Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,288,086 G→T 100% F366L (TTC→TTA)  fdhF ← formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,288,0860GT99.7% 961.9 / ‑4.3 294F366L (TTC→TTA) fdhFformate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (129/164);  total (129/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 294 total aligned reads displayed.

TACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287939‑4288231
                                                                                                                                                   |                                                                                                                                                 
  cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAtt                                                                                                                                               <  2:4225098/150‑1 (MQ=255)
  cTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAAtt                                                                                                                                               <  2:3661709/150‑1 (MQ=255)
     ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                            >  2:2055547/1‑150 (MQ=255)
     ccGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtct                                                                                                                                            <  1:2216537/150‑1 (MQ=255)
      cGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTtctc                                                                                                                                           <  1:2917363/150‑1 (MQ=255)
            cTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGtt                                                                                                                                     >  1:2030726/1‑150 (MQ=255)
              ccGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAg                                                                                                                                   <  2:2241191/150‑1 (MQ=255)
              ccGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAg                                                                                                                                   <  2:2490071/150‑1 (MQ=255)
                  gTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCgg                                                                                                                               >  2:3132792/1‑150 (MQ=255)
                       tttGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCtt                                                                                                                          <  1:3701085/150‑1 (MQ=255)
                          gTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCa                                                                                                                       <  1:2700981/150‑1 (MQ=255)
                           tAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCAc                                                                                                                      >  1:1037049/1‑150 (MQ=255)
                            aGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACg                                                                                                                     >  2:3013505/1‑150 (MQ=255)
                             gCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGt                                                                                                                    <  2:3757804/150‑1 (MQ=255)
                                gATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                                 <  1:2540252/150‑1 (MQ=255)
                                gATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                                 <  1:2639833/150‑1 (MQ=255)
                                gATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACt                                                                                                                 >  1:3010751/1‑150 (MQ=255)
                                gATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTACACGTACt                                                                                                                 >  1:4580207/1‑150 (MQ=255)
                                 aTCTTCGCCCATAATGTACGCGGCAAGCTCTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTg                                                                                                                <  2:2707343/150‑1 (MQ=255)
                                     tCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTGTCACGTACTGGTAt                                                                                                            >  2:2422269/1‑150 (MQ=255)
                                      cGNCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                           >  1:1921150/1‑150 (MQ=255)
                                      cGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                           <  1:4394318/150‑1 (MQ=255)
                                      cGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATc                                                                                                           >  1:2522344/1‑150 (MQ=255)
                                       gCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATcc                                                                                                          >  2:1549618/1‑150 (MQ=255)
                                       gCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATcc                                                                                                          <  1:1122368/150‑1 (MQ=255)
                                        cccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATccc                                                                                                         >  1:4475196/1‑150 (MQ=255)
                                         ccATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCg                                                                                                        >  1:4167923/1‑150 (MQ=255)
                                             aaTGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATa                                                                                                    >  1:3880167/1‑150 (MQ=255)
                                              aTGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATAc                                                                                                   >  2:1063540/1‑150 (MQ=255)
                                               tGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACg                                                                                                  <  1:3160234/150‑1 (MQ=255)
                                                   cgcgGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATc                                                                                              <  1:710505/150‑1 (MQ=255)
                                                      ggCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCgg                                                                                           <  1:1862652/150‑1 (MQ=255)
                                                       gcacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGc                                                                                          <  1:3695787/150‑1 (MQ=255)
                                                        cacgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCAGGTATGCGCTGGCAGGATGTCCACGCCCCAGGCTTTGGCTAATTACTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATAATTATCCGGCa                                                                                         >  1:1746/1‑150 (MQ=255)
                                                          cgcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgc                                                                                       <  1:967368/150‑1 (MQ=255)
                                                           gcacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcg                                                                                      >  1:1024235/1‑150 (MQ=255)
                                                            cacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTCTCCCGGATACGTATCCGGCAgcgc                                                                                     >  2:3417894/1‑150 (MQ=255)
                                                            cacTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgc                                                                                     <  2:4533617/150‑1 (MQ=255)
                                                             acTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcg                                                                                    >  1:605899/1‑150 (MQ=255)
                                                              cTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAgcgcgc                                                                                   >  2:17226/1‑150 (MQ=255)
                                                                    cATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                             <  1:2270421/150‑1 (MQ=255)
                                                                    cATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                             <  2:1638214/150‑1 (MQ=255)
                                                                    cATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCatat                                                                             >  1:478918/1‑150 (MQ=255)
                                                                         gctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGTCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCAGGATACGTATCCGGCAGCGCGCCCATATCGCAg                                                                        <  2:899348/150‑1 (MQ=255)
                                                                         gctgcgcGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAg                                                                        <  2:3069694/150‑1 (MQ=255)
                                                                              gcgGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                   >  1:1626830/1‑150 (MQ=255)
                                                                              gcgGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAcc                                                                   <  1:2825464/150‑1 (MQ=255)
                                                                               cgGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCAccc                                                                  <  2:5184597/150‑1 (MQ=255)
                                                                                ggTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCt                                                                 <  2:2323821/150‑1 (MQ=255)
                                                                                 gTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTTGCTAATTTCTCGCGGTTAGCCGGATCTTTCACTTACTGGTATCCCTGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTg                                                                <  1:4231556/150‑1 (MQ=255)
                                                                                 gTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTg                                                                <  1:5048620/150‑1 (MQ=255)
                                                                                 gTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTg                                                                >  2:488188/1‑150 (MQ=255)
                                                                                 gTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTg                                                                <  1:4032686/150‑1 (MQ=255)
                                                                                   gCGGCAGCTCGCTGATGCGATAGACGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGaa                                                              <  1:3724899/150‑1 (MQ=255)
                                                                                    cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAAc                                                             <  1:783589/150‑1 (MQ=255)
                                                                                    cGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAAc                                                             <  1:8336/150‑1 (MQ=255)
                                                                                       cAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgtt                                                          <  1:4899469/150‑1 (MQ=255)
                                                                                        aGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttg                                                         >  2:544142/1‑150 (MQ=255)
                                                                                         gCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgt                                                        <  1:4288107/150‑1 (MQ=255)
                                                                                          cTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACgttgtt                                                       <  1:2386584/150‑1 (MQ=255)
                                                                                            cGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCt                                                     <  2:3896891/150‑1 (MQ=255)
                                                                                             gCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTg                                                    <  1:2807697/150‑1 (MQ=255)
                                                                                              cTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTGTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGACCGTTGTTCTGa                                                   >  2:3291246/1‑150 (MQ=255)
                                                                                               tGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGAc                                                  <  1:1328535/150‑1 (MQ=255)
                                                                                                   gCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACg                                              >  2:1456602/1‑150 (MQ=255)
                                                                                                      aTAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAc                                           >  1:3052327/1‑150 (MQ=255)
                                                                                                      aTAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAc                                           >  2:2865320/1‑150 (MQ=255)
                                                                                                       tAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                          >  1:4765580/1‑150 (MQ=255)
                                                                                                       tAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAAcc                                          >  2:3223730/1‑150 (MQ=255)
                                                                                                            ggTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATCTCGCAGGCACCCTGAACGTTGTTCTGACCCCGAACCGGGtt                                     >  2:2184279/1‑150 (MQ=255)
                                                                                                            ggTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGtt                                     >  1:5294243/1‑150 (MQ=255)
                                                                                                            ggTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGtt                                     <  1:1438330/150‑1 (MQ=255)
                                                                                                            ggTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGtt                                     <  1:1533476/150‑1 (MQ=255)
                                                                                                            ggTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGtt                                     >  2:1138269/1‑150 (MQ=255)
                                                                                                             gTATGCGCTGGCATGCTTTACACGACCCAGTCTTTGGCTTATTTCTCGCGGTTAGCCGGATCTTTCACTTACTGGTATCCCGGATAAGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTa                                    <  1:1481248/150‑1 (MQ=255)
                                                                                                             gTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTa                                    >  2:2779790/1‑150 (MQ=255)
                                                                                                             gTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTa                                    >  2:4076442/1‑150 (MQ=255)
                                                                                                               aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCTGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAac                                  >  2:4362289/1‑150 (MQ=255)
                                                                                                               aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAac                                  <  2:4146363/150‑1 (MQ=255)
                                                                                                               aTGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAac                                  <  2:1225169/150‑1 (MQ=255)
                                                                                                                  cgcTGGCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACAcc                               <  1:3548446/150‑1 (MQ=255)
                                                                                                                      ggCAGGCTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCa                           >  1:4675642/1‑150 (MQ=255)
                                                                                                                            cTTTCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGc                     >  2:3340635/1‑150 (MQ=255)
                                                                                                                             tttCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCt                    <  1:3670235/150‑1 (MQ=255)
                                                                                                                             tttCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCt                    >  2:4001082/1‑150 (MQ=255)
                                                                                                                               tCCACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTa                  <  2:3516594/150‑1 (MQ=255)
                                                                                                                                ccACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAc                 >  2:3830129/1‑150 (MQ=255)
                                                                                                                                ccACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAc                 <  2:4677808/150‑1 (MQ=255)
                                                                                                                                 cACGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTAcc                <  2:2641687/150‑1 (MQ=255)
                                                                                                                                  aCGCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCg               >  1:5236760/1‑150 (MQ=255)
                                                                                                                                    gCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAg             >  1:3486807/1‑150 (MQ=255)
                                                                                                                                    gCCCCAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAg             <  2:5031535/150‑1 (MQ=255)
                                                                                                                                    gCCCCAGGCTTTGGCTAATGTCTCGCGGTTAGCCGGATCTTTCACGTCCTGGGATCCCGGCTACGTATCCGGCAGCGCGCCCCTAGCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAg             <  2:1275040/150‑1 (MQ=255)
                                                                                                                                      cccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGt           >  2:2661861/1‑150 (MQ=255)
                                                                                                                                       ccAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGtt          <  2:5072820/150‑1 (MQ=255)
                                                                                                                                        cAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa         >  2:3479167/1‑150 (MQ=255)
                                                                                                                                        cAGGCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTa         >  2:4808129/1‑150 (MQ=255)
                                                                                                                                          ggCTTTGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTAcc       <  1:3226839/150‑1 (MQ=255)
                                                                                                                                               tGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCa  <  1:1176878/150‑1 (MQ=255)
                                                                                                                                               tGGCTAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCa  <  1:337881/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TACTGCCGACAGCTCCGCGTCAGTTTGTAGCGGATCTTCGCCCATAATGTACGCGGCACGCACTTCGCCATGCGCTGCGCGGTGCGGCAGCTCGCTGATGCGATAGCCGGTATGCGCTGGCAGGCTTTCCACGCCCCAGGCTTTGGCGAATTTCTCGCGGTTAGCCGGATCTTTCACGTACTGGTATCCCGGATACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCA  >  CP009273/4287939‑4288231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑