BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,001,612	G→T	6.1%	V166F (GTC→TTC)	rhtC →	threonine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,001,612	0	G	T	6.1%	964.3 / 23.5	259	V166F (GTC→TTC)	rhtC	threonine efflux pump
Reads supporting (aligned to +/- strand): ref base G (119/124); new base T (11/5); total (130/129)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 269 total aligned reads displayed.

TGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAG  >  CP009273/4001463‑4001761

 gAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACcgt                                                                                                                                                      >  2:4686431/1‑150 (MQ=255)

 gAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACcgt                                                                                                                                                      >  1:1307939/1‑150 (MQ=255)

        ttACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGGTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGcc                                                                                                                                               >  1:1655001/1‑150 (MQ=255)

         tACTTACAAATATCGCTAATCCGTATGGTATTATCTACTTTGGTTATGTGTTATCATTGTTTGTCGGTGATAACTAGGGCACTTGCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCa                                                                                                                                              <  1:4876830/150‑1 (MQ=255)

            tGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGcc                                                                                                                                           >  2:465924/1‑150 (MQ=255)

            tGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGcc                                                                                                                                           >  1:495687/1‑150 (MQ=255)

             gACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCt                                                                                                                                          >  1:1603646/1‑150 (MQ=255)

               ccAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGt                                                                                                                                        >  2:2221545/1‑150 (MQ=255)

                 aaTCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGttt                                                                                                                                      <  2:5189521/150‑1 (MQ=255)

                 aaTCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGttt                                                                                                                                      <  2:4346593/150‑1 (MQ=255)

                    ctctctAATCCGACATCGTTTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATCACGTTGGCACTACCGCGCGCTGGGGCATTTTTTCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGcc                                                                                                                                   <  1:1057593/150‑1 (MQ=255)

                    ctcGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGcc                                                                                                                                   >  1:3304940/1‑150 (MQ=255)

                     tcGCTAAGCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGccc                                                                                                                                  <  2:3411723/150‑1 (MQ=255)

                      cGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCt                                                                                                                                 <  1:3650154/150‑1 (MQ=255)

                      cGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCt                                                                                                                                 >  2:18056/1‑150 (MQ=255)

                       gCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTg                                                                                                                                <  1:2472207/150‑1 (MQ=255)

                           aTCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGc                                                                                                                            <  2:5207961/150‑1 (MQ=255)

                             ccGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCaa                                                                                                                          <  2:758008/150‑1 (MQ=255)

                               gAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTTCCCTGCCGCAAAt                                                                                                                        >  2:1653631/1‑150 (MQ=255)

                               gAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAAt                                                                                                                        <  2:1406371/150‑1 (MQ=255)

                                 aaGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATgc                                                                                                                      >  1:54795/1‑150 (MQ=255)

                                     gATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCg                                                                                                                  >  2:4204845/1‑150 (MQ=255)

                                     gATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCg                                                                                                                  >  2:1869082/1‑150 (MQ=255)

                                      aTTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCCGCCTGTTTGCCCTGCCGCAAATGCGCCGt                                                                                                                 >  2:3081309/1‑150 (MQ=255)

                                      aTTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGt                                                                                                                 >  1:1845577/1‑150 (MQ=255)

                                           cTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTa                                                                                                            <  1:5098816/150‑1 (MQ=255)

                                           cTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTa                                                                                                            <  1:778670/150‑1 (MQ=255)

                                             aCTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATc                                                                                                          >  1:894319/1‑150 (MQ=255)

                                              cTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCa                                                                                                         >  1:4980392/1‑150 (MQ=255)

                                               tttGGCTCGGTGTTCTCATTGTTTGTAGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCaa                                                                                                        <  2:2365823/150‑1 (MQ=255)

                                                ttGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAAc                                                                                                       <  2:61248/150‑1 (MQ=255)

                                                  ggCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCAGTTTTCCCAGCCGCAAATGCGCCGGGGTTATAAAAGt                                                                                                     >  1:3624134/1‑150 (MQ=255)

                                                   gCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGta                                                                                                    >  2:1574296/1‑149 (MQ=255)

                                                    cTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCCGCCTGTTTTACCAGCCCCAAAAGCTCCGTGGTTATCAAcctat                                                                                                   >  1:1870156/1‑146 (MQ=255)

                                                     tCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTg                                                                                                  <  2:4612354/150‑1 (MQ=255)

                                                         tgtTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATAAACGTCTGGCGa                                                                                              >  1:108835/1‑150 (MQ=255)

                                                           ttCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAAAGCGCCGTGGTTATCAACGTCTGGCGAAg                                                                                            >  2:4228533/1‑150 (MQ=255)

                                                                aTTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAt                                                                                       <  2:4880660/150‑1 (MQ=255)

                                                                 ttgtttgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAtt                                                                                      <  1:4060284/150‑1 (MQ=255)

                                                                 ttgtttgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAtt                                                                                      <  2:4787014/150‑1 (MQ=255)

                                                                 ttgtttgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAtt                                                                                      <  1:5181631/150‑1 (MQ=255)

                                                                  tgtttgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAGTg                                                                                     >  2:1264326/1‑150 (MQ=255)

                                                                      tgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATgg                                                                                 <  1:2060794/150‑1 (MQ=255)

                                                                      tgtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCAGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGGTTTATgg                                                                                 >  2:1035018/1‑150 (MQ=255)

                                                                       gtCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGt                                                                                >  2:3533914/1‑150 (MQ=255)

                                                                          ggTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTTCCCGGCCGCAAATGCGCCGGGGTTATCAACGTCTGGCGGAGTGGGGTGGTGGgttt                                                                             >  1:2318413/1‑150 (MQ=255)

                                                                          ggTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGtttt                                                                             <  1:5058660/150‑1 (MQ=255)

                                                                           gTGATAAGGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGTAACGCTGGCGTGGTTTACCGTCGGTGCCAGCCTGTGTGCCCTGGCGCAATTGCGCCGTGGTTATCAACGTCTGGCGATGTGGATTGATGGTTTTg                                                                            <  2:3038012/150‑1 (MQ=255)

                                                                           gTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTg                                                                            >  1:4208556/1‑150 (MQ=255)

                                                                             gATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGcc                                                                          >  2:3853474/1‑150 (MQ=255)

                                                                             gATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGcc                                                                          <  2:538443/150‑1 (MQ=255)

                                                                             gATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGAGTGATGGTTTTGcc                                                                          >  2:603187/1‑150 (MQ=255)

                                                                              aTAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCg                                                                         <  1:2108743/150‑1 (MQ=255)

                                                                              aTAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCg                                                                         >  2:2159905/1‑150 (MQ=255)

                                                                                aaCGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCggg                                                                       >  1:2233259/1‑150 (MQ=255)

                                                                                  cGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGc                                                                     <  1:5131043/150‑1 (MQ=255)

                                                                                   gTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCg                                                                    >  1:2906805/1‑150 (MQ=255)

                                                                                       gCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTAt                                                                <  1:43803/150‑1 (MQ=255)

                                                                                         aCTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTAttt                                                              <  2:1488580/150‑1 (MQ=255)

                                                                                          cTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTg                                                             >  2:713349/1‑150 (MQ=255)

                                                                                          cTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGGTTTGCCGGGGCGTTATTTg                                                             >  2:3313884/1‑150 (MQ=255)

                                                                                           tACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGc                                                            >  1:4838007/1‑150 (MQ=255)

                                                                                                 gcgcTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGAtt                                                      <  2:2046299/150‑1 (MQ=255)

                                                                                                  cgcTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGAttt                                                     <  1:1789230/150‑1 (MQ=255)

                                                                                                    cTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCAGCCCCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTTATGGTTTTGCCGGGGCGTTAATTGCCGGATTTgg                                                   >  2:1922810/1‑150 (MQ=255)

                                                                                                     tGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTTCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGc                                                  >  2:5244977/1‑150 (MQ=255)

                                                                                                     tGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGc                                                  >  2:778816/1‑150 (MQ=255)

                                                                                                      ggggCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGTTGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGca                                                 >  2:452889/1‑150 (MQ=255)

                                                                                                       gggCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGcat                                                <  1:456741/150‑1 (MQ=255)

                                                                                                       gggCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGcat                                                <  2:2674373/150‑1 (MQ=255)

                                                                                                        ggCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATAAAAGTCTGGCGAAGTGGATTGATGGTTTTGGCGGGGCGTTATTTGACGGATTTTGcatt                                               >  2:5005768/1‑150 (MQ=255)

                                                                                                          cATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCTTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGcattca                                             >  2:3072405/1‑150 (MQ=255)

                                                                                                          cATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGcattca                                             >  2:4834247/1‑150 (MQ=255)

                                                                                                             ttttGCGCTGATCATTGTCGACACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCAttt                                          <  2:2936633/150‑1 (MQ=255)

                                                                                                              tttGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTg                                         <  1:3839947/150‑1 (MQ=255)

                                                                                                              tttGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTg                                         <  2:2730262/150‑1 (MQ=255)

                                                                                                               ttGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCAGTTTGCCCGGCCGCAAATGCGCCGGGGGTATCAACGTCTGGCGAAGTGGGTTGATGGTTTTGCCGGGGGGTGTTTTTGCGGGTTTGGCATTCATTTgg                                        >  1:1532474/1‑149 (MQ=255)

                                                                                                                 gcgcTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATAGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGatt                                      <  2:85260/150‑1 (MQ=255)

                                                                                                                  cgcTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTTGCATTCATTTGatta                                     >  2:1760851/1‑150 (MQ=255)

                                                                                                                  cgcTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGatta                                     <  1:237705/150‑1 (MQ=255)

                                                                                                                  cgcTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGatta                                     <  1:1988347/150‑1 (MQ=255)

                                                                                                                  cgcTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGGGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGatta                                     >  1:371428/1‑150 (MQ=255)

                                                                                                                     tGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGGCAGGCGGTTTTCCCAGCCGCAAATGCGCCGGGGTTATAAACCTCTGGCGAAGTGGGTTGGTGGGTTTGCCGGGGCGGTATTTGGCGGGGTTTGGAGTGATTTGTTTAttt                                  >  1:1359919/1‑150 (MQ=255)

                                                                                                                       aTCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTTCCCTGCCGCAAATGCGCCGTGGTTATAAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTcg                                >  2:5064008/1‑150 (MQ=255)

                                                                                                                           ttGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGt                            <  1:1293821/150‑1 (MQ=255)

                                                                                                                           ttGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGt                            <  2:3747376/150‑1 (MQ=255)

                                                                                                                              tCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGAt                         <  1:3984262/150‑1 (MQ=255)

                                                                                                                              tCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGAt                         <  2:1649597/150‑1 (MQ=255)

                                                                                                                                gAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTACCGGGGCGTTATTTGCCGGATTTGGCCTTCATTTGATTATTTCGCGGTGTTGc                       >  1:3546930/1‑150 (MQ=255)

                                                                                                                                     gCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGAc                  <  2:1135681/150‑1 (MQ=255)

                                                                                                                                      cTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTTTCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGAcg                 >  2:558817/1‑150 (MQ=255)

                                                                                                                                      cTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGAcg                 <  1:3080368/150‑1 (MQ=255)

                                                                                                                                         gCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTTGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGt              <  1:3207595/150‑1 (MQ=255)

                                                                                                                                           gTGGTTTACCGTCGTTTCCAGCCAGTTTGCCCTGCCGCAAAATCGCCGGGGTTATAAACCTATGGCGAATTTGATTGATGGTTTTGCCGGGGCGGTATTTGCCGGAATTTGCATTCCTTTTATTTTTTAGCGGTGATGCCCGACGCGTCt            >  1:4778185/1‑150 (MQ=255)

                                                                                                                                            tGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCtt           <  1:3449495/150‑1 (MQ=255)

                                                                                                                                             ggTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTc          <  1:4336517/150‑1 (MQ=255)

                                                                                                                                                ttACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGGAGTGGATTGATGGGTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGGCTTCaga       >  1:2673806/1‑150 (MQ=255)

                                                                                                                                                 tACCGTCGTTGCCAGCCTGTTTGCCCAGCCGCAAAATCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTTGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCagag      >  1:2930555/1‑150 (MQ=255)

                                                                                                                                                  aCCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGt     >  2:3475168/1‑150 (MQ=255)

                                                                                                                                                    cgtcgtTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTaa   >  2:3926423/1‑150 (MQ=255)

TGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAG  >  CP009273/4001463‑4001761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`