BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,020,520	G→T	5.4%	D212Y (GAT→TAT)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,020,520	0	G	T	5.4%	1165.7 / 18.8	314	D212Y (GAT→TAT)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base G (138/156); new base T (12/7); total (151/163)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 331 total aligned reads displayed.

TGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTAT  >  CP009273/4020371‑4020669

 gctaGTGGTGCCGGTGGTTGAACAACCGGATGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGa                                                                                                                                                      <  1:697745/147‑1 (MQ=255)

 gCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGa                                                                                                                                                      >  1:1163157/1‑150 (MQ=255)

  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGAt                                                                                                                                                     >  1:3761969/1‑150 (MQ=255)

   aaGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATc                                                                                                                                                    >  2:230593/1‑150 (MQ=255)

    aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCt                                                                                                                                                   <  2:2377116/150‑1 (MQ=255)

    aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCt                                                                                                                                                   <  1:2958195/150‑1 (MQ=255)

     gtggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTg                                                                                                                                                  <  2:2840893/150‑1 (MQ=255)

      tggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGt                                                                                                                                                 >  2:3165848/1‑150 (MQ=255)

       ggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGtt                                                                                                                                                >  2:3442100/1‑150 (MQ=255)

        gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGttt                                                                                                                                               <  2:1550044/150‑1 (MQ=255)

           ccGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGc                                                                                                                                            >  1:445550/1‑150 (MQ=255)

             ggtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAg                                                                                                                                          >  2:877339/1‑150 (MQ=255)

              gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGt                                                                                                                                         <  2:2664184/150‑1 (MQ=255)

               tggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTg                                                                                                                                        <  2:3217172/150‑1 (MQ=255)

               tggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTg                                                                                                                                        <  2:3754112/150‑1 (MQ=255)

               tggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTg                                                                                                                                        <  2:1593909/150‑1 (MQ=255)

                  ttGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGc                                                                                                                                     <  2:1069240/150‑1 (MQ=255)

                   tGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCg                                                                                                                                    <  1:3644370/150‑1 (MQ=255)

                    gAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGt                                                                                                                                   >  2:2105763/1‑150 (MQ=255)

                    gAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGt                                                                                                                                   >  1:4794219/1‑150 (MQ=255)

                       caacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGAAGAGCATGATATCTATATTGCCGGACGGTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGAGAGCGaaaa                                                                                                                                >  2:2582962/1‑149 (MQ=255)

                        aacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATg                                                                                                                               <  1:1355436/150‑1 (MQ=255)

                           cGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATgcgc                                                                                                                            >  2:1760012/1‑150 (MQ=255)

                                  gggCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGa                                                                                                                     >  2:3030578/1‑150 (MQ=255)

                                    gCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATc                                                                                                                   <  2:2001401/150‑1 (MQ=255)

                                    gCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATc                                                                                                                   <  1:3442835/150‑1 (MQ=255)

                                       ggCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGcc                                                                                                                >  2:5066943/1‑150 (MQ=255)

                                        gCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCTAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCt                                                                                                               <  2:4085332/150‑1 (MQ=255)

                                            gggCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGttt                                                                                                           <  1:4905356/150‑1 (MQ=255)

                                             ggCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTg                                                                                                          >  1:373224/1‑150 (MQ=255)

                                               cGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGc                                                                                                        >  1:236525/1‑150 (MQ=255)

                                               cGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGc                                                                                                        >  1:3871771/1‑150 (MQ=255)

                                                  aCTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                     <  1:505580/150‑1 (MQ=255)

                                                  aCTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                     >  2:4762778/1‑150 (MQ=255)

                                                  aCTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGAt                                                                                                     <  2:1190509/150‑1 (MQ=255)

                                                       cACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGtt                                                                                                <  1:1065768/150‑1 (MQ=255)

                                                       cACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGtt                                                                                                >  2:4720126/1‑150 (MQ=255)

                                                        aCCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGttt                                                                                               <  2:3596096/150‑1 (MQ=255)

                                                            tgtTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCAt                                                                                           >  2:1740763/1‑150 (MQ=255)

                                                             gtTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCAtt                                                                                          <  1:4000679/150‑1 (MQ=255)

                                                               tAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTa                                                                                        >  2:2909443/1‑150 (MQ=255)

                                                                aaCGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTAt                                                                                       <  1:883656/150‑1 (MQ=255)

                                                                 aCGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATc                                                                                      <  2:629831/150‑1 (MQ=255)

                                                                      ggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTgaga                                                                                 >  2:5219607/1‑150 (MQ=255)

                                                                        tATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCTCCTGTTTGGCGATGCGTTTGCATTTATCTGAGata                                                                               >  1:4720426/1‑150 (MQ=255)

                                                                        tATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGata                                                                               <  1:339304/150‑1 (MQ=255)

                                                                                 aTCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAAc                                                                      >  1:4507251/1‑150 (MQ=255)

                                                                                  tCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAAcc                                                                     <  2:3871592/150‑1 (MQ=255)

                                                                                      ggTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCTTTTATCTGAGATATAAAAAAACCCGcc                                                                 <  1:3537133/150‑1 (MQ=255)

                                                                                         aCGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGcccca                                                              <  2:4474534/150‑2 (MQ=255)

                                                                                          cGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTg                                                             <  1:3226508/150‑1 (MQ=255)

                                                                                           gCTGGCATAGAATTATTTCTTTATTGCCGGACGTTTTGATATGGCTAAAATTGCCCGCTATCTTTTTTGCTGTGAGCGTAATGCGCGGTAAGATCGCCTGTTTGGCGTTGCGTTTGAATTTATCTGAGATATAAAAAAACCCGCCCCTGa                                                            <  1:4071112/150‑1 (MQ=255)

                                                                                                 agagattGATATCTCTATTGCGGGACGTTTTTATATGGCTAAATTTGCCCGCTCTCTTTTTTGCTTGTTTCGTATTGCGCGGGAAGATCGCCTGTTTGGCGTTGATTTTTCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCg                                                      <  1:1435675/144‑1 (MQ=255)

                                                                                                 agagCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCg                                                      >  2:589617/1‑150 (MQ=255)

                                                                                                     cATGAGATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaa                                                  <  2:5271264/150‑1 (MQ=255)

                                                                                                      aTGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaag                                                 >  1:2642603/1‑150 (MQ=255)

                                                                                                      aTGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaag                                                 >  2:1959339/1‑150 (MQ=255)

                                                                                                      aTGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATAGCCAGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAATACCCGCCCCTGACAGGCGGgaag                                                 >  2:3514210/1‑150 (MQ=255)

                                                                                                        gATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGgaagaa                                               >  1:1254780/1‑150 (MQ=255)

                                                                                                           atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACgg                                            >  2:1027300/1‑150 (MQ=255)

                                                                                                           atCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACgg                                            >  1:2102785/1‑150 (MQ=255)

                                                                                                            tCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGc                                           >  1:3204710/1‑150 (MQ=255)

                                                                                                              tataTTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCaa                                         >  1:2787288/1‑150 (MQ=255)

                                                                                                               atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAc                                        >  2:4553898/1‑150 (MQ=255)

                                                                                                               atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAc                                        >  1:1232732/1‑150 (MQ=255)

                                                                                                               atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAc                                        >  2:4242784/1‑150 (MQ=255)

                                                                                                               atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAc                                        <  1:2464785/150‑1 (MQ=255)

                                                                                                               atatTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAc                                        <  2:1221096/150‑1 (MQ=255)

                                                                                                                 atTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTTCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTa                                      >  1:1922234/1‑150 (MQ=255)

                                                                                                                 atTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTa                                      >  1:4364910/1‑150 (MQ=255)

                                                                                                                  ttGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACCGGCGGGAAGAACGGCAACTaa                                     >  2:2726497/1‑150 (MQ=255)

                                                                                                                  ttGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCTATCAGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTaa                                     >  2:5291662/1‑150 (MQ=255)

                                                                                                                   tGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTaaa                                    <  1:2204060/150‑1 (MQ=255)

                                                                                                                    gCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAAc                                   <  2:748796/150‑1 (MQ=255)

                                                                                                                       ggACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGt                                >  2:4004817/1‑150 (MQ=255)

                                                                                                                        gACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGtt                               >  1:1043542/1‑150 (MQ=255)

                                                                                                                         aCGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTa                              <  2:445550/150‑1 (MQ=255)

                                                                                                                           gTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGGTTGCATTTATCTGAGGTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTagt                            >  1:429098/1‑148 (MQ=255)

                                                                                                                              ttGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAg                         >  1:1810173/1‑150 (MQ=255)

                                                                                                                               tGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGt                        <  1:530801/150‑1 (MQ=255)

                                                                                                                                gagaTGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTg                       >  1:4435928/1‑150 (MQ=255)

                                                                                                                                 agaTGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTgg                      >  2:896706/1‑150 (MQ=255)

                                                                                                                                      gCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt                 <  2:1163101/150‑1 (MQ=255)

                                                                                                                                      gCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt                 <  2:4751596/150‑1 (MQ=255)

                                                                                                                                      gCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt                 >  1:1467922/1‑150 (MQ=255)

                                                                                                                                       cGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTa                >  1:812307/1‑150 (MQ=255)

                                                                                                                                       cGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAGTTa                >  1:2798096/1‑150 (MQ=255)

                                                                                                                                        gAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAg               >  2:4224080/1‑150 (MQ=255)

                                                                                                                                        gAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAATTat               >  1:2255244/1‑149 (MQ=255)

                                                                                                                                          aaaTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAt             >  2:393431/1‑150 (MQ=255)

                                                                                                                                          aaaTTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAt             <  1:3030578/150‑1 (MQ=255)

                                                                                                                                             ttGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTa          <  1:1608217/150‑1 (MQ=255)

                                                                                                                                             ttGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTa          <  1:4245309/150‑1 (MQ=255)

                                                                                                                                                 ccGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGAc      <  1:2055218/150‑1 (MQ=255)

                                                                                                                                                  cgcgATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGACGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACg     >  2:4393590/1‑150 (MQ=255)

                                                                                                                                                  cgcgATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACg     >  2:3574302/1‑150 (MQ=255)

                                                                                                                                                    cgATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTa   >  2:1494947/1‑150 (MQ=255)

                                                                                                                                                     gATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAATCTGTTATTCAGTGGCATTTAGATCTATGACGTAt  >  1:41095/1‑150 (MQ=255)

                                                                                                                                                     gATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTAt  >  1:41663/1‑150 (MQ=255)

                                                                                                                                                     gATCAGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTAt  >  1:100742/1‑150 (MQ=255)

TGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTAT  >  CP009273/4020371‑4020669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`