Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,080,364 G→T 8.5% M1M (ATG→ATT) † yiiG → DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,080,3640GT 8.5% 791.7 / 4.3 255M1M (ATG→ATT) †yiiGDUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (109/111);  new base T (33/2);  total (142/113)
Fisher's exact test for biased strand distribution p-value = 1.76e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

ATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGC  >  CP009273/4080215‑4080513
                                                                                                                                                     |                                                                                                                                                      
aTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATg                                                                                                                                                        <  1:4507446/150‑1 (MQ=255)
aTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTATTTTTTAATTTTTTATTTTGACAATCGTATATATATATTTTTATTGCAAATAAATg                                                                                                                                                        >  1:2753113/1‑150 (MQ=255)
      ccTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAgg                                                                                                                                                  <  1:5048747/150‑1 (MQ=255)
         gCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAt                                                                                                                                               <  1:739829/150‑1 (MQ=255)
          cGAAATATATTAACTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAtt                                                                                                                                              <  1:3753095/150‑1 (MQ=255)
          cGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAtt                                                                                                                                              <  1:5078514/150‑1 (MQ=255)
           gAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTTTTTTTTTATTTTTTTTTTTGAAAATAGTTTGTTTATATTTATTATGAAAATAAATGAAAAAGAAatt                                                                                                                                             >  1:2411719/1‑150 (MQ=255)
           gAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAttt                                                                                                                                             <  2:2662210/150‑1 (MQ=255)
           gAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTGTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAAttt                                                                                                                                             >  2:163284/1‑150 (MQ=255)
            aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATtta                                                                                                                                            <  1:1672029/150‑1 (MQ=255)
            aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTTTGTTCACTCTCTTATCTATATATTTATAATGCAATTAAATGAAAAGGAATtta                                                                                                                                            >  2:4302778/1‑150 (MQ=255)
             aagatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                                                           <  2:2941192/147‑1 (MQ=255)
             aaTATATTACATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                                                           <  2:1683431/150‑1 (MQ=255)
             aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                                                           >  2:3510120/1‑150 (MQ=255)
             aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTTTTATTTTCAATAAATTATATATATATTTATAATGCAATTTAAATAAAATGAATttat                                                                                                                                           >  2:2342722/1‑150 (MQ=255)
              atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTTTTATGTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGAATttatt                                                                                                                                          >  2:213919/1‑150 (MQ=255)
              atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttatt                                                                                                                                          <  2:3715834/150‑1 (MQ=255)
              atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATTTTAAATCTATTATGTATATATTTATAAGGGAAATAAATTAAAAGGAATttatt                                                                                                                                          >  2:1411139/1‑150 (MQ=255)
              atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATGTTAACTATCTTATCTATATATTTATAAGGCAATTAAATTAAAATGAATttatt                                                                                                                                          >  2:4440846/1‑150 (MQ=255)
                atatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTtattat                                                                                                                                        <  1:4606416/150‑1 (MQ=255)
                atatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTGACTCTCGTATGTATAAATATATAATGCAATTAAATTAAAATGAATttaatta                                                                                                                                        >  2:4246267/1‑146 (MQ=255)
                 tatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATc                                                                                                                                       <  1:156485/150‑1 (MQ=255)
                   ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCtt                                                                                                                                     <  1:1284252/150‑1 (MQ=255)
                     aaaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTcc                                                                                                                                   <  2:4503500/150‑1 (MQ=255)
                        ttGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCt                                                                                                                                <  2:132250/150‑1 (MQ=255)
                         tGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTAATTTATTATTTTGACTCTGTTTTCTTTATATTTATAATGCAATTAAATTTAAAGGAATTTATTATATTACGcaa                                                                                                                               >  2:1921515/1‑148 (MQ=255)
                           cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTTTTATTTTCACTCTCTTATCTATATATTTATAATGCAATTAAATGAAAAGGAATTTATTATATTCCGCtatt                                                                                                                             >  2:683715/1‑150 (MQ=255)
                                cTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTTATTTTTTATTTTAACTAAGTTATCTATTTATTTTTTAGGCCATTAAATTTTAAGGTATTTTTTTTATTGCGCTGTTTTAGt                                                                                                                        >  2:2724277/1‑150 (MQ=255)
                                 ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                                                                       <  1:95865/150‑1 (MQ=255)
                                 ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                                                                       <  2:910847/150‑1 (MQ=255)
                                       aTTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcatc                                                                                                                 <  2:4396585/150‑1 (MQ=255)
                                           cGTTAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGt                                                                                                             <  2:2360095/150‑1 (MQ=255)
                                             tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTcc                                                                                                           <  1:2949645/150‑1 (MQ=255)
                                             tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTcc                                                                                                           <  2:1994731/150‑1 (MQ=255)
                                                attttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTc                                                                                                        <  2:2217313/150‑1 (MQ=255)
                                                 ttttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCg                                                                                                       <  2:198290/150‑1 (MQ=255)
                                                    tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTc                                                                                                    <  2:639655/150‑1 (MQ=255)
                                                    tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTc                                                                                                    <  2:4238553/150‑1 (MQ=255)
                                                        attttatttATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTTTTTTGTTGACTCTCTTATATATATATTTATAATGGAAATAAATTAAAAGGTATTTATTATATTGCGCGATTATATTAGACATCATGTGCCTCGGTC‑TGAc                                                                                               >  2:3149383/1‑150 (MQ=255)
                                                         ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACg                                                                                              <  2:1578087/150‑1 (MQ=255)
                                                            tatttatAAACAAAATGCGTGTAGTGGCGTGTTGTTTTTTTATTTATTATGTTCACACTCTTATCTATATATTTTTAAGGCCAATAAATTAAAAGGAATTTATTATCATACGCTATTATAGTCGCCAACAAGTCCCCCGGTC‑GGACGGGt                                                                                           >  2:5184911/1‑150 (MQ=255)
                                                             atttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGtt                                                                                          <  1:4350089/150‑1 (MQ=255)
                                                                   aaaCAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTAAAACTATTATATATATTTTTATAAAGCAAATAAATTTATAGGAAATTATTATATTACGCGATTATATTAGCCAACATTTAACAAGGTC‑TTACGGGgtgttttg                                                                                    >  2:1611210/1‑146 (MQ=255)
                                                                   aaaCAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTCACGCTCGTATCTATATATTTATAAGGCAATTTAATGTAAAGGAATTTATTATAATACGCTATTATAGTCGCCCTCATGTCCCTCGGGA‑TGTCGGGTtgggttg                                                                                    >  2:2123257/1‑145 (MQ=255)
                                                                     aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTTTCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATATTCGCCATCATGTACCTCGGGC‑TGACGGGGTGTgatgat                                                                                  >  2:3102975/1‑150 (MQ=255)
                                                                     aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTAAAACACTTATATATAAATTTAAAAGGCAATTAAATGAAAAGGAATTTATTAAATTAAGCTATTATAATAGCCAACATGTCCCTCGGTC‑TGACGGGGGGTgttgat                                                                                  >  2:2687612/1‑150 (MQ=255)
                                                                      cAAAATGCGTGTAGTGGCGTTTTGTTTTTTTATTTATTATGTTAACTCTATTATCTATATATTTTTTAGGCAAATAAATGAAAAGGAATTTATTATATTGCGCGATTATAGTCGGCATCATGTCCCTCGGTC‑AGACGGGGTGtgttggtt                                                                                 >  2:3209596/1‑147 (MQ=255)
                                                                      cAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAATGAATTTATTATATTACGCTATTATAGTAGCCATCATTTCCCACGGTC‑TGACGGGTTGTGTTGATa                                                                                 >  2:4873535/1‑150 (MQ=255)
                                                                          aTGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTGACACTCATATATATATATTTTTAAGGCAAATAAATGTAAAGGAATTTATTATATTACCCGAATATATTCGCCAACATGTACCAAGGTCGTTAC‑GGTTGTGGTGATtaaaa                                                                             >  2:2498177/1‑150 (MQ=255)
                                                                          aTGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACACTCTTATCAATATATTTATAAGGCAAATAAATTTAAAGGAATTTATTATATTCCGCGATTATTGTCGCCAACATGTCCCGCGGTC‑TGTCGGGGTGGGTTGATaaaaa                                                                             >  2:2840086/1‑150 (MQ=255)
                                                                                 tAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGaa                                                                      <  1:1654341/150‑1 (MQ=255)
                                                                                   gTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAAc                                                                    <  1:2141215/150‑1 (MQ=255)
                                                                                      gCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGa                                                                 >  1:5136145/1‑150 (MQ=255)
                                                                                       cGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGaa                                                                >  2:1780773/1‑150 (MQ=255)
                                                                                        gTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCACTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGaaa                                                               >  2:1249517/1‑150 (MQ=255)
                                                                                         ttttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAAc                                                              <  1:3021265/150‑1 (MQ=255)
                                                                                           ttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGc                                                            >  1:2899140/1‑150 (MQ=255)
                                                                                                ttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCg                                                       >  1:1365468/1‑150 (MQ=255)
                                                                                                 tttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGc                                                      >  2:5037323/1‑150 (MQ=255)
                                                                                                  ttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGcc                                                     >  2:1085059/1‑150 (MQ=255)
                                                                                                   tCATTTATTATTTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCt                                                    >  2:1415268/1‑150 (MQ=255)
                                                                                                    cATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTg                                                   >  1:3383455/1‑150 (MQ=255)
                                                                                                     aTTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGc                                                  >  2:2148720/1‑150 (MQ=255)
                                                                                                       ttatttTGTTAACTCTATTATCTATATATTTATAAGGCTATTAAATGAAAAGGATTTTATTATCTTCCTATATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCa                                                <  1:4448313/150‑1 (MQ=255)
                                                                                                       ttattaTGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCa                                                <  1:3927939/150‑1 (MQ=255)
                                                                                                       ttattaTGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCa                                                >  1:3008394/1‑150 (MQ=255)
                                                                                                        tattatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCaa                                               >  1:3832799/1‑150 (MQ=255)
                                                                                                         attatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAAt                                              >  2:2418709/1‑150 (MQ=255)
                                                                                                           tatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATATTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAg                                            >  2:323582/1‑150 (MQ=255)
                                                                                                            atGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGt                                           >  1:2049741/1‑150 (MQ=255)
                                                                                                             tGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTc                                          <  2:5012868/150‑1 (MQ=255)
                                                                                                              gTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCa                                         >  1:4021943/1‑150 (MQ=255)
                                                                                                              gTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCa                                         <  1:211339/150‑1 (MQ=255)
                                                                                                               ttCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCaa                                        <  1:2492150/150‑1 (MQ=255)
                                                                                                                tCACTCTCTTATCTATATATTTATAAGGCAATAAAATGAAAAGGAATTTATTATCTTCCGCTATAATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTCCCAAAAGTCAAc                                       >  1:4334684/1‑150 (MQ=255)
                                                                                                                  aCTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCt                                     >  2:4054508/1‑150 (MQ=255)
                                                                                                                    tctctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGc                                   >  2:4030985/1‑150 (MQ=255)
                                                                                                                          aTCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCAcc                             >  1:3463437/1‑150 (MQ=255)
                                                                                                                             tatataTTTATAAGGAAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGc                          <  1:3419520/150‑1 (MQ=255)
                                                                                                                               tatatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTc                        <  2:1733509/150‑1 (MQ=255)
                                                                                                                                atatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTcc                       >  2:3387685/1‑150 (MQ=255)
                                                                                                                                atatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTcc                       >  1:4584046/1‑150 (MQ=255)
                                                                                                                                atatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTcc                       >  2:2871647/1‑150 (MQ=255)
                                                                                                                                   tttATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGa                    <  2:3894975/150‑1 (MQ=255)
                                                                                                                                    ttATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTTCCAATAGTCAACCTGCCGCACCAGCTCCTGaa                   <  2:3849948/150‑1 (MQ=255)
                                                                                                                                       taAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCg                >  1:1143288/1‑150 (MQ=255)
                                                                                                                                        aaGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGa               >  2:5222573/1‑150 (MQ=255)
                                                                                                                                          ggCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGaaa             >  1:223061/1‑150 (MQ=255)
                                                                                                                                          ggCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGaaa             <  2:3445213/150‑1 (MQ=255)
                                                                                                                                          ggCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTCCCAATAGTCAACCTGCCGCACCAGCACCTGAAGCGaaa             >  2:4783947/1‑150 (MQ=255)
                                                                                                                                           gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc            >  1:805798/1‑150 (MQ=255)
                                                                                                                                            cAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGACGCGAAAcc           >  1:2016152/1‑150 (MQ=255)
                                                                                                                                              attaaattaaAAGGAATTTATTATCTTCCGATATTATAGTCGCAATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTa         <  1:117879/150‑1 (MQ=255)
                                                                                                                                              aTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTa         >  1:3174742/1‑150 (MQ=255)
                                                                                                                                              aTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTa         >  2:512248/1‑150 (MQ=255)
                                                                                                                                              aTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTa         <  2:3996754/150‑1 (MQ=255)
                                                                                                                                                tAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACt       >  1:1720895/1‑150 (MQ=255)
                                                                                                                                                 aaaTGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTg      >  1:3740754/1‑150 (MQ=255)
                                                                                                                                                    tGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAGGCGAAACCTACTGAAg   >  2:4661161/1‑150 (MQ=255)
                                                                                                                                                     gAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGc  >  1:4657807/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTC‑TGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGC  >  CP009273/4080215‑4080513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑