Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,350,223 G→A 100% A510V (GCT→GTT)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,350,2230GA98.8% 1344.9 / 2.2 409A510V (GCT→GTT) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (203/201);  total (205/204)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 409 total aligned reads displayed.

GATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATT  >  CP009273/4350076‑4350369
                                                                                                                                                   |                                                                                                                                                  
gATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACa                                                                                                                                                  <  1:2636856/150‑1 (MQ=255)
  ttGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAg                                                                                                                                                <  1:4998871/150‑1 (MQ=255)
       tGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAAtt                                                                                                                                           <  1:1350953/150‑1 (MQ=255)
              ctctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgag                                                                                                                                    >  1:2235251/1‑150 (MQ=255)
              ctctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgag                                                                                                                                    <  2:3285679/150‑1 (MQ=255)
               tctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgaga                                                                                                                                   <  2:3102024/150‑1 (MQ=255)
               tctGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCgaga                                                                                                                                   >  1:2057287/1‑150 (MQ=255)
                   gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAg                                                                                                                               <  1:2809637/150‑1 (MQ=255)
                   gTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAg                                                                                                                               >  2:1333334/1‑150 (MQ=255)
                      aaaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTa                                                                                                                            >  1:1123601/1‑150 (MQ=255)
                      aaaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTa                                                                                                                            <  1:4532356/150‑1 (MQ=255)
                      aaaTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTa                                                                                                                            <  2:1460574/150‑1 (MQ=255)
                        aTTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTaca                                                                                                                          >  1:5236290/1‑150 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGtactac                                                                                                                        >  2:4774535/1‑150 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaac                                                                                                                        <  1:4052468/150‑1 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaac                                                                                                                        >  2:3609144/1‑150 (MQ=255)
                          ttATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTacaac                                                                                                                        >  2:4371946/1‑150 (MQ=255)
                           tATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATCAGGTacaaca                                                                                                                       >  1:492965/1‑150 (MQ=255)
                            aTGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACAt                                                                                                                      <  1:833592/150‑1 (MQ=255)
                                aataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAgg                                                                                                                  >  1:2612204/1‑150 (MQ=255)
                                 ataaAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAATTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGa                                                                                                                 >  2:2954866/1‑150 (MQ=255)
                                     aaaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAg                                                                                                             >  1:1948128/1‑150 (MQ=255)
                                      aaTTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGa                                                                                                            <  1:4149652/150‑1 (MQ=255)
                                        ttATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTGACTAATTCTGAAACAAGAACTTTGTCGAGATAAGGTACAACAGAAGGACCAGAAg                                                                                                          >  2:1194621/1‑150 (MQ=255)
                                           tGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCt                                                                                                       <  1:4713435/150‑1 (MQ=255)
                                            gCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTg                                                                                                      >  2:1565138/1‑150 (MQ=255)
                                             cGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTgg                                                                                                     >  1:3465048/1‑150 (MQ=255)
                                              ggCAATTAAATTGCCGCAACATATTATTCCAACAGGAACATACAAAAACTCAACAACATATATTTCCGAGCATAAATCAACCCGGAGTTACTTTTTCTGAAACAAGTAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGa                                                                                                    <  2:3459151/150‑1 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  >  2:5310001/1‑150 (MQ=255)
                                                cAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAat                                                                                                  <  1:95936/150‑1 (MQ=255)
                                                 aaataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGACGTCTGGAata                                                                                                 >  2:4991637/1‑150 (MQ=255)
                                                  aataaatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAAGATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAatat                                                                                                <  2:215211/150‑1 (MQ=255)
                                                      aatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                            >  2:2471574/1‑150 (MQ=255)
                                                      aatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                            >  2:2734603/1‑150 (MQ=255)
                                                      aatTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCa                                                                                            <  1:4797905/150‑1 (MQ=255)
                                                         tGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAttt                                                                                         <  2:3914324/150‑1 (MQ=255)
                                                          gCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCAtttt                                                                                        <  1:4092919/150‑1 (MQ=255)
                                                           ccGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTc                                                                                       <  2:1763142/150‑1 (MQ=255)
                                                              cAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAAt                                                                                    >  2:4054976/1‑150 (MQ=255)
                                                                aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATcc                                                                                  <  2:3371516/150‑1 (MQ=255)
                                                                aCATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATcc                                                                                  >  1:5111880/1‑150 (MQ=255)
                                                                  atatTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAg                                                                                >  1:2982819/1‑150 (MQ=255)
                                                                    attatACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTa                                                                              >  2:4890592/1‑150 (MQ=255)
                                                                        taCCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAgg                                                                          <  1:3529374/150‑1 (MQ=255)
                                                                          ccAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgt                                                                        <  1:3192022/150‑1 (MQ=255)
                                                                           cAACAGGAACATACAAAAACTCAACAACTATTATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATTAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtg                                                                       <  1:1761086/150‑1 (MQ=255)
                                                                           cAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtg                                                                       <  1:3421140/150‑1 (MQ=255)
                                                                            aaCAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGgtgt                                                                      >  2:3773551/1‑150 (MQ=255)
                                                                              cAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGttt                                                                    <  1:1223241/150‑1 (MQ=255)
                                                                              cAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGttt                                                                    <  2:1877327/150‑1 (MQ=255)
                                                                               aGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTg                                                                   >  1:3795729/1‑150 (MQ=255)
                                                                                 gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGcc                                                                 >  2:5202356/1‑150 (MQ=255)
                                                                                 gAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGcc                                                                 <  1:143074/150‑1 (MQ=255)
                                                                                    cATACACAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCt                                                              <  2:4319146/150‑1 (MQ=255)
                                                                                    cATAAAAAAACTCAACAACAAAAAATTACGAGAAAAAATCAACCCGGAGTTACATATACTGAAAAAAGAAAAATGACGAGAAAAGGTACAACATAAGGAACAGAAGTATTGAAAATAACAATTTCAATCAAGTAAATCGTGttatcccaa                                                              >  2:2406676/1‑143 (MQ=255)
                                                                                      tACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTgg                                                            <  1:3952286/150‑1 (MQ=255)
                                                                                       aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGACTATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTggg                                                           >  2:4531234/1‑150 (MQ=255)
                                                                                       aCAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTggg                                                           >  2:4650720/1‑150 (MQ=255)
                                                                                        cAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGc                                                          >  2:644309/1‑150 (MQ=255)
                                                                                           aaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATGAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTa                                                       >  2:228370/1‑150 (MQ=255)
                                                                                           aaaCTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCAAGTAAAGGGTGTTTGCACCTGGGCGTa                                                       >  2:1544674/1‑150 (MQ=255)
                                                                                                caacaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTa                                                  >  2:442388/1‑150 (MQ=255)
                                                                                                  acaacaaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTaaa                                                <  2:74534/150‑1 (MQ=255)
                                                                                                     acaaATGTTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGc                                             <  2:4222758/150‑1 (MQ=255)
                                                                                                      caaATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCg                                            >  1:2677421/1‑150 (MQ=255)
                                                                                                         atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                         <  2:2235251/150‑1 (MQ=255)
                                                                                                         atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                         <  1:4493763/150‑1 (MQ=255)
                                                                                                         atatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTg                                         <  2:4738885/150‑1 (MQ=255)
                                                                                                          tatTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTga                                        <  1:5079419/150‑1 (MQ=255)
                                                                                                           atTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgag                                       >  1:3108225/1‑150 (MQ=255)
                                                                                                            tttCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTgaga                                      >  1:1961073/1‑150 (MQ=255)
                                                                                                             taccGAGCATCAAACCACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGat                                     <  1:4651294/148‑1 (MQ=255)
                                                                                                              tCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGata                                    <  2:1235661/150‑1 (MQ=255)
                                                                                                               ccGAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGatat                                   <  2:4163180/150‑1 (MQ=255)
                                                                                                                 gAGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGc                                 >  1:4228700/1‑150 (MQ=255)
                                                                                                                  aGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCa                                >  1:3671292/1‑150 (MQ=255)
                                                                                                                  aGCATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCa                                >  2:2599585/1‑150 (MQ=255)
                                                                                                                    cATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATc                              >  1:2423849/1‑150 (MQ=255)
                                                                                                                    cATAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATc                              >  1:564335/1‑150 (MQ=255)
                                                                                                                     aTAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCa                             >  1:1960446/1‑150 (MQ=255)
                                                                                                                      tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAg                            <  2:938586/150‑1 (MQ=255)
                                                                                                                      tAAATCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAg                            <  2:853042/150‑1 (MQ=255)
                                                                                                                         aTCAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgctg                         >  2:1083618/1‑150 (MQ=255)
                                                                                                                           cAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGTTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgctgct                       >  2:323382/1‑150 (MQ=255)
                                                                                                                           cAACCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAgctgct                       >  1:2190349/1‑150 (MQ=255)
                                                                                                                            aaCCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCtt                      >  1:2497976/1‑150 (MQ=255)
                                                                                                                            aaCCCGGAGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCtt                      >  1:1621817/1‑150 (MQ=255)
                                                                                                                                   aGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt               >  2:4332121/1‑150 (MQ=255)
                                                                                                                                   aGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt               >  2:3523218/1‑150 (MQ=255)
                                                                                                                                   aGTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGtt               >  2:193680/1‑150 (MQ=255)
                                                                                                                                    gTTACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTc              >  2:2008355/1‑150 (MQ=255)
                                                                                                                                     ttACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCa             <  1:4378134/150‑1 (MQ=255)
                                                                                                                                      tACTTATTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCAt            >  2:28165/1‑150 (MQ=255)
                                                                                                                                       aCTTATTCTGAAACNAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTNAGATATGCATCAGCTGCTTCCCGGTTCATc           >  2:565701/1‑150 (MQ=255)
                                                                                                                                       aCTTATTCTGAAAAAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATc           <  1:4883404/150‑1 (MQ=255)
                                                                                                                                           aTTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCt       <  1:1527380/150‑1 (MQ=255)
                                                                                                                                           aTTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCt       <  1:67198/150‑1 (MQ=255)
                                                                                                                                           aTTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCt       <  1:623403/150‑1 (MQ=255)
                                                                                                                                           aTTCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCt       >  1:1008261/1‑150 (MQ=255)
                                                                                                                                            ttCTGAAACAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCtt      <  1:4858114/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTGCGTGACATTCTCTGGTTAAATTTATGTAATAAAAATTATGCGGCAAATAAATTGCCGCAACATATTATACCAACAGGAACATACAAAAACTCAACAACAAATATTTCCGAGCATAAATCAACCCGGAGTTACTTATTCTGAAGCAAGAAATTTGTCGAGATAAGGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATT  >  CP009273/4350076‑4350369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑