Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,862 C→A 100% D480Y (GAT→TAT)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,8620CA100.0% 859.5 / ‑6.7 260D480Y (GAT→TAT) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (153/105);  total (155/105)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

TCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCT  >  CP009273/4275715‑4276008
                                                                                                                                                   |                                                                                                                                                  
tCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGc                                                                                                                                                  >  2:699958/1‑150 (MQ=255)
 ccccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGcc                                                                                                                                                 <  1:3205087/150‑1 (MQ=255)
 ccccAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGcc                                                                                                                                                 <  1:265003/150‑1 (MQ=255)
        cGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGc                                                                                                                                          >  1:1688419/1‑150 (MQ=255)
         ggTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCg                                                                                                                                         >  2:184577/1‑150 (MQ=255)
          gTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGt                                                                                                                                        <  2:4508314/150‑1 (MQ=255)
          gTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGt                                                                                                                                        <  2:380086/150‑1 (MQ=255)
           tGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTa                                                                                                                                       <  1:2017000/150‑1 (MQ=255)
           tGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTa                                                                                                                                       <  1:4653991/150‑1 (MQ=255)
             aCCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTAcg                                                                                                                                     >  2:3983924/1‑150 (MQ=255)
                ggAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACgcgc                                                                                                                                  >  2:3761063/1‑150 (MQ=255)
                     cGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGTAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAc                                                                                                                             >  1:2576697/1‑150 (MQ=255)
                      gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGAcc                                                                                                                            >  2:776315/1‑150 (MQ=255)
                        tCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCg                                                                                                                          <  1:5111784/150‑1 (MQ=255)
                         cAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCgg                                                                                                                         >  2:4684532/1‑150 (MQ=255)
                              cgtcgtCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAgg                                                                                                                    >  1:4388285/1‑150 (MQ=255)
                                 cgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt                                                                                                                 >  2:807032/1‑150 (MQ=255)
                                  gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAATCAGCGTACGCGCCTGACCCGGCCAGGAGTc                                                                                                                >  2:2491733/1‑150 (MQ=255)
                                  gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc                                                                                                                >  2:247348/1‑150 (MQ=255)
                                  gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc                                                                                                                <  2:4833567/150‑1 (MQ=255)
                                   tcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCg                                                                                                               <  1:3894436/150‑1 (MQ=255)
                                       cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                                                                                                           >  2:881832/1‑150 (MQ=255)
                                       cacGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGa                                                                                                           >  1:148492/1‑150 (MQ=255)
                                         cGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                         <  2:2925732/150‑1 (MQ=255)
                                         cGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCACTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtt                                                                                                         <  1:2730861/150‑1 (MQ=255)
                                          gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTa                                                                                                        <  1:3800084/150‑1 (MQ=255)
                                           cccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAc                                                                                                       <  1:2374235/150‑1 (MQ=255)
                                            ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAcc                                                                                                      <  1:3995059/150‑1 (MQ=255)
                                            ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAcc                                                                                                      <  2:1892433/150‑1 (MQ=255)
                                            ccGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTAcc                                                                                                      >  2:562791/1‑150 (MQ=255)
                                             cGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCa                                                                                                     <  1:360990/150‑1 (MQ=255)
                                              ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                    >  1:4461313/1‑150 (MQ=255)
                                              ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                    <  2:4202190/150‑1 (MQ=255)
                                              ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAg                                                                                                    <  2:3417843/150‑1 (MQ=255)
                                               gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAgg                                                                                                   <  1:4053179/150‑1 (MQ=255)
                                               gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAgg                                                                                                   >  2:3518271/1‑150 (MQ=255)
                                                 tATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCt                                                                                                 <  2:1683489/150‑1 (MQ=255)
                                                 tATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCt                                                                                                 <  2:4924951/150‑1 (MQ=255)
                                                  aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTa                                                                                                >  1:2413053/1‑150 (MQ=255)
                                                     cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCt                                                                                             <  1:4719821/150‑1 (MQ=255)
                                                      aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCtt                                                                                            <  2:2632901/150‑1 (MQ=255)
                                                         aaTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCgg                                                                                         >  1:5066761/1‑150 (MQ=255)
                                                          aTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGt                                                                                        >  1:3968294/1‑150 (MQ=255)
                                                           tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTg                                                                                       <  2:3453955/150‑1 (MQ=255)
                                                              ccATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcc                                                                                    >  1:4812982/1‑150 (MQ=255)
                                                               cATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGccc                                                                                   >  2:841356/1‑150 (MQ=255)
                                                                aTCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                  <  1:5012517/150‑1 (MQ=255)
                                                                aTCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                  >  1:4146090/1‑150 (MQ=255)
                                                                aTCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGcccc                                                                                  >  2:2744503/1‑150 (MQ=255)
                                                                  cTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCt                                                                                >  1:1826927/1‑150 (MQ=255)
                                                                     cATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                             >  2:3305855/1‑150 (MQ=255)
                                                                     cATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                             >  2:4279433/1‑150 (MQ=255)
                                                                     cATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                             >  2:5283859/1‑150 (MQ=255)
                                                                     cATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCa                                                                             <  2:2387160/150‑1 (MQ=255)
                                                                      aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAg                                                                            >  1:1412230/1‑150 (MQ=255)
                                                                              gcgcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTAc                                                                    <  1:3444011/150‑1 (MQ=255)
                                                                                gcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCt                                                                  >  2:1890603/1‑150 (MQ=255)
                                                                                gcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCt                                                                  <  2:3589053/150‑1 (MQ=255)
                                                                                 cgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCtt                                                                 <  1:1228720/150‑1 (MQ=255)
                                                                                  gcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTc                                                                >  2:1103363/1‑150 (MQ=255)
                                                                                      tCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTa                                                            >  1:3020675/1‑150 (MQ=255)
                                                                                        gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATc                                                          <  1:3288851/150‑1 (MQ=255)
                                                                                             tGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCa                                                     >  1:1046984/1‑150 (MQ=255)
                                                                                             tGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCa                                                     <  2:7405/150‑1 (MQ=255)
                                                                                               aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                   >  2:3351257/1‑150 (MQ=255)
                                                                                               aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                   >  1:2825756/1‑150 (MQ=255)
                                                                                                 aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgcgc                                                 >  2:2346259/1‑150 (MQ=255)
                                                                                                 aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgcgc                                                 >  2:54838/1‑150 (MQ=255)
                                                                                                  tACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGcc                                                <  2:4363518/150‑1 (MQ=255)
                                                                                                   aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCg                                               >  2:4606296/1‑150 (MQ=255)
                                                                                                   aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCg                                               >  1:857614/1‑150 (MQ=255)
                                                                                                      tatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGtt                                            <  1:2911592/150‑1 (MQ=255)
                                                                                                      tatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGACGGAGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTAACCTACGTTATCGACCAGCGCCGGtt                                            >  2:1219819/1‑150 (MQ=255)
                                                                                                       atTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCTGCCAGTAGTCGGTGATTACCAGGCTACCTTCGGTGTCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCTGTTg                                           <  2:2025639/150‑1 (MQ=255)
                                                                                                       atTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTg                                           <  2:272412/150‑1 (MQ=255)
                                                                                                       atTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTg                                           >  1:219620/1‑150 (MQ=255)
                                                                                                             gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGcc                                     <  2:621004/150‑1 (MQ=255)
                                                                                                             gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGcc                                     >  2:3036732/1‑150 (MQ=255)
                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                                >  2:3853169/1‑150 (MQ=255)
                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                                <  2:3655077/150‑1 (MQ=255)
                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaaga                                >  2:143428/1‑150 (MQ=255)
                                                                                                                   ggAGAAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaagaa                               >  2:5220534/1‑150 (MQ=255)
                                                                                                                    gagaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAAc                              <  2:1303126/150‑1 (MQ=255)
                                                                                                                    gagaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAAc                              <  1:320047/150‑1 (MQ=255)
                                                                                                                      gaAGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACgg                            >  1:3250843/1‑150 (MQ=255)
                                                                                                                        aGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGAc                          <  2:917320/150‑1 (MQ=255)
                                                                                                                        aGTAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGAc                          <  2:5024520/150‑1 (MQ=255)
                                                                                                                          tAGGTCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgt                        <  1:4334051/150‑1 (MQ=255)
                                                                                                                              tCTGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTg                    >  1:1279528/1‑150 (MQ=255)
                                                                                                                                tGTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGcc                  >  2:2929577/1‑150 (MQ=255)
                                                                                                                                 gTTCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCg                 >  1:4538071/1‑150 (MQ=255)
                                                                                                                                  ttCAAAACGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGa                >  2:1388057/1‑150 (MQ=255)
                                                                                                                                  ttCAAAACGATCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGa                >  2:4109022/1‑150 (MQ=255)
                                                                                                                                    cAAAACGATCGAGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGTTCAAAACCAGGCTACCTACGGTGGCCCCCTCCAGCGGGTTAACTTCGTTATCGACCATCGCCGGATTCACGCCGAAGAAAGGACGTGTTTCCCAAc              >  1:1360990/1‑150 (MQ=255)
                                                                                                                                       aaCGTTCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCgg           >  1:4763322/1‑150 (MQ=255)
                                                                                                                                            tCGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTc      <  1:750857/150‑1 (MQ=255)
                                                                                                                                             cGTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCa     >  2:5175050/1‑150 (MQ=255)
                                                                                                                                              gTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAg    >  2:3905454/1‑150 (MQ=255)
                                                                                                                                              gTGATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAg    >  2:1615699/1‑150 (MQ=255)
                                                                                                                                                gATAGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCt  >  1:4863611/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCT  >  CP009273/4275715‑4276008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑