Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,279,816 G→T 72.8% A27A (GCG→GCT)  nrfC → formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,279,8160GT72.8% 398.0 / 290.4 332A27A (GCG→GCT) nrfCformate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base G (39/51);  new base T (117/124);  total (156/176)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

Only 100 of 337 total aligned reads displayed.

GCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  CP009273/4279669‑4279965
                                                                                                                                                   |                                                                                                                                                     
gCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTaa                                                                                                                                                     <  2:645520/150‑1 (MQ=255)
   acaGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTNGTGGCGAAAAc                                                                                                                                                  <  1:2765111/150‑1 (MQ=255)
   acaGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAAc                                                                                                                                                  <  1:295143/150‑1 (MQ=255)
    caGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACg                                                                                                                                                 <  2:3555845/150‑1 (MQ=255)
       cGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTg                                                                                                                                              <  2:1703663/150‑1 (MQ=255)
        gATCAGCGCACCAATCCGAACTATAACCCGGCGTCTGATCCGTTGCTTAAGGAGCAGCCAAGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGAGGCTAAAACGTTGa                                                                                                                                             >  2:2119448/1‑150 (MQ=255)
         aTCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGaa                                                                                                                                            >  1:4413056/1‑150 (MQ=255)
         aTCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGaa                                                                                                                                            >  1:2071310/1‑150 (MQ=255)
          tCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAat                                                                                                                                           <  1:4542991/150‑1 (MQ=255)
            aGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAatat                                                                                                                                         >  2:2710640/1‑150 (MQ=255)
             gcgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGACTATc                                                                                                                                        >  1:1915578/1‑150 (MQ=255)
             gcgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCCAAAACGTTGAATATc                                                                                                                                        <  1:941551/150‑1 (MQ=255)
              cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                                       >  2:1836848/1‑150 (MQ=255)
              cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCa                                                                                                                                       >  1:18386/1‑150 (MQ=255)
                cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAAt                                                                                                                                     <  1:2266723/150‑1 (MQ=255)
                cACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAAt                                                                                                                                     <  1:1350933/150‑1 (MQ=255)
                   cAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATggg                                                                                                                                  <  1:4285908/150‑1 (MQ=255)
                    aaTCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATgggg                                                                                                                                 >  2:4558006/1‑150 (MQ=255)
                    aaTCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATgggg                                                                                                                                 >  2:1205566/1‑150 (MQ=255)
                    aaTCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATgggg                                                                                                                                 <  2:2007818/150‑1 (MQ=255)
                      tCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTg                                                                                                                               >  2:2085686/1‑150 (MQ=255)
                       ccGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGc                                                                                                                              <  2:4948766/150‑1 (MQ=255)
                          aaCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCACTGGGGTGCGtt                                                                                                                           >  2:1101117/1‑150 (MQ=255)
                           aCTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTa                                                                                                                          >  2:35284/1‑150 (MQ=255)
                                 aCCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCAt                                                                                                                    <  2:3210151/150‑1 (MQ=255)
                                    cGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGt                                                                                                                 >  1:2440747/1‑150 (MQ=255)
                                    cGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGt                                                                                                                 <  2:2029414/150‑1 (MQ=255)
                                       cGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCa                                                                                                              >  1:4069101/1‑150 (MQ=255)
                                        gTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCAt                                                                                                             >  2:456651/1‑150 (MQ=255)
                                        gTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCAt                                                                                                             >  1:2132474/1‑150 (MQ=255)
                                        gTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCAt                                                                                                             >  1:630959/1‑150 (MQ=255)
                                         tCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATg                                                                                                            <  2:557567/150‑1 (MQ=255)
                                          cTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGa                                                                                                           <  1:3970063/150‑1 (MQ=255)
                                           tGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGAc                                                                                                          >  2:1321709/1‑150 (MQ=255)
                                           tGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGAc                                                                                                          >  2:1352821/1‑150 (MQ=255)
                                              tCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAg                                                                                                       <  1:3841159/150‑1 (MQ=255)
                                                cGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTc                                                                                                     <  2:3054976/150‑1 (MQ=255)
                                                 gTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCg                                                                                                    >  1:431797/1‑150 (MQ=255)
                                                 gTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCg                                                                                                    >  1:3419504/1‑150 (MQ=255)
                                                   tGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTATGGCATGGTGCATGACGAGTCGtt                                                                                                  >  1:984816/1‑150 (MQ=255)
                                                    gCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTa                                                                                                 >  1:4771702/1‑150 (MQ=255)
                                                     cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTAt                                                                                                <  2:4141882/150‑1 (MQ=255)
                                                     cTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTAt                                                                                                <  2:1420961/150‑1 (MQ=255)
                                                         aGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCAt                                                                                            <  1:1952393/150‑1 (MQ=255)
                                                           gAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCg                                                                                          >  2:2383913/1‑150 (MQ=255)
                                                                gcCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGt                                                                                     >  2:3803776/1‑150 (MQ=255)
                                                                  cATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTAc                                                                                   <  2:3152474/150‑1 (MQ=255)
                                                                    tGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCg                                                                                 <  1:2615929/150‑1 (MQ=255)
                                                                     gACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGc                                                                                >  2:2868688/1‑150 (MQ=255)
                                                                       ccTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCt                                                                              <  2:1251235/150‑1 (MQ=255)
                                                                       ccTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCt                                                                              >  2:3477379/1‑150 (MQ=255)
                                                                        cTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                             <  2:673918/150‑1 (MQ=255)
                                                                        cTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTg                                                                             <  1:3144151/150‑1 (MQ=255)
                                                                           gTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGAATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTAt                                                                          <  2:2220820/150‑1 (MQ=255)
                                                                            tctcGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATg                                                                         <  1:2831279/150‑1 (MQ=255)
                                                                               cgtcgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGAt                                                                      <  1:3776015/150‑1 (MQ=255)
                                                                               cgtcgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGAt                                                                      <  2:4631355/150‑1 (MQ=255)
                                                                                  cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAACACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCt                                                                   <  2:483918/150‑1 (MQ=255)
                                                                                  cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCt                                                                   >  1:1986400/1‑150 (MQ=255)
                                                                                  cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGACAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCt                                                                   >  2:2927118/1‑150 (MQ=255)
                                                                                  cgCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCt                                                                   <  2:4268609/150‑1 (MQ=255)
                                                                                   gCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCtt                                                                  >  2:4317858/1‑150 (MQ=255)
                                                                                         ttCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCggg                                                            <  1:2399903/150‑1 (MQ=255)
                                                                                              aCCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTg                                                       <  2:2772042/150‑1 (MQ=255)
                                                                                                cggcgtcggcgtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGaa                                                     <  2:4976163/150‑1 (MQ=255)
                                                                                                          gtGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCg                                           >  1:1080528/1‑150 (MQ=255)
                                                                                                              tGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTTTGCATCGGCTGTACCGCCGGGATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGTAg                                       >  2:875515/1‑150 (MQ=255)
                                                                                                               ggCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAgg                                      >  2:4474799/1‑150 (MQ=255)
                                                                                                                gCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGc                                     <  2:2350471/150‑1 (MQ=255)
                                                                                                                gCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGc                                     <  1:4897363/150‑1 (MQ=255)
                                                                                                                 cAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCg                                    <  2:1971349/150‑1 (MQ=255)
                                                                                                                  aGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGt                                   <  2:3258194/150‑1 (MQ=255)
                                                                                                                   gCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtc                                  <  2:5267986/150‑1 (MQ=255)
                                                                                                                    ccGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtct                                 <  1:517607/150‑1 (MQ=255)
                                                                                                                     cGTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtctc                                >  1:4164438/1‑150 (MQ=255)
                                                                                                                     cGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGtctc                                >  1:720062/1‑150 (MQ=255)
                                                                                                                      gTCAGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTcg                               <  1:1497429/150‑1 (MQ=255)
                                                                                                                         aGCGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCgcgc                            >  2:4997156/1‑150 (MQ=255)
                                                                                                                           cGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATTCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTAGCGCCt                          >  1:35809/1‑150 (MQ=255)
                                                                                                                           cGGCACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCt                          <  2:936648/150‑1 (MQ=255)
                                                                                                                              cACCGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGAc                       >  2:4101549/1‑150 (MQ=255)
                                                                                                                                 cGCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGat                    <  2:1583025/150‑1 (MQ=255)
                                                                                                                                  gCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatt                   >  2:3940896/1‑150 (MQ=255)
                                                                                                                                  gCAGGGCGCGTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGatt                   <  1:3728050/150‑1 (MQ=255)
                                                                                                                                     gggCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGattatt                <  1:4714608/150‑1 (MQ=255)
                                                                                                                                          cgTGTTGGCGAATACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTAGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTTAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAg           <  1:5302710/150‑1 (MQ=255)
                                                                                                                                          cgTGGTGGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAg           <  2:2510044/150‑1 (MQ=255)
                                                                                                                                          cgTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAg           <  1:2688395/150‑1 (MQ=255)
                                                                                                                                           gtggtgGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGc          >  2:5173740/1‑150 (MQ=255)
                                                                                                                                           gtggtgGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGc          <  1:1014225/150‑1 (MQ=255)
                                                                                                                                           gtggtgGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGc          >  2:3392004/1‑150 (MQ=255)
                                                                                                                                           gtggtgGCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACAGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGc          >  2:3672244/1‑150 (MQ=255)
                                                                                                                                            tggtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCg         <  2:1915578/150‑1 (MQ=255)
                                                                                                                                            tggtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCAGTATGGATGCTTGTCGGGAAGAGAACAAGATGCCGGAAGGCGTCTCGCCCCAGACGATTATTCGTATCg         >  1:4017423/1‑150 (MQ=255)
                                                                                                                                              gtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAg       <  1:810571/150‑1 (MQ=255)
                                                                                                                                              gtggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAg       <  1:1598489/150‑1 (MQ=255)
                                                                                                                                              gtggCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCAGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAg       >  2:613971/1‑150 (MQ=255)
                                                                                                                                               tggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGc      <  2:2240061/150‑1 (MQ=255)
                                                                                                                                                ggCTAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATACATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGcc     <  2:4798228/150‑1 (MQ=255)
                                                                                                                                                   gAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCa  <  2:3885036/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCGCGTGGTGGCGAAAACGTTGAATATCAATGGGGTGCGTTACGGCATGGTGCATGACGAGTCGTTATGCATCGGCTGTACCGCCTGTATGGATGCTTGTCGGGAAGTGAACAAGGTGCCGGAAGGCGTCTCGCGCCTGACGATTATTCGTAGCGAGCCGCA  >  CP009273/4279669‑4279965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑