Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,406,553 C→A 100% R12L (CGG→CTG)  bsmA ← bioflm peroxide resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,406,5530CA98.2% 1177.6 / 3.9 372R12L (CGG→CTG) bsmAbioflm peroxide resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base A (158/207);  total (158/214)
Fisher's exact test for biased strand distribution p-value = 2.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 372 total aligned reads displayed.

GTGCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGA  >  CP009273/4406406‑4406699
                                                                                                                                                   |                                                                                                                                                  
       cGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATaa                                                                                                                                           >  2:4117507/1‑150 (MQ=255)
        gCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAAc                                                                                                                                          >  2:3563924/1‑150 (MQ=255)
        gCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAAc                                                                                                                                          <  1:3638310/150‑1 (MQ=255)
           aCCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCt                                                                                                                                       <  1:3953386/150‑1 (MQ=255)
           aCCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCt                                                                                                                                       <  1:4813088/150‑1 (MQ=255)
            ccTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTa                                                                                                                                      >  2:3699621/1‑150 (MQ=255)
               aTTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTa                                                                                                                                   >  1:4784197/1‑150 (MQ=255)
                 tCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACg                                                                                                                                 <  1:2447490/150‑1 (MQ=255)
                   gCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGtt                                                                                                                               <  2:2023324/150‑1 (MQ=255)
                    cTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGACCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGttt                                                                                                                              <  2:1321348/150‑1 (MQ=255)
                       tAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCt                                                                                                                           >  2:2587721/1‑150 (MQ=255)
                          cccTTGCGTCTGGTCGCGGCGAATCTCGTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCt                                                                                                                        <  1:2094508/150‑1 (MQ=255)
                           ccTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTa                                                                                                                       <  2:2559401/150‑1 (MQ=255)
                            cTTGCGTCGGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTaa                                                                                                                      >  1:1406887/1‑150 (MQ=255)
                             ttGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAAc                                                                                                                     <  2:1692615/150‑1 (MQ=255)
                              tGCGTCTGGTCGCTGCTAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTTCAGTGCGCTACAGGCAATTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTTTTACGTTTCCTGCTAAcc                                                                                                                    <  2:4375417/150‑1 (MQ=255)
                              tGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAAcc                                                                                                                    >  1:5208643/1‑150 (MQ=255)
                                 gTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAta                                                                                                                 >  2:474968/1‑150 (MQ=255)
                                  tcgggTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtag                                                                                                                <  2:724784/147‑1 (MQ=255)
                                  tCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtag                                                                                                                <  2:3941433/150‑1 (MQ=255)
                                     ggTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgt                                                                                                             <  1:4647712/150‑1 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                            <  1:147969/150‑1 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                            <  1:1920231/150‑1 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                            <  2:1803180/150‑1 (MQ=255)
                                      gTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgta                                                                                                            <  1:2184832/150‑1 (MQ=255)
                                       tCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtag                                                                                                           <  2:1026850/150‑1 (MQ=255)
                                         gcggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCAtagtgtagtg                                                                                                         >  2:3464219/1‑150 (MQ=255)
                                          cggcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGc                                                                                                        <  2:4671645/150‑1 (MQ=255)
                                            gcgAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCt                                                                                                      <  2:671769/150‑1 (MQ=255)
                                              gAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCATGCTAACCATAGTGTAGTGCCtta                                                                                                    >  1:2703118/1‑150 (MQ=255)
                                                 tctcTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCttatta                                                                                                 >  1:3591234/1‑150 (MQ=255)
                                                  ctctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTaa                                                                                                >  2:3283933/1‑150 (MQ=255)
                                                   tctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAt                                                                                               <  2:3176455/150‑1 (MQ=255)
                                                   tctTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAAt                                                                                               <  2:245907/150‑1 (MQ=255)
                                                       gCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTaa                                                                                           >  1:3320128/1‑150 (MQ=255)
                                                          gATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACtt                                                                                        >  2:5175301/1‑150 (MQ=255)
                                                               tCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTgggg                                                                                   <  2:1834092/150‑1 (MQ=255)
                                                                 cGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGa                                                                                 <  1:4678813/150‑1 (MQ=255)
                                                                  gTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGaa                                                                                >  2:3649771/1‑150 (MQ=255)
                                                                  gTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGaa                                                                                <  1:4363882/150‑1 (MQ=255)
                                                                  gTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGaa                                                                                >  2:407202/1‑150 (MQ=255)
                                                                   ttACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAAt                                                                               <  2:4269995/150‑1 (MQ=255)
                                                                   ttACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAAt                                                                               <  1:3089100/150‑1 (MQ=255)
                                                                    tACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATg                                                                              <  2:3001200/150‑1 (MQ=255)
                                                                       tGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTc                                                                           <  1:5178509/150‑1 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTccc                                                                         <  2:2103276/150‑1 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTccc                                                                         <  1:99039/150‑1 (MQ=255)
                                                                         gCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTccc                                                                         <  1:1004425/150‑1 (MQ=255)
                                                                          cGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCg                                                                        <  2:605803/150‑1 (MQ=255)
                                                                            gTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGaa                                                                      >  1:163470/1‑150 (MQ=255)
                                                                             tGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAAt                                                                     <  2:1907455/150‑1 (MQ=255)
                                                                              gctggctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAAtt                                                                    <  1:1057915/150‑1 (MQ=255)
                                                                                   ctgTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAc                                                               <  1:3777748/150‑1 (MQ=255)
                                                                                    tgtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAcc                                                              <  1:104149/150‑1 (MQ=255)
                                                                                    tgtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAcc                                                              <  1:4335204/150‑1 (MQ=255)
                                                                                    tgtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTAcc                                                              <  1:4454529/150‑1 (MQ=255)
                                                                                     gtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCt                                                             <  1:800959/150‑1 (MQ=255)
                                                                                     gtgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCt                                                             <  2:3413871/150‑1 (MQ=255)
                                                                                      tgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTg                                                            <  1:2648816/150‑1 (MQ=255)
                                                                                      tgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTg                                                            <  2:1263510/150‑1 (MQ=255)
                                                                                      tgGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTg                                                            <  1:5262985/150‑1 (MQ=255)
                                                                                       ggCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTgg                                                           >  2:889211/1‑150 (MQ=255)
                                                                                       ggCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTgg                                                           <  2:3496343/150‑1 (MQ=255)
                                                                                          gTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAc                                                        >  1:3443537/1‑150 (MQ=255)
                                                                                           tACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTAcg                                                       <  1:2572658/150‑1 (MQ=255)
                                                                                                 gCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGAttt                                                 <  1:2927419/150‑1 (MQ=255)
                                                                                                    gTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCg                                              <  1:3532994/150‑1 (MQ=255)
                                                                                                     tGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGa                                             <  2:5108173/150‑1 (MQ=255)
                                                                                                       cgcTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAAt                                           <  1:1566597/150‑1 (MQ=255)
                                                                                                         cTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGa                                         <  2:2958968/150‑1 (MQ=255)
                                                                                                         cTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGa                                         >  1:4975598/1‑150 (MQ=255)
                                                                                                          tACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGaa                                        <  1:1304787/150‑1 (MQ=255)
                                                                                                             aGGCACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAAc                                     >  1:1230258/1‑150 (MQ=255)
                                                                                                                cACTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTg                                  <  1:4117507/150‑1 (MQ=255)
                                                                                                                  cTTAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGAc                                <  2:4347825/150‑1 (MQ=255)
                                                                                                                   ttAACATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACg                               <  2:5208647/150‑1 (MQ=255)
                                                                                                                       cATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAg                           >  1:2306050/1‑150 (MQ=255)
                                                                                                                       cATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAg                           >  1:3037539/1‑150 (MQ=255)
                                                                                                                       cATCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAg                           >  2:264355/1‑150 (MQ=255)
                                                                                                                         tCAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCg                         <  1:4590075/150‑1 (MQ=255)
                                                                                                                          cAACACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGt                        >  1:949076/1‑150 (MQ=255)
                                                                                                                           aaCACCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTa                       <  2:2820564/150‑1 (MQ=255)
                                                                                                                             cacCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGACTGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAc                     >  1:3519979/1‑150 (MQ=255)
                                                                                                                             cacCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAc                     <  2:2166551/150‑1 (MQ=255)
                                                                                                                             cacCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAc                     >  2:4958652/1‑150 (MQ=255)
                                                                                                                              acCAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAAcc                    <  2:3074108/150‑1 (MQ=255)
                                                                                                                                cAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCca                  >  1:3157864/1‑150 (MQ=255)
                                                                                                                                cAATAATAAACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCca                  >  1:175082/1‑150 (MQ=255)
                                                                                                                                    aataaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtc              <  1:667475/150‑1 (MQ=255)
                                                                                                                                     ataaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtct             >  2:1138410/1‑150 (MQ=255)
                                                                                                                                      taaACTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACAtctc            <  2:785883/150‑1 (MQ=255)
                                                                                                                                       aaaCTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTTCGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCa           <  2:5314136/150‑1 (MQ=255)
                                                                                                                                          cTGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACa        <  2:3856625/150‑1 (MQ=255)
                                                                                                                                           tGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAt       >  2:2691405/1‑150 (MQ=255)
                                                                                                                                           tGGCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAt       >  1:5284134/1‑150 (MQ=255)
                                                                                                                                            ggCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTGGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAtt      >  1:197847/1‑150 (MQ=255)
                                                                                                                                            ggCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAtt      >  2:4561356/1‑150 (MQ=255)
                                                                                                                                            ggCAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACAtt      <  1:890869/150‑1 (MQ=255)
                                                                                                                                              cAAACAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTAt    <  1:2253817/150‑1 (MQ=255)
                                                                                                                                                aaCAGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGa  >  2:143912/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGCTTACGCTACCTATTCGCTGTAACCCTTGCGTCTGGTCGCGGCGAATCTCTTGCGGATGGTCCGTTACTGGCGGTGCTGGCTGTGGCGTACCTTGCAGTGCGCTACAGGCACTTAACATCAACACCAATAATAAACTGGCAAACCGGTAAATAACGCTATTACGTTTCCTGCTAACCATAGTGTAGTGCCTTATTAATTTAACTTTGGGGGAATGTTCCCGAATTGTTACCTGGTACGCGATTTTCGAATGAAAACGTGACGAAAGCGTAACCCACATCTCAACATTATGA  >  CP009273/4406406‑4406699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑