1:4962055/1‑150 (MQ=255)
                                                                                              gTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCa                                                         >  1:1091754/1‑150 (MQ=255)
                                                                                              gTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCa                                                         >  2:3760685/1‑150 (MQ=255)
                                                                                               tAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCaa                                                        >  2:1563532/1‑150 (MQ=255)
                                                                                               tAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCaa                                                        >  2:4784490/1‑150 (MQ=255)
                                                                                                   gtgCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACctc                                                    <  2:750977/150‑1 (MQ=255)
                                                                                                    tgCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACctct                                                   <  2:59591/150‑1 (MQ=255)
                                                                                                      cAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTcc                                                 >  1:239414/1‑150 (MQ=255)
                                                                                                      cAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTcc                                                 <  1:3470727/150‑1 (MQ=255)
                                                                                                       aGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTcca                                                >  1:1215252/1‑150 (MQ=255)
                                                                                                       aGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTcca                                                >  2:77632/1‑150 (MQ=255)
                                                                                                       aGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTcca                                                >  2:4301615/1‑150 (MQ=255)
                                                                                                         aaaCGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCTTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTccacc                                              >  1:3635195/1‑150 (MQ=255)
                                                                                                         aaaCGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTccacc                                              <  2:2627138/150‑1 (MQ=255)
                                                                                                             gCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGc                                          <  1:359806/150‑1 (MQ=255)
                                                                                                              cTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCa                                         <  2:4969818/150‑1 (MQ=255)
                                                                                                                 tGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAAt                                      >  1:392836/1‑150 (MQ=255)
                                                                                                                  gCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATc                                     <  2:2270973/150‑1 (MQ=255)
                                                                                                                    cGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCta                                   >  1:1534445/1‑150 (MQ=255)
                                                                                                                     gAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCtat                                  >  2:1899381/1‑150 (MQ=255)
                                                                                                                     gAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCtat                                  <  2:1200359/150‑1 (MQ=255)
                                                                                                                       aTGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATAc                                <  2:1451187/150‑1 (MQ=255)
                                                                                                                        tGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACt                               >  2:3617309/1‑150 (MQ=255)
                                                                                                                        tGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACt                               >  2:2940061/1‑150 (MQ=255)
                                                                                                                          gggCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCa                             >  2:1839719/1‑150 (MQ=255)
                                                                                                                            gCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATc                           <  1:2784837/150‑1 (MQ=255)
                                                                                                                              aTAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCtt                         <  1:3046344/150‑1 (MQ=255)
                                                                                                                              aTAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCtt                         <  1:990097/150‑1 (MQ=255)
                                                                                                                               tAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTg                        <  2:3505778/150‑1 (MQ=255)
                                                                                                                                aaaaCAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGa                       >  1:4799146/1‑150 (MQ=255)
                                                                                                                                 aaaCAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGAt                      >  2:4366081/1‑150 (MQ=255)
                                                                                                                                 aaaCAAGGGATGAATAATCACGTTCATCCTCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCTAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGTGTCATGCCAACCTCTCCACAACGCAATTCTATACTCATCTTGAt                      >  1:2722288/1‑150 (MQ=255)
                                                                                                                                   aCAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGAttt                    >  2:1771636/1‑150 (MQ=255)
                                                                                                                                   aCAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGAttt                    <  2:518642/150‑1 (MQ=255)
                                                                                                                                     aaGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTc                  >  2:1093284/1‑150 (MQ=255)
                                                                                                                                     aaGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTc                  >  2:4725962/1‑150 (MQ=255)
                                                                                                                                     aaGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTc                  >  2:49136/1‑150 (MQ=255)
                                                                                                                                      aGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCa                 >  2:4065691/1‑150 (MQ=255)
                                                                                                                                      aGGGCTGAATAATCAAGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCa                 <  2:3061437/150‑1 (MQ=255)
                                                                                                                                       gggCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCaa                >  2:3341748/1‑150 (MQ=255)
                                                                                                                                        ggCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAac               >  2:3140974/1‑150 (MQ=255)
                                                                                                                                        ggCTGAATAATCAAGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGAGTTTCAac               >  2:3213784/1‑150 (MQ=255)
                                                                                                                                         gCTGTATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAaca              <  1:3642814/150‑1 (MQ=255)
                                                                                                                                          cTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAacac             >  1:3838917/1‑150 (MQ=255)
                                                                                                                                            gAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCt           <  1:2833878/150‑1 (MQ=255)
                                                                                                                                            gAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCt           >  2:5107627/1‑150 (MQ=255)
                                                                                                                                            gAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCt           >  1:5135228/1‑150 (MQ=255)
                                                                                                                                                aatCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGcc       >  1:4656080/1‑150 (MQ=255)
                                                                                                                                                 atCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCt      >  1:542460/1‑150 (MQ=255)
                                                                                                                                                  tCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCTc     <  2:3320752/150‑1 (MQ=255)
                                                                                                                                                  tCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCTc     <  1:687510/150‑1 (MQ=255)
                                                                                                                                                   cACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCTCg    <  2:1441252/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGTGGATTGAGCACTGGCTTGATTTGCGCGACCTGTTTGGTAGCGAAGACGACGCGCTTTTTCTGTCGAAACTGGGCAAGCGTATCTCCGCGCGTAATGTGCAGAAACGCTTTGCCGAATGGGGCATAAAACAAGGGCTGAATAATCACGTTCATCCGCATAAATTACGTCACTCGTTCGCCACGCATATGCTGGAGTCGAGCGGCGATCTTCGTGGTGTGCAGGAGCTGCTGGGTCATGCCAACCTCTCCACCACGCAAATCTATACTCATCTTGATTTTCAACACCTTGCCTCGGT  >  CP009273/3990205‑3990503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑