Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,020,049 C→G 28.8% P55A (CCG→GCG)  fre → NAD(P)H‑flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,020,0490CG28.8% 686.2 / inf 343P55A (CCG→GCG) freNAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand):  ref base C (115/129);  new base G (38/61);  total (153/190)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 346 total aligned reads displayed.

GCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGT  >  CP009273/4019900‑4020198
                                                                                                                                                     |                                                                                                                                                     
   gTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCgg                                                                                                                                                    >  1:5184874/1‑150 (MQ=255)
    tAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGa                                                                                                                                                   <  1:5001176/150‑1 (MQ=255)
     aaaGTGACCTCGGTAGAAGCTATCTCTGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCATTTTGTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGAt                                                                                                                                                  <  1:4280185/150‑1 (MQ=255)
      aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATg                                                                                                                                                 <  2:533169/150‑1 (MQ=255)
      aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATg                                                                                                                                                 <  1:4656324/150‑1 (MQ=255)
             cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAgg                                                                                                                                          >  1:3271406/1‑150 (MQ=255)
             cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAgg                                                                                                                                          <  1:4457470/150‑1 (MQ=255)
              tCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAggg                                                                                                                                         <  2:2709108/150‑1 (MQ=255)
               cGGTAGAAGCTATCACGGATACCGTTTATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGt                                                                                                                                        <  2:1642928/150‑1 (MQ=255)
               cGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGt                                                                                                                                        <  2:1750560/150‑1 (MQ=255)
                ggTAGAAGCTATCCCGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGtt                                                                                                                                       <  1:4815382/150‑1 (MQ=255)
                     aaGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCg                                                                                                                                  >  2:3885711/1‑150 (MQ=255)
                       gCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCTGTATTTGATGGTTGTGTTGGATGTGCGCTAAAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAg                                                                                                                                <  1:2281240/150‑1 (MQ=255)
                        cTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTAGTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGc                                                                                                                               >  2:3064321/1‑150 (MQ=255)
                         tATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                                                                                              >  2:3974403/1‑150 (MQ=255)
                         tATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                                                                                              >  1:3916950/1‑150 (MQ=255)
                         tATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt                                                                                                                              >  1:1336788/1‑150 (MQ=255)
                            cACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCa                                                                                                                           <  2:4977868/150‑1 (MQ=255)
                              cGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                                                                                         <  2:2439636/150‑1 (MQ=255)
                              cGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                                                                                         <  2:4859171/150‑1 (MQ=255)
                              cGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCata                                                                                                                         <  1:4837596/150‑1 (MQ=255)
                               ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCatat                                                                                                                        <  2:201237/150‑1 (MQ=255)
                               ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCatat                                                                                                                        <  2:4082026/150‑1 (MQ=255)
                                gATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATAtt                                                                                                                       <  1:4132703/150‑1 (MQ=255)
                                gATACCGTATATCGTGTCCGCATCCTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAATGGTTTATCGAGCTGCATAtt                                                                                                                       >  2:840609/1‑150 (MQ=255)
                                 aTACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTg                                                                                                                      <  1:2677465/150‑1 (MQ=255)
                                 aTACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCAGATTg                                                                                                                      >  1:2517699/1‑150 (MQ=255)
                                     cGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGgcgc                                                                                                                  >  2:4044416/1‑150 (MQ=255)
                                      gTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCAATACGTGCAGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAAAGTCCGTTCTCAAAGGCATCGACGCCGGAAGAAAAAGGGTTTATCTAGCTGCATATTGGCGCt                                                                                                                 >  1:4352974/1‑150 (MQ=255)
                                       tataTCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCtt                                                                                                                <  1:560551/150‑1 (MQ=255)
                                        atatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTc                                                                                                               <  1:786913/150‑1 (MQ=255)
                                           tCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTACATATTGGCGCTTCTGa                                                                                                            <  1:3849148/150‑1 (MQ=255)
                                              tgtCCGCATCGTGCCATACGCGGCCTTTTATTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCTACAAACGTCAGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAt                                                                                                         <  1:1264508/150‑1 (MQ=255)
                                              tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTTATGGTCAGTCTTTGATGGTATTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGTCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAt                                                                                                         >  2:4277570/1‑150 (MQ=255)
                                              tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAt                                                                                                         <  2:3662526/150‑1 (MQ=255)
                                              tgtCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAAt                                                                                                         <  2:698744/150‑1 (MQ=255)
                                                tCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGGATATTGGCGCTTCTGAAATCa                                                                                                       >  1:604804/1‑150 (MQ=255)
                                                  cGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAAc                                                                                                     <  1:4241602/150‑1 (MQ=255)
                                                  cGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAAc                                                                                                     >  1:1148249/1‑150 (MQ=255)
                                                     aTCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGGTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCtt                                                                                                  >  2:4516065/1‑150 (MQ=255)
                                                      tCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCttt                                                                                                 <  1:4054331/150‑1 (MQ=255)
                                                       cGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGACAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTa                                                                                                <  2:5152060/150‑1 (MQ=255)
                                                        gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAc                                                                                               <  1:4573952/150‑1 (MQ=255)
                                                        gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAc                                                                                               <  2:4650508/150‑1 (MQ=255)
                                                        gTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAc                                                                                               <  1:1128619/150‑1 (MQ=255)
                                                          gCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTAcgc                                                                                             >  2:3624718/1‑150 (MQ=255)
                                                              gACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGaaa                                                                                         >  2:2518231/1‑150 (MQ=255)
                                                              gACGCGGCATTTTCTTTTCGTGCTGGTCAGTATTTGATGGTTGTGATGGATGAGCGCGACAATCGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGaaa                                                                                         <  2:2962492/150‑1 (MQ=255)
                                                               aCGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAg                                                                                        <  1:3044278/150‑1 (MQ=255)
                                                                   ggCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGt                                                                                    >  2:3194265/1‑150 (MQ=255)
                                                                    gCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc                                                                                   >  1:3323029/1‑150 (MQ=255)
                                                                    gCCATTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc                                                                                   <  2:2794926/150‑1 (MQ=255)
                                                                     ccTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa                                                                                  <  1:2933944/150‑1 (MQ=255)
                                                                     ccTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa                                                                                  >  1:929450/1‑150 (MQ=255)
                                                                     ccTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCACATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCa                                                                                  <  2:2872347/150‑1 (MQ=255)
                                                                      cttttcttttCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCAt                                                                                 <  1:2594142/150‑1 (MQ=255)
                                                                       ttttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATg                                                                                <  1:3552019/150‑1 (MQ=255)
                                                                       ttttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATg                                                                                >  1:494608/1‑150 (MQ=255)
                                                                       ttttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATg                                                                                <  2:1905760/150‑1 (MQ=255)
                                                                        tttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATgg                                                                               >  1:682218/1‑150 (MQ=255)
                                                                        tttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATgg                                                                               <  1:313732/150‑1 (MQ=255)
                                                                          tcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGAc                                                                             <  2:2077651/150‑1 (MQ=255)
                                                                              ttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa                                                                         >  1:1023368/1‑150 (MQ=255)
                                                                              ttcGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCa                                                                         >  1:3695923/1‑150 (MQ=255)
                                                                                  tGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCt                                                                     >  1:1568575/1‑150 (MQ=255)
                                                                                  tGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCt                                                                     <  1:833166/150‑1 (MQ=255)
                                                                                       gTCAGTTTTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGTTGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGTAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCTAAg                                                                <  2:2392576/150‑1 (MQ=255)
                                                                                       gTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAg                                                                <  1:3621398/150‑1 (MQ=255)
                                                                                         cAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGat                                                              <  2:3759895/150‑1 (MQ=255)
                                                                                         cAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGat                                                              <  2:1397243/150‑1 (MQ=255)
                                                                                          aGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatc                                                             <  1:733944/150‑1 (MQ=255)
                                                                                           gTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatca                                                            <  2:649111/150‑1 (MQ=255)
                                                                                            tATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatcat                                                           <  2:2252720/150‑1 (MQ=255)
                                                                                            tATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatcat                                                           <  1:2135169/150‑1 (MQ=255)
                                                                                             aTTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatcatc                                                          <  2:1546394/150‑1 (MQ=255)
                                                                                             aTTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGatcatc                                                          >  2:502999/1‑150 (MQ=255)
                                                                                               atGATGGTAGTGATGGATGAGCGCGACAAACGACCGTTCTCAATGGCTACGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCTAAAGCAGTCATGGACCGCATCCTCAAAGATCATCaa                                                        >  2:109185/2‑150 (MQ=255)
                                                                                                 gATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCACTGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAAt                                                      <  2:1049025/150‑1 (MQ=255)
                                                                                                 gATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAAt                                                      >  1:442841/1‑150 (MQ=255)
                                                                                                 gATGGTAGTGAGGGATGAGCGCGACAAACGTCCGGTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAAt                                                      <  1:5284330/150‑1 (MQ=255)
                                                                                                  aTGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATc                                                     <  2:763044/150‑1 (MQ=255)
                                                                                                   tGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCg                                                    >  2:4202178/1‑150 (MQ=255)
                                                                                                      tAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTgg                                                 >  1:4763000/1‑150 (MQ=255)
                                                                                                       aGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGt                                                >  1:197365/1‑150 (MQ=255)
                                                                                                       aGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGt                                                >  1:3182815/1‑150 (MQ=255)
                                                                                                        gTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTc                                               >  1:149087/1‑150 (MQ=255)
                                                                                                              gatgAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAtt                                         <  1:1790881/150‑1 (MQ=255)
                                                                                                                  aGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcccc                                     <  2:3293784/150‑1 (MQ=255)
                                                                                                                   gcgcGACAAACGTCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCa                                    <  1:2419031/150‑1 (MQ=255)
                                                                                                                         cAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGgaga                              >  1:2381020/1‑150 (MQ=255)
                                                                                                                           aaCGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAg                            >  2:1235072/1‑150 (MQ=255)
                                                                                                                               tCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTg                        <  1:1851140/150‑1 (MQ=255)
                                                                                                                               tCCGTTCTCAATGGCTTCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTg                        >  1:2821568/1‑150 (MQ=255)
                                                                                                                               tCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACACGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTg                        >  2:2382372/1‑150 (MQ=255)
                                                                                                                                ccGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTgg                       >  2:2018339/1‑150 (MQ=255)
                                                                                                                                  gTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCt                     <  1:4045181/150‑1 (MQ=255)
                                                                                                                                    tctcAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc                   >  2:4551204/1‑150 (MQ=255)
                                                                                                                                       cAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATACCCCACGGAGAAGCGTGGCTGcgcg                >  2:3228914/1‑150 (MQ=255)
                                                                                                                                            gCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCgatgat           >  2:1121104/1‑150 (MQ=255)
                                                                                                                                               tCGACGGCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGaa        <  2:4817971/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGT  >  CP009273/4019900‑4020198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑