Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,381,504 T→A 100% I28I (ATT→ATA)  orn → oligoribonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,381,5040TA99.3% 957.4 / ‑3.3 292I28I (ATT→ATA) ornoligoribonuclease
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (187/103);  total (187/105)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 292 total aligned reads displayed.

AAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGC  >  CP009273/4381358‑4381650
                                                                                                                                                  |                                                                                                                                                  
   aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACg                                                                                                                                              <  2:2953714/150‑1 (MQ=255)
   aCATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACg                                                                                                                                              <  2:2362894/150‑1 (MQ=255)
    cATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGc                                                                                                                                             <  1:2635142/150‑1 (MQ=255)
     aTCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCt                                                                                                                                            <  2:2657394/150‑1 (MQ=255)
     aTCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCt                                                                                                                                            >  2:2644252/1‑150 (MQ=255)
        tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                         >  2:3390384/1‑150 (MQ=255)
        tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                         <  2:4531695/150‑1 (MQ=255)
        tATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGt                                                                                                                                         <  2:4264301/150‑1 (MQ=255)
         aTGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTg                                                                                                                                        >  2:2509716/1‑150 (MQ=255)
          tGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                       >  2:4952459/1‑150 (MQ=255)
          tGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGa                                                                                                                                       <  2:2229065/150‑1 (MQ=255)
            aTACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGAcc                                                                                                                                     <  1:710128/150‑1 (MQ=255)
             tACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCg                                                                                                                                    >  1:906546/1‑150 (MQ=255)
                    aaTTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCaa                                                                                                                             >  2:457456/1‑150 (MQ=255)
                             tCAATATAGTCACTGTGAATTGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAatat                                                                                                                    <  2:3627491/150‑1 (MQ=255)
                             tCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAatat                                                                                                                    <  2:4119898/150‑1 (MQ=255)
                              cAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                   <  2:4850246/150‑1 (MQ=255)
                              cAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTGGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATAtt                                                                                                                   <  2:1274237/150‑1 (MQ=255)
                               aaTATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                                  <  2:2435106/150‑1 (MQ=255)
                               aaTATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTc                                                                                                                  >  1:3707362/1‑150 (MQ=255)
                                atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                                 >  1:201453/1‑150 (MQ=255)
                                atatAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCt                                                                                                                 >  1:4814996/1‑150 (MQ=255)
                                 tataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTg                                                                                                                >  2:1053409/1‑150 (MQ=255)
                                  ataGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTgg                                                                                                               <  2:4286783/150‑1 (MQ=255)
                                    aGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCa                                                                                                             >  1:406599/1‑150 (MQ=255)
                                            tgAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGcc                                                                                                     >  2:4085078/1‑150 (MQ=255)
                                              aaTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGa                                                                                                   >  2:3558589/1‑150 (MQ=255)
                                               aTGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAc                                                                                                  <  2:4942334/150‑1 (MQ=255)
                                                tgtgTGGAAAATATCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTATGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTTGCAGAAGGGCCGAcc                                                                                                 <  1:2328075/147‑1 (MQ=255)
                                                tGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAcc                                                                                                 >  1:2773514/1‑150 (MQ=255)
                                                tGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGAcc                                                                                                 >  2:4762748/1‑150 (MQ=255)
                                                 gggTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCa                                                                                                >  1:2165020/1‑150 (MQ=255)
                                                  ggTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCAt                                                                                               <  1:103937/150‑1 (MQ=255)
                                                    tGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTg                                                                                             <  1:3315043/150‑1 (MQ=255)
                                                     ggAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGc                                                                                            >  2:3450072/1‑150 (MQ=255)
                                                      gAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAGGGGCCGACCATTGCa                                                                                           >  2:3610941/1‑150 (MQ=255)
                                                      gAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCAACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCa                                                                                           >  1:4959831/1‑150 (MQ=255)
                                                       aaaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAg                                                                                          >  1:836958/1‑150 (MQ=255)
                                                        aaaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGt                                                                                         >  1:2588438/1‑150 (MQ=255)
                                                         aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTa                                                                                        >  2:3740268/1‑150 (MQ=255)
                                                         aaTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTa                                                                                        <  2:2163706/150‑1 (MQ=255)
                                                          aTAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTac                                                                                       <  1:4097458/150‑1 (MQ=255)
                                                           tAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTaca                                                                                      >  1:2896393/1‑150 (MQ=255)
                                                            aGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTacac                                                                                     >  2:4429522/1‑150 (MQ=255)
                                                             gCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACAcc                                                                                    <  2:2857937/150‑1 (MQ=255)
                                                              caacagagCCAATGAAAACAACCTGATTTGTATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTAATGAGATAGCCACGCTGGTGACCGATGCCAACATGAATATTCTGGCAGAAGGGCCGACCATTGCAGAACACCa                                                                                   >  2:111022/8‑150 (MQ=255)
                                                               aTGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAg                                                                                  >  1:4931329/1‑150 (MQ=255)
                                                               aTGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAg                                                                                  >  1:4428346/1‑150 (MQ=255)
                                                                tGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGt                                                                                 >  2:3208069/1‑150 (MQ=255)
                                                                  aGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCt                                                                               <  2:683247/150‑1 (MQ=255)
                                                                      ccAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatg                                                                           >  2:1511302/1‑150 (MQ=255)
                                                                      caaaTGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGAAGAAGGGCCGACCATTGCTGTACACCAGTCtgatg                                                                           <  1:1105600/148‑1 (MQ=255)
                                                                       cAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCtgatga                                                                          >  2:2730548/1‑150 (MQ=255)
                                                                              aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                   >  2:3966668/1‑150 (MQ=255)
                                                                              aacaacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTg                                                                   >  1:4643619/1‑150 (MQ=255)
                                                                                caacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgc                                                                 >  2:1027270/1‑150 (MQ=255)
                                                                                 aacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcg                                                                >  2:1750211/1‑150 (MQ=255)
                                                                                 aacCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGgcg                                                                >  2:2015112/1‑150 (MQ=255)
                                                                                      gATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgat                                                           <  1:661635/150‑1 (MQ=255)
                                                                                       aTTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatg                                                          >  1:2824117/1‑150 (MQ=255)
                                                                                        tttGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatgg                                                         <  1:1839344/150‑1 (MQ=255)
                                                                                          tGGATCGATCTTGAGATGAACGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGACACGCTGGTGACCGATGACAACCTGAATATTCTGGCAGAAGGGCCGACAATTGCAGTACACCAGTCTGATGAACATCTGTCGCTtatggat                                                       >  2:4355853/1‑150 (MQ=255)
                                                                                           ggATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggatg                                                      >  2:2253516/1‑150 (MQ=255)
                                                                                           ggATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTgatggatg                                                      <  2:1707631/150‑1 (MQ=255)
                                                                                            gatcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGa                                                     >  1:5073342/1‑150 (MQ=255)
                                                                                             atcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGAc                                                    <  1:2432439/150‑1 (MQ=255)
                                                                                              tcgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACt                                                   >  1:1930953/1‑150 (MQ=255)
                                                                                               cgatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTg                                                  >  1:2961142/1‑150 (MQ=255)
                                                                                                gatcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTgg                                                 >  2:350484/1‑150 (MQ=255)
                                                                                                 atcTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGa                                                >  1:526258/1‑150 (MQ=255)
                                                                                                   cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAAc                                              <  1:1616330/150‑1 (MQ=255)
                                                                                                   cTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGAGAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAAc                                              <  2:323049/150‑1 (MQ=255)
                                                                                                    ttGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACg                                             >  2:5073508/1‑150 (MQ=255)
                                                                                                     tGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGCTGCCAACCTGAAGATTCTGGAAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGt                                            >  2:1861623/1‑150 (MQ=255)
                                                                                                      gagaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                           <  1:3210445/150‑1 (MQ=255)
                                                                                                      gagaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                           >  1:1179071/1‑150 (MQ=255)
                                                                                                      gagaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTg                                           >  2:3813747/1‑150 (MQ=255)
                                                                                                       agaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgc                                          >  2:4670694/1‑150 (MQ=255)
                                                                                                        gaTGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTgcg                                         >  2:3167802/1‑150 (MQ=255)
                                                                                                          tGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCa                                       >  1:377376/1‑150 (MQ=255)
                                                                                                               ggTCTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCAt                                  >  1:641676/1‑150 (MQ=255)
                                                                                                                  cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                               <  2:2692259/150‑1 (MQ=255)
                                                                                                                  cTGGATCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATAcc                               <  1:4894823/150‑1 (MQ=255)
                                                                                                                      aTCCCGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCa                           >  2:5123718/1‑150 (MQ=255)
                                                                                                                         ccGAGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACAGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCg                        <  2:3033385/150‑1 (MQ=255)
                                                                                                                           gAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCggg                      <  1:2137535/150‑1 (MQ=255)
                                                                                                                            aGCGCGATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGc                     >  1:4187351/1‑150 (MQ=255)
                                                                                                                               gcgATCGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTgg                  <  2:15752/150‑1 (MQ=255)
                                                                                                                                    cGCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTagag             >  1:171739/1‑150 (MQ=255)
                                                                                                                                     gCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgc            <  1:4719441/150‑1 (MQ=255)
                                                                                                                                     gCATTATTGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgc            <  1:383587/150‑1 (MQ=255)
                                                                                                                                       attattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAgcgc          >  1:4268341/1‑150 (MQ=255)
                                                                                                                                        ttattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGcgcg         >  2:564197/1‑150 (MQ=255)
                                                                                                                                        ttattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGcgcg         >  2:765887/1‑150 (MQ=255)
                                                                                                                                         tattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACTCATACCGCCAGCGGGCTGGTAGAGCGCGt        >  1:1278171/1‑150 (MQ=255)
                                                                                                                                         tattGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGt        <  1:1750211/150‑1 (MQ=255)
                                                                                                                                          attGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTg       >  2:4605020/1‑150 (MQ=255)
                                                                                                                                          attGAGATAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTg       >  1:3520805/1‑150 (MQ=255)
                                                                                                                                             gagaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGaaa    >  1:3147915/1‑150 (MQ=255)
                                                                                                                                               gaTAGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGc  <  1:22572/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAACATCTATGATACACGCAATTGTGGATCAATATAGTCACTGTGAATGGGTGGAAAATAGCATGAGTGCCAATGAAAACAACCTGATTTGGATCGATCTTGAGATGACCGGTCTGGATCCCGAGCGCGATCGCATTATTGAGATTGCCACGCTGGTGACCGATGCCAACCTGAATATTCTGGCAGAAGGGCCGACCATTGCAGTACACCAGTCTGATGAACAGCTGGCGCTGATGGATGACTGGAACGTGCGCACCCATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGC  >  CP009273/4381358‑4381650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑