Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,410,964 A→C 100% I390L (ATC→CTC)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,410,9640AC99.7% 1126.7 / ‑5.5 283I390L (ATC→CTC) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (152/130);  total (153/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 286 total aligned reads displayed.

GTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATT  >  CP009273/4410817‑4411110
                                                                                                                                                   |                                                                                                                                                  
     cATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAAt                                                                                                                                             >  2:3438610/1‑150 (MQ=255)
        ccTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATNCTCGAAATCtt                                                                                                                                          <  1:2760822/150‑1 (MQ=255)
         cTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCttt                                                                                                                                         >  1:171993/1‑150 (MQ=255)
         cTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCttt                                                                                                                                         >  1:4434416/1‑150 (MQ=255)
          tGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTg                                                                                                                                        <  2:1505133/150‑1 (MQ=255)
           ggTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTgg                                                                                                                                       <  1:535537/150‑1 (MQ=255)
             tCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGtt                                                                                                                                     >  2:572978/1‑150 (MQ=255)
             tCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGtt                                                                                                                                     <  1:5190427/150‑1 (MQ=255)
             tCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGtt                                                                                                                                     <  2:4961563/150‑1 (MQ=255)
              cGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTg                                                                                                                                    <  1:4465515/150‑1 (MQ=255)
                 cTGCGGCTCCTCGATCCTGATTATTCCTGTCTTTATCCCGATGTTCTTCTCTAACGCCACCATCTGCATGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGt                                                                                                                                 <  2:3793665/150‑1 (MQ=255)
                 cTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGt                                                                                                                                 >  2:4041287/1‑150 (MQ=255)
                   gCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCt                                                                                                                               <  2:199700/150‑1 (MQ=255)
                   gCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTATTCTCTAAAGCCAACATCGGAGTGTTCTCTAACCACTTCGTCGGCTGGCGTGCGGCGCTGAAGATTTTTATGGTGATGGGGATGCTCGAAATCTTTGGTTGCTTCt                                                                                                                               >  1:4769915/1‑150 (MQ=255)
                      gCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTggg                                                                                                                            <  1:4242543/150‑1 (MQ=255)
                       ctcctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGc                                                                                                                           >  1:776364/1‑150 (MQ=255)
                        tcctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                          <  2:4478687/150‑1 (MQ=255)
                        tcctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCg                                                                                                                          >  1:1787669/1‑150 (MQ=255)
                         cctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAAGCTGTGGTTGCGTCTGGGCgg                                                                                                                         >  2:1711195/1‑150 (MQ=255)
                         cctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGAGTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCGGGGCgg                                                                                                                         >  2:1402022/1‑150 (MQ=255)
                          ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGt                                                                                                                        <  2:5300783/150‑1 (MQ=255)
                          ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGt                                                                                                                        <  2:1181755/150‑1 (MQ=255)
                            cGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGa                                                                                                                      >  1:1643783/1‑150 (MQ=255)
                             gATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaa                                                                                                                     >  1:1465164/1‑150 (MQ=255)
                              aTCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGaaa                                                                                                                    >  2:3710147/1‑150 (MQ=255)
                              aTCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTGTGGTTGCGTCTGGGCGGTGaaa                                                                                                                    >  1:768306/1‑150 (MQ=255)
                                 cTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATATTTGGTTGCGTCTGGGCGGTGAAACTc                                                                                                                 >  1:210945/1‑150 (MQ=255)
                                     ttattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCg                                                                                                             >  1:3575429/1‑150 (MQ=255)
                                     ttattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCg                                                                                                             <  2:3929145/150‑1 (MQ=255)
                                       attCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGt                                                                                                           <  2:1921201/150‑1 (MQ=255)
                                        atCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGACGATTTGTCTGGTGATGGGGATGCTCGAAATCGTTGGTTGCGTCTGGGCGGTGAAACTCACCGGtc                                                                                                          >  2:4568263/2‑149 (MQ=255)
                                           cTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGa                                                                                                       >  1:1422887/1‑150 (MQ=255)
                                            tGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAg                                                                                                      >  2:3510092/1‑150 (MQ=255)
                                              gCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTg                                                                                                    <  2:1129954/150‑1 (MQ=255)
                                               cTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                   >  2:5149356/1‑150 (MQ=255)
                                               cTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAAATCACCGGTATGAGTGc                                                                                                   >  1:2117316/1‑150 (MQ=255)
                                                  tATCCCGAGGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAACTCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTg                                                                                                >  2:1581081/1‑150 (MQ=255)
                                                   aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTgg                                                                                               >  1:3167236/1‑150 (MQ=255)
                                                   aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTgg                                                                                               >  1:1323755/1‑150 (MQ=255)
                                                   aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTgg                                                                                               >  1:5138831/1‑150 (MQ=255)
                                                     cccGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGAt                                                                                             <  2:2509951/150‑1 (MQ=255)
                                                     cccGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGAt                                                                                             >  2:4727300/1‑150 (MQ=255)
                                                      ccGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATg                                                                                            <  2:2670555/150‑1 (MQ=255)
                                                       cGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATgg                                                                                           <  1:846902/150‑1 (MQ=255)
                                                        gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATggg                                                                                          >  1:340730/1‑150 (MQ=255)
                                                         aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGc                                                                                         >  1:1620932/1‑150 (MQ=255)
                                                         aTGTTCTTCTCTAACGCCACCATCGGCGTGGTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTCTGAGTGCCTGGATGGGc                                                                                         >  1:4651850/1‑150 (MQ=255)
                                                            ttcttcTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATg                                                                                      <  2:3296810/150‑1 (MQ=255)
                                                            ttcttcTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATg                                                                                      <  2:278577/150‑1 (MQ=255)
                                                             tcttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATgg                                                                                     >  1:2797549/1‑150 (MQ=255)
                                                              cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGc                                                                                    <  2:4218454/150‑1 (MQ=255)
                                                              cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGc                                                                                    >  1:3450405/1‑150 (MQ=255)
                                                              cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGc                                                                                    >  2:2400918/1‑150 (MQ=255)
                                                               ttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCg                                                                                   >  2:2231105/1‑150 (MQ=255)
                                                                  tctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGAc                                                                                >  1:2589879/1‑150 (MQ=255)
                                                                   ctAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACt                                                                               >  1:2408829/1‑150 (MQ=255)
                                                                    tAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTg                                                                              >  2:744653/1‑150 (MQ=255)
                                                                     aaCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTgg                                                                             <  1:97525/150‑1 (MQ=255)
                                                                      aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGt                                                                            <  1:3294630/150‑1 (MQ=255)
                                                                        gCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCg                                                                          >  2:3060479/1‑150 (MQ=255)
                                                                          caccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGAt                                                                        <  2:1321727/150‑1 (MQ=255)
                                                                          caccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGAt                                                                        >  1:2898646/1‑150 (MQ=255)
                                                                            ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTc                                                                      <  2:1814101/150‑1 (MQ=255)
                                                                              aTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTg                                                                    <  1:1237135/150‑1 (MQ=255)
                                                                              aTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCAGGATGGGCATGGCGGACTGGTCGATTCTg                                                                    <  1:435836/150‑1 (MQ=255)
                                                                                          gCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatg                                                        >  1:215549/1‑150 (MQ=255)
                                                                                            tAACCACGTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatgat                                                      <  2:2021516/150‑1 (MQ=255)
                                                                                                 aCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAg                                                 <  1:4539843/150‑1 (MQ=255)
                                                                                                   ttCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGc                                               <  1:110879/150‑1 (MQ=255)
                                                                                                    tCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGct                                              <  1:3717827/150‑1 (MQ=255)
                                                                                                      ggcggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttc                                            >  1:2507334/1‑150 (MQ=255)
                                                                                                       gcggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttct                                           <  1:4858428/150‑1 (MQ=255)
                                                                                                       gcggcTGGCGTGCGGCGCTGAAGATTTGTCGGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttct                                           <  2:2446970/150‑1 (MQ=255)
                                                                                                        cggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGcttctt                                          <  2:2295009/150‑1 (MQ=255)
                                                                                                          gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTtcttct                                        <  2:1746583/150‑1 (MQ=255)
                                                                                                          gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTtcttct                                        <  1:4672103/150‑1 (MQ=255)
                                                                                                           cTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTtctc                                       >  1:1735415/1‑150 (MQ=255)
                                                                                                           cTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTtctc                                       >  1:3828266/1‑150 (MQ=255)
                                                                                                            tGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTcc                                      >  1:3624207/1‑150 (MQ=255)
                                                                                                             ggCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCa                                     <  2:3313497/150‑1 (MQ=255)
                                                                                                                gTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCg                                  <  2:3885283/150‑1 (MQ=255)
                                                                                                                      cgcTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCg                            >  2:2638706/1‑150 (MQ=255)
                                                                                                                      cgcTGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCg                            >  2:3363881/1‑150 (MQ=255)
                                                                                                                         tGAAGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCt                         <  1:4763751/150‑1 (MQ=255)
                                                                                                                          gAAGATTTGTCTGGTGATGGGGATGCTCGAAATCATTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCtt                        <  1:1402022/150‑1 (MQ=255)
                                                                                                                            aGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTa                      >  2:482716/1‑150 (MQ=255)
                                                                                                                            aGATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTa                      <  1:1849645/150‑1 (MQ=255)
                                                                                                                             gATTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTAt                     <  2:1039118/150‑1 (MQ=255)
                                                                                                                              aTTTGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATg                    >  2:831823/1‑150 (MQ=255)
                                                                                                                               tttGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATgg                   <  1:1008517/150‑1 (MQ=255)
                                                                                                                               tttGTCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATgg                   <  1:2354618/150‑1 (MQ=255)
                                                                                                                                   tCTGGTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGt               >  1:4154846/1‑150 (MQ=255)
                                                                                                                                      ggTGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcat            >  1:3509250/1‑150 (MQ=255)
                                                                                                                                        tGATGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGtcatca          >  1:2227839/1‑150 (MQ=255)
                                                                                                                                          aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCAtt        <  2:3575429/150‑1 (MQ=255)
                                                                                                                                          aTGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCAtt        <  2:2111788/150‑1 (MQ=255)
                                                                                                                                           tGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg       <  2:2344549/150‑1 (MQ=255)
                                                                                                                                           tGGGGATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg       >  1:1906362/1‑150 (MQ=255)
                                                                                                                                             gggATGCTCGAAATCTTTGGGTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTa     <  1:3849407/150‑1 (MQ=255)
                                                                                                                                              ggATGCTCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTaa    <  1:3988529/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATT  >  CP009273/4410817‑4411110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑