Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,994,076 A→T 100% L114* (TTA→TAA)  yigE ← DUF2233 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,994,0760AT100.0% 1004.5 / NA 298L114* (TTA→TAA) yigEDUF2233 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (160/138);  total (160/138)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCC  >  CP009273/3993929‑3994221
                                                                                                                                                   |                                                                                                                                                 
aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTAc                                                                                                                                                 >  1:2701476/1‑150 (MQ=255)
aTCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTAc                                                                                                                                                 >  1:2578439/1‑150 (MQ=255)
  cAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGc                                                                                                                                               >  1:4502723/1‑150 (MQ=255)
  cAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGc                                                                                                                                               >  1:642428/1‑150 (MQ=255)
      aTTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCAcc                                                                                                                                           >  2:2523588/1‑150 (MQ=255)
          gCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTct                                                                                                                                       <  1:1352255/150‑1 (MQ=255)
          gCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTct                                                                                                                                       <  1:2348807/150‑1 (MQ=255)
               gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGACATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGa                                                                                                                                  >  2:5222725/1‑150 (MQ=255)
               gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGa                                                                                                                                  >  2:1204802/1‑150 (MQ=255)
                aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGAc                                                                                                                                 >  1:3364848/1‑150 (MQ=255)
                     aCCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGttt                                                                                                                            <  1:484877/150‑1 (MQ=255)
                     aCCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGttt                                                                                                                            >  2:5287442/1‑150 (MQ=255)
                          aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGAt                                                                                                                       <  1:4479573/150‑1 (MQ=255)
                           aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATg                                                                                                                      <  1:551359/150‑1 (MQ=255)
                           aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAGTTTACGCCACCTTCTGCTGACCGTTTTCGagg                                                                                                                      >  2:420105/1‑148 (MQ=255)
                            aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGt                                                                                                                     >  1:5309254/1‑150 (MQ=255)
                            aCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGt                                                                                                                     >  2:904929/1‑150 (MQ=255)
                                aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                                                                                                                 >  1:1740187/1‑150 (MQ=255)
                                 aTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa                                                                                                                >  1:2217289/1‑150 (MQ=255)
                                    tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg                                                                                                             >  2:1607997/1‑150 (MQ=255)
                                       ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc                                                                                                          <  1:1799016/150‑1 (MQ=255)
                                        tACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg                                                                                                         >  2:1534385/1‑150 (MQ=255)
                                         aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg                                                                                                        <  1:4634191/150‑1 (MQ=255)
                                         aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg                                                                                                        <  1:747959/150‑1 (MQ=255)
                                         aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcgg                                                                                                        <  1:1978974/150‑1 (MQ=255)
                                          cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc                                                                                                       <  1:3213030/150‑1 (MQ=255)
                                            ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgc                                                                                                     >  2:768305/1‑150 (MQ=255)
                                                  gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCt                                                                                               >  1:1232315/1‑150 (MQ=255)
                                                  gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCt                                                                                               >  1:1009241/1‑150 (MQ=255)
                                                    aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCttt                                                                                             >  1:3178817/1‑150 (MQ=255)
                                                    aGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTGTTCGATGTACAAACCGAGCGGCGCATAGCttt                                                                                             >  2:2349884/1‑150 (MQ=255)
                                                        aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatc                                                                                         >  1:5077016/1‑150 (MQ=255)
                                                         tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTtcatca                                                                                        >  2:3926240/1‑150 (MQ=255)
                                                              acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGAt                                                                                   <  1:1564461/150‑1 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                 <  1:3756019/150‑1 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                 >  2:1059614/1‑150 (MQ=255)
                                                                gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgc                                                                                 >  2:1672244/1‑150 (MQ=255)
                                                                  acgaTGCCGACTTTATCTCCCGCGACATACAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccg                                                                               <  2:1935469/150‑1 (MQ=255)
                                                                  acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATgccg                                                                               <  2:1513469/150‑1 (MQ=255)
                                                                        ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTc                                                                         <  2:3878566/150‑1 (MQ=255)
                                                                         cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                        <  1:983089/150‑1 (MQ=255)
                                                                         cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                        >  1:4928403/1‑150 (MQ=255)
                                                                         cGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCa                                                                        >  1:3475783/1‑150 (MQ=255)
                                                                           aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATc                                                                      <  2:215484/150‑1 (MQ=255)
                                                                           aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATc                                                                      <  1:1522104/150‑1 (MQ=255)
                                                                                 tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATc                                                                <  2:3596119/150‑1 (MQ=255)
                                                                                  ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCt                                                               <  2:594162/150‑1 (MQ=255)
                                                                                      cgcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAc                                                           <  2:266215/150‑1 (MQ=255)
                                                                                       gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcc                                                          >  1:4366991/1‑150 (MQ=255)
                                                                                        cgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcct                                                         >  2:3482460/1‑150 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCNATCTGCAcctg                                                        <  1:2758185/150‑1 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                        >  2:1168988/1‑150 (MQ=255)
                                                                                         gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctg                                                        <  1:1068630/150‑1 (MQ=255)
                                                                                          aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctga                                                       >  1:4512638/1‑150 (MQ=255)
                                                                                           cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgac                                                      >  1:1481424/1‑150 (MQ=255)
                                                                                              aaaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCGTAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccct                                                   <  2:440634/150‑1 (MQ=255)
                                                                                               aaaCACGCCGCCAGGACGGATAAAGAACTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGGTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctg                                                  >  1:5032987/1‑150 (MQ=255)
                                                                                               aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctg                                                  <  1:909872/150‑1 (MQ=255)
                                                                                               aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCAcctgaccctg                                                  >  2:1722097/1‑150 (MQ=255)
                                                                                                    cgccgcCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAt                                             <  2:3758055/150‑1 (MQ=255)
                                                                                                     gccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTAtt                                            <  1:162908/150‑1 (MQ=255)
                                                                                                      ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTa                                           <  2:5076960/150‑1 (MQ=255)
                                                                                                          cAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTTatat                                       <  1:2522253/150‑1 (MQ=255)
                                                                                                           aGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATc                                      <  1:243260/150‑1 (MQ=255)
                                                                                                            ggACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATcc                                     >  1:5259043/1‑150 (MQ=255)
                                                                                                            ggACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATcc                                     <  1:2295179/150‑1 (MQ=255)
                                                                                                            ggACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATcc                                     <  2:1114709/150‑1 (MQ=255)
                                                                                                             gACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCg                                    <  2:2919748/150‑1 (MQ=255)
                                                                                                               cGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGcc                                  <  2:3364848/150‑1 (MQ=255)
                                                                                                                ggATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCca                                 >  1:4405690/1‑150 (MQ=255)
                                                                                                                ggATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCca                                 >  1:565320/1‑150 (MQ=255)
                                                                                                                 gATAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcag                                >  1:1909958/1‑150 (MQ=255)
                                                                                                                   tAAAGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagca                              >  2:133628/1‑150 (MQ=255)
                                                                                                                    aaaGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagcag                             >  2:40682/1‑150 (MQ=255)
                                                                                                                    aaaGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagcag                             <  1:858637/150‑1 (MQ=255)
                                                                                                                    aaaGAAATTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCcagcag                             >  2:4431397/1‑150 (MQ=255)
                                                                                                                         aaTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCAt                        >  1:4763137/1‑150 (MQ=255)
                                                                                                                          aTTCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATg                       >  1:2004740/1‑150 (MQ=255)
                                                                                                                            tCCCTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTa                     >  2:4190159/1‑150 (MQ=255)
                                                                                                                             cccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTaa                    >  1:3638538/1‑150 (MQ=255)
                                                                                                                             cccTTCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTaa                    >  2:2073665/1‑150 (MQ=255)
                                                                                                                                 tCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGtt                <  2:1452409/150‑1 (MQ=255)
                                                                                                                                 tCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGtt                <  1:1177588/150‑1 (MQ=255)
                                                                                                                                 tCACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGtt                >  2:4883684/1‑150 (MQ=255)
                                                                                                                                  cACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTc               >  2:3196938/1‑150 (MQ=255)
                                                                                                                                  cACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTc               <  2:4516918/150‑1 (MQ=255)
                                                                                                                                  cACCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTc               <  1:122324/150‑1 (MQ=255)
                                                                                                                                   aCCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcc              <  2:1817850/150‑1 (MQ=255)
                                                                                                                                   aCCTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcc              >  2:136830/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCTGCCAGCAGAGCATGTAACGTTccc             >  1:5117638/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTccc             >  2:127618/1‑150 (MQ=255)
                                                                                                                                     cTGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTcccc            <  1:4168025/150‑1 (MQ=255)
                                                                                                                                      tGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCa           <  2:3112872/150‑1 (MQ=255)
                                                                                                                                      tGAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCa           >  1:4321219/1‑150 (MQ=255)
                                                                                                                                       gAAGCGAGATTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCAc          >  1:299041/1‑150 (MQ=255)
                                                                                                                                            gagaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTc     >  2:2833635/1‑150 (MQ=255)
                                                                                                                                            gagaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTc     <  2:1365203/150‑1 (MQ=255)
                                                                                                                                             agaTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCg    >  2:4812346/1‑150 (MQ=255)
                                                                                                                                               aTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGcc  <  1:2067118/150‑1 (MQ=255)
                                                                                                                                               aTTTTACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGcc  >  2:147922/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATCAACATTGGCCCTGACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTCATCATAGATGCCGCCGTTCATCGCCATCTGCACCTGACCCTGACTATTAATATCCGCCAGCAGAGCATGTAACGTTCCCCACGCTTCGCC  >  CP009273/3993929‑3994221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑