BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,274,337	T→A	6.8%	Q165L (CAG→CTG)	actP ←	acetate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,274,337	0	T	A	6.8%	780.6 / 34.6	278	Q165L (CAG→CTG)	actP	acetate transporter
Reads supporting (aligned to +/- strand): ref base T (114/141); new base A (8/11); total (122/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 283 total aligned reads displayed.

ACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATC  >  CP009273/4274188‑4274486

 cagcaCGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTg                                                                                                                                                      >  1:2212359/1‑150 (MQ=255)

 cagcaCGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTg                                                                                                                                                      >  2:1764160/1‑150 (MQ=255)

   gcaCGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTggg                                                                                                                                                    >  1:4184106/1‑150 (MQ=255)

         cTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATaa                                                                                                                                              >  2:5220614/1‑150 (MQ=255)

          tttGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAg                                                                                                                                             <  2:1983857/150‑1 (MQ=255)

          tttGATAATTTGCACCCAGGTGGTCGACAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAg                                                                                                                                             <  1:2367345/150‑1 (MQ=255)

           ttGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAgg                                                                                                                                            <  1:1306148/150‑1 (MQ=255)

             gATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGTTa                                                                                                                                          >  1:1007144/1‑150 (MQ=255)

              aTAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTaa                                                                                                                                         >  1:933709/1‑150 (MQ=255)

                aaTTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAg                                                                                                                                       <  1:1977282/150‑1 (MQ=255)

                 aTTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAgc                                                                                                                                      >  1:2851599/1‑150 (MQ=255)

                  tttGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAgcg                                                                                                                                     <  2:4180541/150‑1 (MQ=255)

                       aCCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGccacc                                                                                                                                <  1:3016401/150‑1 (MQ=255)

                              tggtCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGccaccaccacca                                                                                                                         <  1:527666/150‑1 (MQ=255)

                                 tCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCagag                                                                                                                      <  1:2983827/150‑1 (MQ=255)

                                    ccAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCTCAATGTGATAGTTAAGGCCAAACAGCAGCTCGAACAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAAc                                                                                                                   <  1:4510911/150‑1 (MQ=255)

                                      aGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACca                                                                                                                 <  2:2357134/150‑1 (MQ=255)

                                         aTGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAg                                                                                                              <  1:4664902/150‑1 (MQ=255)

                                          tGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCGCCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAgg                                                                                                             <  2:311076/150‑1 (MQ=255)

                                          tGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAgg                                                                                                             <  2:579139/150‑1 (MQ=255)

                                          tGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAgg                                                                                                             >  1:1172654/1‑150 (MQ=255)

                                                    cAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAAt                                                                                                   <  1:1909900/150‑1 (MQ=255)

                                                    cAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAAt                                                                                                   >  1:864223/1‑150 (MQ=255)

                                                     aGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATa                                                                                                  >  2:2870348/1‑150 (MQ=255)

                                                      ggACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATAc                                                                                                 <  1:3249842/150‑1 (MQ=255)

                                                       gACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACg                                                                                                <  1:4277713/150‑1 (MQ=255)

                                                       gACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACg                                                                                                <  1:3846470/150‑1 (MQ=255)

                                                         cGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGaa                                                                                              <  2:4703916/150‑1 (MQ=255)

                                                           tACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATc                                                                                            <  1:1166703/150‑1 (MQ=255)

                                                            aCATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCg                                                                                           <  1:1364675/150‑1 (MQ=255)

                                                               tcatcatcaGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGca                                                                                        <  1:1062591/150‑2 (MQ=255)

                                                                  tcatcaGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCtt                                                                                     <  2:832920/150‑1 (MQ=255)

                                                                      caGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGttt                                                                                 <  2:718831/150‑1 (MQ=255)

                                                                       aGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTc                                                                                <  1:4388098/150‑1 (MQ=255)

                                                                       aGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTc                                                                                >  1:4886351/1‑150 (MQ=255)

                                                                       aGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTc                                                                                >  2:2585623/1‑150 (MQ=255)

                                                                           cGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGAc                                                                            >  1:533019/1‑150 (MQ=255)

                                                                           cGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGAc                                                                            <  1:1518242/150‑1 (MQ=255)

                                                                           cGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGAc                                                                            >  1:1883997/1‑150 (MQ=255)

                                                                              cGACCAGCACCCCCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATACGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAAGCGGCCCTTGTTTCAGACGGt                                                                         <  2:2470183/150‑1 (MQ=255)

                                                                               gACCAGCACCACCGCAATTTGATAGTTAAGGCAAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATAAGGGAGATAAGTTAAAGCGCCACCACCACCATAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGTCGGTa                                                                        <  2:349546/150‑1 (MQ=255)

                                                                                aCCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTaa                                                                       >  1:4547868/1‑150 (MQ=255)

                                                                                 ccAGCACCACCGAAATGTTATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCCCCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAag                                                                      <  1:1416372/150‑1 (MQ=255)

                                                                                  cAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGACTCGGCCCTTGTTTCAGACGGTAaga                                                                     <  1:3924860/150‑1 (MQ=255)

                                                                                   aGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAagag                                                                    >  2:2074129/1‑150 (MQ=255)

                                                                                   aGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAagag                                                                    >  1:3689630/1‑150 (MQ=255)

                                                                                      accaccGCACTGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTTAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTCAGAGGcc                                                                 >  2:602203/1‑150 (MQ=255)

                                                                                      accaccGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCTGCCCGTGTTTCAGACGGTAAGAGGcc                                                                 >  1:227480/1‑150 (MQ=255)

                                                                                      accaccGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGcc                                                                 <  2:1027414/150‑1 (MQ=255)

                                                                                         accGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCcaca                                                              >  2:3235571/1‑150 (MQ=255)

                                                                                         accGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCcaca                                                              >  2:4922592/1‑150 (MQ=255)

                                                                                            gCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCg                                                           <  2:1151427/150‑1 (MQ=255)

                                                                                             cAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCgg                                                          >  2:4235272/1‑150 (MQ=255)

                                                                                                tgtgATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCaa                                                       >  2:538943/1‑150 (MQ=255)

                                                                                                 gtgATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCaaa                                                      <  1:4311946/150‑1 (MQ=255)

                                                                                                  tgATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAg                                                     >  2:1057777/1‑150 (MQ=255)

                                                                                                  tgATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAg                                                     >  1:1640824/1‑150 (MQ=255)

                                                                                                   gATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAgg                                                    >  1:4039392/1‑150 (MQ=255)

                                                                                                     tAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGgtg                                                  >  2:4872097/1‑150 (MQ=255)

                                                                                                     tAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGgtg                                                  >  1:3800238/1‑150 (MQ=255)

                                                                                                        ttAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAg                                               <  1:2797301/150‑1 (MQ=255)

                                                                                                          aaGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGTGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCTGCAAAGGTGTAgcg                                             >  1:2813061/1‑150 (MQ=255)

                                                                                                          aaGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAgcg                                             >  1:224256/1‑150 (MQ=255)

                                                                                                          aaGGCCAAAAAGAAGCTCTATAAGTTTACCTGCGCACACCATCTGGGAGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCAGAAAGAATACGAATCGTCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAgcg                                             <  1:3206267/150‑1 (MQ=255)

                                                                                                           aGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAgcgc                                            >  1:1511725/1‑150 (MQ=255)

                                                                                                            ggCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGcc                                           <  2:2668334/150‑1 (MQ=255)

                                                                                                              ccAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGcccc                                         >  1:3227312/1‑150 (MQ=255)

                                                                                                               aaaaCAGCAGCTAGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCa                                        <  2:3028346/149‑1 (MQ=255)

                                                                                                                 aaCAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAgg                                      >  2:4245137/1‑150 (MQ=255)

                                                                                                                  aCAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGt                                     >  1:1759342/1‑150 (MQ=255)

                                                                                                                    agcagcTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTa                                   >  2:2639014/1‑150 (MQ=255)

                                                                                                                       agcTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGc                                >  1:258345/1‑150 (MQ=255)

                                                                                                                        gcTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCa                               <  2:864223/150‑1 (MQ=255)

                                                                                                                           cGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGAc                            <  2:1894057/150‑1 (MQ=255)

                                                                                                                            gATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACg                           >  1:5152905/1‑150 (MQ=255)

                                                                                                                            gATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACg                           >  1:506402/1‑150 (MQ=255)

                                                                                                                             aTCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGt                          >  1:3315946/1‑150 (MQ=255)

                                                                                                                              tCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGtt                         >  2:3734901/1‑150 (MQ=255)

                                                                                                                               cAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTc                        <  2:2212359/150‑1 (MQ=255)

                                                                                                                               cAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTc                        <  2:4008573/150‑1 (MQ=255)

                                                                                                                               cAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAAGCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTc                        <  2:4430019/150‑1 (MQ=255)

                                                                                                                                aGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCg                       >  2:1656705/1‑150 (MQ=255)

                                                                                                                                   ttACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCa                    >  2:1384374/1‑150 (MQ=255)

                                                                                                                                    tACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCaa                   >  1:1936974/1‑150 (MQ=255)

                                                                                                                                      ccTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATc                 <  2:327934/150‑1 (MQ=255)

                                                                                                                                       cTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCa                <  1:553798/150‑1 (MQ=255)

                                                                                                                                       cTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCa                <  2:4534507/150‑1 (MQ=255)

                                                                                                                                        tGCGCCCACCATCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCACTCAg               >  2:1823288/1‑150 (MQ=255)

                                                                                                                                         gcgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAgg              >  2:2389398/1‑150 (MQ=255)

                                                                                                                                          cgcCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGa             <  1:833768/150‑1 (MQ=255)

                                                                                                                                            cccACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCACTCAGGAAc           >  1:4588454/1‑150 (MQ=255)

                                                                                                                                             ccaccaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACa          >  2:4662897/1‑150 (MQ=255)

                                                                                                                                             ccaccaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACa          >  2:1443621/1‑150 (MQ=255)

                                                                                                                                                ccaTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACaaaa       <  2:4894427/150‑1 (MQ=255)

                                                                                                                                                  aTCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATg     <  1:2665459/150‑1 (MQ=255)

                                                                                                                                                  aTCGGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATg     <  1:245742/150‑1 (MQ=255)

                                                                                                                                                  aTCAGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATg     >  2:4178744/1‑150 (MQ=255)

                                                                                                                                                   tCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGa    <  2:3689345/150‑1 (MQ=255)

                                                                                                                                                     tGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATc  <  1:2086938/150‑1 (MQ=255)

                                                                                                                                                     tGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATc  <  1:4252888/150‑1 (MQ=255)

ACAGCACGGCTTTGATAATTTGCACCCAGGTGGTCGCCAGCATGCCGCCGAACAGGACGTACATCATCATCAGCACGCCGACCAGCACCACCGCAATGTGATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATC  >  CP009273/4274188‑4274486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`