Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,056,018 T→A 100% F138I (TTC→ATC)  yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,056,0180TA100.0% 1161.0 / NA 352F138I (TTC→ATC) yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (170/182);  total (170/182)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 363 total aligned reads displayed.

TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTC  >  CP009273/4055871‑4056164
                                                                                                                                                   |                                                                                                                                                  
   ttGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaa                                                                                                                                               <  2:4040843/150‑1 (MQ=255)
    tGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaaa                                                                                                                                              <  1:367809/150‑1 (MQ=255)
        gacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                          <  1:1745779/150‑1 (MQ=255)
         acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAggg                                                                                                                                         >  2:2541864/1‑150 (MQ=255)
           aaTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCg                                                                                                                                       <  1:2392211/150‑1 (MQ=255)
           aaTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCg                                                                                                                                       >  1:2228029/1‑150 (MQ=255)
            aTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGt                                                                                                                                      >  2:586656/1‑150 (MQ=255)
            aTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGt                                                                                                                                      >  2:1852633/1‑150 (MQ=255)
             tGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTg                                                                                                                                     >  2:50464/1‑150 (MQ=255)
                 ggggTCTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 >  2:642689/1‑150 (MQ=255)
                 ggggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 >  1:1182497/1‑150 (MQ=255)
                 ggggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 <  1:4539480/150‑1 (MQ=255)
                 ggggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 >  2:681973/1‑150 (MQ=255)
                 ggggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 >  2:727133/1‑150 (MQ=255)
                     tATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTc                                                                                                                             <  1:2151307/150‑1 (MQ=255)
                      aTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCg                                                                                                                            >  1:596366/1‑150 (MQ=255)
                      aTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCg                                                                                                                            >  2:2270016/1‑150 (MQ=255)
                       tttgggCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGa                                                                                                                           <  1:2053798/147‑1 (MQ=255)
                        tGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAt                                                                                                                          >  1:903395/1‑150 (MQ=255)
                        tGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAt                                                                                                                          >  2:2407748/1‑150 (MQ=255)
                             cATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgt                                                                                                                     <  1:1870602/150‑1 (MQ=255)
                              aTCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtg                                                                                                                    <  1:4997551/150‑1 (MQ=255)
                              aTCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtg                                                                                                                    >  1:3159001/1‑150 (MQ=255)
                                ccTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtggt                                                                                                                  <  1:4051331/150‑1 (MQ=255)
                                 ctctTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtggtg                                                                                                                 >  2:3723867/1‑150 (MQ=255)
                                    tttGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCa                                                                                                              >  1:1430354/1‑150 (MQ=255)
                                       gTCTGGGCGGTATTTGGCGTTACTTGCGACATGATGAACTGGACGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAttt                                                                                                           <  1:3923336/150‑1 (MQ=255)
                                       gTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAttt                                                                                                           <  1:2915346/150‑1 (MQ=255)
                                         cTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTc                                                                                                         >  2:1613282/1‑150 (MQ=255)
                                            ggCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGc                                                                                                      <  1:32508/150‑1 (MQ=255)
                                                cTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                                  <  2:2251656/150‑1 (MQ=255)
                                                cTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                                  <  2:2050610/150‑1 (MQ=255)
                                                cTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                                  >  2:4413948/1‑150 (MQ=255)
                                                       gCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAt                                                                                           <  2:1906625/150‑1 (MQ=255)
                                                        cGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCATGTCGGTAAGTCGATTGGGCAATAGCGAACAACTAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                          <  2:1123711/150‑1 (MQ=255)
                                                        cGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                          <  1:420649/150‑1 (MQ=255)
                                                         gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         >  2:3085141/1‑150 (MQ=255)
                                                         gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         >  1:2854262/1‑150 (MQ=255)
                                                          ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTa                                                                                        >  2:4656831/1‑150 (MQ=255)
                                                             cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGACTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCt                                                                                     >  1:4882748/1‑150 (MQ=255)
                                                              ttGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTg                                                                                    <  2:3503060/150‑1 (MQ=255)
                                                                 cGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAATCAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGtt                                                                                 <  2:3190220/150‑1 (MQ=255)
                                                                 cGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCGGGtt                                                                                 >  1:779192/1‑150 (MQ=255)
                                                                 cGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGAGTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAGTTACCGGGtt                                                                                 >  1:2082844/1‑150 (MQ=255)
                                                                  gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                <  2:3676144/150‑1 (MQ=255)
                                                                  gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                <  1:697732/150‑1 (MQ=255)
                                                                  gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                <  2:1197635/150‑1 (MQ=255)
                                                                  gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                >  2:2465593/1‑150 (MQ=255)
                                                                   aCATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTg                                                                               <  1:3515912/150‑1 (MQ=255)
                                                                      tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCa                                                                            >  2:3008090/1‑150 (MQ=255)
                                                                      tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCa                                                                            >  2:533773/1‑150 (MQ=255)
                                                                             cTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                     <  2:1554121/150‑1 (MQ=255)
                                                                              tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                    <  1:4332869/150‑1 (MQ=255)
                                                                              tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                    <  2:2694233/150‑1 (MQ=255)
                                                                              tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                    >  2:2170981/1‑150 (MQ=255)
                                                                               ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttat                                                                   <  2:1290303/150‑1 (MQ=255)
                                                                                gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttatt                                                                  <  2:5222277/150‑1 (MQ=255)
                                                                                gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttatt                                                                  <  2:196512/150‑1 (MQ=255)
                                                                                gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttatt                                                                  <  1:27305/150‑1 (MQ=255)
                                                                                  cGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAg                                                                >  2:1492238/1‑150 (MQ=255)
                                                                                   ggTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAgg                                                               <  1:3183005/150‑1 (MQ=255)
                                                                                    gTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGt                                                              >  1:446048/1‑150 (MQ=255)
                                                                                      cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATAACGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTTGTTTGGCAGTGGCTTATTAGGttt                                                            >  2:4154712/1‑150 (MQ=255)
                                                                                        tGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTa                                                          <  1:5309138/150‑1 (MQ=255)
                                                                                          cTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaaa                                                        >  2:2756806/1‑150 (MQ=255)
                                                                                                 cGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCa                                                 >  2:2120643/1‑150 (MQ=255)
                                                                                                  ggTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAt                                                >  1:1235588/1‑150 (MQ=255)
                                                                                                   gTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATc                                               <  2:5097454/150‑1 (MQ=255)
                                                                                                    tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCt                                              <  1:102117/150‑1 (MQ=255)
                                                                                                    tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCt                                              <  2:4195331/150‑1 (MQ=255)
                                                                                                     aaGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTg                                             <  2:4854947/150‑1 (MQ=255)
                                                                                                      aGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTgg                                            >  2:3562663/1‑150 (MQ=255)
                                                                                                          taTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                        >  1:5153109/2‑150 (MQ=255)
                                                                                                          gATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                        >  1:2657391/1‑150 (MQ=255)
                                                                                                          gATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                        <  2:1013932/150‑1 (MQ=255)
                                                                                                           aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                       <  1:2407775/150‑1 (MQ=255)
                                                                                                            ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAt                                      <  2:4117745/150‑1 (MQ=255)
                                                                                                             tGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAtt                                     <  2:1792894/150‑1 (MQ=255)
                                                                                                                gCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTccc                                  <  1:1853249/150‑1 (MQ=255)
                                                                                                                     aGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAtt                             <  2:2832343/150‑1 (MQ=255)
                                                                                                                      tcGAACAACAAGGTCGGTTGTTTGGCTTCATCGTAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtg                            <  1:4657180/149‑1 (MQ=255)
                                                                                                                      gCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtg                            <  1:66752/150‑1 (MQ=255)
                                                                                                                       cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgt                           >  2:3050554/1‑150 (MQ=255)
                                                                                                                       cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgt                           <  1:1627886/150‑1 (MQ=255)
                                                                                                                        gAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgtg                          <  1:1786608/150‑1 (MQ=255)
                                                                                                                         aacaacAAGGTCGGTTGTTTGGCTTCATCGAGACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGa                         >  2:4454904/1‑150 (MQ=255)
                                                                                                                          acaacaAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAt                        <  1:4085636/150‑1 (MQ=255)
                                                                                                                           caacaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAtt                       <  1:549943/150‑1 (MQ=255)
                                                                                                                            aacaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTg                      <  2:871655/150‑1 (MQ=255)
                                                                                                                            aacaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTg                      >  2:3234605/1‑150 (MQ=255)
                                                                                                                               aaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg                   <  2:1737133/150‑1 (MQ=255)
                                                                                                                               aaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg                   <  2:144410/150‑1 (MQ=255)
                                                                                                                                aGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGt                  >  2:5016692/1‑150 (MQ=255)
                                                                                                                                 ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTa                 >  1:3919785/1‑150 (MQ=255)
                                                                                                                                      gttgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATCCCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCat            <  2:332012/150‑1 (MQ=255)
                                                                                                                                      gttgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCat            >  2:1249761/1‑150 (MQ=255)
                                                                                                                                       ttgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatt           <  1:1959304/150‑1 (MQ=255)
                                                                                                                                       ttgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatt           <  1:1296523/150‑1 (MQ=255)
                                                                                                                                        tgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatta          >  1:3418553/1‑150 (MQ=255)
                                                                                                                                             ggCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattattatt     <  1:50464/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTC  >  CP009273/4055871‑4056164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑