Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,255,009 A→T 100% V256V (GTA→GTT)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,255,0090AT99.2% 1155.7 / ‑1.5 349V256V (GTA→GTT) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base T (179/167);  total (181/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 349 total aligned reads displayed.

AAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGCT  >  CP009273/4254862‑4255155
                                                                                                                                                   |                                                                                                                                                  
aaaCACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCt                                                                                                                                                  >  2:4214856/1‑150 (MQ=255)
 aaCACCGTTTATGACTATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCtt                                                                                                                                                 <  1:2671309/150‑1 (MQ=255)
 aaCACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCtt                                                                                                                                                 <  2:1351757/150‑1 (MQ=255)
 aaCACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCtt                                                                                                                                                 <  2:4312157/150‑1 (MQ=255)
  acacCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTa                                                                                                                                                <  2:4356414/150‑1 (MQ=255)
    acCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtc                                                                                                                                              <  1:3438821/150‑1 (MQ=255)
    acCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtc                                                                                                                                              <  2:175500/150‑1 (MQ=255)
    acCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtc                                                                                                                                              >  2:1477518/1‑150 (MQ=255)
     ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtct                                                                                                                                             <  2:2339760/150‑1 (MQ=255)
     ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtct                                                                                                                                             >  2:1316937/1‑150 (MQ=255)
       ggTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCTCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                           <  1:2739017/150‑1 (MQ=255)
        gTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                          >  1:3422941/1‑150 (MQ=255)
         ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTtctc                                                                                                                                         >  1:502391/1‑150 (MQ=255)
           aTGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTATTATCTTCTCGc                                                                                                                                       >  2:718813/1‑150 (MQ=255)
             gACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTg                                                                                                                                     >  1:3669823/1‑150 (MQ=255)
             gACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGACGCAGCGCGTCAGTCGATGGGTAAAACAACATTTGCGATGAACCTCGACGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTAAACTTCTCTCTg                                                                                                                                     >  1:571752/1‑150 (MQ=255)
               cGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGga                                                                                                                                   <  2:5241864/150‑1 (MQ=255)
                gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgag                                                                                                                                  >  2:1140593/1‑150 (MQ=255)
                gATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGgag                                                                                                                                  >  2:1979704/1‑150 (MQ=255)
                 aTCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgaga                                                                                                                                 >  2:4537448/1‑150 (MQ=255)
                  tctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGAt                                                                                                                                <  2:2081862/150‑1 (MQ=255)
                  tctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGAt                                                                                                                                <  2:2635466/150‑1 (MQ=255)
                   ctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATg                                                                                                                               <  1:4718293/150‑1 (MQ=255)
                      aacaaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCa                                                                                                                            <  2:3430587/150‑1 (MQ=255)
                       acaaAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCAt                                                                                                                           <  2:4475555/150‑1 (MQ=255)
                         aaaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCa                                                                                                                         <  2:621424/150‑1 (MQ=255)
                          aaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAg                                                                                                                        <  2:5134888/150‑1 (MQ=255)
                             aaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAAc                                                                                                                     <  1:895503/150‑1 (MQ=255)
                             aaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAAc                                                                                                                     <  2:2865644/150‑1 (MQ=255)
                              aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACa                                                                                                                    <  2:4856807/150‑1 (MQ=255)
                               aCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAg                                                                                                                   <  2:596310/150‑1 (MQ=255)
                                  gCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATc                                                                                                                >  2:306810/1‑150 (MQ=255)
                                    tGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCATAACAGATCat                                                                                                              <  1:1464778/150‑1 (MQ=255)
                                    tGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCat                                                                                                              >  2:4053217/1‑150 (MQ=255)
                                       cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgat                                                                                                           <  1:3600637/150‑1 (MQ=255)
                                       cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgat                                                                                                           >  1:2988710/1‑150 (MQ=255)
                                         tGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGc                                                                                                         >  1:3909359/1‑150 (MQ=255)
                                         tGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGc                                                                                                         <  2:877172/150‑1 (MQ=255)
                                         tGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGc                                                                                                         <  2:1073628/150‑1 (MQ=255)
                                          gCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCg                                                                                                        >  1:1827570/1‑150 (MQ=255)
                                           cAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGt                                                                                                       >  2:3261660/1‑150 (MQ=255)
                                               cGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                   >  2:1505616/1‑150 (MQ=255)
                                               cGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                   >  1:4971927/1‑150 (MQ=255)
                                                 tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTg                                                                                                 >  1:490313/1‑150 (MQ=255)
                                                 tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTg                                                                                                 <  1:3507318/150‑1 (MQ=255)
                                                 tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTg                                                                                                 <  1:4453732/150‑1 (MQ=255)
                                                   ggATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                               >  1:3926635/1‑150 (MQ=255)
                                                    gATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCg                                                                                              >  1:1725706/1‑150 (MQ=255)
                                                     aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGt                                                                                             >  2:3167438/1‑150 (MQ=255)
                                                       ttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCg                                                                                           >  1:969371/1‑150 (MQ=255)
                                                       ttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCg                                                                                           >  1:2340012/1‑150 (MQ=255)
                                                         gATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCt                                                                                         >  1:2218103/1‑150 (MQ=255)
                                                         gATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCt                                                                                         >  2:3027860/1‑150 (MQ=255)
                                                              tcgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgc                                                                                    >  2:1613113/1‑150 (MQ=255)
                                                                    ccgcGCGTCCGTCGATGTGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGTTCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTg                                                                              <  1:482465/150‑1 (MQ=255)
                                                                           tccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGAc                                                                       <  2:3528934/150‑1 (MQ=255)
                                                                                cGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTaaaa                                                                  <  1:1171304/150‑1 (MQ=255)
                                                                                 gATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAAt                                                                 >  2:4607631/1‑150 (MQ=255)
                                                                                  aTGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATc                                                                <  2:275236/150‑1 (MQ=255)
                                                                                     ggTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGt                                                             >  1:1656799/1‑150 (MQ=255)
                                                                                        aaaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAcc                                                          <  1:2084627/150‑1 (MQ=255)
                                                                                         aaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCg                                                         >  2:4635418/1‑150 (MQ=255)
                                                                                         aaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCg                                                         >  1:689690/1‑150 (MQ=255)
                                                                                            caacaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGc                                                      <  1:2400161/150‑1 (MQ=255)
                                                                                              acaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGTAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAg                                                    >  2:3751988/1‑150 (MQ=255)
                                                                                              acaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAg                                                    <  2:4107781/150‑1 (MQ=255)
                                                                                                aTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCt                                                  >  2:4336356/1‑150 (MQ=255)
                                                                                                aTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCt                                                  >  1:3160254/1‑150 (MQ=255)
                                                                                                aTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCt                                                  >  2:4757818/1‑150 (MQ=255)
                                                                                                aTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCt                                                  <  1:3233968/150‑1 (MQ=255)
                                                                                                  ttGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCg                                                <  1:1081849/150‑1 (MQ=255)
                                                                                                        tGAACCTCGTAGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACg                                          <  1:5274361/150‑1 (MQ=255)
                                                                                                         gAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGa                                         >  1:4674556/1‑150 (MQ=255)
                                                                                                         gAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGa                                         >  2:187629/1‑150 (MQ=255)
                                                                                                         gAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGa                                         >  1:4154157/1‑150 (MQ=255)
                                                                                                          aaCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGaa                                        >  2:2099719/1‑150 (MQ=255)
                                                                                                          aaCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGaa                                        >  2:3879020/1‑150 (MQ=255)
                                                                                                            ccTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                      <  2:4259383/150‑1 (MQ=255)
                                                                                                            ccTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGa                                      <  2:1730078/150‑1 (MQ=255)
                                                                                                             cTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGAc                                     >  1:2883722/1‑150 (MQ=255)
                                                                                                                gtcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTgg                                  >  2:4955378/1‑150 (MQ=255)
                                                                                                                  cgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgc                                <  1:955559/150‑1 (MQ=255)
                                                                                                                   gAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcg                               >  1:139407/1‑150 (MQ=255)
                                                                                                                   gAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcg                               >  2:4098962/1‑150 (MQ=255)
                                                                                                                         gcggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtt                         <  1:1186621/150‑1 (MQ=255)
                                                                                                                         gcggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtt                         >  1:2190771/1‑150 (MQ=255)
                                                                                                                           ggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGACGCTGTCGAGCGTTGACCAGACTAAAATCCGTAACGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTc                       >  2:5134077/1‑150 (MQ=255)
                                                                                                                            gcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGTCAGCTCGATGACGAAGACTGGGCGCGAATTTcc                      <  2:4813004/150‑1 (MQ=255)
                                                                                                                            gcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAtttac                      <  2:2216826/150‑3 (MQ=255)
                                                                                                                               aTGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGc                   >  2:4906217/1‑150 (MQ=255)
                                                                                                                                  ttGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCAcc                <  2:2619740/150‑1 (MQ=255)
                                                                                                                                   tGCAGGATCAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCAGGCACCa               >  1:3726792/1‑150 (MQ=255)
                                                                                                                                       ggATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATggg           >  1:453355/1‑150 (MQ=255)
                                                                                                                                         aTAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTa         <  2:5304151/150‑1 (MQ=255)
                                                                                                                                         aTAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGATCTCTGGCGTCGCTGTCGCGCGTTGAACAGACTAAAATCCGTACCGGGCAGCACGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgggac         >  2:2425477/1‑148 (MQ=255)
                                                                                                                                           aaaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt       >  1:794631/1‑150 (MQ=255)
                                                                                                                                            aaCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt      >  1:525798/1‑150 (MQ=255)
                                                                                                                                             aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtttt     <  2:4135309/150‑1 (MQ=255)
                                                                                                                                             aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtttt     <  2:2160963/150‑1 (MQ=255)
                                                                                                                                                ggTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGCt  <  1:4107563/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTGCT  >  CP009273/4254862‑4255155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑