| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,214,563 | A→T | 100% | T270S (ACC→TCC) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,214,563 | 0 | A | T | 99.6% | 898.2 / ‑4.7 | 269 | T270S (ACC→TCC) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (120/148); total (120/149) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 269 total aligned reads displayed.
CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATT > CP009273/4214416‑4214709 | cgcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc < 1:2261686/150‑1 (MQ=255)cgcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc < 1:730243/150‑1 (MQ=255)cgcACGCTCTCCGGGCAGACCAACGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTTGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTcc < 2:3540817/150‑1 (MQ=255) gcACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGACACGGATTGCGGAATGCTACGTCTCcg > 1:602293/1‑150 (MQ=255) cACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCcgc > 1:1200253/1‑150 (MQ=255) ctcCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCAccc > 2:5044626/1‑150 (MQ=255) gggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAc < 2:304353/150‑1 (MQ=255) gggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAAc > 1:2844660/1‑150 (MQ=255) ggCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACg < 1:3994281/150‑1 (MQ=255) gCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGc < 2:3894183/150‑1 (MQ=255) cAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGcc < 2:2993169/150‑1 (MQ=255) aGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCg < 2:2352764/150‑1 (MQ=255) caccGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCt < 2:2982287/150‑1 (MQ=255) cGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTAcc > 2:264531/1‑150 (MQ=255) cGAAGCATTTTACAACTCAGTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACGGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGACCGCCGGGCTAcc < 2:459695/150‑1 (MQ=255) aggCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCa > 1:2666180/3‑150 (MQ=255) aGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCaa > 1:3819978/1‑150 (MQ=255) aGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCaa > 1:3484998/1‑150 (MQ=255) ttttACAACTCNTTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGcc > 1:2762649/1‑150 (MQ=255) aCTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTg < 2:2881727/150‑1 (MQ=255) cTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGa > 1:4762380/1‑150 (MQ=255) ttGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTAc < 2:2271282/150‑1 (MQ=255) gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc > 2:4814071/1‑150 (MQ=255) gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc < 1:5178677/150‑1 (MQ=255) gcCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtc > 2:3935955/1‑150 (MQ=255) ccACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtct < 1:3316325/150‑1 (MQ=255) cACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtctc < 2:4718593/150‑1 (MQ=255) aCGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGAtctca < 2:4979324/150‑2 (MQ=255) gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAc < 2:3681181/150‑1 (MQ=255) gCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGAc < 2:2507434/150‑1 (MQ=255) ccGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACACCGGGCTACCCAACGCCTTTGGTGAGTCCGATCTCGACg < 1:4904560/150‑1 (MQ=255) cGAAGCTCTGACCTTTGGACTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAAAGCTACGTCTCAGCGCACCCGAACGCCGGGCTACACAACGCCTTTGGTGAGTACGATCTCGACGc > 2:4911627/1‑150 (MQ=255) aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGACTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg > 2:1061043/1‑150 (MQ=255) aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg > 1:2459958/1‑150 (MQ=255) aaGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCg > 1:4816784/1‑150 (MQ=255) aGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGa > 1:3055353/1‑150 (MQ=255) ctctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGaca < 2:2199055/150‑1 (MQ=255) ctctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGaca < 1:2613362/150‑1 (MQ=255) tGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGa > 2:1505252/1‑150 (MQ=255) gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt < 2:4323845/150‑1 (MQ=255) gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt < 2:2110056/150‑1 (MQ=255) gACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGAt < 2:3057678/150‑1 (MQ=255) cTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGc < 2:5329722/150‑1 (MQ=255) tGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaa > 2:5104893/1‑150 (MQ=255) tGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCaaa > 2:1987429/1‑150 (MQ=255) tGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa < 2:625609/150‑1 (MQ=255) tGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGa > 1:357141/1‑150 (MQ=255) gAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGAt > 2:3844688/1‑150 (MQ=255) aaCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATa > 1:2257770/1‑150 (MQ=255) tgtgCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGt < 1:4164175/150‑1 (MQ=255) cgcTGGGGCCCGATGAACTGCGACAGTTCGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAAt < 2:1115136/150‑1 (MQ=255) gcTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCTGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTTCGATATCGACGCCGACACGATGGCAAAACAGATACGTGATTg < 1:2116890/150‑1 (MQ=255) cTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATgg < 1:3029033/150‑1 (MQ=255) ggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGgcgc < 2:4627644/150‑1 (MQ=255) ggCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGgcgc < 1:5267002/150‑1 (MQ=255) cGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc < 1:4552836/150‑1 (MQ=255) cGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGc > 2:1774936/1‑150 (MQ=255) aTGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCgg < 1:3222344/150‑1 (MQ=255) aTGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCgg < 1:2695279/150‑1 (MQ=255) gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt > 2:4115043/1‑150 (MQ=255) gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt < 1:1454260/150‑1 (MQ=255) gAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGt > 1:135329/1‑150 (MQ=255) aCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGttt < 2:3371680/150‑1 (MQ=255) gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct < 2:166358/150‑1 (MQ=255) gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct < 1:4030963/150‑1 (MQ=255) gcgcCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTtct < 1:2005872/150‑1 (MQ=255) cAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAat < 2:3954939/150‑1 (MQ=255) cAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAat < 2:4442640/150‑1 (MQ=255) gTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAatat < 1:605996/150‑1 (MQ=255) tACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtc < 1:1291768/150‑1 (MQ=255) tACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtc < 1:2125259/150‑1 (MQ=255) gTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgtc > 1:989549/1‑150 (MQ=255) gTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAtcgtc > 2:236121/1‑150 (MQ=255) gCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcgg < 1:1730388/150‑1 (MQ=255) gCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcgg < 1:1916658/150‑1 (MQ=255) cAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggc < 1:2055271/150‑1 (MQ=255) aGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcg > 1:2801558/1‑150 (MQ=255) aGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcg > 1:4243914/1‑150 (MQ=255) ggAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTcggcgg > 2:4128224/1‑150 (MQ=255) gCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGgctg > 1:487237/1‑150 (MQ=255) gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg > 2:5155997/1‑150 (MQ=255) gTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGctgctg > 1:3910861/1‑150 (MQ=255) tCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCtgt < 1:2183481/150‑1 (MQ=255) cGGATTGAGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGCTACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGc < 1:4638778/150‑1 (MQ=255) ggATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGca < 2:3672621/150‑1 (MQ=255) ggATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGca > 1:2949608/1‑150 (MQ=255) ggAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGcc > 2:3276976/1‑150 (MQ=255) gAATGCTACGTCTCCGCGCACCCGACCGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATCCGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca < 1:813016/150‑1 (MQ=255) gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca > 1:4998776/1‑150 (MQ=255) gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCca > 1:5090802/1‑150 (MQ=255) gAATGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCATTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCTTCGGCGGATGCTGTGTCACCACGCca > 1:4399169/1‑150 (MQ=255) aaTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcac > 2:618626/1‑150 (MQ=255) aTGCTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCcaca < 2:117496/150‑1 (MQ=255) cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCACTATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca < 2:4240453/150‑1 (MQ=255) cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca > 2:4740244/1‑150 (MQ=255) cTACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaaca > 2:3171824/1‑150 (MQ=255) tACGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCGTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCacaacag > 2:5244034/1‑149 (MQ=255) aCGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACata > 1:1636442/1‑150 (MQ=255) cGTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACatat > 2:767533/1‑150 (MQ=255) gTCTCCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATAtt > 2:3076335/1‑150 (MQ=255) | CGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATT > CP009273/4214416‑4214709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |