Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,329,774 C→A 100% D189Y (GAC→TAC)  adiA ← arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,329,7740CA99.7% 850.0 / ‑6.3 291D189Y (GAC→TAC) adiAarginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (4/2);  major base A (156/122);  minor base T (0/7);  total (160/131)
Fisher's exact test for biased strand distribution p-value = 3.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 291 total aligned reads displayed.

GATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCG  >  CP009273/4329628‑4329920
                                                                                                                                                  |                                                                                                                                                  
gatgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGt                                                                                                                                                 <  2:5186858/150‑1 (MQ=255)
gatgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGt                                                                                                                                                 >  1:460607/1‑150 (MQ=255)
gatgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGt                                                                                                                                                 <  1:1534656/150‑1 (MQ=255)
 atgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTg                                                                                                                                                >  2:1131990/1‑150 (MQ=255)
 atgGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTg                                                                                                                                                <  2:3543225/150‑1 (MQ=255)
     tGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGa                                                                                                                                            >  1:4584507/1‑150 (MQ=255)
      gCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCAAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGaa                                                                                                                                           <  2:3191066/150‑1 (MQ=255)
       cGGTGAGAGCCGGACGTACCGACGACTACCGACCAGGCGCGCTCGGCACCACATCCGCGTGCGGCATATATTTCGCGTACGCCAACTGCGCCAGTATGGTCACGCACAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAAc                                                                                                                                          <  2:881690/150‑1 (MQ=255)
         gTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAg                                                                                                                                        >  1:858524/1‑150 (MQ=255)
         gTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAg                                                                                                                                        <  2:4811771/150‑1 (MQ=255)
                  ggAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCAcc                                                                                                                               <  2:862207/150‑1 (MQ=255)
                       tACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCAtagt                                                                                                                          >  1:5157731/1‑150 (MQ=255)
                          cgacgaCTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATagtagt                                                                                                                       >  2:3923083/1‑150 (MQ=255)
                             cgacTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCAt                                                                                                                    <  1:4213928/150‑1 (MQ=255)
                               acTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATgg                                                                                                                  >  1:2366807/1‑150 (MQ=255)
                               acTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATgg                                                                                                                  <  2:1559998/150‑1 (MQ=255)
                                cgaCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                                 <  1:2019332/148‑1 (MQ=255)
                                cTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                                 <  2:2762855/150‑1 (MQ=255)
                                cTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGt                                                                                                                 <  2:2962904/150‑1 (MQ=255)
                                 tACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTa                                                                                                                >  2:1148668/1‑150 (MQ=255)
                                  aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                               >  1:580453/1‑150 (MQ=255)
                                  aCCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                               >  2:4570573/1‑150 (MQ=255)
                                  aCCGACCAGGAGCGATCGGCACCAAAAACGCGTGCGTCAAATTTTTCGCTTTCGCCAAATGCTCCAGTATGGTCAAGCAAAGAACCGAGGGAAGATCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAg                                                                                                               >  1:1670164/1‑150 (MQ=255)
                                     gACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGaaa                                                                                                            >  2:2126042/1‑150 (MQ=255)
                                      aCCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAAc                                                                                                           <  1:135133/150‑1 (MQ=255)
                                       ccAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACg                                                                                                          <  1:2294063/150‑1 (MQ=255)
                                          ggAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGGTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTcc                                                                                                       >  1:1724554/1‑150 (MQ=255)
                                          ggAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTcc                                                                                                       <  1:3449144/150‑1 (MQ=255)
                                           gAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcg                                                                                                      >  1:29841/1‑150 (MQ=255)
                                           gAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcg                                                                                                      >  2:4899955/1‑150 (MQ=255)
                                           gAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcg                                                                                                      <  2:500578/150‑1 (MQ=255)
                                            aGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcgg                                                                                                     >  2:3554795/1‑150 (MQ=255)
                                               gATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                  >  1:3955617/1‑150 (MQ=255)
                                               gATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCcggcgg                                                                                                  >  2:4154318/1‑150 (MQ=255)
                                                   ggCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCCTGGTAGAAACGTCCGGCGGgtgt                                                                                              >  2:3191439/1‑150 (MQ=255)
                                                    gCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGtt                                                                                             <  2:2288995/150‑1 (MQ=255)
                                                     cACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGttt                                                                                            <  1:1138578/150‑1 (MQ=255)
                                                       ccAACTCCGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTg                                                                                          <  1:2895395/150‑1 (MQ=255)
                                                       ccAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTg                                                                                          >  1:4259153/1‑150 (MQ=255)
                                                           atccgcgTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTAGGGTCACGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATCGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaa                                                                                      <  1:2482702/147‑1 (MQ=255)
                                                           aTACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaa                                                                                      >  1:1398706/1‑150 (MQ=255)
                                                             aCGCGTGCGACATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTaaaa                                                                                    >  1:1005035/1‑150 (MQ=255)
                                                              cgcgTGCGGCATATTTTTCGCTTTAGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAAc                                                                                   <  1:4989166/150‑1 (MQ=255)
                                                                cgTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCa                                                                                 >  1:4452953/1‑150 (MQ=255)
                                                                 gTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCaa                                                                                >  2:3064164/1‑150 (MQ=255)
                                                                     ggCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGACGTTCGTTCGATGCCCATGTTGGTGCGGAACATATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgcc                                                                            <  1:1636596/150‑1 (MQ=255)
                                                                       cATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAAcgccgc                                                                          >  2:1746244/1‑150 (MQ=255)
                                                                        atatTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACgccgcc                                                                         >  2:2638263/1‑150 (MQ=255)
                                                                             tttcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtggt                                                                    >  1:5278052/1‑150 (MQ=255)
                                                                               tcgctttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCtggtgg                                                                  >  1:1901862/1‑150 (MQ=255)
                                                                                  cttacgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCCAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGGAGAAACGTCCGGCGGGTGTGTTGGTACAACCAACGCCGCCCTGGTGGCCt                                                               >  1:1062869/1‑150 (MQ=255)
                                                                                   tttcgcCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTg                                                              <  1:5223555/150‑1 (MQ=255)
                                                                                        ccAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCt                                                         <  2:2747485/150‑1 (MQ=255)
                                                                                         cAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTg                                                        >  2:2001916/1‑150 (MQ=255)
                                                                                          aaaTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGc                                                       >  2:4064646/1‑150 (MQ=255)
                                                                                            aTGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGccc                                                     <  2:2421983/150‑1 (MQ=255)
                                                                                             tGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCa                                                    <  1:4425435/150‑1 (MQ=255)
                                                                                             tGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCa                                                    <  2:2389872/150‑1 (MQ=255)
                                                                                                gcCAGTATGGTCAAGAAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGa                                                 <  1:709959/150‑1 (MQ=255)
                                                                                                 ccAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGaa                                                <  2:2203226/150‑1 (MQ=255)
                                                                                                  cAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAat                                               <  2:4431432/150‑1 (MQ=255)
                                                                                                  cAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAat                                               >  2:3568874/1‑150 (MQ=255)
                                                                                                   aGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAata                                              >  1:2832051/1‑150 (MQ=255)
                                                                                                     tATGGTCAAGCAACGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATCGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATAtt                                            <  2:3873328/150‑1 (MQ=255)
                                                                                                       tGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCa                                          <  2:1594924/150‑1 (MQ=255)
                                                                                                          tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTATAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                       <  2:2456952/150‑1 (MQ=255)
                                                                                                          tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                       >  1:2191544/1‑150 (MQ=255)
                                                                                                          tCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATgg                                       <  1:3510742/150‑1 (MQ=255)
                                                                                                           cAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGa                                      >  1:3267280/1‑150 (MQ=255)
                                                                                                              gCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGt                                   >  1:1206171/1‑150 (MQ=255)
                                                                                                               cAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                  >  2:4362658/1‑150 (MQ=255)
                                                                                                               cAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                  <  1:3923083/150‑1 (MQ=255)
                                                                                                               cAAAGAACCGAGGGAAGTTCGGTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGGCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTc                                  <  2:2903581/150‑1 (MQ=255)
                                                                                                                aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                                 >  2:367937/1‑150 (MQ=255)
                                                                                                                aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                                 >  2:1209593/1‑150 (MQ=255)
                                                                                                                aaaGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCa                                 >  2:3611175/1‑150 (MQ=255)
                                                                                                                  aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                               <  1:3839704/150‑1 (MQ=255)
                                                                                                                  aGAACCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACt                               >  1:4511022/1‑150 (MQ=255)
                                                                                                                    aaCCGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACtat                             >  2:4628502/1‑150 (MQ=255)
                                                                                                                       cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                          >  1:5299516/1‑150 (MQ=255)
                                                                                                                       cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                          >  2:2565958/1‑150 (MQ=255)
                                                                                                                       cGAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAtt                          >  1:332351/1‑150 (MQ=255)
                                                                                                                        gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCTGGAATATTCATGGATGTCACTATAttt                         >  1:3072107/1‑150 (MQ=255)
                                                                                                                        gAGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATAttt                         <  2:4554587/150‑1 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGTTGCCCTTTTAGGTGCGGAACAGATTTTCACCTTAGTAGTCTTGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCTCTATATTtc                        <  1:262994/150‑1 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGATGCCCATGTATGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtc                        >  2:3790091/1‑150 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTCTATTtc                        >  2:1042245/1‑150 (MQ=255)
                                                                                                                         aGGGAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAAAGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtc                        >  1:5106211/1‑150 (MQ=255)
                                                                                                                          gggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtca                       >  2:3416063/1‑150 (MQ=255)
                                                                                                                           ggAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcat                      >  1:1463260/1‑150 (MQ=255)
                                                                                                                            gAAGTTCGTTCGATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTtcatc                     >  2:279970/1‑150 (MQ=255)
                                                                                                                                  cgttcgttGCCATTGTTGGTGCGGAACATATTTTCACAATAGTTGTATTGTTATAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcg               <  1:4031243/150‑1 (MQ=255)
                                                                                                                                   gttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcgc              >  2:3463705/1‑150 (MQ=255)
                                                                                                                                   gttcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAgcgcgc              >  2:1781075/1‑150 (MQ=255)
                                                                                                                                     tcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTg            <  1:3793812/150‑1 (MQ=255)
                                                                                                                                     tcgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTg            <  2:580453/150‑1 (MQ=255)
                                                                                                                                      cgATGCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGa           >  2:470386/1‑150 (MQ=255)
                                                                                                                                          gCCCATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAg       <  2:460607/150‑1 (MQ=255)
                                                                                                                                           cccATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGt      >  1:3913275/1‑150 (MQ=255)
                                                                                                                                             cATGTAGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTgg    >  1:3564814/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GATGGTGCGGTTAGAGCCGGAAGTACCGACGACTACCGACCAGGAGCGATCGGCACCAAATACGCGTGCGGCATATTTTTCGCTTTCGCCAAATGCGCCAGTATGGTCAAGCAAAGAACCGAGGGAAGTTCGTTCGATGCCCATGTCGGTGCGGAACAGATTTTCACCATAGTAGTCATGGTAGAAACGTCCGGCGGGTGTTTTGGTAAAACCAACGCCGCCCTGGTGGCCTGGCGCTGCCCAGGAATATTCATGGATGTCACTATATTTCATCAGCGCGCTGAACAGTGGCG  >  CP009273/4329628‑4329920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑