Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,046,172 A→T 100% K256N (AAA→AAT)  hemN → coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,046,1720AT99.6% 898.3 / ‑4.7 271K256N (AAA→AAT) hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (146/124);  total (146/125)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 273 total aligned reads displayed.

CATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGT  >  CP009273/4046025‑4046318
                                                                                                                                                   |                                                                                                                                                  
 aTCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGAt                                                                                                                                                 >  2:2752676/1‑150 (MQ=255)
  tCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAatgatg                                                                                                                                                <  2:4301963/150‑1 (MQ=255)
    gACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCt                                                                                                                                              <  2:5091629/150‑1 (MQ=255)
    gACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCt                                                                                                                                              >  1:5076211/1‑150 (MQ=255)
       cTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAc                                                                                                                                           <  2:602210/150‑1 (MQ=255)
       cTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAc                                                                                                                                           <  1:628963/150‑1 (MQ=255)
       cTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAc                                                                                                                                           <  1:686033/150‑1 (MQ=255)
        tGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAcc                                                                                                                                          >  2:3051182/1‑150 (MQ=255)
          aTTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTg                                                                                                                                        >  1:1488047/1‑150 (MQ=255)
          aTTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTg                                                                                                                                        <  2:1684778/150‑1 (MQ=255)
           tttACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGc                                                                                                                                       <  2:4670835/150‑1 (MQ=255)
            ttACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGcc                                                                                                                                      >  1:2781882/1‑150 (MQ=255)
                ggCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGt                                                                                                                                  <  2:4480373/150‑1 (MQ=255)
                     gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgca                                                                                                                             <  2:5210415/150‑1 (MQ=255)
                      ccGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcag                                                                                                                            <  2:902686/150‑1 (MQ=255)
                       cGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagc                                                                                                                           >  2:3878413/1‑150 (MQ=255)
                       cGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagc                                                                                                                           >  1:3551167/1‑150 (MQ=255)
                       cGAAACAGACGCCGGAGAGTTTCGCCTTAACCCTGAAACGAGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagc                                                                                                                           >  1:4112007/1‑150 (MQ=255)
                           aCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaaa                                                                                                                       >  2:2928214/1‑150 (MQ=255)
                            cAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaaat                                                                                                                      >  2:246847/1‑149 (MQ=255)
                             aGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                     <  1:1598361/150‑1 (MQ=255)
                              gACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACt                                                                                                                    <  2:1916935/150‑1 (MQ=255)
                                cGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCg                                                                                                                  >  1:486970/1‑150 (MQ=255)
                                    ggAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGatat                                                                                                              >  1:1024834/1‑150 (MQ=255)
                                    ggAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGatat                                                                                                              <  1:2540226/150‑1 (MQ=255)
                                     gagaGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATc                                                                                                             <  1:4335858/150‑1 (MQ=255)
                                      agagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATcc                                                                                                            >  2:2360628/1‑150 (MQ=255)
                                       gagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCt                                                                                                           <  1:5180985/150‑1 (MQ=255)
                                         gTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGc                                                                                                         >  1:2065890/1‑150 (MQ=255)
                                          tttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCa                                                                                                        >  1:5277909/1‑150 (MQ=255)
                                          tttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCa                                                                                                        <  1:1317339/150‑1 (MQ=255)
                                          tttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCa                                                                                                        <  1:3872645/150‑1 (MQ=255)
                                           ttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAg                                                                                                       >  1:84849/1‑150 (MQ=255)
                                           ttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAg                                                                                                       >  2:1707283/1‑150 (MQ=255)
                                            tCGCCTTGACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAgg                                                                                                      <  2:4213876/150‑1 (MQ=255)
                                              gCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGaa                                                                                                    >  1:3893695/1‑150 (MQ=255)
                                                cTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAc                                                                                                  >  1:3761943/1‑150 (MQ=255)
                                                cTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAc                                                                                                  >  1:2340497/1‑150 (MQ=255)
                                                 tttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                                                                 >  1:1906159/1‑150 (MQ=255)
                                                   tACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAt                                                                                               <  1:4524173/150‑1 (MQ=255)
                                                     cccTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCg                                                                                             >  1:4932669/1‑150 (MQ=255)
                                                      ccTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                                                            >  1:2592480/1‑150 (MQ=255)
                                                       cTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAGCGcc                                                                                           >  1:3655640/1‑150 (MQ=255)
                                                        tGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                                                          >  1:4236673/1‑150 (MQ=255)
                                                        tGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                                                          <  2:3457584/150‑1 (MQ=255)
                                                        tGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                                                          <  2:396479/150‑1 (MQ=255)
                                                        tGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                                                          >  1:1653378/1‑150 (MQ=255)
                                                        tGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                                                          <  2:542962/150‑1 (MQ=255)
                                                              gtgtGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGa                                                                                    <  1:1875068/150‑1 (MQ=255)
                                                              gtgtGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGa                                                                                    >  1:443219/1‑150 (MQ=255)
                                                               tgtgGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAc                                                                                   <  2:1715186/150‑1 (MQ=255)
                                                                   gCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCaa                                                                               >  1:1021843/1‑150 (MQ=255)
                                                                      gAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                                                            >  1:1471260/1‑150 (MQ=255)
                                                                         cTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                                                         >  1:2247374/1‑150 (MQ=255)
                                                                            aaCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                                                                      >  2:3819637/1‑150 (MQ=255)
                                                                                ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                  <  2:3173985/150‑1 (MQ=255)
                                                                                ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                  <  2:1282111/150‑1 (MQ=255)
                                                                                 cGATCGTCTGAGTGTGTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAACTCAATGTTGCTGACCTGCGGAGTCCGCAGCACAAACTCGATCTCCTGCGGGAAACCATCGCCTTCCTGACGCCATCGGGCTCTCAGtt                                                                 >  1:4137541/1‑150 (MQ=255)
                                                                                  gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCTGGCTATCAGttt                                                                >  1:238303/1‑150 (MQ=255)
                                                                                    tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAt                                                              <  1:2040748/150‑1 (MQ=255)
                                                                                     cGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                                                             <  2:2014065/150‑1 (MQ=255)
                                                                                     cGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                                                             <  2:1329301/150‑1 (MQ=255)
                                                                                      gTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                                                            >  1:3640886/1‑150 (MQ=255)
                                                                                      gTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                                                            <  1:1809771/150‑1 (MQ=255)
                                                                                        cTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                          >  1:769961/1‑150 (MQ=255)
                                                                                        cTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                          <  1:52342/150‑1 (MQ=255)
                                                                                           aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg                                                       >  1:1140425/1‑150 (MQ=255)
                                                                                             tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGa                                                     <  1:3462738/150‑1 (MQ=255)
                                                                                              gtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt                                                    >  2:841029/1‑150 (MQ=255)
                                                                                                cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGAGATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCa                                                  >  1:216549/1‑150 (MQ=255)
                                                                                                  ttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt                                                <  1:5001329/150‑1 (MQ=255)
                                                                                                    aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt                                              >  2:4874214/1‑150 (MQ=255)
                                                                                                    aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt                                              <  2:5005317/150‑1 (MQ=255)
                                                                                                        aCGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGccc                                          <  2:1024753/150‑1 (MQ=255)
                                                                                                          gcgcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGt                                        >  2:5015683/1‑150 (MQ=255)
                                                                                                            gcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTcc                                      >  1:201020/1‑150 (MQ=255)
                                                                                                              aTCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCgg                                    >  2:2720302/1‑150 (MQ=255)
                                                                                                              aTCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCgg                                    >  1:3543768/1‑150 (MQ=255)
                                                                                                              aTCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCgg                                    >  2:4568181/1‑150 (MQ=255)
                                                                                                                cTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGAt                                  <  1:1707283/150‑1 (MQ=255)
                                                                                                                 tGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATg                                 >  1:4763677/1‑150 (MQ=255)
                                                                                                                  gCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGa                                <  2:3701791/150‑1 (MQ=255)
                                                                                                                      aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                            <  1:2979027/150‑1 (MQ=255)
                                                                                                                      aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                            >  1:1353775/1‑150 (MQ=255)
                                                                                                                       ccATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                           >  1:2005577/1‑150 (MQ=255)
                                                                                                                        cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCt                          >  1:40419/1‑150 (MQ=255)
                                                                                                                        cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGACGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTATATCGGTATGGATCACTTTGCCCGACCGGATGACGAGCt                          >  2:107575/1‑150 (MQ=255)
                                                                                                                         aTTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTg                         <  1:3840327/150‑1 (MQ=255)
                                                                                                                           ttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGc                       >  1:1782301/1‑150 (MQ=255)
                                                                                                                           ttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGc                       >  1:336910/1‑150 (MQ=255)
                                                                                                                                ctgctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTgg                  >  1:5045774/1‑150 (MQ=255)
                                                                                                                                 tgctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGc                 >  2:181708/1‑150 (MQ=255)
                                                                                                                                     cAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAg             >  1:2747190/1‑150 (MQ=255)
                                                                                                                                       gcgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCg           >  1:4912241/1‑150 (MQ=255)
                                                                                                                                        cgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt          >  2:4225561/1‑150 (MQ=255)
                                                                                                                                           aaaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGaa       >  1:2903370/1‑150 (MQ=255)
                                                                                                                                             aaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAgg     >  1:312847/1‑150 (MQ=255)
                                                                                                                                             aaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAgg     >  1:784743/1‑150 (MQ=255)
                                                                                                                                              aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGc    <  1:4630692/150‑1 (MQ=255)
                                                                                                                                               tCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCg   <  2:2813207/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGT  >  CP009273/4046025‑4046318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑