Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,219,858 G→T 61.3% V71V (GTC→GTA)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,219,8580GT61.3% 131.7 / inf 313V71V (GTC→GTA) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (61/60);  new base T (95/97);  total (156/157)
Fisher's exact test for biased strand distribution p-value = 9.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

GCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAG  >  CP009273/4219709‑4220004
                                                                                                                                                     |                                                                                                                                                  
  gcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGAc                                                                                                                                                  <  1:3570119/150‑1 (MQ=255)
  gcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGAc                                                                                                                                                  >  2:3451627/1‑150 (MQ=255)
     ccAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAAc                                                                                                                                               <  2:157105/150‑1 (MQ=255)
       aGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCACCGGGATCTACAACGc                                                                                                                                             >  2:3520759/1‑150 (MQ=255)
       aGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGc                                                                                                                                             >  2:277887/1‑150 (MQ=255)
          cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTAt                                                                                                                                          <  2:4617520/150‑1 (MQ=255)
               taCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATa                                                                                                                                     <  2:3060401/150‑1 (MQ=255)
                      cgcCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGa                                                                                                                              >  2:4512978/1‑150 (MQ=255)
                        ccACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAGTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACa                                                                                                                            <  2:2121829/150‑1 (MQ=255)
                             tttAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAac                                                                                                                       <  1:2087841/150‑1 (MQ=255)
                               tAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAacac                                                                                                                     >  1:3996015/1‑150 (MQ=255)
                                aaCCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAcc                                                                                                                    >  1:1387470/1‑150 (MQ=255)
                                  ccACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCa                                                                                                                  <  2:1450820/150‑1 (MQ=255)
                                  ccACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCa                                                                                                                  <  1:2989696/150‑1 (MQ=255)
                                      gTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCgg                                                                                                              >  2:928724/1‑150 (MQ=255)
                                         aGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGc                                                                                                           <  1:3330194/150‑1 (MQ=255)
                                          gTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCg                                                                                                          >  2:2306614/1‑150 (MQ=255)
                                           tAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCga                                                                                                         <  1:3079812/150‑1 (MQ=255)
                                               tGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGGAACACCCAGCGGAGCGAGAAc                                                                                                     <  2:1856877/150‑1 (MQ=255)
                                                ggTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTAGTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAAcc                                                                                                    <  2:1105964/150‑1 (MQ=255)
                                                      agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                                                                                              >  1:4994536/1‑150 (MQ=255)
                                                      agcagcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                                                                                              >  2:64403/1‑150 (MQ=255)
                                                      agcagcCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGcc                                                                                              >  2:556963/1‑150 (MQ=255)
                                                         agcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                                                                                           >  2:1952186/1‑150 (MQ=255)
                                                         agcCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                                                                                           >  1:1716787/1‑150 (MQ=255)
                                                          gcCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGttt                                                                                          <  2:146849/150‑1 (MQ=255)
                                                           cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                                                                                         <  2:3582299/150‑1 (MQ=255)
                                                            ccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTc                                                                                        <  1:2239218/150‑1 (MQ=255)
                                                                  tCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCtgtg                                                                                  <  2:4854939/150‑1 (MQ=255)
                                                                   cgcgGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTgtgt                                                                                 <  1:800410/150‑1 (MQ=255)
                                                                    gcgGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTa                                                                                >  1:2799905/1‑150 (MQ=255)
                                                                      ggCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAat                                                                              >  2:4569204/1‑150 (MQ=255)
                                                                       gCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc                                                                             <  1:4626393/150‑1 (MQ=255)
                                                                         aCTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTTatcat                                                                           <  2:1032282/150‑1 (MQ=255)
                                                                          cTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc                                                                          >  2:2012609/1‑150 (MQ=255)
                                                                           tGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc                                                                         <  2:4048991/150‑1 (MQ=255)
                                                                           tGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc                                                                         <  2:4269202/150‑1 (MQ=255)
                                                                            gTTTCTGCAACTGGACAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATccc                                                                        <  2:1703713/150‑1 (MQ=255)
                                                                            gTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATccc                                                                        >  1:3500779/1‑150 (MQ=255)
                                                                            gTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATccc                                                                        <  1:2386021/150‑1 (MQ=255)
                                                                             tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCa                                                                       <  2:3791833/150‑1 (MQ=255)
                                                                              ttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAg                                                                      <  2:5018994/150‑1 (MQ=255)
                                                                               tCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAgg                                                                     <  1:4402734/150‑1 (MQ=255)
                                                                                  gCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCt                                                                  <  2:4118481/150‑1 (MQ=255)
                                                                                   cAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTg                                                                 <  2:1206662/150‑1 (MQ=255)
                                                                                       tGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGtt                                                             <  2:2184591/150‑1 (MQ=255)
                                                                                       tGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGtt                                                             <  2:2598928/150‑1 (MQ=255)
                                                                                         gAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTAc                                                           >  1:4202624/1‑150 (MQ=255)
                                                                                         gAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTAc                                                           >  1:5015363/1‑150 (MQ=255)
                                                                                          aaaGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACg                                                          <  2:1278626/150‑1 (MQ=255)
                                                                                          aaaGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACg                                                          >  1:285998/1‑150 (MQ=255)
                                                                                           aaGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGc                                                         >  1:3706547/1‑150 (MQ=255)
                                                                                            aGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGcc                                                        >  1:3891706/1‑150 (MQ=255)
                                                                                               aTTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGc                                                     >  1:4838829/1‑150 (MQ=255)
                                                                                                ttCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCg                                                    >  2:3928903/1‑150 (MQ=255)
                                                                                                 tCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGa                                                   <  1:3025044/150‑1 (MQ=255)
                                                                                                   cccccgccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGaaa                                                 >  1:730893/4‑150 (MQ=255)
                                                                                                     gccgccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAgg                                               <  1:1000381/150‑1 (MQ=255)
                                                                                                        gccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGAt                                            <  1:311996/150‑1 (MQ=255)
                                                                                                        gccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGAt                                            >  1:1177481/1‑150 (MQ=255)
                                                                                                         ccgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATa                                           <  1:4780316/150‑1 (MQ=255)
                                                                                                          cgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaa                                          <  2:2566574/150‑1 (MQ=255)
                                                                                                          cgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaa                                          >  1:104426/1‑150 (MQ=255)
                                                                                                          cgACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaa                                          >  1:3381606/1‑150 (MQ=255)
                                                                                                           gACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATaaa                                         >  1:3449560/1‑150 (MQ=255)
                                                                                                            aCAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAac                                        <  1:9863/150‑1 (MQ=255)
                                                                                                             cAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAaca                                       <  2:2239801/150‑1 (MQ=255)
                                                                                                              aaTCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAacac                                      >  1:1437291/1‑150 (MQ=255)
                                                                                                                 cACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGc                                   >  2:4314161/1‑150 (MQ=255)
                                                                                                                 cACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGc                                   >  2:468794/1‑150 (MQ=255)
                                                                                                                 cACGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGc                                   >  2:1592919/1‑150 (MQ=255)
                                                                                                                  aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCt                                  <  2:4759687/150‑1 (MQ=255)
                                                                                                                  aCGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCt                                  >  1:1960437/1‑150 (MQ=255)
                                                                                                                   cGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAAAACCGCTg                                 >  1:4132123/1‑150 (MQ=255)
                                                                                                                    gATCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGa                                >  1:1266571/1‑150 (MQ=255)
                                                                                                                     aTCTCAGCATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGTTCTGCGTTAAGCCAGCGAAAGGGATAAACACCGCTGAg                               <  2:3037127/150‑1 (MQ=255)
                                                                                                                     aTCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAg                               >  2:1268/1‑150 (MQ=255)
                                                                                                                     aTCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAAAACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGAATTCGCTGAGTAATCATCCCAGGTCTGCGTTACGCCAGCGAACGGGATAAACACCGCTGAg                               >  2:3315154/1‑150 (MQ=255)
                                                                                                                       ctcAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcg                             <  1:380377/150‑1 (MQ=255)
                                                                                                                        tcAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcgg                            >  2:3364818/1‑150 (MQ=255)
                                                                                                                         cAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcggc                           >  1:2843295/1‑150 (MQ=255)
                                                                                                                          aGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAgcggcg                          <  1:1593651/150‑1 (MQ=255)
                                                                                                                            cATTTTCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGAc                        >  2:792814/1‑150 (MQ=255)
                                                                                                                              ttttCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGAccc                      <  2:2875478/150‑1 (MQ=255)
                                                                                                                                ttCAATCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTg                    <  1:1846298/150‑1 (MQ=255)
                                                                                                                                 tCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGc                   <  2:3400400/150‑1 (MQ=255)
                                                                                                                                   aaTCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCaa                 >  2:440546/1‑150 (MQ=255)
                                                                                                                                   aaTCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTGTTCGCAGTGTAATCAACCCACGTCTGCGTTACGCCCGCGAAAGGGAAAAACACCGCTGAGCGGCGACCCGGCaa                 >  1:3750213/1‑150 (MQ=255)
                                                                                                                                    aTCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAAc                >  2:2464643/1‑150 (MQ=255)
                                                                                                                                     tCGCGGCAACGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACt               <  1:4307531/150‑1 (MQ=255)
                                                                                                                                      cgcgGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTg              >  2:2107482/1‑150 (MQ=255)
                                                                                                                                            aaCGGGATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGc        >  2:5247320/1‑150 (MQ=255)
                                                                                                                                            aaCGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGc        <  2:3661535/150‑1 (MQ=255)
                                                                                                                                               gggATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGATACGCCAGCGAAAGGGATAAACACCGCTGAGAGGCGACCCAGCAACTGTTCATCAAt     >  1:5273232/1‑150 (MQ=255)
                                                                                                                                               gggATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAAt     >  1:2485393/1‑150 (MQ=255)
                                                                                                                                               gggATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTATGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAAt     >  1:4529765/1‑150 (MQ=255)
                                                                                                                                                ggATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAAtt    >  1:843468/1‑150 (MQ=255)
                                                                                                                                                ggATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAAtt    <  2:1826476/150‑1 (MQ=255)
                                                                                                                                                 gATCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATAATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTa   >  1:57230/1‑150 (MQ=255)
                                                                                                                                                  aTCTACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAg  >  1:2201280/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCAGGTCTGCGTTACGCCAGCGAAAGGGATAAACACCGCTGAGCGGCGACCCTGCAACTGTTCAGCAATTAG  >  CP009273/4219709‑4220004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑